P56594 (CP2CL_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C21
EC=1.14.14.1

Alternative name(s):
CYPIIC21
Cytochrome P450 DM1-1

Gene names

Name:

CYP2C21

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	487 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Showed testosterone hydrolase activity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 487	›487	Cytochrome P450 2C21		PRO_0000051710
Sites
	Metal binding	432	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	337 – 342	6	Missing no nucleotide entry Ref.2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P56594 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: E1390CACC7DD577D	FASTA	487	55,531
	10 20 30 40 50 60 FIVLVICLSC LISFFLWNQN RAKGKLPPGP TPLPIIGNIL QINTKNVSKS LSKLAENYGP 70 80 90 100 110 120 VFTVYFGMKP TVVLYGYEAV KEALIDRSEE FSGRGHFPLL DWTIQGLGIV FSNGEKWKQT 130 140 150 160 170 180 RRFSLTVLRN MGMGKKTVED RIQEEALYLV EALKKTNASP CDPTFLLGCA PCNVICSIIF 190 200 210 220 230 240 QNRFEYDDKD FLTLLEYFHE NLLISSTSWI QLYNAFPLLI HYLPGSHHVL FKNIANQFKF 250 260 270 280 290 300 ISEKIKEHEE SLNFSNPRDF IDYFLIKIEK EKHNKQSEFT MDNLIITIWD VFSAGTETTS 310 320 330 340 350 360 TTLRYGLLVL LKHPDVTAKV QEEIHRVVGR HRSPCMQDRS CMPYTDAVVH EIQRYIDLVP 370 380 390 400 410 420 NNLPHSVTQD IKFREYLIPK GTTILTSLTS VLHDEKGFPN PDQFDPGHFL DENGSFKKSD 430 440 450 460 470 480 YFMAFSAGKR VCVGEGLARM ELFLLLTNIL QHFTLKPLVD PKDIDTTPIA NGLGATPPSY KLCFVPV

References

[1]	"Isolation of a new canine cytochrome P450 cDNA from the cytochrome P450 2C subfamily (CYP2C41) and evidence for polymorphic differences in its expression." Blaisdell J., Goldstein J.A., Bai S.A. Drug Metab. Dispos. 26:278-283(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Beagle. Tissue: Liver.
[2]	"Isolation of cDNAs coding for three different forms of liver microsomal cytochrome P-450 from polychlorinated biphenyl-treated beagle dogs." Uchida T., Komori M., Kitada M., Kamataki T. Mol. Pharmacol. 38:644-651(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-487. Strain: Beagle. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF049909 mRNA. Translation: AAC05209.1.
UniGene	Cfa.3741.
3D structure databases
ProteinModelPortal	P56594.
SMR	P56594. Positions 27-487.
ModBase	Search...
Proteomic databases
PaxDb	P56594.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	P56594.
OrthoDB	EOG48WC22.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2CL_CANFA

Accession

Primary (citable) accession number: P56594
Secondary accession number(s): O62734

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program