Reviewed, UniProtKB/Swiss-Prot P56592 (CP1A2_CANFA)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A2
    EC=1.14.14.1
Alternative name(s):
    CYPIA2
    DAH2
    Cytochrome P450-D2

Gene names

Name:

CYP1A2

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Constitutively expressed in liver.
Induction	By polychlorinated biphenyl (PCB) in liver and kidney.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 512	511	Cytochrome P450 1A2		PRO_0000051649
Sites
	Metal binding	454	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	285	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56592-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E49DF4C54F4B3CFB	FASTA	512	57,637
	10 20 30 40 50 60 MALSQMATGL LLASTIFCLI LWVVKAWQPR LPKGLKSPPG PWGWPLLGNV LTLGKSPHLA 70 80 90 100 110 120 LSRLSQRYGD VLQIRIGSTP VLVLSGLDTI RQALVRQGDD FKGRPDLYSL SLITDSQSMS 130 140 150 160 170 180 FSPDSGPVWA AGRRLAQNAL NTFSIASDPA SSCSCYLEEH VSKEAEALLS RLQEQMAEVG 190 200 210 220 230 240 RFDPYNQVLL SVANVIGAMC FGHHFSQRSE EMLPLLMSSS DFVETVSSGN PLDFFPILQY 250 260 270 280 290 300 MPNSALQRFK NFNQTFVQSL QKIVQEHYQD FDERSVQDIT GALLKHNEKS SRASDGHIPQ 310 320 330 340 350 360 EKIVNLINDI FGAGFDTVTT AISWSLMYLV ANPEIQRQIQ KELDTVIGRA RQPRLSDRPQ 370 380 390 400 410 420 LPLMEAFILE IFRHTSFVPF TIPHSTTKDT TLKGFYIPKE CCVFINQWQV NHDQQVWGDP 430 440 450 460 470 480 FAFRPERFLT ADGTTINKTL SEKVMLFGMG KRRCIGEVLA KWEIFLFLAI LLQRLEFSVP 490 500 510 AGVKVDLTPI YGLTMKHTRC EHVQARPRFS IK

References

[1]	"A novel form of cytochrome P-450 in beagle dogs. P-450-D3 is a low spin form of cytochrome P-450 but with catalytic and structural properties similar to P-450d." Ohta K., Motoya M., Komori M., Miura T., Kitada M., Kamataki T. Biochem. Pharmacol. 38:91-96(1989) [PubMed: 2910310] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17. Strain: Beagle. Tissue: Liver.
[2]	"Isolation of cDNAs coding for three different forms of liver microsomal cytochrome P-450 from polychlorinated biphenyl-treated beagle dogs." Uchida T., Komori M., Kitada M., Kamataki T. Mol. Pharmacol. 38:644-651(1990) [PubMed: 2122230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-512. Strain: Beagle. Tissue: Liver.

Cross-references

Sequence databases
PIR	B37222.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	P56592. Positions 30-510.
ModBase	Search...
Protein-protein interaction databases
STRING	P56592.
Genome annotation databases
Ensembl	ENSCAFT00000039456; ENSCAFP00000035314; ENSCAFG00000017941; Canis familiaris. [Genome view]
Phylogenomic databases
HOVERGEN	P56592.
Enzyme and pathway databases
BRENDA	1.14.14.1. 463.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A2_CANFA

Accession

Primary (citable) accession number: P56592

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)