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P56601

- PPOX_MYXXA

UniProt

P56601 - PPOX_MYXXA

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Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Myxococcus xanthus
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

Binds 1 FAD per subunit.1 Publication

Enzyme regulationi

Strongly inhibited by acifluorfen.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 471FAD; via amide nitrogen1 Publication
Binding sitei251 – 2511FAD; via amide nitrogen and carbonyl oxygen1 Publication
Binding sitei408 – 4081FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 216FAD1 Publication
Nucleotide bindingi39 – 402FAD1 Publication
Nucleotide bindingi61 – 644FAD1 Publication
Nucleotide bindingi446 – 4483FAD1 Publication

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.3.3.4. 3551.
SABIO-RKP56601.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:hemY
OrganismiMyxococcus xanthus
Taxonomic identifieri34 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

Subcellular locationi

Cytoplasm 1 Publication. Membrane 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Protoporphyrinogen oxidasePRO_0000135267Add
BLAST

Interactioni

Subunit structurei

Monomer. Homodimer.2 Publications

Structurei

Secondary structure

1
471
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 154Combined sources
Helixi19 – 2911Combined sources
Turni30 – 323Combined sources
Beta strandi35 – 384Combined sources
Beta strandi40 – 456Combined sources
Beta strandi50 – 534Combined sources
Beta strandi56 – 616Combined sources
Helixi70 – 789Combined sources
Helixi82 – 843Combined sources
Beta strandi85 – 873Combined sources
Beta strandi95 – 995Combined sources
Beta strandi102 – 1054Combined sources
Helixi110 – 1145Combined sources
Beta strandi117 – 1193Combined sources
Helixi121 – 1288Combined sources
Helixi129 – 1324Combined sources
Helixi144 – 1518Combined sources
Helixi154 – 1596Combined sources
Helixi161 – 1699Combined sources
Turni173 – 1753Combined sources
Helixi178 – 1814Combined sources
Helixi183 – 19210Combined sources
Helixi195 – 20612Combined sources
Beta strandi223 – 2264Combined sources
Helixi231 – 24111Combined sources
Helixi242 – 2443Combined sources
Beta strandi245 – 25511Combined sources
Beta strandi262 – 2676Combined sources
Beta strandi270 – 2756Combined sources
Beta strandi277 – 2815Combined sources
Helixi285 – 2928Combined sources
Turni293 – 2953Combined sources
Helixi297 – 3048Combined sources
Beta strandi311 – 3188Combined sources
Beta strandi328 – 3325Combined sources
Helixi335 – 3373Combined sources
Beta strandi343 – 3464Combined sources
Helixi347 – 3504Combined sources
Helixi352 – 3543Combined sources
Beta strandi360 – 3678Combined sources
Helixi372 – 3765Combined sources
Helixi379 – 39416Combined sources
Beta strandi401 – 41010Combined sources
Helixi419 – 43113Combined sources
Beta strandi436 – 4383Combined sources
Turni441 – 4433Combined sources
Helixi448 – 46215Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IVDX-ray2.30A/B4-471[»]
2IVEX-ray2.70A/B4-471[»]
ProteinModelPortaliP56601.
SMRiP56601. Positions 10-464.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56601.

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P56601 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHHMPRTTGM NVAVVGGGIS GLAVAHHLRS RGTDAVLLES SARLGGAVGT
60 70 80 90 100
HALAGYLVEQ GPNSFLDREP ATRALAAALN LEGRIRAADP AAKRRYVYTR
110 120 130 140 150
GRLRSVPASP PAFLASDILP LGARLRVAGE LFSRRAPEGV DESLAAFGRR
160 170 180 190 200
HLGHRATQVL LDAVQTGIYA GDVEQLSVAA TFPMLVKMER EHRSLILGAI
210 220 230 240 250
RAQKAQRQAA LPAGTAPKLS GALSTFDGGL QVLIDALAAS LGDAAHVGAR
260 270 280 290 300
VEGLAREDGG WRLIIEEHGR RAELSVAQVV LAAPAHATAK LLRPLDDALA
310 320 330 340 350
ALVAGIAYAP IAVVHLGFDA GTLPAPDGFG FLVPAEEQRR MLGAIHASTT
360 370 380 390 400
FPFRAEGGRV LYSCMVGGAR QPGLVEQDED ALAALAREEL KALAGVTARP
410 420 430 440 450
SFTRVFRWPL GIPQYNLGHL ERVAAIDAAL QRLPGLHLIG NAYKGVGLND
460 470
CIRNAAQLAD ALVAGNTSHA P
Length:471
Mass (Da):49,388
Last modified:December 15, 1998 - v1
Checksum:i2E9909D464F81515
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27429 Genomic DNA. Translation: AAM28643.1.
AF098938 Genomic DNA. Translation: AAD13609.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27429 Genomic DNA. Translation: AAM28643.1 .
AF098938 Genomic DNA. Translation: AAD13609.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IVD X-ray 2.30 A/B 4-471 [» ]
2IVE X-ray 2.70 A/B 4-471 [» ]
ProteinModelPortali P56601.
SMRi P56601. Positions 10-464.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
BRENDAi 1.3.3.4. 3551.
SABIO-RK P56601.

Miscellaneous databases

EvolutionaryTracei P56601.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "csgA expression entrains Myxococcus xanthus development."
    Li S., Lee B.U., Shimkets L.J.
    Genes Dev. 6:401-410(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Shimkets L.J.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. "Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme."
    Dailey H.A., Dailey T.A.
    J. Biol. Chem. 271:8714-8718(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, COFACTOR, ENZYME REGULATION, SUBUNIT.
  4. "Crystal structure of protoporphyrinogen oxidase from Myxococcus xanthus and its complex with the inhibitor acifluorfen."
    Corradi H.R., Corrigall A.V., Boix E., Mohan C.G., Sturrock E.D., Meissner P.N., Acharya K.R.
    J. Biol. Chem. 281:38625-38633(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 4-471 IN COMPLEX WITH FAD AND ACIFLUORFEN, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPPOX_MYXXA
AccessioniPrimary (citable) accession number: P56601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3