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P56593 (CP2AC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome P450 2A12
EC=1.14.14.1
Alternative name(s):
CYPIIA12
Steroid hormones 7-alpha-hydroxylase
Testosterone 7-alpha-hydroxylase
Gene names
Name:Cyp2a12
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Highly active in the 7-alpha-hydroxylation of testosterone.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Liver.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Cytochrome P450 2A12
PRO_0000051675

Sites

Metal binding4371Iron (heme axial ligand) By similarity

Amino acid modifications

Modified residue1301Phosphoserine Ref.4

Experimental info

Mutagenesis2061Q → G: Same as wild-type.
Mutagenesis2081N → L: Stabilizes the protein, 17-fold lower Vmax.
Mutagenesis2091K → Q: Same as wild-type.
Sequence conflict1471R → H in L06463. Ref.1
Sequence conflict3751D → N in L06463. Ref.1
Sequence conflict3861G → A in L06463. Ref.1
Sequence conflict449 – 4502LF → FL in L06463. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P56593 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 38FD449996825F44

FASTA49256,179
        10         20         30         40         50         60 
MLGSGLLLLA ILAFLSVMVL VSVWQQKIRG KLPPGPIPLP FIGNYLQLNR KDVYSSITQL 

        70         80         90        100        110        120 
QEHYGPVFTI HLGPRRVVVL YGYDAVKEAL VDHAEEFSGR GEQATFNTLF KGYGVAFSNG 

       130        140        150        160        170        180 
ERAKQLRRFS IATLRDFGMG KRGVEERIQE EAGCLIKMLQ GTCGAPIDPT IYLSKTASNV 

       190        200        210        220        230        240 
ISSIVFGDRF NYEDKEFLSL LQMMGQVNKF AASPTGQLYD MFHSVMKYLP GPQQQIIKDS 

       250        260        270        280        290        300 
HKLEDFMIQK VKQNQSTLDP NSPRDFIDSF LIHMQKEKYV NSEFHMKNLV MTSLNLFFAG 

       310        320        330        340        350        360 
SETVSSTLRY GFLLLMKHPD VEAKVHEEID RVIGRNRQPQ YEDHMKMPYT QAVINEIQRF 

       370        380        390        400        410        420 
SNFAPLGIPR RITKDTSFRG FFLPKGTEVF PILGSLMTDP KFFSSPKDFN PQHFLDDKGQ 

       430        440        450        460        470        480 
LKKIPAFLPF STGKRFCLGD SLAKMELFLF FTTILQNFRF KFPRKLEDIN ESPTPEGFTR 

       490 
IIPKYTMSFV PI

« Hide

References

« Hide 'large scale' references
[1]"Site-directed mutagenesis of mouse steroid 7 alpha-hydroxylase (cytochrome P-450(7) alpha): role of residue-209 in determining steroid-cytochrome P-450 interaction."
Iwasaki M., Lindberg R.L.P., Juvonen R.O., Negishi M.
Biochem. J. 291:569-573(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
Strain: AKR/J.
Tissue: Liver.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[4]"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS."
Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y.
Mol. Cell. Proteomics 6:669-676(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-130, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L06463 mRNA. No translation available.
CH466593 Genomic DNA. Translation: EDL24208.1.
BC018356 mRNA. Translation: AAH18356.1.
BC094017 mRNA. Translation: AAH94017.1.
IPIIPI00308919.
PIRS32491.
RefSeqNP_598418.1. NM_133657.1.
UniGeneMm.20770.

3D structure databases

HSSPHSSP built from PDB template 1PO5 based on UniProtKB P00178.
ProteinModelPortalP56593.
SMRP56593. Positions 30-491.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000074990.

PTM databases

PhosphoSiteP56593.

Proteomic databases

PaxDbP56593.
PRIDEP56593.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075552; ENSMUSP00000074990; ENSMUSG00000060407.
GeneID13085.
KEGGmmu:13085.

Organism-specific databases

CTD13085.
MGIMGI:105055. Cyp2a12.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00670000097868.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidQ8VCW9.
KOK07411.
OMAPTIYLSK.
OrthoDBEOG4WM4WJ.

Gene expression databases

BgeeP56593.
GenevestigatorP56593.
GermOnlineENSMUSG00000060407. Mus musculus.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283046.
SOURCESearch...

Entry information

Entry nameCP2AC_MOUSE
AccessionPrimary (citable) accession number: P56593
Secondary accession number(s): Q8VCW9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: April 3, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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