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P56601

- PPOX_MYXXA

UniProt

P56601 - PPOX_MYXXA

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Protein
Protoporphyrinogen oxidase
Gene
hemY
Organism
Myxococcus xanthus
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

Binds 1 FAD per subunit.1 Publication

Enzyme regulationi

Strongly inhibited by acifluorfen.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 471FAD; via amide nitrogen
Binding sitei251 – 2511FAD; via amide nitrogen and carbonyl oxygen
Binding sitei408 – 4081FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 216FAD
Nucleotide bindingi39 – 402FAD
Nucleotide bindingi61 – 644FAD
Nucleotide bindingi446 – 4483FAD

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.3.3.4. 3551.
SABIO-RKP56601.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:hemY
OrganismiMyxococcus xanthus
Taxonomic identifieri34 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus

Subcellular locationi

Cytoplasm. Membrane 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Protoporphyrinogen oxidase
PRO_0000135267Add
BLAST

Interactioni

Subunit structurei

Monomer. Homodimer.2 Publications

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 154
Helixi19 – 2911
Turni30 – 323
Beta strandi35 – 384
Beta strandi40 – 456
Beta strandi50 – 534
Beta strandi56 – 616
Helixi70 – 789
Helixi82 – 843
Beta strandi85 – 873
Beta strandi95 – 995
Beta strandi102 – 1054
Helixi110 – 1145
Beta strandi117 – 1193
Helixi121 – 1288
Helixi129 – 1324
Helixi144 – 1518
Helixi154 – 1596
Helixi161 – 1699
Turni173 – 1753
Helixi178 – 1814
Helixi183 – 19210
Helixi195 – 20612
Beta strandi223 – 2264
Helixi231 – 24111
Helixi242 – 2443
Beta strandi245 – 25511
Beta strandi262 – 2676
Beta strandi270 – 2756
Beta strandi277 – 2815
Helixi285 – 2928
Turni293 – 2953
Helixi297 – 3048
Beta strandi311 – 3188
Beta strandi328 – 3325
Helixi335 – 3373
Beta strandi343 – 3464
Helixi347 – 3504
Helixi352 – 3543
Beta strandi360 – 3678
Helixi372 – 3765
Helixi379 – 39416
Beta strandi401 – 41010
Helixi419 – 43113
Beta strandi436 – 4383
Turni441 – 4433
Helixi448 – 46215

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IVDX-ray2.30A/B4-471[»]
2IVEX-ray2.70A/B4-471[»]
ProteinModelPortaliP56601.
SMRiP56601. Positions 10-464.

Miscellaneous databases

EvolutionaryTraceiP56601.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P56601-1 [UniParc]FASTAAdd to Basket

« Hide

MHHMPRTTGM NVAVVGGGIS GLAVAHHLRS RGTDAVLLES SARLGGAVGT    50
HALAGYLVEQ GPNSFLDREP ATRALAAALN LEGRIRAADP AAKRRYVYTR 100
GRLRSVPASP PAFLASDILP LGARLRVAGE LFSRRAPEGV DESLAAFGRR 150
HLGHRATQVL LDAVQTGIYA GDVEQLSVAA TFPMLVKMER EHRSLILGAI 200
RAQKAQRQAA LPAGTAPKLS GALSTFDGGL QVLIDALAAS LGDAAHVGAR 250
VEGLAREDGG WRLIIEEHGR RAELSVAQVV LAAPAHATAK LLRPLDDALA 300
ALVAGIAYAP IAVVHLGFDA GTLPAPDGFG FLVPAEEQRR MLGAIHASTT 350
FPFRAEGGRV LYSCMVGGAR QPGLVEQDED ALAALAREEL KALAGVTARP 400
SFTRVFRWPL GIPQYNLGHL ERVAAIDAAL QRLPGLHLIG NAYKGVGLND 450
CIRNAAQLAD ALVAGNTSHA P 471
Length:471
Mass (Da):49,388
Last modified:December 15, 1998 - v1
Checksum:i2E9909D464F81515
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27429 Genomic DNA. Translation: AAM28643.1.
AF098938 Genomic DNA. Translation: AAD13609.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27429 Genomic DNA. Translation: AAM28643.1 .
AF098938 Genomic DNA. Translation: AAD13609.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IVD X-ray 2.30 A/B 4-471 [» ]
2IVE X-ray 2.70 A/B 4-471 [» ]
ProteinModelPortali P56601.
SMRi P56601. Positions 10-464.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
BRENDAi 1.3.3.4. 3551.
SABIO-RK P56601.

Miscellaneous databases

EvolutionaryTracei P56601.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "csgA expression entrains Myxococcus xanthus development."
    Li S., Lee B.U., Shimkets L.J.
    Genes Dev. 6:401-410(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Shimkets L.J.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. "Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme."
    Dailey H.A., Dailey T.A.
    J. Biol. Chem. 271:8714-8718(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, COFACTOR, ENZYME REGULATION, SUBUNIT.
  4. "Crystal structure of protoporphyrinogen oxidase from Myxococcus xanthus and its complex with the inhibitor acifluorfen."
    Corradi H.R., Corrigall A.V., Boix E., Mohan C.G., Sturrock E.D., Meissner P.N., Acharya K.R.
    J. Biol. Chem. 281:38625-38633(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 4-471 IN COMPLEX WITH FAD AND ACIFLUORFEN, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPPOX_MYXXA
AccessioniPrimary (citable) accession number: P56601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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