Isocitrate dehydrogenase [NADP], mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot P56574 (IDHP_RAT)

Last modified November 25, 2008. Version 48. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP
    ICD-M

Gene names

Name:

Idh2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex By similarity.
Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion.
Miscellaneous	On the 2D-gel the determined pI of this protein (spot P8) is: 9.0, its MW is: 42 kDa.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

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Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 39

Mitochondrion By similarity

Chain

40 – 452

413

Isocitrate dehydrogenase [NADP], mitochondrial

PRO_0000083581

Regions

Nucleotide binding

115 – 117

NADP By similarity

Nucleotide binding

349 – 354

NADP By similarity

Region

134 – 140

Substrate binding By similarity

Sites

Metal binding

291

Magnesium or manganese By similarity

Metal binding

314

Magnesium or manganese By similarity

Binding site

117

Substrate By similarity

Binding site

122

NADP By similarity

Binding site

149

Substrate By similarity

Binding site

172

Substrate By similarity

Binding site

299

NADP By similarity

Binding site

367

NADP; via amide nitrogen and carbonyl oxygen By similarity

Site

179

Critical for catalysis By similarity

Site

251

Critical for catalysis By similarity

Amino acid modifications

Modified residue

106

N6-acetyllysine By similarity

Modified residue

272

N6-acetyllysine By similarity

Experimental info

Sequence conflict

P → K AA sequence Ref.2

Sequence conflict

E → V AA sequence Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P56574-1 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: 2466E1CC2C2CE9D1
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FASTA

452

50,967

        10         20         30         40         50         60 
MAGYLRAVSS LCRASGSTRT WAPAALNVPS WPEQPRRHYA EKRIKVEKPV VEMDGDEMTR 

        70         80         90        100        110        120 
IIWQFIKEKL ILPHVDVQLK YFDLGLPNRD QTNDQVTIDS ALATQKYSVA VKCATITPDE 

       130        140        150        160        170        180 
ARVEEFKLKK MWKSPNGTIR NILGGTVFRE PIICKNIPRL VPGWTKPITI GRHAHGDQYK 

       190        200        210        220        230        240 
ATDFVVDRAG MFKLVFTPKD GSGAKEWEVY NFPAGGVGMG MYNTDESISG FAHSCFQYSI 

       250        260        270        280        290        300 
QKKWPLYLST KNTIMKAYDG RFKDIFQEIF DKHYKTDFDK NKIWYEHRLI DDMVAQVLKS 

       310        320        330        340        350        360 
SGGFVWACKN YDGDVQSDIL AQGFGSLGLM TSVLVCPDGK TIEAEAAHGT VTRHYREHQK 

       370        380        390        400        410        420 
GRPTSTNPIA SIFAWTRGLE HRGKLDGNQD LIRFAQTLEK VCVQTVESGA MTKDLAGCIH 

       430        440        450 
GLSNVKLNEH FLNTTDFLDT IKSNLDRALG KQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[2]	Li X.-P., Pleissner K.-P., Scheler C., Regitz-Zagrosek V., Salikov J., Jungblut P.R. Submitted (SEP-1998) to UniProtKB Cited for: PROTEIN SEQUENCE OF 40-52, SUBCELLULAR LOCATION. Strain: Wistar. Tissue: Heart.
[3]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 61-67; 70-89; 113-122; 141-149; 181-188; 244-251; 262-272; 289-299; 341-353 AND 414-426, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.

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Cross-references

Sequence databases
	BC076398 mRNA. Translation: AAH76398.1.
RefSeq	NP_001014183.1.
UniGene	Rn.3490
3D structure databases
SMR	P56574. Positions 40-452.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000013949. Rattus norvegicus. [Contig view]
GeneID	361596.
KEGG	rno:361596.
Organism-specific databases
RGD	1597139. Idh2.
Phylogenomic databases
HOVERGEN	P56574.
Gene expression databases
ArrayExpress	P56574.
Family and domain databases
InterPro	IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	676849.

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Entry information

Entry name

IDHP_RAT

Accession

Primary (citable) accession number: P56574
Secondary accession number(s): Q6DGF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 12, 2006
Last modified:	November 25, 2008
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents