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Protein

Alpha-conotoxin-like EpI

Gene
N/A
Organism
Conus episcopatus (Bishop's cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This peptide blocks mammalian nicotinic acetylcholine receptors composed of alpha-3/beta-2 and alpha-3/beta-4 subunits.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin-like EpI
OrganismiConus episcopatus (Bishop's cone)
Taxonomic identifieri88764 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri405. EpI.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Propeptidei17 – 39231 PublicationPRO_0000392692Add
BLAST
Peptidei40 – 5516Alpha-conotoxin-like EpIPRO_0000044457Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 47
Disulfide bondi42 ↔ 55
Modified residuei54 – 541Sulfotyrosine1 Publication
Modified residuei55 – 551Cysteine amide2 Publications

Keywords - PTMi

Amidation, Disulfide bond, Sulfation

Expressioni

Tissue specificityi

Expressed by the venom duct.

Structurei

Secondary structure

1
56
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi41 – 433Combined sources
Helixi45 – 506Combined sources
Turni52 – 543Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A0MX-ray1.10A/B40-55[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56638.

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR009958. Conotoxin_a-typ.
IPR018072. Conotoxin_a-typ_CS.
[Graphical view]
PfamiPF07365. Toxin_8. 1 hit.
[Graphical view]
PROSITEiPS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTVFLLVVL ATTVVSFTSD RASDSRKDAA SGLIALTIKG CCSDPRCNMN

NPDYCG
Length:56
Mass (Da):5,977
Last modified:March 23, 2010 - v2
Checksum:iB8523DFE3EF963CB
GO

Mass spectrometryi

Molecular mass is 1787.02 Da from positions 40 - 55. Determined by ESI. Without sulfation.1 Publication
Molecular mass is 1867.08 Da from positions 40 - 55. Determined by ESI. With sulfation.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BD261428 Unassigned DNA. No translation available.
PIRiA59042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BD261428 Unassigned DNA. No translation available.
PIRiA59042.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A0MX-ray1.10A/B40-55[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri405. EpI.

Miscellaneous databases

EvolutionaryTraceiP56638.

Family and domain databases

InterProiIPR009958. Conotoxin_a-typ.
IPR018072. Conotoxin_a-typ_CS.
[Graphical view]
PfamiPF07365. Toxin_8. 1 hit.
[Graphical view]
PROSITEiPS60014. ALPHA_CONOTOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE.
  2. "Alpha-conotoxin EpI, a novel sulfated peptide from Conus episcopatus that selectively targets neuronal nicotinic acetylcholine receptors."
    Loughnan M., Bond T., Atkins A., Cuevas J., Adams D.J., Broxton N.M., Livett B.G., Down J.G., Jones A., Alewood P.F., Lewis R.J.
    J. Biol. Chem. 273:15667-15674(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 40-55, SYNTHESIS OF 40-55, DISULFIDE BONDS, SULFATION AT TYR-54, AMIDATION AT CYS-55, MASS SPECTROMETRY.
  3. "The 1.1-A resolution crystal structure of [Tyr15]EpI, a novel alpha-conotoxin from Conus episcopatus, solved by direct methods."
    Hu S.H., Loughnan M., Miller R., Weeks C.M., Blessing R.H., Alewood P.F., Lewis R.J., Martin J.L.
    Biochemistry 37:11425-11433(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS) OF 40-55, AMIDATION AT CYS-55, DISULFIDE BONDS.

Entry informationi

Entry nameiCA1_CONEP
AccessioniPrimary (citable) accession number: P56638
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 23, 2010
Last modified: July 22, 2015
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.