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Protein

PTS system glucitol/sorbitol-specific EIIB component

Gene

srlE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport. It can also use D-mannitol.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-sorbitol(Side 1) = [protein]-L-histidine + D-sorbitol 6-phosphate(Side 2).1 Publication

Kineticsi

  1. KM=44 µM for D-glucitol1 Publication
  2. KM=60 µM for D-mannitol1 Publication
  1. Vmax=7.2 nmol/min/mg enzyme with D-mannitol as substrate1 Publication
  2. Vmax=0.83 nmol/min/mg enzyme with D-glucitol as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Phosphocysteine intermediate; for EIIB activityCurated1

GO - Molecular functioni

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GUTA-MONOMER
MetaCyc:GUTA-MONOMER

Protein family/group databases

TCDBi4.A.4.1.1 the pts glucitol (gut) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucitol/sorbitol-specific EIIB component1 Publication (EC:2.7.1.1981 Publication)
Alternative name(s):
EII-Gut1 Publication
Enzyme II-Gut1 Publication
Glucitol/sorbitol-specific phosphotransferase enzyme IIB component1 Publication
Gene namesi
Name:srlE
Synonyms:gutA1 Publication, gutE
Ordered Locus Names:b2703, JW5430
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14373 srlE

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 176CytoplasmicSequence analysisAdd BLAST176
Transmembranei177 – 197HelicalSequence analysisAdd BLAST21
Topological domaini198 – 199PeriplasmicSequence analysis2
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 228CytoplasmicSequence analysis8
Transmembranei229 – 249HelicalSequence analysisAdd BLAST21
Topological domaini250 – 256PeriplasmicSequence analysis7
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 296CytoplasmicSequence analysisAdd BLAST19
Transmembranei297 – 317HelicalSequence analysisAdd BLAST21
Topological domaini318 – 319PeriplasmicSequence analysis2

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865621 – 319PTS system glucitol/sorbitol-specific EIIB componentAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP56580
PRIDEiP56580

Expressioni

Inductioni

Regulated by an unusual system which consists of the activator GutM and the repressor GutR in addition to the cAMP-CRP complex.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262075, 19 interactors
STRINGi316385.ECDH10B_2871

Structurei

3D structure databases

ProteinModelPortaliP56580
SMRiP56580
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 178PTS EIIB type-5PROSITE-ProRule annotationAdd BLAST178

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DZY Bacteria
COG3732 LUCA
HOGENOMiHOG000242653
InParanoidiP56580
KOiK02782
K02783
OMAiHKFIYIT
PhylomeDBiP56580

Family and domain databases

InterProiView protein in InterPro
IPR011638 PTS_EIIBC_GUT_C
IPR011618 PTS_EIIBC_GUT_N
IPR004702 PTS_sorb_EIIBC
PANTHERiPTHR39427 PTHR39427, 1 hit
PfamiView protein in Pfam
PF07663 EIIBC-GUT_C, 1 hit
PF03612 EIIBC-GUT_N, 1 hit
TIGRFAMsiTIGR00825 EIIBC-GUT, 1 hit
PROSITEiView protein in PROSITE
PS51102 PTS_EIIB_TYPE_5, 1 hit

Sequencei

Sequence statusi: Complete.

P56580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHIRIEKGT GGWGGPLELK ATPGKKIVYI TAGTRPAIVD KLAQLTGWQA
60 70 80 90 100
IDGFKEGEPA EAEIGVAVID CGGTLRCGIY PKRRIPTINI HSTGKSGPLA
110 120 130 140 150
QYIVEDIYVS GVKEENITVV GDATPQPSSV GRDYDTSKKI TEQSDGLLAK
160 170 180 190 200
VGMGMGSTVA VLFQSGRDTI DTVLKTILPF MAFVSALIGI IMASGLGDWI
210 220 230 240 250
AHGLAPLASH PLGLVMLALI CSFPLLSPFL GPGAVIAQVI GVLIGVQIGL
260 270 280 290 300
GNIPPHLALP ALFAINAQAA CDFIPVGLSL AEARQDTVRV GVPSVLVSRF
310
LTGAPTVLIA WFVSGFIYQ
Length:319
Mass (Da):33,332
Last modified:December 15, 1998 - v1
Checksum:i3680B56EB625499F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA Translation: AAC13416.1
U00096 Genomic DNA Translation: AAT48149.1
AP009048 Genomic DNA Translation: BAA16564.2
PIRiA26725 WQEC2S
RefSeqiWP_000148878.1, NZ_LN832404.1
YP_026181.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48149; AAT48149; b2703
BAA16564; BAA16564; BAA16564
GeneIDi948933
KEGGiecj:JW5430
eco:b2703
PATRICifig|1411691.4.peg.4039

Similar proteinsi

Entry informationi

Entry nameiPTHB_ECOLI
AccessioniPrimary (citable) accession number: P56580
Secondary accession number(s): P05705, P78103, P78215
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health