P56601 (PPOX_MYXXA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoporphyrinogen oxidase
Short name=PPO
EC=1.3.3.4

Gene names

Name:

hemY

Organism

Myxococcus xanthus

Taxonomic identifier

34 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. May have an essential extracellular function during development, possibly as a pheromone.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	Binds 1 FAD per dimer.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxygen-dependent protoporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 471

471

Protoporphyrinogen oxidase

PRO_0000135267

Regions

Nucleotide binding

16 – 21

6

FAD Potential

Secondary structure

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471

Helix Strand Turn

Details...

Beta strand

12 – 15

4

Helix

19 – 29

11

Turn

30 – 32

3

Beta strand

35 – 38

4

Beta strand

40 – 45

6

Beta strand

50 – 53

4

Beta strand

56 – 61

6

Helix

70 – 78

9

Helix

82 – 84

3

Beta strand

95 – 99

5

Beta strand

102 – 105

4

Helix

110 – 114

5

Beta strand

117 – 119

3

Helix

121 – 128

8

Helix

129 – 132

4

Helix

144 – 151

8

Helix

154 – 159

6

Helix

161 – 169

9

Turn

173 – 175

3

Helix

178 – 181

4

Helix

183 – 192

10

Helix

195 – 206

12

Beta strand

223 – 226

4

Helix

231 – 241

11

Helix

242 – 244

3

Beta strand

245 – 255

11

Beta strand

262 – 267

6

Beta strand

270 – 275

6

Beta strand

277 – 281

5

Helix

285 – 292

8

Turn

293 – 295

3

Helix

297 – 304

8

Beta strand

311 – 318

8

Beta strand

328 – 332

5

Helix

335 – 337

3

Beta strand

343 – 346

4

Helix

347 – 350

4

Helix

352 – 354

3

Beta strand

360 – 367

8

Helix

372 – 376

5

Helix

379 – 394

16

Beta strand

401 – 410

10

Helix

419 – 431

13

Beta strand

436 – 438

3

Turn

441 – 443

3

Helix

448 – 462

15

Sequences

	Sequence		Length	Mass (Da)
	P56601 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 2E9909D464F81515	FASTA	471	49,388
	10 20 30 40 50 60 MHHMPRTTGM NVAVVGGGIS GLAVAHHLRS RGTDAVLLES SARLGGAVGT HALAGYLVEQ 70 80 90 100 110 120 GPNSFLDREP ATRALAAALN LEGRIRAADP AAKRRYVYTR GRLRSVPASP PAFLASDILP 130 140 150 160 170 180 LGARLRVAGE LFSRRAPEGV DESLAAFGRR HLGHRATQVL LDAVQTGIYA GDVEQLSVAA 190 200 210 220 230 240 TFPMLVKMER EHRSLILGAI RAQKAQRQAA LPAGTAPKLS GALSTFDGGL QVLIDALAAS 250 260 270 280 290 300 LGDAAHVGAR VEGLAREDGG WRLIIEEHGR RAELSVAQVV LAAPAHATAK LLRPLDDALA 310 320 330 340 350 360 ALVAGIAYAP IAVVHLGFDA GTLPAPDGFG FLVPAEEQRR MLGAIHASTT FPFRAEGGRV 370 380 390 400 410 420 LYSCMVGGAR QPGLVEQDED ALAALAREEL KALAGVTARP SFTRVFRWPL GIPQYNLGHL 430 440 450 460 470 ERVAAIDAAL QRLPGLHLIG NAYKGVGLND CIRNAAQLAD ALVAGNTSHA P

References

[1]	"csgA expression entrains Myxococcus xanthus development." Li S., Lee B.U., Shimkets L.J. Genes Dev. 6:401-410(1992) [PubMed: 1372277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Shimkets L.J. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme." Dailey H.A., Dailey T.A. J. Biol. Chem. 271:8714-8718(1996) [PubMed: 8621504] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L27429 Genomic DNA. Translation: AAM28643.1.
AF098938 Genomic DNA. Translation: AAD13609.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IVD	X-ray	2.30	A/B	4-471	[»]
2IVE	X-ray	2.70	A/B	4-471	[»]

ProteinModelPortal

P56601.

SMR

P56601. Positions 10-464.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

1.3.3.4. 3551.

Family and domain databases

InterPro

IPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF01593. Amino_oxidase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00562. Proto_IX_ox. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PPOX_MYXXA

Accession

Primary (citable) accession number: P56601

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program