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P56649

- G3P_PANVR

UniProt

P56649 - G3P_PANVR

Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene
N/A
Organism
Panulirus versicolor (Spiny blue lobster) (Palinurus versicolor)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311NAD1 Publication
    Binding sitei76 – 761NAD; via carbonyl oxygenBy similarity
    Active sitei148 – 1481Nucleophile
    Sitei175 – 1751Activates thiol group during catalysis
    Binding sitei178 – 1781Glyceraldehyde 3-phosphateBy similarity
    Binding sitei230 – 2301Glyceraldehyde 3-phosphateBy similarity
    Binding sitei312 – 3121NAD1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 112NAD1 Publication

    GO - Molecular functioni

    1. glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro
    3. NADP binding Source: InterPro

    GO - Biological processi

    1. glucose metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    SABIO-RKP56649.
    UniPathwayiUPA00109; UER00184.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.12)
    Short name:
    GAPDH
    OrganismiPanulirus versicolor (Spiny blue lobster) (Palinurus versicolor)
    Taxonomic identifieri150436 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaCrustaceaMalacostracaEumalacostracaEucaridaDecapodaPleocyemataAchelataPalinuroideaPalinuridaePanulirus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 333333Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145506Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylserine

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP56649.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Structurei

    Secondary structure

    1
    333
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64
    Helixi10 – 2213
    Beta strandi26 – 305
    Beta strandi32 – 343
    Helixi36 – 449
    Turni47 – 493
    Beta strandi56 – 594
    Beta strandi62 – 654
    Beta strandi68 – 736
    Helixi78 – 803
    Helixi83 – 864
    Beta strandi90 – 934
    Beta strandi95 – 973
    Helixi101 – 1044
    Helixi105 – 1084
    Turni109 – 1113
    Beta strandi113 – 1197
    Beta strandi122 – 1243
    Turni129 – 1313
    Helixi133 – 1353
    Beta strandi142 – 1443
    Helixi148 – 16417
    Beta strandi166 – 17510
    Beta strandi181 – 1855
    Helixi193 – 1953
    Turni198 – 2003
    Beta strandi203 – 2053
    Helixi209 – 2168
    Helixi218 – 2203
    Turni221 – 2233
    Beta strandi224 – 2307
    Beta strandi237 – 24711
    Helixi251 – 26313
    Turni264 – 2696
    Beta strandi270 – 2734
    Helixi279 – 2824
    Beta strandi288 – 2925
    Turni293 – 2953
    Beta strandi297 – 3004
    Beta strandi303 – 3108
    Helixi314 – 33118

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CRWX-ray2.00G/R1-333[»]
    1DSSX-ray1.88G/R1-333[»]
    1IHXX-ray2.80A/B/C/D1-333[»]
    1IHYX-ray3.00A/B/C/D1-333[»]
    1SZJX-ray2.00G/R1-333[»]
    ProteinModelPortaliP56649.
    SMRiP56649. Positions 1-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56649.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni147 – 1493Glyceraldehyde 3-phosphate bindingBy similarity
    Regioni207 – 2082Glyceraldehyde 3-phosphate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10836. PTHR10836. 1 hit.
    PfamiPF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000149. GAP_DH. 1 hit.
    PRINTSiPR00078. G3PDHDRGNASE.
    SMARTiSM00846. Gp_dh_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
    PROSITEiPS00071. GAPDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56649-1 [UniParc]FASTAAdd to Basket

    « Hide

    SKIGINGFGR IGRLVLRAAL EMGAQVVAVN DPFIALEYMV YMFKYDSTHG    50
    MFKGEVKAED GALVVDGKKI TVFNEMKPEN IPWSKAGAEY IVESTGVFTT 100
    IEKASAHFKG GAKKVIISAP SADAPMFVCG VNLEKYSKDM KVVSNASCTT 150
    NCLAPVAKVL HENFEIVEGL MTTVHAVTAT QKTVDGPSAK DWRGGRGAAQ 200
    NIIPSSTGAA KAVGKVIPEL DGKLTGMAFR VPTPNVSVVD LTVRLGKECS 250
    YDDIKAAMKA ASEGPLQGVL GYTEDDVVSC DFTGDNRSSI FDAKAGIQLS 300
    KTFVKVVSWY DNEFGYSQRV IDLIKHMQKV DSA 333
    Length:333
    Mass (Da):35,724
    Last modified:July 15, 1999 - v1
    Checksum:i818572106E294C19
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CRW X-ray 2.00 G/R 1-333 [» ]
    1DSS X-ray 1.88 G/R 1-333 [» ]
    1IHX X-ray 2.80 A/B/C/D 1-333 [» ]
    1IHY X-ray 3.00 A/B/C/D 1-333 [» ]
    1SZJ X-ray 2.00 G/R 1-333 [» ]
    ProteinModelPortali P56649.
    SMRi P56649. Positions 1-333.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P56649.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00184 .
    SABIO-RK P56649.

    Miscellaneous databases

    EvolutionaryTracei P56649.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10836. PTHR10836. 1 hit.
    Pfami PF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000149. GAP_DH. 1 hit.
    PRINTSi PR00078. G3PDHDRGNASE.
    SMARTi SM00846. Gp_dh_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01534. GAPDH-I. 1 hit.
    PROSITEi PS00071. GAPDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of D-glyceraldehyde-3-phosphate dehydrogenase from Palinurus versicolor carrying the fluorescent NAD derivatives at 2.7-A resolution."
      Lin Z.J., Li J., Zhang F.M., Song S.Y., Yang J., Liang S.J., Tsou C.L.
      Arch. Biochem. Biophys. 302:161-166(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
    2. "Structure of holo-glyceraldehyde-3-phosphate dehydrogenase from Palinurus versicolor refined at 2-A resolution."
      Song S.Y., Li J., Lin Z.J.
      Acta Crystallogr. D 54:558-569(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) IN COMPLEX WITH NAD.
      Tissue: Muscle.

    Entry informationi

    Entry nameiG3P_PANVR
    AccessioniPrimary (citable) accession number: P56649
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3