Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P56601

- PPOX_MYXXA

UniProt

P56601 - PPOX_MYXXA

Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Myxococcus xanthus
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (15 Dec 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

    Catalytic activityi

    Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

    Cofactori

    Binds 1 FAD per subunit.1 Publication

    Enzyme regulationi

    Strongly inhibited by acifluorfen.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei47 – 471FAD; via amide nitrogen1 Publication
    Binding sitei251 – 2511FAD; via amide nitrogen and carbonyl oxygen1 Publication
    Binding sitei408 – 4081FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 216FAD1 Publication
    Nucleotide bindingi39 – 402FAD1 Publication
    Nucleotide bindingi61 – 644FAD1 Publication
    Nucleotide bindingi446 – 4483FAD1 Publication

    GO - Molecular functioni

    1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BRENDAi1.3.3.4. 3551.
    SABIO-RKP56601.
    UniPathwayiUPA00251; UER00324.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protoporphyrinogen oxidase (EC:1.3.3.4)
    Short name:
    PPO
    Gene namesi
    Name:hemY
    OrganismiMyxococcus xanthus
    Taxonomic identifieri34 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

    Subcellular locationi

    Cytoplasm 1 Publication. Membrane 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 471471Protoporphyrinogen oxidasePRO_0000135267Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer. Homodimer.2 Publications

    Structurei

    Secondary structure

    1
    471
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi12 – 154
    Helixi19 – 2911
    Turni30 – 323
    Beta strandi35 – 384
    Beta strandi40 – 456
    Beta strandi50 – 534
    Beta strandi56 – 616
    Helixi70 – 789
    Helixi82 – 843
    Beta strandi85 – 873
    Beta strandi95 – 995
    Beta strandi102 – 1054
    Helixi110 – 1145
    Beta strandi117 – 1193
    Helixi121 – 1288
    Helixi129 – 1324
    Helixi144 – 1518
    Helixi154 – 1596
    Helixi161 – 1699
    Turni173 – 1753
    Helixi178 – 1814
    Helixi183 – 19210
    Helixi195 – 20612
    Beta strandi223 – 2264
    Helixi231 – 24111
    Helixi242 – 2443
    Beta strandi245 – 25511
    Beta strandi262 – 2676
    Beta strandi270 – 2756
    Beta strandi277 – 2815
    Helixi285 – 2928
    Turni293 – 2953
    Helixi297 – 3048
    Beta strandi311 – 3188
    Beta strandi328 – 3325
    Helixi335 – 3373
    Beta strandi343 – 3464
    Helixi347 – 3504
    Helixi352 – 3543
    Beta strandi360 – 3678
    Helixi372 – 3765
    Helixi379 – 39416
    Beta strandi401 – 41010
    Helixi419 – 43113
    Beta strandi436 – 4383
    Turni441 – 4433
    Helixi448 – 46215

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IVDX-ray2.30A/B4-471[»]
    2IVEX-ray2.70A/B4-471[»]
    ProteinModelPortaliP56601.
    SMRiP56601. Positions 10-464.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56601.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the protoporphyrinogen oxidase family.Curated

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.660.20. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P56601-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHHMPRTTGM NVAVVGGGIS GLAVAHHLRS RGTDAVLLES SARLGGAVGT    50
    HALAGYLVEQ GPNSFLDREP ATRALAAALN LEGRIRAADP AAKRRYVYTR 100
    GRLRSVPASP PAFLASDILP LGARLRVAGE LFSRRAPEGV DESLAAFGRR 150
    HLGHRATQVL LDAVQTGIYA GDVEQLSVAA TFPMLVKMER EHRSLILGAI 200
    RAQKAQRQAA LPAGTAPKLS GALSTFDGGL QVLIDALAAS LGDAAHVGAR 250
    VEGLAREDGG WRLIIEEHGR RAELSVAQVV LAAPAHATAK LLRPLDDALA 300
    ALVAGIAYAP IAVVHLGFDA GTLPAPDGFG FLVPAEEQRR MLGAIHASTT 350
    FPFRAEGGRV LYSCMVGGAR QPGLVEQDED ALAALAREEL KALAGVTARP 400
    SFTRVFRWPL GIPQYNLGHL ERVAAIDAAL QRLPGLHLIG NAYKGVGLND 450
    CIRNAAQLAD ALVAGNTSHA P 471
    Length:471
    Mass (Da):49,388
    Last modified:December 15, 1998 - v1
    Checksum:i2E9909D464F81515
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27429 Genomic DNA. Translation: AAM28643.1.
    AF098938 Genomic DNA. Translation: AAD13609.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27429 Genomic DNA. Translation: AAM28643.1 .
    AF098938 Genomic DNA. Translation: AAD13609.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IVD X-ray 2.30 A/B 4-471 [» ]
    2IVE X-ray 2.70 A/B 4-471 [» ]
    ProteinModelPortali P56601.
    SMRi P56601. Positions 10-464.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00324 .
    BRENDAi 1.3.3.4. 3551.
    SABIO-RK P56601.

    Miscellaneous databases

    EvolutionaryTracei P56601.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.660.20. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "csgA expression entrains Myxococcus xanthus development."
      Li S., Lee B.U., Shimkets L.J.
      Genes Dev. 6:401-410(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Shimkets L.J.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. "Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme."
      Dailey H.A., Dailey T.A.
      J. Biol. Chem. 271:8714-8718(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION, COFACTOR, ENZYME REGULATION, SUBUNIT.
    4. "Crystal structure of protoporphyrinogen oxidase from Myxococcus xanthus and its complex with the inhibitor acifluorfen."
      Corradi H.R., Corrigall A.V., Boix E., Mohan C.G., Sturrock E.D., Meissner P.N., Acharya K.R.
      J. Biol. Chem. 281:38625-38633(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 4-471 IN COMPLEX WITH FAD AND ACIFLUORFEN, SUBUNIT, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPPOX_MYXXA
    AccessioniPrimary (citable) accession number: P56601
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3