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Protein

Glucitol/sorbitol permease IIC component

Gene

srlA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in glucitol/sorbitol transport.

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G8210-MONOMER.
ECOL316407:JW5429-MONOMER.
MetaCyc:G8210-MONOMER.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucitol/sorbitol permease IIC component
Alternative name(s):
EIIC-Gut
PTS system glucitol/sorbitol-specific EIIC component
Gene namesi
Name:srlA
Synonyms:gutA, sbl
Ordered Locus Names:b2702, JW5429
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10969. srlA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828PeriplasmicSequence analysisAdd
BLAST
Transmembranei29 – 4921HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini50 – 6718CytoplasmicSequence analysisAdd
BLAST
Transmembranei68 – 8821HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini89 – 13143PeriplasmicSequence analysisAdd
BLAST
Transmembranei132 – 15221HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini153 – 18735CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Glucitol/sorbitol permease IIC componentPRO_0000186565Add
BLAST

Proteomic databases

PaxDbiP56579.

Interactioni

Protein-protein interaction databases

BioGridi4262078. 18 interactions.
IntActiP56579. 1 interaction.
STRINGi511145.b2702.

Structurei

3D structure databases

ProteinModelPortaliP56579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 187187PTS EIIC type-5PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similaritiesi

Contains 1 PTS EIIC type-5 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410787A. Bacteria.
COG3730. LUCA.
HOGENOMiHOG000242767.
InParanoidiP56579.
KOiK02783.
OMAiHINPGEY.
OrthoDBiEOG6FRD1T.
PhylomeDBiP56579.

Family and domain databases

InterProiIPR004699. PTS_IID_sorb.
[Graphical view]
PfamiPF03608. EII-GUT. 1 hit.
[Graphical view]
PIRSFiPIRSF038321. PTS_glc_srb_IIC. 1 hit.
TIGRFAMsiTIGR00821. EII-GUT. 1 hit.
PROSITEiPS51107. PTS_EIIC_TYPE_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56579-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETITHGAE WFIGLFQKGG EVFTGMVTGI LPLLISLLVI MNALINFIGQ
60 70 80 90 100
HRIERFAQRC AGNPVSRYLL LPCIGTFVFC NPMTLSLGRF MPEKYKPSYY
110 120 130 140 150
AAASYSCHSM NGLFPHINPG ELFVYLGIAS GLTTLNLPLG PLAVSYLLVG
160 170 180
LVTNFFRGWV TDLTTAIFEK KMGIQLEQKV HLAGATS
Length:187
Mass (Da):20,580
Last modified:December 15, 1998 - v1
Checksum:i86A10715BD9DE287
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13411.1.
U00096 Genomic DNA. Translation: AAT48148.1.
AP009048 Genomic DNA. Translation: BAA18886.2.
X70016 Genomic DNA. Translation: CAA49614.1.
PIRiA26725. WQEC2S.
RefSeqiWP_000573321.1. NZ_LN832404.1.
YP_026180.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48148; AAT48148; b2702.
BAA18886; BAA18886; BAA18886.
GeneIDi947575.
KEGGiecj:JW5429.
eco:b2702.
PATRICi32120802. VBIEscCol129921_2793.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13411.1.
U00096 Genomic DNA. Translation: AAT48148.1.
AP009048 Genomic DNA. Translation: BAA18886.2.
X70016 Genomic DNA. Translation: CAA49614.1.
PIRiA26725. WQEC2S.
RefSeqiWP_000573321.1. NZ_LN832404.1.
YP_026180.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP56579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262078. 18 interactions.
IntActiP56579. 1 interaction.
STRINGi511145.b2702.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Proteomic databases

PaxDbiP56579.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48148; AAT48148; b2702.
BAA18886; BAA18886; BAA18886.
GeneIDi947575.
KEGGiecj:JW5429.
eco:b2702.
PATRICi32120802. VBIEscCol129921_2793.

Organism-specific databases

EchoBASEiEB0962.
EcoGeneiEG10969. srlA.

Phylogenomic databases

eggNOGiENOG410787A. Bacteria.
COG3730. LUCA.
HOGENOMiHOG000242767.
InParanoidiP56579.
KOiK02783.
OMAiHINPGEY.
OrthoDBiEOG6FRD1T.
PhylomeDBiP56579.

Enzyme and pathway databases

BioCyciEcoCyc:G8210-MONOMER.
ECOL316407:JW5429-MONOMER.
MetaCyc:G8210-MONOMER.

Miscellaneous databases

PROiP56579.

Family and domain databases

InterProiIPR004699. PTS_IID_sorb.
[Graphical view]
PfamiPF03608. EII-GUT. 1 hit.
[Graphical view]
PIRSFiPIRSF038321. PTS_glc_srb_IIC. 1 hit.
TIGRFAMsiTIGR00821. EII-GUT. 1 hit.
PROSITEiPS51107. PTS_EIIC_TYPE_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. Nucleotide sequence of the gut operon."
    Yamada M., Saier M.H. Jr.
    J. Biol. Chem. 262:5455-5463(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The glucitol permease of Escherichia coli: a tripartite permease of the phosphotransferase system."
    Reizer J., Reizer A., Yamada M., Saier M.H. Jr.
    Microbiology 144:1463-1464(1998)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: SEQUENCE REVISION TO 21.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "A whole genome approach to in vivo DNA-protein interactions in E. coli."
    Wang M.X., Church G.M.
    Nature 360:606-610(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
    Strain: K12.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPTHC_ECOLI
AccessioniPrimary (citable) accession number: P56579
Secondary accession number(s): P05705, P78102, P78214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 15, 1998
Last modified: January 20, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.