Reviewed, UniProtKB/Swiss-Prot P56590 (CP1A1_CANFA)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A1
    EC=1.14.14.1
Alternative name(s):
    CYPIA1
    DAH1

Gene names

Name:

CYP1A1

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. This isozyme seems responsible for metabolism of 2,2',4,4',5,5'-hexachlorobiphenyl.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By polychlorinated biphenyl (PCB) in liver and kidney.
Sequence similarities	Belongs to the cytochrome P450 family.
Caution	It is uncertain whether Met-1, Met-2 or Met-4 is the initiator.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Cytochrome P450 1A1		PRO_0000051625
Sites
	Metal binding	461	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	364	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56590-1 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 0BBD2C651C13EB40	FASTA	524	59,209
	10 20 30 40 50 60 MMSMFRLSIP ISASELLLAS TVFCLVLWVV KAWQPRLPKG LKSPPGPWGW PVLGNVLTLG 70 80 90 100 110 120 KSPHLALSRL SQRYGDVLQI RIGSTPVLVL SGLDTIRQAL VRQGDDFKGR PDLYSFSLVT 130 140 150 160 170 180 DGQSLTFSPD SGPVWAARRR LAQNALKSFS IASDPASSCS CYLEEHVSKE AEVLLSRLQE 190 200 210 220 230 240 QMAEVGRFDP YRYIVVSVAN VICAMCFSKR YDHDDQELLS LVNLSNEFGE GVASANPLDF 250 260 270 280 290 300 FPILRYLPNP ALDFFKDLNK RFYSFMQKMV KEHYKTFEKG QIRDVTDSLI EHCQDKRLDE 310 320 330 340 350 360 NANIQLSDEK IVNVVLDLFG AGFDTVTTAI SWSLLYLVTN PNVQKKIQKE LDTVIGRARQ 370 380 390 400 410 420 PRLSDRPQLP YMEAFILETF RHASFVPFTI PHSTTRDTSL SGFYIPKGRC VFVNQWQINH 430 440 450 460 470 480 DQKLWGNPSE FQPERFLTLD GTINKALSEK VILFGLGKRK CIGETIARLE VFLFLAILLQ 490 500 510 520 QVEFSVPEGT KVDMTPIYGL TMKHARCEHF QVRVRTEGAE SPAA

References

[1]

"Isolation of cDNAs coding for three different forms of liver microsomal cytochrome P-450 from polychlorinated biphenyl-treated beagle dogs." Uchida T., Komori M., Kitada M., Kamataki T. Mol. Pharmacol. 38:644-651(1990) [PubMed: 2122230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Beagle. Tissue: Liver.

Cross-references

Sequence databases
PIR	C37222.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	P56590. Positions 36-513.
ModBase	Search...
Protein-protein interaction databases
STRING	P56590.
Genome annotation databases
Ensembl	ENSCAFT00000028474; ENSCAFP00000026483; ENSCAFG00000017937; Canis familiaris. [Genome view]
Phylogenomic databases
HOVERGEN	P56590.
Enzyme and pathway databases
BRENDA	1.14.14.1. 463.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A1_CANFA

Accession

Primary (citable) accession number: P56590

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	October 13, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)