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Protein

Cytochrome P450 2A12

Gene

Cyp2a12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Highly active in the 7-alpha-hydroxylation of testosterone.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: GO_Central
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  6. oxygen binding Source: GO_Central
  7. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. coumarin metabolic process Source: GO_Central
  2. epoxygenase P450 pathway Source: GO_Central
  3. exogenous drug catabolic process Source: GO_Central
  4. oxidation-reduction process Source: GO_Central
  5. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_237136. Aflatoxin activation and detoxification.
REACT_245291. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A12 (EC:1.14.14.1)
Alternative name(s):
CYPIIA12
Steroid hormones 7-alpha-hydroxylase
Testosterone 7-alpha-hydroxylase
Gene namesi
Name:Cyp2a12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:105055. Cyp2a12.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi206 – 2061Q → G: Same as wild-type. 1 Publication
Mutagenesisi208 – 2081N → L: Stabilizes the protein, 17-fold lower Vmax. 1 Publication
Mutagenesisi209 – 2091K → Q: Same as wild-type. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Cytochrome P450 2A12PRO_0000051675Add
BLAST

Proteomic databases

MaxQBiP56593.
PaxDbiP56593.
PRIDEiP56593.

PTM databases

PhosphoSiteiP56593.

Expressioni

Tissue specificityi

Liver.

Gene expression databases

BgeeiP56593.
GenevestigatoriP56593.

Interactioni

Protein-protein interaction databases

IntActiP56593. 1 interaction.
MINTiMINT-4119632.
STRINGi10090.ENSMUSP00000074990.

Structurei

3D structure databases

ProteinModelPortaliP56593.
SMRiP56593. Positions 30-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP56593.
KOiK07411.
OMAiGSLMTDP.
OrthoDBiEOG7RBZ85.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGSGLLLLA ILAFLSVMVL VSVWQQKIRG KLPPGPIPLP FIGNYLQLNR
60 70 80 90 100
KDVYSSITQL QEHYGPVFTI HLGPRRVVVL YGYDAVKEAL VDHAEEFSGR
110 120 130 140 150
GEQATFNTLF KGYGVAFSNG ERAKQLRRFS IATLRDFGMG KRGVEERIQE
160 170 180 190 200
EAGCLIKMLQ GTCGAPIDPT IYLSKTASNV ISSIVFGDRF NYEDKEFLSL
210 220 230 240 250
LQMMGQVNKF AASPTGQLYD MFHSVMKYLP GPQQQIIKDS HKLEDFMIQK
260 270 280 290 300
VKQNQSTLDP NSPRDFIDSF LIHMQKEKYV NSEFHMKNLV MTSLNLFFAG
310 320 330 340 350
SETVSSTLRY GFLLLMKHPD VEAKVHEEID RVIGRNRQPQ YEDHMKMPYT
360 370 380 390 400
QAVINEIQRF SNFAPLGIPR RITKDTSFRG FFLPKGTEVF PILGSLMTDP
410 420 430 440 450
KFFSSPKDFN PQHFLDDKGQ LKKIPAFLPF STGKRFCLGD SLAKMELFLF
460 470 480 490
FTTILQNFRF KFPRKLEDIN ESPTPEGFTR IIPKYTMSFV PI
Length:492
Mass (Da):56,179
Last modified:July 27, 2011 - v2
Checksum:i38FD449996825F44
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471R → H in L06463 (PubMed:8484736).Curated
Sequence conflicti375 – 3751D → N in L06463 (PubMed:8484736).Curated
Sequence conflicti386 – 3861G → A in L06463 (PubMed:8484736).Curated
Sequence conflicti449 – 4502LF → FL in L06463 (PubMed:8484736).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06463 mRNA. No translation available.
CH466593 Genomic DNA. Translation: EDL24208.1.
BC018356 mRNA. Translation: AAH18356.1.
BC094017 mRNA. Translation: AAH94017.1.
CCDSiCCDS21009.1.
PIRiS32491.
RefSeqiNP_598418.1. NM_133657.1.
UniGeneiMm.20770.

Genome annotation databases

EnsembliENSMUST00000075552; ENSMUSP00000074990; ENSMUSG00000060407.
GeneIDi13085.
KEGGimmu:13085.
UCSCiuc009fux.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06463 mRNA. No translation available.
CH466593 Genomic DNA. Translation: EDL24208.1.
BC018356 mRNA. Translation: AAH18356.1.
BC094017 mRNA. Translation: AAH94017.1.
CCDSiCCDS21009.1.
PIRiS32491.
RefSeqiNP_598418.1. NM_133657.1.
UniGeneiMm.20770.

3D structure databases

ProteinModelPortaliP56593.
SMRiP56593. Positions 30-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56593. 1 interaction.
MINTiMINT-4119632.
STRINGi10090.ENSMUSP00000074990.

PTM databases

PhosphoSiteiP56593.

Proteomic databases

MaxQBiP56593.
PaxDbiP56593.
PRIDEiP56593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075552; ENSMUSP00000074990; ENSMUSG00000060407.
GeneIDi13085.
KEGGimmu:13085.
UCSCiuc009fux.1. mouse.

Organism-specific databases

CTDi13085.
MGIiMGI:105055. Cyp2a12.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP56593.
KOiK07411.
OMAiGSLMTDP.
OrthoDBiEOG7RBZ85.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_237136. Aflatoxin activation and detoxification.
REACT_245291. Xenobiotics.

Miscellaneous databases

NextBioi283046.
PROiP56593.
SOURCEiSearch...

Gene expression databases

BgeeiP56593.
GenevestigatoriP56593.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Site-directed mutagenesis of mouse steroid 7 alpha-hydroxylase (cytochrome P-450(7) alpha): role of residue-209 in determining steroid-cytochrome P-450 interaction."
    Iwasaki M., Lindberg R.L.P., Juvonen R.O., Negishi M.
    Biochem. J. 291:569-573(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS.
    Strain: AKR/J.
    Tissue: Liver.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiCP2AC_MOUSE
AccessioniPrimary (citable) accession number: P56593
Secondary accession number(s): Q8VCW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: February 4, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.