Reviewed, UniProtKB/Swiss-Prot P56598 (GST29_FASHE)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase class-mu 26 kDa isozyme 1       Short name=GST1     EC=2.5.1.18 Alternative name(s):     Fh1
Organism	Fasciola hepatica (Liver fluke)
Taxonomic identifier	6192 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Echinostomida › Echinostomata › Echinostomatoidea › Fasciolidae › Fasciola

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 221

220

Glutathione S-transferase class-mu 26 kDa isozyme 1

PRO_0000185810

Regions

Domain

2 – 82

81

GST N-terminal

Domain

84 – 202

119

GST C-terminal

Sites

Active site

7

1

By similarity

Experimental info

Sequence conflict

23

1

Y → V in CAA00118. Ref.3

Sequence conflict

111 – 112

2

DP → VS in CAA00118. Ref.3

Sequence conflict

190

1

A → P in CAA00118. Ref.3

Secondary structure

1

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221

Helix Strand Turn

Details...

Beta strand

3 – 11

9

Turn

12 – 14

3

Helix

15 – 23

9

Beta strand

27 – 32

6

Helix

37 – 43

7

Turn

44 – 46

3

Beta strand

54 – 58

5

Beta strand

63 – 66

4

Helix

67 – 77

11

Helix

85 – 109

25

Helix

114 – 136

23

Beta strand

144 – 146

3

Helix

149 – 164

16

Turn

166 – 171

6

Helix

173 – 183

11

Helix

186 – 192

7

Beta strand

194 – 196

3

Beta strand

208 – 210

3

Beta strand

212 – 214

3

Sequences

	Sequence		Length	Mass (Da)
	P56598-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 17EAF460F750895F	FASTA	221	25,730
	10 20 30 40 50 60 MPAKLGYWKI RGLQQPVRLL LEYGEKYEEQ IYERDDGEKW FSKKFELGLD LPNLPYYIDD 70 80 90 100 110 120 KCKLTQSLAI LRYIADKHGM IGSTPEERAR VSMIEGAAVD LRQGLSRISY DPKFEQLKEG 130 140 150 160 170 180 YLKDLPTTMK MWSDFLGKNP YLRGTSVSHV DFMVYEALDA IRYLEPHCLD HFPNLQQFMS 190 200 210 220 RIEALPSIKA YMESNRFIKW PLNGWHAQFG GGDAPPSHEK K

References

[1]	"Molecular characterization of cDNA sequences encoding glutathione S-transferases of Fasciola hepatica." Panaccio M., Wilson L.R., Crameri S.L., Wijffels G.L., Spithill T.W. Exp. Parasitol. 74:232-237(1992) [PubMed: 1740183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Panaccio M., Wilson L.R., Crameri S.L., Wijffels G.L., Spithill T.W. Exp. Parasitol. 77:385-385(1993) [PubMed: 8224094] [Abstract]
[3]	Crameri S. Patent number WO9008819, 09-AUG-1990 Cited for: NUCLEOTIDE SEQUENCE OF 23-221.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

A00993 Unassigned RNA. Translation: CAA00118.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FHE	X-ray	2.30	A/B	2-216	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

2.5.1.18. 39370.

Family and domain databases

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR003081. GST_mu.
IPR012336. Thioredoxin-like_fold.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

PRINTS

PR01267. GSTRNSFRASEM.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GST29_FASHE

Accession

Primary (citable) accession number: P56598

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)