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P56593

- CP2AC_MOUSE

UniProt

P56593 - CP2AC_MOUSE

Protein

Cytochrome P450 2A12

Gene

Cyp2a12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Highly active in the 7-alpha-hydroxylation of testosterone.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2A12 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIA12
    Steroid hormones 7-alpha-hydroxylase
    Testosterone 7-alpha-hydroxylase
    Gene namesi
    Name:Cyp2a12
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:105055. Cyp2a12.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi206 – 2061Q → G: Same as wild-type. 1 Publication
    Mutagenesisi208 – 2081N → L: Stabilizes the protein, 17-fold lower Vmax. 1 Publication
    Mutagenesisi209 – 2091K → Q: Same as wild-type. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Cytochrome P450 2A12PRO_0000051675Add
    BLAST

    Proteomic databases

    MaxQBiP56593.
    PaxDbiP56593.
    PRIDEiP56593.

    PTM databases

    PhosphoSiteiP56593.

    Expressioni

    Tissue specificityi

    Liver.

    Gene expression databases

    BgeeiP56593.
    GenevestigatoriP56593.

    Interactioni

    Protein-protein interaction databases

    IntActiP56593. 1 interaction.
    MINTiMINT-4119632.
    STRINGi10090.ENSMUSP00000074990.

    Structurei

    3D structure databases

    ProteinModelPortaliP56593.
    SMRiP56593. Positions 30-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00670000097868.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiQ8VCW9.
    KOiK07411.
    OMAiGSLMTDP.
    OrthoDBiEOG7RBZ85.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56593-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGSGLLLLA ILAFLSVMVL VSVWQQKIRG KLPPGPIPLP FIGNYLQLNR    50
    KDVYSSITQL QEHYGPVFTI HLGPRRVVVL YGYDAVKEAL VDHAEEFSGR 100
    GEQATFNTLF KGYGVAFSNG ERAKQLRRFS IATLRDFGMG KRGVEERIQE 150
    EAGCLIKMLQ GTCGAPIDPT IYLSKTASNV ISSIVFGDRF NYEDKEFLSL 200
    LQMMGQVNKF AASPTGQLYD MFHSVMKYLP GPQQQIIKDS HKLEDFMIQK 250
    VKQNQSTLDP NSPRDFIDSF LIHMQKEKYV NSEFHMKNLV MTSLNLFFAG 300
    SETVSSTLRY GFLLLMKHPD VEAKVHEEID RVIGRNRQPQ YEDHMKMPYT 350
    QAVINEIQRF SNFAPLGIPR RITKDTSFRG FFLPKGTEVF PILGSLMTDP 400
    KFFSSPKDFN PQHFLDDKGQ LKKIPAFLPF STGKRFCLGD SLAKMELFLF 450
    FTTILQNFRF KFPRKLEDIN ESPTPEGFTR IIPKYTMSFV PI 492
    Length:492
    Mass (Da):56,179
    Last modified:July 27, 2011 - v2
    Checksum:i38FD449996825F44
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti147 – 1471R → H in L06463. (PubMed:8484736)Curated
    Sequence conflicti375 – 3751D → N in L06463. (PubMed:8484736)Curated
    Sequence conflicti386 – 3861G → A in L06463. (PubMed:8484736)Curated
    Sequence conflicti449 – 4502LF → FL in L06463. (PubMed:8484736)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06463 mRNA. No translation available.
    CH466593 Genomic DNA. Translation: EDL24208.1.
    BC018356 mRNA. Translation: AAH18356.1.
    BC094017 mRNA. Translation: AAH94017.1.
    CCDSiCCDS21009.1.
    PIRiS32491.
    RefSeqiNP_598418.1. NM_133657.1.
    UniGeneiMm.20770.

    Genome annotation databases

    EnsembliENSMUST00000075552; ENSMUSP00000074990; ENSMUSG00000060407.
    GeneIDi13085.
    KEGGimmu:13085.
    UCSCiuc009fux.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06463 mRNA. No translation available.
    CH466593 Genomic DNA. Translation: EDL24208.1 .
    BC018356 mRNA. Translation: AAH18356.1 .
    BC094017 mRNA. Translation: AAH94017.1 .
    CCDSi CCDS21009.1.
    PIRi S32491.
    RefSeqi NP_598418.1. NM_133657.1.
    UniGenei Mm.20770.

    3D structure databases

    ProteinModelPortali P56593.
    SMRi P56593. Positions 30-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P56593. 1 interaction.
    MINTi MINT-4119632.
    STRINGi 10090.ENSMUSP00000074990.

    PTM databases

    PhosphoSitei P56593.

    Proteomic databases

    MaxQBi P56593.
    PaxDbi P56593.
    PRIDEi P56593.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000075552 ; ENSMUSP00000074990 ; ENSMUSG00000060407 .
    GeneIDi 13085.
    KEGGi mmu:13085.
    UCSCi uc009fux.1. mouse.

    Organism-specific databases

    CTDi 13085.
    MGIi MGI:105055. Cyp2a12.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00670000097868.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi Q8VCW9.
    KOi K07411.
    OMAi GSLMTDP.
    OrthoDBi EOG7RBZ85.
    TreeFami TF352043.

    Miscellaneous databases

    NextBioi 283046.
    PROi P56593.
    SOURCEi Search...

    Gene expression databases

    Bgeei P56593.
    Genevestigatori P56593.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01684. EP450ICYP2A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Site-directed mutagenesis of mouse steroid 7 alpha-hydroxylase (cytochrome P-450(7) alpha): role of residue-209 in determining steroid-cytochrome P-450 interaction."
      Iwasaki M., Lindberg R.L.P., Juvonen R.O., Negishi M.
      Biochem. J. 291:569-573(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
      Strain: AKR/J.
      Tissue: Liver.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP2AC_MOUSE
    AccessioniPrimary (citable) accession number: P56593
    Secondary accession number(s): Q8VCW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3