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Protein

Cytochrome P450 2A12

Gene

Cyp2a12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Highly active in the 7-alpha-hydroxylation of testosterone.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi437Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-211981. Xenobiotics.
R-MMU-5423646. Aflatoxin activation and detoxification.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A12 (EC:1.14.14.1)
Alternative name(s):
CYPIIA12
Steroid hormones 7-alpha-hydroxylase
Testosterone 7-alpha-hydroxylase
Gene namesi
Name:Cyp2a12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:105055. Cyp2a12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi206Q → G: Same as wild-type. 1 Publication1
Mutagenesisi208N → L: Stabilizes the protein, 17-fold lower Vmax. 1 Publication1
Mutagenesisi209K → Q: Same as wild-type. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516751 – 492Cytochrome P450 2A12Add BLAST492

Proteomic databases

MaxQBiP56593.
PaxDbiP56593.
PeptideAtlasiP56593.
PRIDEiP56593.

PTM databases

iPTMnetiP56593.
PhosphoSitePlusiP56593.

Expressioni

Tissue specificityi

Liver.

Gene expression databases

BgeeiENSMUSG00000060407.
GenevisibleiP56593. MM.

Interactioni

Protein-protein interaction databases

IntActiP56593. 1 interactor.
MINTiMINT-4119632.
STRINGi10090.ENSMUSP00000074990.

Structurei

3D structure databases

ProteinModelPortaliP56593.
SMRiP56593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP56593.
KOiK07411.
OrthoDBiEOG091G0BT8.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGSGLLLLA ILAFLSVMVL VSVWQQKIRG KLPPGPIPLP FIGNYLQLNR
60 70 80 90 100
KDVYSSITQL QEHYGPVFTI HLGPRRVVVL YGYDAVKEAL VDHAEEFSGR
110 120 130 140 150
GEQATFNTLF KGYGVAFSNG ERAKQLRRFS IATLRDFGMG KRGVEERIQE
160 170 180 190 200
EAGCLIKMLQ GTCGAPIDPT IYLSKTASNV ISSIVFGDRF NYEDKEFLSL
210 220 230 240 250
LQMMGQVNKF AASPTGQLYD MFHSVMKYLP GPQQQIIKDS HKLEDFMIQK
260 270 280 290 300
VKQNQSTLDP NSPRDFIDSF LIHMQKEKYV NSEFHMKNLV MTSLNLFFAG
310 320 330 340 350
SETVSSTLRY GFLLLMKHPD VEAKVHEEID RVIGRNRQPQ YEDHMKMPYT
360 370 380 390 400
QAVINEIQRF SNFAPLGIPR RITKDTSFRG FFLPKGTEVF PILGSLMTDP
410 420 430 440 450
KFFSSPKDFN PQHFLDDKGQ LKKIPAFLPF STGKRFCLGD SLAKMELFLF
460 470 480 490
FTTILQNFRF KFPRKLEDIN ESPTPEGFTR IIPKYTMSFV PI
Length:492
Mass (Da):56,179
Last modified:July 27, 2011 - v2
Checksum:i38FD449996825F44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147R → H in L06463 (PubMed:8484736).Curated1
Sequence conflicti375D → N in L06463 (PubMed:8484736).Curated1
Sequence conflicti386G → A in L06463 (PubMed:8484736).Curated1
Sequence conflicti449 – 450LF → FL in L06463 (PubMed:8484736).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06463 mRNA. No translation available.
CH466593 Genomic DNA. Translation: EDL24208.1.
BC018356 mRNA. Translation: AAH18356.1.
BC094017 mRNA. Translation: AAH94017.1.
CCDSiCCDS21009.1.
PIRiS32491.
RefSeqiNP_598418.1. NM_133657.1.
UniGeneiMm.20770.

Genome annotation databases

EnsembliENSMUST00000075552; ENSMUSP00000074990; ENSMUSG00000060407.
GeneIDi13085.
KEGGimmu:13085.
UCSCiuc009fux.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06463 mRNA. No translation available.
CH466593 Genomic DNA. Translation: EDL24208.1.
BC018356 mRNA. Translation: AAH18356.1.
BC094017 mRNA. Translation: AAH94017.1.
CCDSiCCDS21009.1.
PIRiS32491.
RefSeqiNP_598418.1. NM_133657.1.
UniGeneiMm.20770.

3D structure databases

ProteinModelPortaliP56593.
SMRiP56593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP56593. 1 interactor.
MINTiMINT-4119632.
STRINGi10090.ENSMUSP00000074990.

PTM databases

iPTMnetiP56593.
PhosphoSitePlusiP56593.

Proteomic databases

MaxQBiP56593.
PaxDbiP56593.
PeptideAtlasiP56593.
PRIDEiP56593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075552; ENSMUSP00000074990; ENSMUSG00000060407.
GeneIDi13085.
KEGGimmu:13085.
UCSCiuc009fux.1. mouse.

Organism-specific databases

CTDi13085.
MGIiMGI:105055. Cyp2a12.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP56593.
KOiK07411.
OrthoDBiEOG091G0BT8.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiR-MMU-211981. Xenobiotics.
R-MMU-5423646. Aflatoxin activation and detoxification.

Miscellaneous databases

PROiP56593.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060407.
GenevisibleiP56593. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008067. Cyt_P450_E_grp-I_CYP2A-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01684. EP450ICYP2A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP2AC_MOUSE
AccessioniPrimary (citable) accession number: P56593
Secondary accession number(s): Q8VCW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.