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Protein

Cytochrome P450 1A2

Gene

CYP1A2

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.By similarity

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei222SubstrateBy similarity1
Metal bindingi454Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1By similarity)
Alternative name(s):
CYPIA2
Cholesterol 25-hydroxylaseBy similarity
Cytochrome P450-D2
DAH2
Gene namesi
Name:CYP1A2
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000516492 – 512Cytochrome P450 1A2Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi65O-linked (GlcNAc)By similarity1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP56592.
PRIDEiP56592.

Expressioni

Tissue specificityi

Constitutively expressed in liver.

Inductioni

By polychlorinated biphenyl (PCB) in liver and kidney.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000035314.

Structurei

3D structure databases

ProteinModelPortaliP56592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOVERGENiHBG106944.
InParanoidiP56592.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSQMATGL LLASTIFCLI LWVVKAWQPR LPKGLKSPPG PWGWPLLGNV
60 70 80 90 100
LTLGKSPHLA LSRLSQRYGD VLQIRIGSTP VLVLSGLDTI RQALVRQGDD
110 120 130 140 150
FKGRPDLYSL SLITDSQSMS FSPDSGPVWA AGRRLAQNAL NTFSIASDPA
160 170 180 190 200
SSCSCYLEEH VSKEAEALLS RLQEQMAEVG RFDPYNQVLL SVANVIGAMC
210 220 230 240 250
FGHHFSQRSE EMLPLLMSSS DFVETVSSGN PLDFFPILQY MPNSALQRFK
260 270 280 290 300
NFNQTFVQSL QKIVQEHYQD FDERSVQDIT GALLKHNEKS SRASDGHIPQ
310 320 330 340 350
EKIVNLINDI FGAGFDTVTT AISWSLMYLV ANPEIQRQIQ KELDTVIGRA
360 370 380 390 400
RQPRLSDRPQ LPLMEAFILE IFRHTSFVPF TIPHSTTKDT TLKGFYIPKE
410 420 430 440 450
CCVFINQWQV NHDQQVWGDP FAFRPERFLT ADGTTINKTL SEKVMLFGMG
460 470 480 490 500
KRRCIGEVLA KWEIFLFLAI LLQRLEFSVP AGVKVDLTPI YGLTMKHTRC
510
EHVQARPRFS IK
Length:512
Mass (Da):57,637
Last modified:January 23, 2007 - v2
Checksum:iE49DF4C54F4B3CFB
GO

Sequence databases

PIRiB37222.
UniGeneiCfa.14521.

Cross-referencesi

Sequence databases

PIRiB37222.
UniGeneiCfa.14521.

3D structure databases

ProteinModelPortaliP56592.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000035314.

Proteomic databases

PaxDbiP56592.
PRIDEiP56592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOVERGENiHBG106944.
InParanoidiP56592.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP1A2_CANLF
AccessioniPrimary (citable) accession number: P56592
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.