Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protoporphyrinogen oxidase

Gene

PPOX

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase (PPOX)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42FAD; via amide nitrogenBy similarity1
Binding sitei257FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei449FAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14FADBy similarity6
Nucleotide bindingi34 – 35FADBy similarity2
Nucleotide bindingi57 – 60FADBy similarity4
Nucleotide bindingi454 – 456FADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-BTA-189451 Heme biosynthesis
UniPathwayiUPA00251; UER00324

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:PPOX
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Organism-specific databases

VGNCiVGNC:33219 PPOX

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352691 – 477Protoporphyrinogen oxidaseAdd BLAST477

Proteomic databases

PaxDbiP56602
PRIDEiP56602

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Gene expression databases

BgeeiENSBTAG00000021894

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000053328

Structurei

3D structure databases

ProteinModelPortaliP56602
SMRiP56602
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiKOG1276 Eukaryota
COG1232 LUCA
GeneTreeiENSGT00390000008744
KOiK00231
OMAiPMICGIC
OrthoDBiEOG091G08PW

Family and domain databases

Gene3Di3.50.50.60, 1 hit
InterProiView protein in InterPro
IPR002937 Amino_oxidase
IPR036188 FAD/NAD-bd_sf
IPR004572 Protoporphyrinogen_oxidase
PfamiView protein in Pfam
PF01593 Amino_oxidase, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
TIGRFAMsiTIGR00562 proto_IX_ox, 1 hit

Sequencei

Sequence statusi: Complete.

P56602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRTVVVLGG GISGLAASYY LSRAPCPPKV VLVEGSERLG GWIRSVRGPD
60 70 80 90 100
GAIFELGPRG IRPAGALGAR TLLLVSELGL DSEVLPVRGD HPAAQNRFLY
110 120 130 140 150
VGGALHALPS GIRGLLRPSP PFSKPLFWAG LRDLTTPRGK DPDETVHSFA
160 170 180 190 200
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG
210 220 230 240 250
LLLGAGRGPQ LDSALIRQAQ AERWSQWSLR GGLETLPQAL HAHLTSRGVS
260 270 280 290 300
VLQGQPVCGL SLQAEGRWKV SLEDSSLEAD HIISAIPASV LSKLLPAEAT
310 320 330 340 350
PLARALSTIT AVSVAVVNLQ YRGARLPVQG FGHLVPSSED PVILGIVYDS
360 370 380 390 400
VAFPEQDGSL PGLRLTVMLG GSWLQTLEAR GCVLSQELLQ QEAEKAAATQ
410 420 430 440 450
LGLNEPPSHC LVHLHKNSIP QYTLGHWQKL ESAAQFLAAQ KLPLTLAGAS
460 470
YEGVAVNDCI ESGRQAAARV LGTEPNS
Length:477
Mass (Da):50,612
Last modified:February 19, 2014 - v2
Checksum:iE2A4581893001BEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02006959 Genomic DNA No translation available.
PIRiS65629
RefSeqiNP_001179355.1, NM_001192426.1
XP_005203553.1, XM_005203496.2
XP_005203554.1, XM_005203497.3
UniGeneiBt.4335

Genome annotation databases

EnsembliENSBTAT00000061124; ENSBTAP00000053328; ENSBTAG00000021894
GeneIDi515770
KEGGibta:515770

Similar proteinsi

Entry informationi

Entry nameiPPOX_BOVIN
AccessioniPrimary (citable) accession number: P56602
Secondary accession number(s): E1BPX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 19, 2014
Last modified: March 28, 2018
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health