Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P56601 (PPOX_MYXXA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protoporphyrinogen oxidase

Short name=PPO
EC=1.3.3.4
Gene names
Name:hemY
OrganismMyxococcus xanthus
Taxonomic identifier34 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Ref.3

Catalytic activity

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2. Ref.3

Cofactor

Binds 1 FAD per subunit. Ref.3

Enzyme regulation

Strongly inhibited by acifluorfen. Ref.3

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.

Subunit structure

Monomer. Homodimer. Ref.3 Ref.4

Subcellular location

Cytoplasm. Membrane Ref.4.

Sequence similarities

Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   Cellular componentCytoplasm
Membrane
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionoxygen-dependent protoporphyrinogen oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 471471Protoporphyrinogen oxidase
PRO_0000135267

Regions

Nucleotide binding16 – 216FAD
Nucleotide binding39 – 402FAD
Nucleotide binding61 – 644FAD
Nucleotide binding446 – 4483FAD

Sites

Binding site471FAD; via amide nitrogen
Binding site2511FAD; via amide nitrogen and carbonyl oxygen
Binding site4081FAD By similarity

Secondary structure

........................................................................................ 471
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56601 [UniParc].

Last modified December 15, 1998. Version 1.
Checksum: 2E9909D464F81515

FASTA47149,388
        10         20         30         40         50         60 
MHHMPRTTGM NVAVVGGGIS GLAVAHHLRS RGTDAVLLES SARLGGAVGT HALAGYLVEQ 

        70         80         90        100        110        120 
GPNSFLDREP ATRALAAALN LEGRIRAADP AAKRRYVYTR GRLRSVPASP PAFLASDILP 

       130        140        150        160        170        180 
LGARLRVAGE LFSRRAPEGV DESLAAFGRR HLGHRATQVL LDAVQTGIYA GDVEQLSVAA 

       190        200        210        220        230        240 
TFPMLVKMER EHRSLILGAI RAQKAQRQAA LPAGTAPKLS GALSTFDGGL QVLIDALAAS 

       250        260        270        280        290        300 
LGDAAHVGAR VEGLAREDGG WRLIIEEHGR RAELSVAQVV LAAPAHATAK LLRPLDDALA 

       310        320        330        340        350        360 
ALVAGIAYAP IAVVHLGFDA GTLPAPDGFG FLVPAEEQRR MLGAIHASTT FPFRAEGGRV 

       370        380        390        400        410        420 
LYSCMVGGAR QPGLVEQDED ALAALAREEL KALAGVTARP SFTRVFRWPL GIPQYNLGHL 

       430        440        450        460        470 
ERVAAIDAAL QRLPGLHLIG NAYKGVGLND CIRNAAQLAD ALVAGNTSHA P 

« Hide

References

[1]"csgA expression entrains Myxococcus xanthus development."
Li S., Lee B.U., Shimkets L.J.
Genes Dev. 6:401-410(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Shimkets L.J.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]"Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme."
Dailey H.A., Dailey T.A.
J. Biol. Chem. 271:8714-8718(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, FUNCTION, COFACTOR, ENZYME REGULATION, SUBUNIT.
[4]"Crystal structure of protoporphyrinogen oxidase from Myxococcus xanthus and its complex with the inhibitor acifluorfen."
Corradi H.R., Corrigall A.V., Boix E., Mohan C.G., Sturrock E.D., Meissner P.N., Acharya K.R.
J. Biol. Chem. 281:38625-38633(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 4-471 IN COMPLEX WITH FAD AND ACIFLUORFEN, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L27429 Genomic DNA. Translation: AAM28643.1.
AF098938 Genomic DNA. Translation: AAD13609.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IVDX-ray2.30A/B4-471[»]
2IVEX-ray2.70A/B4-471[»]
ProteinModelPortalP56601.
SMRP56601. Positions 10-464.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA1.3.3.4. 3551.
SABIO-RKP56601.
UniPathwayUPA00251; UER00324.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR004572. Protoporphyrinogen_oxidase.
IPR027418. Protoporphyrinogen_oxidase_C.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00562. proto_IX_ox. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP56601.

Entry information

Entry namePPOX_MYXXA
AccessionPrimary (citable) accession number: P56601
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways