Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aquaporin-9

Gene

Aqp9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na+, K+, Cl-, and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol.

GO - Molecular functioni

GO - Biological processi

  • amine transport Source: Ensembl
  • canalicular bile acid transport Source: RGD
  • cellular response to cAMP Source: Ensembl
  • response to mercury ion Source: Ensembl
  • water transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-RNO-432030. Transport of glycerol from adipocytes to the liver by Aquaporins.
R-RNO-432047. Passive transport by Aquaporins.

Protein family/group databases

TCDBi1.A.8.9.2. the major intrinsic protein (mip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-9
Short name:
AQP-9
Alternative name(s):
Aquaglyceroporin-9
Gene namesi
Name:Aqp9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi68433. Aqp9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929CytoplasmicSequence analysisAdd
BLAST
Transmembranei30 – 5021HelicalSequence analysisAdd
BLAST
Topological domaini51 – 544ExtracellularSequence analysis
Transmembranei55 – 7521HelicalSequence analysisAdd
BLAST
Topological domaini76 – 11035CytoplasmicSequence analysisAdd
BLAST
Transmembranei111 – 13121HelicalSequence analysisAdd
BLAST
Topological domaini132 – 15827ExtracellularSequence analysisAdd
BLAST
Transmembranei159 – 17921HelicalSequence analysisAdd
BLAST
Topological domaini180 – 18910CytoplasmicSequence analysis
Transmembranei190 – 21021HelicalSequence analysisAdd
BLAST
Topological domaini211 – 24535ExtracellularSequence analysisAdd
BLAST
Transmembranei246 – 26621HelicalSequence analysisAdd
BLAST
Topological domaini267 – 29529CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Aquaporin-9PRO_0000063966Add
BLAST

Proteomic databases

PaxDbiP56627.
PRIDEiP56627.

PTM databases

PhosphoSiteiP56627.

Expressioni

Gene expression databases

GenevisibleiP56627. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021442.

Structurei

3D structure databases

ProteinModelPortaliP56627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi84 – 863NPA 1
Motifi216 – 2183NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0224. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00510000046311.
HOGENOMiHOG000288287.
HOVERGENiHBG106057.
InParanoidiP56627.
KOiK09877.
OMAiIITINTG.
OrthoDBiEOG7J9VPP.
PhylomeDBiP56627.
TreeFamiTF313173.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR015685. Aquaporin_9.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02021. AQUAPORIN9.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSEKDGAKK SLMQRLALKS RIAKETLSEF LGTFIMIVLG CSSIAQAVLS
60 70 80 90 100
RERFGGIITI NIGFASAVVM ALYVTFGISG GHINPAVSFA MCAFGRMEWF
110 120 130 140 150
KFPFYVGAQF LGAFVGAATV FGIYYDGLMA FAGGKLLVVG ENATAFIFAT
160 170 180 190 200
YPAPFISTPG AFVDQVVSTM FLLLIVFAMF DSRNLGVPRG LEPVVIGLLI
210 220 230 240 250
IVLSCSLGLN SGCAMNPARD LSPRLFTALA GWGFEVFTVG NNFWWIPVVG
260 270 280 290
PMIGAFLGGL IYILFIQMHH SKLDPDMKAE PSENNLEKHE LSVIM
Length:295
Mass (Da):31,871
Last modified:December 15, 1998 - v1
Checksum:i09FD8B1DE936E61F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931A → V in BAA33680 (PubMed:10205677).Curated
Sequence conflicti197 – 1971G → S in BAA33680 (PubMed:10205677).Curated
Sequence conflicti207 – 2071L → P in BAA33680 (PubMed:10205677).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016406 mRNA. Translation: AAC36020.1.
AB013112 mRNA. Translation: BAA33680.1.
BC085731 mRNA. Translation: AAH85731.1.
RefSeqiNP_075249.1. NM_022960.2.
XP_006243424.1. XM_006243362.1.
XP_008764558.1. XM_008766336.1.
UniGeneiRn.30018.

Genome annotation databases

EnsembliENSRNOT00000087980; ENSRNOP00000069377; ENSRNOG00000061883.
GeneIDi65054.
KEGGirno:65054.
UCSCiRGD:68433. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016406 mRNA. Translation: AAC36020.1.
AB013112 mRNA. Translation: BAA33680.1.
BC085731 mRNA. Translation: AAH85731.1.
RefSeqiNP_075249.1. NM_022960.2.
XP_006243424.1. XM_006243362.1.
XP_008764558.1. XM_008766336.1.
UniGeneiRn.30018.

3D structure databases

ProteinModelPortaliP56627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021442.

Protein family/group databases

TCDBi1.A.8.9.2. the major intrinsic protein (mip) family.

PTM databases

PhosphoSiteiP56627.

Proteomic databases

PaxDbiP56627.
PRIDEiP56627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000087980; ENSRNOP00000069377; ENSRNOG00000061883.
GeneIDi65054.
KEGGirno:65054.
UCSCiRGD:68433. rat.

Organism-specific databases

CTDi366.
RGDi68433. Aqp9.

Phylogenomic databases

eggNOGiKOG0224. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00510000046311.
HOGENOMiHOG000288287.
HOVERGENiHBG106057.
InParanoidiP56627.
KOiK09877.
OMAiIITINTG.
OrthoDBiEOG7J9VPP.
PhylomeDBiP56627.
TreeFamiTF313173.

Enzyme and pathway databases

ReactomeiR-RNO-432030. Transport of glycerol from adipocytes to the liver by Aquaporins.
R-RNO-432047. Passive transport by Aquaporins.

Miscellaneous databases

NextBioi613911.
PROiP56627.

Gene expression databases

GenevisibleiP56627. RN.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR015685. Aquaporin_9.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02021. AQUAPORIN9.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver."
    Ko S.B., Uchida S., Naruse S., Kuwahara M., Ishibashi K., Marumo F., Hayakawa T., Sasaki S.
    Biochem. Mol. Biol. Int. 47:309-318(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiAQP9_RAT
AccessioniPrimary (citable) accession number: P56627
Secondary accession number(s): O88815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.