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Protein

PTS system glucitol/sorbitol-specific EIIB component

Gene

srlE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport. It can also use D-mannitol.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-sorbitol(Side 1) = [protein]-L-histidine + D-sorbitol 6-phosphate(Side 2).1 Publication

Kineticsi

  1. KM=44 µM for D-glucitol1 Publication
  2. KM=60 µM for D-mannitol1 Publication
  1. Vmax=7.2 nmol/min/mg enzyme with D-mannitol as substrate1 Publication
  2. Vmax=0.83 nmol/min/mg enzyme with D-glucitol as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Phosphocysteine intermediate; for EIIB activityCurated1

GO - Molecular functioni

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GUTA-MONOMER.
ECOL316407:JW5430-MONOMER.
MetaCyc:GUTA-MONOMER.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucitol/sorbitol-specific EIIB component1 Publication (EC:2.7.1.1981 Publication)
Alternative name(s):
EII-Gut1 Publication
Enzyme II-Gut1 Publication
Glucitol/sorbitol-specific phosphotransferase enzyme IIB component1 Publication
Gene namesi
Name:srlE
Synonyms:gutA1 Publication, gutE
Ordered Locus Names:b2703, JW5430
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14373. srlE.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 176CytoplasmicSequence analysisAdd BLAST176
Transmembranei177 – 197HelicalSequence analysisAdd BLAST21
Topological domaini198 – 199PeriplasmicSequence analysis2
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 228CytoplasmicSequence analysis8
Transmembranei229 – 249HelicalSequence analysisAdd BLAST21
Topological domaini250 – 256PeriplasmicSequence analysis7
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 296CytoplasmicSequence analysisAdd BLAST19
Transmembranei297 – 317HelicalSequence analysisAdd BLAST21
Topological domaini318 – 319PeriplasmicSequence analysis2

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865621 – 319PTS system glucitol/sorbitol-specific EIIB componentAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP56580.
PRIDEiP56580.

Expressioni

Inductioni

Regulated by an unusual system which consists of the activator GutM and the repressor GutR in addition to the cAMP-CRP complex.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262075. 19 interactors.
STRINGi511145.b2703.

Structurei

3D structure databases

ProteinModelPortaliP56580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 178PTS EIIB type-5PROSITE-ProRule annotationAdd BLAST178

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIB type-5 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DZY. Bacteria.
COG3732. LUCA.
HOGENOMiHOG000242653.
InParanoidiP56580.
KOiK02782.
K02783.
OMAiALAIVDC.
PhylomeDBiP56580.

Family and domain databases

InterProiIPR011638. PTS_EIIBC_GUT_C.
IPR011618. PTS_EIIBC_GUT_N.
IPR004702. PTS_sorb_EIIBC.
[Graphical view]
PfamiPF07663. EIIBC-GUT_C. 1 hit.
PF03612. EIIBC-GUT_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00825. EIIBC-GUT. 1 hit.
PROSITEiPS51102. PTS_EIIB_TYPE_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHIRIEKGT GGWGGPLELK ATPGKKIVYI TAGTRPAIVD KLAQLTGWQA
60 70 80 90 100
IDGFKEGEPA EAEIGVAVID CGGTLRCGIY PKRRIPTINI HSTGKSGPLA
110 120 130 140 150
QYIVEDIYVS GVKEENITVV GDATPQPSSV GRDYDTSKKI TEQSDGLLAK
160 170 180 190 200
VGMGMGSTVA VLFQSGRDTI DTVLKTILPF MAFVSALIGI IMASGLGDWI
210 220 230 240 250
AHGLAPLASH PLGLVMLALI CSFPLLSPFL GPGAVIAQVI GVLIGVQIGL
260 270 280 290 300
GNIPPHLALP ALFAINAQAA CDFIPVGLSL AEARQDTVRV GVPSVLVSRF
310
LTGAPTVLIA WFVSGFIYQ
Length:319
Mass (Da):33,332
Last modified:December 15, 1998 - v1
Checksum:i3680B56EB625499F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13416.1.
U00096 Genomic DNA. Translation: AAT48149.1.
AP009048 Genomic DNA. Translation: BAA16564.2.
PIRiA26725. WQEC2S.
RefSeqiWP_000148878.1. NZ_LN832404.1.
YP_026181.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48149; AAT48149; b2703.
BAA16564; BAA16564; BAA16564.
GeneIDi948933.
KEGGiecj:JW5430.
eco:b2703.
PATRICi32120804. VBIEscCol129921_2794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13416.1.
U00096 Genomic DNA. Translation: AAT48149.1.
AP009048 Genomic DNA. Translation: BAA16564.2.
PIRiA26725. WQEC2S.
RefSeqiWP_000148878.1. NZ_LN832404.1.
YP_026181.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP56580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262075. 19 interactors.
STRINGi511145.b2703.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Proteomic databases

PaxDbiP56580.
PRIDEiP56580.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48149; AAT48149; b2703.
BAA16564; BAA16564; BAA16564.
GeneIDi948933.
KEGGiecj:JW5430.
eco:b2703.
PATRICi32120804. VBIEscCol129921_2794.

Organism-specific databases

EchoBASEiEB4116.
EcoGeneiEG14373. srlE.

Phylogenomic databases

eggNOGiENOG4105DZY. Bacteria.
COG3732. LUCA.
HOGENOMiHOG000242653.
InParanoidiP56580.
KOiK02782.
K02783.
OMAiALAIVDC.
PhylomeDBiP56580.

Enzyme and pathway databases

BioCyciEcoCyc:GUTA-MONOMER.
ECOL316407:JW5430-MONOMER.
MetaCyc:GUTA-MONOMER.

Miscellaneous databases

PROiP56580.

Family and domain databases

InterProiIPR011638. PTS_EIIBC_GUT_C.
IPR011618. PTS_EIIBC_GUT_N.
IPR004702. PTS_sorb_EIIBC.
[Graphical view]
PfamiPF07663. EIIBC-GUT_C. 1 hit.
PF03612. EIIBC-GUT_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00825. EIIBC-GUT. 1 hit.
PROSITEiPS51102. PTS_EIIB_TYPE_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTHB_ECOLI
AccessioniPrimary (citable) accession number: P56580
Secondary accession number(s): P05705, P78103, P78215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.