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Protein

Protoporphyrinogen oxidase

Gene

PPOX

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathway:iprotoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase (PPOX)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421FAD; via amide nitrogenBy similarity
Binding sitei257 – 2571FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei449 – 4491FAD; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADBy similarity
Nucleotide bindingi34 – 352FADBy similarity
Nucleotide bindingi57 – 604FADBy similarity
Nucleotide bindingi454 – 4563FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_299026. Heme biosynthesis.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:PPOX
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Protoporphyrinogen oxidasePRO_0000135269Add
BLAST

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000053328.

Structurei

3D structure databases

ProteinModelPortaliP56602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiCOG1232.
GeneTreeiENSGT00390000008744.
KOiK00231.
OMAiCEWGPNG.
OrthoDBiEOG7DJSMB.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P56602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRTVVVLGG GISGLAASYY LSRAPCPPKV VLVEGSERLG GWIRSVRGPD
60 70 80 90 100
GAIFELGPRG IRPAGALGAR TLLLVSELGL DSEVLPVRGD HPAAQNRFLY
110 120 130 140 150
VGGALHALPS GIRGLLRPSP PFSKPLFWAG LRDLTTPRGK DPDETVHSFA
160 170 180 190 200
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG
210 220 230 240 250
LLLGAGRGPQ LDSALIRQAQ AERWSQWSLR GGLETLPQAL HAHLTSRGVS
260 270 280 290 300
VLQGQPVCGL SLQAEGRWKV SLEDSSLEAD HIISAIPASV LSKLLPAEAT
310 320 330 340 350
PLARALSTIT AVSVAVVNLQ YRGARLPVQG FGHLVPSSED PVILGIVYDS
360 370 380 390 400
VAFPEQDGSL PGLRLTVMLG GSWLQTLEAR GCVLSQELLQ QEAEKAAATQ
410 420 430 440 450
LGLNEPPSHC LVHLHKNSIP QYTLGHWQKL ESAAQFLAAQ KLPLTLAGAS
460 470
YEGVAVNDCI ESGRQAAARV LGTEPNS
Length:477
Mass (Da):50,612
Last modified:February 19, 2014 - v2
Checksum:iE2A4581893001BEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02006959 Genomic DNA. No translation available.
PIRiS65629.
RefSeqiNP_001179355.1. NM_001192426.1.
XP_005203553.1. XM_005203496.1.
XP_005203554.1. XM_005203497.2.
UniGeneiBt.4335.

Genome annotation databases

EnsembliENSBTAT00000061124; ENSBTAP00000053328; ENSBTAG00000021894.
GeneIDi515770.
KEGGibta:515770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02006959 Genomic DNA. No translation available.
PIRiS65629.
RefSeqiNP_001179355.1. NM_001192426.1.
XP_005203553.1. XM_005203496.1.
XP_005203554.1. XM_005203497.2.
UniGeneiBt.4335.

3D structure databases

ProteinModelPortaliP56602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000053328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000061124; ENSBTAP00000053328; ENSBTAG00000021894.
GeneIDi515770.
KEGGibta:515770.

Organism-specific databases

CTDi5498.

Phylogenomic databases

eggNOGiCOG1232.
GeneTreeiENSGT00390000008744.
KOiK00231.
OMAiCEWGPNG.
OrthoDBiEOG7DJSMB.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
ReactomeiREACT_299026. Heme biosynthesis.

Miscellaneous databases

NextBioi20871996.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. "Induction of terminal enzymes for heme biosynthesis during differentiation of mouse erythroleukemia cells."
    Taketani S., Yoshinaga T., Furukawa T., Kohno H., Tokunaga R., Nishimura K., Inokuchi H.
    Eur. J. Biochem. 230:760-765(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-14 AND 164-178, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Liver.

Entry informationi

Entry nameiPPOX_BOVIN
AccessioniPrimary (citable) accession number: P56602
Secondary accession number(s): E1BPX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 19, 2014
Last modified: July 22, 2015
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.