Reviewed,
UniProtKB/Swiss-Prot P56568 (RIP1_LUFCY)
Last modified
June 16, 2009.
Version 37.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information
Names and origin
| Protein names | Recommended name: Ribosome-inactivating protein luffin P1 EC=3.2.2.22 Alternative name(s): Arginine/glutamate-rich polypeptide Short name=AGRP Cleaved into the following 3 chains: 1- Recommended name: Luffin P1a 2- Recommended name: Luffin P1b 3- Recommended name: Luffin P1c |
| Organism | Luffa cylindrica (Smooth loofah) (Sponge gourd) |
| Taxonomic identifier | 3670 [NCBI] |
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Luffa |
Protein attributes
| Sequence length | 47 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Inhibits protein synthesis in animal cells. Ref.2 |
| Catalytic activity | Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.2 |
| Subunit structure | Homotetramer. |
| Mass spectrometry | Molecular mass is 5693.39 Da from positions 1 - 47. Determined by MALDI. Ref.1 Molecular mass is 5226.1 Da from positions 3 - 45. Determined by MALDI. Ref.2 |
Ontologies
| Keywords | |
|---|---|
| Biological process | Plant defense |
| Molecular function | Glycosidase Hydrolase Protein synthesis inhibitor Toxin |
| PTM | Disulfide bond |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | defense response Ref.2 Inferred from direct assay. Source: UniProtKB metabolic processInferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Ref.2Inferred from direct assay. Source: UniProtKB pathogenesisInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | rRNA N-glycosylase activity Ref.2 Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Peptide | 1 – 47 | 47 | Luffin P1a | PRO_0000032204 | |||||||
| Peptide | 1 – 45 | 45 | Luffin P1b | PRO_0000032205 | |||||||
| Peptide | 3 – 45 | 43 | Luffin P1c | PRO_0000032206 | |||||||
Sites | |||||||||||
| Site | 2 – 3 | 2 | Cleavage Probable | ||||||||
| Site | 45 – 46 | 2 | Cleavage Probable | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 12 ↔ 33 | ||||||||||
| Disulfide bond | 16 ↔ 29 | ||||||||||
Sequences
References
| [1] | "Primary structure of 6.5k-arginine/glutamate-rich polypeptide from the seeds of sponge gourd (Luffa cylindrica)." Kimura M., Park S.-S., Sakai R., Yamasaki N., Funatsu G. Biosci. Biotechnol. Biochem. 61:984-988(1997) [PubMed: 9214759] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Seed. |
| [2] | "Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica." Li F., Yang X.-X., Xia H.-C., Zeng R., Hu W.-G., Li Z., Zhang Z.-C. Peptides 24:799-805(2003) [PubMed: 12948831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-45, PROTEIN SEQUENCE OF 3-13, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY. Tissue: Seed. |
Cross-references
Sequence databases | |
|---|---|
| AF537345 mRNA. Translation: AAN06821.1. AY135368 mRNA. Translation: AAN10153.1. | |
| PIR | JC5557. |
3D structure databases | |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 3.2.2.22. 273560. |
Family and domain databases | |
| ProtoNet | Search... |
Entry information
| Entry name | RIP1_LUFCY | ||||||||
| Accession | Primary (citable) accession number: P56568 Secondary accession number(s): Q8GRS9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||

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