P56564 (EAA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Excitatory amino acid transporter 1

Alternative name(s):
Glial high affinity glutamate transporter
High-affinity neuronal glutamate transporter
Sodium-dependent glutamate/aspartate transporter 1
Short name=GLAST-1
Solute carrier family 1 member 3

Gene names

Name:	Slc1a3
Synonyms:	Eaat1, Gmt1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum. Intermediate level in lung, low level in spleen, skeletal muscle and testis.
Sequence similarities	Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	D-aspartate import Inferred from electronic annotation. Source: Compara L-glutamate import Inferred from mutant phenotype PubMed 15390100. Source: MGI auditory behavior Inferred from mutant phenotype PubMed 11102482. Source: MGI cell morphogenesis involved in neuron differentiation Inferred from mutant phenotype PubMed 9753165. Source: MGI cranial nerve development Inferred from mutant phenotype PubMed 9753165. Source: MGI gamma-aminobutyric acid biosynthetic process Inferred from mutant phenotype PubMed 15305132. Source: MGI glutamate biosynthetic process Inferred from mutant phenotype PubMed 15305132. Source: MGI neuromuscular process controlling balance Inferred from mutant phenotype PubMed 9753165. Source: MGI positive regulation of synaptic transmission Inferred from mutant phenotype PubMed 9539795. Source: MGI response to amino acid stimulus Inferred from sequence orthology PubMed 7723632. Source: MGI response to antibiotic Inferred from mutant phenotype PubMed 15862894. Source: MGI response to drug Inferred from mutant phenotype PubMed 9539795. Source: MGI response to inorganic substance Inferred from sequence orthology PubMed 7723632. Source: MGI response to light stimulus Inferred from mutant phenotype PubMed 9539795. Source: MGI response to wounding Inferred from mutant phenotype PubMed 9753165. Source: MGI sensory perception of sound Inferred from mutant phenotype PubMed 11102482. Source: MGI
Cellular_component	cell projection Inferred from direct assay PubMed 12971893PubMed 14723703PubMed 15965470. Source: MGI cell surface Inferred from direct assay PubMed 14723703. Source: MGI fibril Inferred from direct assay PubMed 9671661. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 11133151PubMed 12971893PubMed 15965470PubMed 9364068. Source: MGI plasma membrane Inferred from direct assay PubMed 14723703. Source: MGI
Molecular_function	glutamate binding Inferred from direct assay Ref.1. Source: MGI high-affinity glutamate transmembrane transporter activity Inferred from direct assay PubMed 14723703Ref.1. Source: MGI sodium:dicarboxylate symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Excitatory amino acid transporter 1		PRO_0000202058
Regions
	Topological domain	1 – 47	47	Cytoplasmic Potential
	Transmembrane	48 – 68	21	Helical; Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Transmembrane	123 – 145	23	Helical; Potential
	Topological domain	146 – 237	92	Extracellular Potential
	Transmembrane	238 – 260	23	Helical; Potential
	Transmembrane	281 – 302	22	Helical; Potential
	Transmembrane	319 – 340	22	Helical; Potential
	Compositional bias	363 – 366	4	Poly-Ser
Amino acid modifications
	Modified residue	512	1	Phosphoserine Ref.4
	Glycosylation	206	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	215	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	216	1	N-linked (GlcNAc...) Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P56564 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: E0B24CBA1D5B086D	FASTA	543	59,622
	10 20 30 40 50 60 MTKSNGEEPR MGGRMERLQQ GVRKRTLLAK KKVQSLTKED VKSYLFRNAF VLLTVTAVIV 70 80 90 100 110 120 GTILGFALRP YKMSYREVKY FSFPGELLMR MLQMLVLPLI ISSLVTGMAA LDSKASGKMG 130 140 150 160 170 180 MRAVVYYMTT TIIAVVIGII IVIIIHPGKG TKENMYREGK IVQVTAADAF LDLIRNMFPP 190 200 210 220 230 240 NLVEACFKQF KTSYEKRSFK VPIQSNETLL GAVINNVSEA METLTRIREE MVPVPGSVNG 250 260 270 280 290 300 VNALGLVVFS MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK 310 320 330 340 350 360 IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG GLLQALITAL 370 380 390 400 410 420 GTSSSSATLP ITFKCLEENN GVDKRITRFV LPVGATINMD GTALYEALAA IFIAQVNNFD 430 440 450 460 470 480 LNFGQIITIS ITATAASIGA AGIPQAGLVT MVIVLTSVGL PTDDITLIIA VDWFLDRLRT 490 500 510 520 530 540 TTNVLGDSLG AGIVEHLSRH ELKNRDVEMG NSVIEENEMK KPYQLIAQDN EPEKPVADSE TKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a glutamate transporter from mouse brain." Tanaka K. Neurosci. Lett. 159:183-186(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain and Neural stem cell.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 80-114; 161-175; 189-196; 269-280 AND 480-499, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, MASS SPECTROMETRY. Tissue: Brain cortex.
[5]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206; ASN-215 AND ASN-216, MASS SPECTROMETRY. Tissue: Myoblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF330257 mRNA. Translation: AAK01708.1. BC058711 mRNA. Translation: AAH58711.1. BC066154 mRNA. Translation: AAH66154.1.
IPI	IPI00114279.
RefSeq	NP_683740.1. NM_148938.3.
UniGene	Mm.204834.
3D structure databases
ProteinModelPortal	P56564.
SMR	P56564. Positions 59-494.
ModBase	Search...
Protein-protein interaction databases
IntAct	P56564. 2 interactions.
PTM databases
PhosphoSite	P56564.
Proteomic databases
PaxDb	P56564.
PRIDE	P56564.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000005493; ENSMUSP00000005493; ENSMUSG00000005360.
GeneID	20512.
KEGG	mmu:20512.
Organism-specific databases
CTD	6507.
MGI	MGI:99917. Slc1a3.
Phylogenomic databases
eggNOG	COG1301.
HOGENOM	HOG000208776.
HOVERGEN	HBG000080.
InParanoid	P56564.
KO	K05614.
OMA	TLRPYRM.
OrthoDB	EOG46HG9R.
Gene expression databases
ArrayExpress	P56564.
Bgee	P56564.
Genevestigator	P56564.
GermOnline	ENSMUSG00000005360. Mus musculus.
Family and domain databases
InterPro	IPR001991. Na-dicarboxylate_symporter. IPR018107. Na-dicarboxylate_symporter_CS. [Graphical view]
PANTHER	PTHR11958. PTHR11958. 1 hit.
Pfam	PF00375. SDF. 1 hit. [Graphical view]
PRINTS	PR00173. EDTRNSPORT.
PROSITE	PS00713. NA_DICARBOXYL_SYMP_1. 1 hit. PS00714. NA_DICARBOXYL_SYMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	298711.
SOURCE	Search...

Entry information

Entry name

EAA1_MOUSE

Accession

Primary (citable) accession number: P56564
Secondary accession number(s): Q99P53

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	March 5, 2002
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program