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Protein

Seed trypsin/chymotrypsin inhibitor IVB

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of trypsin and of chymotrypsin. May function as a natural phytochemical defense against predators.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 172Reactive bond for trypsin
Sitei42 – 432Reactive bond for chymotrypsin

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI12.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Seed trypsin/chymotrypsin inhibitor IVB
Short name:
PSTI-IVB
Cleaved into the following chain:
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7272Seed trypsin/chymotrypsin inhibitor IVBPRO_0000003275Add
BLAST
Chaini1 – 6363Seed trypsin/chymotrypsin inhibitor IIPRO_0000003276Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi8 ↔ 61Combined sources1 Publication
Disulfide bondi9 ↔ 24Combined sources1 Publication
Disulfide bondi12 ↔ 57Combined sources1 Publication
Disulfide bondi14 ↔ 22Combined sources1 Publication
Disulfide bondi31 ↔ 38Combined sources1 Publication
Disulfide bondi35 ↔ 50Combined sources1 Publication
Disulfide bondi40 ↔ 48Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Seed.

Developmental stagei

During desiccation stage of seed development increasing activity seems to be associated with appearance of isoform (PSTI II) which has a stronger affinity for trypsin.

Structurei

Secondary structure

1
72
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 198Combined sources
Beta strandi22 – 243Combined sources
Beta strandi27 – 304Combined sources
Beta strandi36 – 4510Combined sources
Beta strandi47 – 504Combined sources
Beta strandi53 – 564Combined sources
Beta strandi61 – 633Combined sources
Beta strandi67 – 704Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PBIX-ray2.70A/B1-72[»]
ProteinModelPortaliP56679.
SMRiP56679. Positions 5-72.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56679.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 1 hit.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GDDVKSACCD TCLCTKSNPP TCRCVDVGET CHSACLSCIC AYSNPPKCQC
60 70
FDTQKFCYKA CHNSELEEVI KN
Length:72
Mass (Da):7,864
Last modified:May 30, 2000 - v2
Checksum:iF12E71FAAD9A4BF7
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PBIX-ray2.70A/B1-72[»]
ProteinModelPortaliP56679.
SMRiP56679. Positions 5-72.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI12.018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP56679.

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 1 hit.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Trypsin inhibitor polymorphism: multigene family expression and posttranslational modification."
    Quillien L., Ferrasson E., Molle D., Gueguen J.
    J. Protein Chem. 16:195-203(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Strain: cv. Frilene.
    Tissue: Seed.
  2. "Dimeric crystal structure of a Bowman-Birk protease inhibitor from pea seeds."
    Li de la Sierra I., Quillien L., Flecker P., Gueguen J., Brunie S.
    J. Mol. Biol. 285:1195-1207(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS), DISULFIDE BONDS.
    Tissue: Seed.

Entry informationi

Entry nameiIBBB_PEA
AccessioniPrimary (citable) accession number: P56679
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 30, 2000
Last modified: January 20, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.