P56568 (RIP1_LUFCY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribosome-inactivating protein luffin P1 EC=3.2.2.22 Alternative name(s): Arginine/glutamate-rich polypeptide Short name=AGRP Cleaved into the following 3 chains: Luffin P1a Luffin P1b Luffin P1c
Organism	Luffa cylindrica (Smooth loofah) (Sponge gourd)
Taxonomic identifier	3670 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Sicyoeae › Luffa

Protein attributes

Sequence length	47 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits protein synthesis in animal cells. Ref.2
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.2
Subunit structure	Homotetramer.
Mass spectrometry	Molecular mass is 5693.39 Da from positions 1 - 47. Determined by MALDI. Ref.1 Molecular mass is 5226.1 Da from positions 3 - 45. Determined by MALDI. Ref.2

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Glycosidase Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from direct assay Ref.2. Source: UniProtKB negative regulation of translation Inferred from direct assay Ref.2. Source: UniProtKB
Molecular function	rRNA N-glycosylase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 47

47

Luffin P1a

PRO_0000032204

Peptide

1 – 45

45

Luffin P1b

PRO_0000032205

Peptide

3 – 45

43

Luffin P1c

PRO_0000032206

Sites

Site

2 – 3

2

Cleavage Probable

Site

45 – 46

2

Cleavage Probable

Amino acid modifications

Disulfide bond

12 ↔ 33

Disulfide bond

16 ↔ 29

Secondary structure

1

.

.

.

.

.

47

Helix Strand Turn

Details...

Helix

6 – 19

14

Helix

24 – 40

17

Sequences

	Sequence		Length	Mass (Da)
	P56568 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 588B0EC82273AC05	FASTA	47	5,698
	10 20 30 40 PRGSPRTEYE ACRVRCQVAE HGVERQRRCQ QVCEKRLRER EGRREVD

References

[1]	"Primary structure of 6.5k-arginine/glutamate-rich polypeptide from the seeds of sponge gourd (Luffa cylindrica)." Kimura M., Park S.-S., Sakai R., Yamasaki N., Funatsu G. Biosci. Biotechnol. Biochem. 61:984-988(1997) [PubMed: 9214759] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Seed.
[2]	"Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica." Li F., Yang X.-X., Xia H.-C., Zeng R., Hu W.-G., Li Z., Zhang Z.-C. Peptides 24:799-805(2003) [PubMed: 12948831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-45, PROTEIN SEQUENCE OF 3-13, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY. Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF537345 mRNA. Translation: AAN06821.1.
AY135368 mRNA. Translation: AAN10153.1.

PIR

JC5557.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L37	NMR	-	A	3-45	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

ProtoNet

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Entry information

Entry name

RIP1_LUFCY

Accession

Primary (citable) accession number: P56568
Secondary accession number(s): Q8GRS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	June 28, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program