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Protein

Suppressor of tumorigenicity 14 protein homolog

Gene

St14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degrades extracellular matrix. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (By similarity). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (By similarity).By similarity

Catalytic activityi

Cleaves various synthetic substrates with Arg or Lys at the P1 position and prefers small side-chain amino acids, such as Ala and Gly, at the P2 position.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei656Charge relay systemBy similarity1
Active sitei711Charge relay systemBy similarity1
Active sitei805Charge relay systemBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: MGI

GO - Biological processi

  • cell migration Source: MGI
  • epithelial cell morphogenesis involved in placental branching Source: MGI
  • keratinocyte differentiation Source: UniProtKB
  • neural tube closure Source: MGI
  • proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-6809371. Formation of the cornified envelope.

Protein family/group databases

MEROPSiS01.302.

Names & Taxonomyi

Protein namesi
Recommended name:
Suppressor of tumorigenicity 14 protein homolog (EC:3.4.21.109)
Alternative name(s):
Epithin
Serine protease 14
Gene namesi
Name:St14
Synonyms:Prss14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1338881. St14.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55CytoplasmicSequence analysisAdd BLAST55
Transmembranei56 – 76Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini77 – 855ExtracellularSequence analysisAdd BLAST779

GO - Cellular componenti

  • basolateral plasma membrane Source: Ensembl
  • extracellular exosome Source: MGI
  • extracellular region Source: MGI
  • extracellular space Source: Ensembl
  • extrinsic component of plasma membrane Source: MGI
  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000887131 – 855Suppressor of tumorigenicity 14 protein homologAdd BLAST855

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineCombined sources1
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi214 ↔ 244By similarity
Glycosylationi302N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi340 ↔ 366By similarity
Glycosylationi365N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi397 ↔ 410By similarity
Glycosylationi421N-linked (GlcNAc...)1 Publication1
Disulfide bondi453 ↔ 464By similarity
Disulfide bondi459 ↔ 477By similarity
Disulfide bondi471 ↔ 486By similarity
Disulfide bondi488 ↔ 501By similarity
Glycosylationi489N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi496 ↔ 514By similarity
Disulfide bondi508 ↔ 523By similarity
Disulfide bondi525 ↔ 537By similarity
Disulfide bondi532 ↔ 550By similarity
Disulfide bondi544 ↔ 559By similarity
Disulfide bondi567 ↔ 579By similarity
Disulfide bondi574 ↔ 593By similarity
Disulfide bondi587 ↔ 602By similarity
Disulfide bondi641 ↔ 657By similarity
Glycosylationi772N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi776 ↔ 790By similarity
Disulfide bondi801 ↔ 830By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP56677.
PaxDbiP56677.
PeptideAtlasiP56677.
PRIDEiP56677.

PTM databases

iPTMnetiP56677.
PhosphoSitePlusiP56677.

Expressioni

Tissue specificityi

Highly expressed in intestine, kidney, lung, and thymus. Not expressed in skeletal muscle, liver, heart, testis and brain.

Gene expression databases

BgeeiENSMUSG00000031995.
ExpressionAtlasiP56677. baseline and differential.
GenevisibleiP56677. MM.

Interactioni

Subunit structurei

Interacts with CDCP1. May interact with TMEFF1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034478.

Structurei

3D structure databases

ProteinModelPortaliP56677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 203SEAPROSITE-ProRule annotationAdd BLAST118
Domaini214 – 331CUB 1PROSITE-ProRule annotationAdd BLAST118
Domaini340 – 444CUB 2PROSITE-ProRule annotationAdd BLAST105
Domaini451 – 488LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST38
Domaini489 – 522LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST34
Domaini523 – 561LDL-receptor class A 3PROSITE-ProRule annotationAdd BLAST39
Domaini565 – 604LDL-receptor class A 4PROSITE-ProRule annotationAdd BLAST40
Domaini615 – 854Peptidase S1PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 4 LDL-receptor class A domains.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SEA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000136851.
HOVERGENiHBG012556.
InParanoidiP56677.
KOiK08670.
OMAiMMCVGFL.
OrthoDBiEOG091G0DF7.
PhylomeDBiP56677.
TreeFamiTF330647.

