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Protein

Glucitol/sorbitol-specific phosphotransferase enzyme IIB component

Gene

srlE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucitol/sorbitol transport.

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-sorbitol(Side 1) = [protein]-L-histidine + D-sorbitol 6-phosphate(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Phosphocysteine intermediate; for EIIB activityBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate transmembrane transport Source: GOC
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GUTA-MONOMER.
ECOL316407:JW5430-MONOMER.
MetaCyc:GUTA-MONOMER.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucitol/sorbitol-specific phosphotransferase enzyme IIB component (EC:2.7.1.198)
Alternative name(s):
EII-Gut
PTS system glucitol/sorbitol-specific EIIB component
Gene namesi
Name:srlE
Synonyms:gutE
Ordered Locus Names:b2703, JW5430
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14373. srlE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 176176CytoplasmicSequence analysisAdd
BLAST
Transmembranei177 – 19721HelicalSequence analysisAdd
BLAST
Topological domaini198 – 1992PeriplasmicSequence analysis
Transmembranei200 – 22021HelicalSequence analysisAdd
BLAST
Topological domaini221 – 2288CytoplasmicSequence analysis
Transmembranei229 – 24921HelicalSequence analysisAdd
BLAST
Topological domaini250 – 2567PeriplasmicSequence analysis
Transmembranei257 – 27721HelicalSequence analysisAdd
BLAST
Topological domaini278 – 29619CytoplasmicSequence analysisAdd
BLAST
Transmembranei297 – 31721HelicalSequence analysisAdd
BLAST
Topological domaini318 – 3192PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Glucitol/sorbitol-specific phosphotransferase enzyme IIB componentPRO_0000186562Add
BLAST

Proteomic databases

PaxDbiP56580.

Interactioni

Protein-protein interaction databases

BioGridi4262075. 19 interactions.
STRINGi511145.b2703.

Structurei

3D structure databases

ProteinModelPortaliP56580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 178178PTS EIIB type-5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni179 – 319141UnknownAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-5 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DZY. Bacteria.
COG3732. LUCA.
HOGENOMiHOG000242653.
InParanoidiP56580.
KOiK02782.
K02783.
OMAiALAIVDC.
OrthoDBiEOG68DD01.
PhylomeDBiP56580.

Family and domain databases

InterProiIPR011638. PTS_EIIBC_GUT_C.
IPR011618. PTS_EIIBC_GUT_N.
IPR004702. PTS_sorb_EIIBC.
[Graphical view]
PfamiPF07663. EIIBC-GUT_C. 1 hit.
PF03612. EIIBC-GUT_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00825. EIIBC-GUT. 1 hit.
PROSITEiPS51102. PTS_EIIB_TYPE_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHIRIEKGT GGWGGPLELK ATPGKKIVYI TAGTRPAIVD KLAQLTGWQA
60 70 80 90 100
IDGFKEGEPA EAEIGVAVID CGGTLRCGIY PKRRIPTINI HSTGKSGPLA
110 120 130 140 150
QYIVEDIYVS GVKEENITVV GDATPQPSSV GRDYDTSKKI TEQSDGLLAK
160 170 180 190 200
VGMGMGSTVA VLFQSGRDTI DTVLKTILPF MAFVSALIGI IMASGLGDWI
210 220 230 240 250
AHGLAPLASH PLGLVMLALI CSFPLLSPFL GPGAVIAQVI GVLIGVQIGL
260 270 280 290 300
GNIPPHLALP ALFAINAQAA CDFIPVGLSL AEARQDTVRV GVPSVLVSRF
310
LTGAPTVLIA WFVSGFIYQ
Length:319
Mass (Da):33,332
Last modified:December 15, 1998 - v1
Checksum:i3680B56EB625499F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13416.1.
U00096 Genomic DNA. Translation: AAT48149.1.
AP009048 Genomic DNA. Translation: BAA16564.2.
PIRiA26725. WQEC2S.
RefSeqiWP_000148878.1. NZ_LN832404.1.
YP_026181.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48149; AAT48149; b2703.
BAA16564; BAA16564; BAA16564.
GeneIDi948933.
KEGGiecj:JW5430.
eco:b2703.
PATRICi32120804. VBIEscCol129921_2794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13416.1.
U00096 Genomic DNA. Translation: AAT48149.1.
AP009048 Genomic DNA. Translation: BAA16564.2.
PIRiA26725. WQEC2S.
RefSeqiWP_000148878.1. NZ_LN832404.1.
YP_026181.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP56580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262075. 19 interactions.
STRINGi511145.b2703.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Proteomic databases

PaxDbiP56580.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48149; AAT48149; b2703.
BAA16564; BAA16564; BAA16564.
GeneIDi948933.
KEGGiecj:JW5430.
eco:b2703.
PATRICi32120804. VBIEscCol129921_2794.

Organism-specific databases

EchoBASEiEB4116.
EcoGeneiEG14373. srlE.

Phylogenomic databases

eggNOGiENOG4105DZY. Bacteria.
COG3732. LUCA.
HOGENOMiHOG000242653.
InParanoidiP56580.
KOiK02782.
K02783.
OMAiALAIVDC.
OrthoDBiEOG68DD01.
PhylomeDBiP56580.

Enzyme and pathway databases

BioCyciEcoCyc:GUTA-MONOMER.
ECOL316407:JW5430-MONOMER.
MetaCyc:GUTA-MONOMER.

Miscellaneous databases

PROiP56580.

Family and domain databases

InterProiIPR011638. PTS_EIIBC_GUT_C.
IPR011618. PTS_EIIBC_GUT_N.
IPR004702. PTS_sorb_EIIBC.
[Graphical view]
PfamiPF07663. EIIBC-GUT_C. 1 hit.
PF03612. EIIBC-GUT_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00825. EIIBC-GUT. 1 hit.
PROSITEiPS51102. PTS_EIIB_TYPE_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. Nucleotide sequence of the gut operon."
    Yamada M., Saier M.H. Jr.
    J. Biol. Chem. 262:5455-5463(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The glucitol permease of Escherichia coli: a tripartite permease of the phosphotransferase system."
    Reizer J., Reizer A., Yamada M., Saier M.H. Jr.
    Microbiology 144:1463-1464(1998)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: SEQUENCE REVISION TO 128 AND 230.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPTHB_ECOLI
AccessioniPrimary (citable) accession number: P56580
Secondary accession number(s): P05705, P78103, P78215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.