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Protein

Mu/omega-theraphotoxin-Hs1a

Gene
N/A
Organism
Haplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lethal neurotoxin that blocks neuromuscular transmission by binding to the nicotinic acetylcholine receptor. Possess presynaptic activity that affects the release of neurotransmitters from autonomic nerve endings of both cholinergic and adrenergic neuroeffector junctions. Selectively inhibits N-type calcium channel (Cav2.2/CACNA1B) and has only very weak effect on L-type calcium channel (Cav1/CACNA1).2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Protein family/group databases

TCDBi8.B.3.1.1. the huwentoxin-1 (huwentoxin-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mu/omega-theraphotoxin-Hs1a
Short name:
Mu/omega-TRTX-Hs1a
Alternative name(s):
Huwentoxin-1
Short name:
HwTx-1
Huwentoxin-I
Short name:
HwTx-I
OrganismiHaplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Taxonomic identifieri29017 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000327. mu/omega-theraphotoxin-Hs1a.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.7 mg/kg by intraperitoneal injection, and 9.40 µg/kg by intracisternal injection into mice.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49A → Y: No decrease in bioactivity. 1 Publication1
Mutagenesisi51K → A: Decrease in bioactivity. 1 Publication1
Mutagenesisi68R → A: 92% decrease in bioactivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000003555822 – 481 PublicationAdd BLAST27
ChainiPRO_000003555949 – 81Mu/omega-theraphotoxin-Hs1aAdd BLAST33

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 651 Publication
Disulfide bondi57 ↔ 701 Publication
Disulfide bondi64 ↔ 771 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni59 – 62Combined sources4
Beta strandi66 – 70Combined sources5
Beta strandi72 – 74Combined sources3
Beta strandi76 – 79Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QK6NMR-A49-81[»]
ProteinModelPortaliP56676.
SMRiP56676.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56676.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. HwTx-I subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRASMFLALA GLVLLFVVCY ASESEEKEFP RELLFKFFAV DDFKGEERAC
60 70 80
KGVFDACTPG KNECCPNRVC SDKHKWCKWK L
Length:81
Mass (Da):9,319
Last modified:June 27, 2003 - v2
Checksum:iAB45FB6FEE7C8BF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263711 mRNA. Translation: AAP33078.1.
AF157504 mRNA. Translation: AAF25774.1.
PIRiA37479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263711 mRNA. Translation: AAP33078.1.
AF157504 mRNA. Translation: AAF25774.1.
PIRiA37479.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QK6NMR-A49-81[»]
ProteinModelPortaliP56676.
SMRiP56676.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.3.1.1. the huwentoxin-1 (huwentoxin-1) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000327. mu/omega-theraphotoxin-Hs1a.

Miscellaneous databases

EvolutionaryTraceiP56676.

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTXH1_HAPSC
AccessioniPrimary (citable) accession number: P56676
Secondary accession number(s): Q86C48, Q9NJC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 27, 2003
Last modified: November 2, 2016
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.