Family and domain databases

CDDicd00041. CUB. 2 hits.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
4.10.400.10. 4 hits.
InterProiIPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR017051. Peptidase_S1A_matripase.
IPR000082. SEA_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 4 hits.
PF01390. SEA. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF036370. ST14. 1 hit.
PRINTSiPR00261. LDLRECEPTOR.
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 4 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57424. SSF57424. 4 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 3 hits.
PS50068. LDLRA_2. 4 hits.
PS50024. SEA. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSNRGRKAG GGSQDFGAGL KYNSRLENMN GFEEGVEFLP ANNAKKVEKR
60 70 80 90 100
GPRRWVVLVA VLFSFLLLSL MAGLLVWHFH YRNVRVQKVF NGHLRITNEI
110 120 130 140 150
FLDAYENSTS TEFISLASQV KEALKLLYNE VPVLGPYHKK SAVTAFSEGS
160 170 180 190 200
VIAYYWSEFS IPPHLAEEVD RAMAVERVVT LPPRARALKS FVLTSVVAFP
210 220 230 240 250
IDPRMLQRTQ DNSCSFALHA HGAAVTRFTT PGFPNSPYPA HARCQWVLRG
260 270 280 290 300
DADSVLSLTF RSFDVAPCDE HGSDLVTVYD SLSPMEPHAV VRLCGTFSPS
310 320 330 340 350
YNLTFLSSQN VFLVTLITNT DRRHPGFEAT FFQLPKMSSC GGFLSDTQGT
360 370 380 390 400
FSSPYYPGHY PPNINCTWNI KVPNNRNVKV RFKLFYLVDP NVPVGSCTKD
410 420 430 440 450
YVEINGEKYC GERSQFVVSS NSSKITVHFH SDHSYTDTGF LAEYLSYDSN
460 470 480 490 500
DPCPGMFMCK TGRCIRKELR CDGWADCPDY SDERYCRCNA THQFTCKNQF
510 520 530 540 550
CKPLFWVCDS VNDCGDGSDE EGCSCPAGSF KCSNGKCLPQ SQKCNGKDNC
560 570 580 590 600
GDGSDEASCD SVNVVSCTKY TYRCQNGLCL SKGNPECDGK TDCSDGSDEK
610 620 630 640 650
NCDCGLRSFT KQARVVGGTN ADEGEWPWQV SLHALGQGHL CGASLISPDW
660 670 680 690 700
LVSAAHCFQD DKNFKYSDYT MWTAFLGLLD QSKRSASGVQ ELKLKRIITH
710 720 730 740 750
PSFNDFTFDY DIALLELEKS VEYSTVVRPI CLPDATHVFP AGKAIWVTGW
760 770 780 790 800
GHTKEGGTGA LILQKGEIRV INQTTCEDLM PQQITPRMMC VGFLSGGVDS
810 820 830 840 850
CQGDSGGPLS SAEKDGRMFQ AGVVSWGEGC AQRNKPGVYT RLPVVRDWIK

EHTGV
Length:855
Mass (Da):94,655
Last modified:May 4, 2001 - v2
Checksum:i4F10E84DA2146DD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042822 mRNA. Translation: AAD02230.3.
BC005496 mRNA. Translation: AAH05496.1.
CCDSiCCDS22948.1.
RefSeqiNP_035306.2. NM_011176.4.
UniGeneiMm.243926.

Genome annotation databases

EnsembliENSMUST00000034478; ENSMUSP00000034478; ENSMUSG00000031995.
GeneIDi19143.
KEGGimmu:19143.
UCSCiuc009ori.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042822 mRNA. Translation: AAD02230.3.
BC005496 mRNA. Translation: AAH05496.1.
CCDSiCCDS22948.1.
RefSeqiNP_035306.2. NM_011176.4.
UniGeneiMm.243926.

3D structure databases

ProteinModelPortaliP56677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034478.

Protein family/group databases

MEROPSiS01.302.

PTM databases

iPTMnetiP56677.
PhosphoSitePlusiP56677.

Proteomic databases

EPDiP56677.
PaxDbiP56677.
PeptideAtlasiP56677.
PRIDEiP56677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034478; ENSMUSP00000034478; ENSMUSG00000031995.
GeneIDi19143.
KEGGimmu:19143.
UCSCiuc009ori.1. mouse.

Organism-specific databases

CTDi6768.
MGIiMGI:1338881. St14.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000136851.
HOVERGENiHBG012556.
InParanoidiP56677.
KOiK08670.
OMAiMMCVGFL.
OrthoDBiEOG091G0DF7.
PhylomeDBiP56677.
TreeFamiTF330647.

Enzyme and pathway databases

ReactomeiR-MMU-6809371. Formation of the cornified envelope.

Miscellaneous databases

PROiP56677.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031995.
ExpressionAtlasiP56677. baseline and differential.
GenevisibleiP56677. MM.

Family and domain databases

CDDicd00041. CUB. 2 hits.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
4.10.400.10. 4 hits.
InterProiIPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR017051. Peptidase_S1A_matripase.
IPR000082. SEA_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 4 hits.
PF01390. SEA. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF036370. ST14. 1 hit.
PRINTSiPR00261. LDLRECEPTOR.
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 4 hits.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57424. SSF57424. 4 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 3 hits.
PS50068. LDLRA_2. 4 hits.
PS50024. SEA. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiST14_MOUSE
AccessioniPrimary (citable) accession number: P56677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 4, 2001
Last modified: November 30, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.