P56635 (IF5A_PYRAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor 5A

Alternative name(s):
Hypusine-containing protein
eIF-5A

Gene names

Name:	eif5a
Ordered Locus Names:	PAE3337

Organism

Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827)

Taxonomic identifier

178306 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions by promoting the formation of the first peptide bond. HAMAP MF_00085
Subcellular location	Cytoplasm HAMAP MF_00085.
Sequence similarities	Belongs to the eIF-5A family.
Sequence caution	The sequence AAL64850.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
PTM	Hypusine
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: InterPro positive regulation of translational elongation Inferred from electronic annotation. Source: InterPro positive regulation of translational termination Inferred from electronic annotation. Source: InterPro translational frameshifting Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ribosome binding Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 138

138

Translation initiation factor 5A HAMAP MF_00085

PRO_0000142499

Amino acid modifications

Modified residue

42

1

Hypusine HAMAP MF_00085

Secondary structure

1

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138

Helix Strand Turn

Details...

Beta strand

10 – 13

4

Helix

14 – 16

3

Beta strand

22 – 25

4

Beta strand

28 – 38

11

Beta strand

47 – 54

8

Turn

55 – 57

3

Beta strand

60 – 66

7

Beta strand

69 – 73

5

Beta strand

77 – 95

19

Turn

96 – 98

3

Beta strand

101 – 105

5

Helix

106 – 108

3

Helix

111 – 114

4

Beta strand

122 – 128

7

Beta strand

131 – 137

7

Sequences

	Sequence		Length	Mass (Da)
	P56635 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: E44997F8AE912E9A	FASTA	138	15,411
	10 20 30 40 50 60 MVLKWVMSTK YVEAGELKEG SYVVIDGEPC RVVEIEKSKT GKHGSAKARI VAVGVFDGGK 70 80 90 100 110 120 RTLSLPVDAQ VEVPIIEKFT AQILSVSGDV IQLMDMRDYK TIEVPMKYVE EEAKGRLAPG 130 AEVEVWQILD RYKIIRVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum." Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I., Miller J.H. Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002) [PubMed: 11792869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827.
[2]	"Structure of translation initiation factor 5A from Pyrobaculum aerophilum at 1.75-A resolution." Peat T.S., Newman J., Waldo G.S., Berendzen J., Terwilliger T.C. Structure 6:1207-1214(1998) [PubMed: 9753699] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), HYPUSINE AT LYS-42.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE009441 Genomic DNA. Translation: AAL64850.1. Different initiation.

RefSeq

NP_560668.1. NC_003364.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BKB	X-ray	1.75	A	4-138	[»]

ProteinModelPortal

P56635.

SMR

P56635. Positions 4-138.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1464040.

GenomeReviews

Gene locus PAE3337 in contig AE009441_GR.

KEGG

pai:PAE3337.

NMPDR

fig|178306.1.peg.2358.

Phylogenomic databases

HOGENOM

HBG497107.

OMA

PTSSKVE.

ProtClustDB

PRK03999.

Enzyme and pathway databases

BioCyc

PAER178306:PAE3337-MONOMER.

Family and domain databases

HAMAP

MF_00085. eIF-5A.
[Tree]

InterPro

IPR005824. KOW.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR019769. Trans_elong_IF5A_hypusine_site.
IPR001884. Transl_elong_IF5A.
IPR022847. Transl_elong_IF5A_arc.
IPR020189. Transl_elong_IF5A_C.
IPR014722. Transl_SH3-like_sub.
IPR008991. Translation_prot_SH3-like.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.

KO

K03263.

PANTHER

PTHR11673. EIF5A_hypusine. 1 hit.
PTHR11673:SF1. PTHR11673:SF1. 1 hit.

Pfam

PF01287. eIF-5a. 1 hit.
PF00467. KOW. 1 hit.
[Graphical view]

PIRSF

PIRSF003025. eIF5A. 1 hit.

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.
SSF50104. Transl_SH3_like. 1 hit.

TIGRFAMs

TIGR00037. EIF_5A. 1 hit.

PROSITE

PS00302. IF5A_HYPUSINE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IF5A_PYRAE

Accession

Primary (citable) accession number: P56635

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 31, 2002
Last modified:	December 14, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program