Reviewed, UniProtKB/Swiss-Prot P56568 (RIP1_LUFCY)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein luffin P1     EC=3.2.2.22 Alternative name(s):     Arginine/glutamate-rich polypeptide       Short name=AGRP Cleaved into the following 3 chains:     1- Recommended name:             Luffin P1a     2- Recommended name:             Luffin P1b     3- Recommended name:             Luffin P1c
Organism	Luffa cylindrica (Smooth loofah) (Sponge gourd)
Taxonomic identifier	3670 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Luffa

Protein attributes

Sequence length	47 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits protein synthesis in animal cells. Ref.2
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.2
Subunit structure	Homotetramer.
Mass spectrometry	Molecular mass is 5693.39 Da from positions 1 - 47. Determined by MALDI. Ref.1 Molecular mass is 5226.1 Da from positions 3 - 45. Determined by MALDI. Ref.2

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Glycosidase Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Ref.2 Inferred from direct assay. Source: UniProtKB metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Ref.2 Inferred from direct assay. Source: UniProtKB pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 47	47	Luffin P1a		PRO_0000032204
	Peptide	1 – 45	45	Luffin P1b		PRO_0000032205
	Peptide	3 – 45	43	Luffin P1c		PRO_0000032206
Sites
	Site	2 – 3	2	Cleavage Probable
	Site	45 – 46	2	Cleavage Probable
Amino acid modifications
	Disulfide bond	12 ↔ 33
	Disulfide bond	16 ↔ 29

Sequences

	Sequence		Length	Mass (Da)
	P56568-1 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 588B0EC82273AC05	FASTA	47	5,698
	10 20 30 40 PRGSPRTEYE ACRVRCQVAE HGVERQRRCQ QVCEKRLRER EGRREVD

References

[1]	"Primary structure of 6.5k-arginine/glutamate-rich polypeptide from the seeds of sponge gourd (Luffa cylindrica)." Kimura M., Park S.-S., Sakai R., Yamasaki N., Funatsu G. Biosci. Biotechnol. Biochem. 61:984-988(1997) [PubMed: 9214759] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Seed.
[2]	"Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica." Li F., Yang X.-X., Xia H.-C., Zeng R., Hu W.-G., Li Z., Zhang Z.-C. Peptides 24:799-805(2003) [PubMed: 12948831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-45, PROTEIN SEQUENCE OF 3-13, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF537345 mRNA. Translation: AAN06821.1. AY135368 mRNA. Translation: AAN10153.1.
PIR	JC5557.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273560.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

RIP1_LUFCY

Accession

Primary (citable) accession number: P56568
Secondary accession number(s): Q8GRS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)