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P56564

- EAA1_MOUSE

UniProt

P56564 - EAA1_MOUSE

Protein

Excitatory amino acid transporter 1

Gene

Slc1a3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (05 Mar 2002)
      Previous versions | rss
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    Functioni

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

    GO - Molecular functioni

    1. amino acid binding Source: MGI
    2. glutamate binding Source: MGI
    3. high-affinity glutamate transmembrane transporter activity Source: MGI
    4. protein binding Source: MGI
    5. sodium:dicarboxylate symporter activity Source: InterPro

    GO - Biological processi

    1. auditory behavior Source: MGI
    2. cell morphogenesis involved in neuron differentiation Source: MGI
    3. cranial nerve development Source: MGI
    4. D-aspartate import Source: Ensembl
    5. gamma-aminobutyric acid biosynthetic process Source: MGI
    6. glutamate biosynthetic process Source: MGI
    7. glutamate metabolic process Source: MGI
    8. L-glutamate import Source: MGI
    9. L-glutamate transport Source: MGI
    10. neuromuscular process controlling balance Source: MGI
    11. positive regulation of synaptic transmission Source: MGI
    12. response to amino acid Source: MGI
    13. response to antibiotic Source: MGI
    14. response to drug Source: MGI
    15. response to inorganic substance Source: MGI
    16. response to light stimulus Source: MGI
    17. response to wounding Source: MGI
    18. sensory perception of sound Source: MGI

    Keywords - Biological processi

    Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Excitatory amino acid transporter 1
    Alternative name(s):
    Glial high affinity glutamate transporter
    High-affinity neuronal glutamate transporter
    Sodium-dependent glutamate/aspartate transporter 1
    Short name:
    GLAST-1
    Solute carrier family 1 member 3
    Gene namesi
    Name:Slc1a3
    Synonyms:Eaat1, Gmt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:99917. Slc1a3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell projection Source: MGI
    2. cell surface Source: MGI
    3. fibril Source: MGI
    4. integral component of membrane Source: UniProtKB-KW
    5. neuronal cell body Source: MGI
    6. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 543543Excitatory amino acid transporter 1PRO_0000202058Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi206 – 2061N-linked (GlcNAc...)1 Publication
    Glycosylationi215 – 2151N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi216 – 2161N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP56564.
    PaxDbiP56564.
    PRIDEiP56564.

    PTM databases

    PhosphoSiteiP56564.

    Expressioni

    Tissue specificityi

    Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum. Intermediate level in lung, low level in spleen, skeletal muscle and testis.

    Gene expression databases

    ArrayExpressiP56564.
    BgeeiP56564.
    GenevestigatoriP56564.

    Interactioni

    Protein-protein interaction databases

    BioGridi203291. 3 interactions.
    IntActiP56564. 2 interactions.
    MINTiMINT-4996875.

    Structurei

    3D structure databases

    ProteinModelPortaliP56564.
    SMRiP56564. Positions 59-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4747CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini146 – 23792ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei48 – 6821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei91 – 11121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei238 – 26023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei281 – 30222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 34022HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi363 – 3664Poly-Ser

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1301.
    HOGENOMiHOG000208776.
    HOVERGENiHBG000080.
    InParanoidiP56564.
    KOiK05614.
    OMAiTLRPYRM.
    OrthoDBiEOG7RV9G2.
    PhylomeDBiP56564.
    TreeFamiTF315206.

    Family and domain databases

    Gene3Di1.10.3860.10. 2 hits.
    InterProiIPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11958. PTHR11958. 1 hit.
    PfamiPF00375. SDF. 1 hit.
    [Graphical view]
    PRINTSiPR00173. EDTRNSPORT.
    SUPFAMiSSF118215. SSF118215. 2 hits.
    PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56564-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKSNGEEPR MGGRMERLQQ GVRKRTLLAK KKVQSLTKED VKSYLFRNAF    50
    VLLTVTAVIV GTILGFALRP YKMSYREVKY FSFPGELLMR MLQMLVLPLI 100
    ISSLVTGMAA LDSKASGKMG MRAVVYYMTT TIIAVVIGII IVIIIHPGKG 150
    TKENMYREGK IVQVTAADAF LDLIRNMFPP NLVEACFKQF KTSYEKRSFK 200
    VPIQSNETLL GAVINNVSEA METLTRIREE MVPVPGSVNG VNALGLVVFS 250
    MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK 300
    IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG 350
    GLLQALITAL GTSSSSATLP ITFKCLEENN GVDKRITRFV LPVGATINMD 400
    GTALYEALAA IFIAQVNNFD LNFGQIITIS ITATAASIGA AGIPQAGLVT 450
    MVIVLTSVGL PTDDITLIIA VDWFLDRLRT TTNVLGDSLG AGIVEHLSRH 500
    ELKNRDVEMG NSVIEENEMK KPYQLIAQDN EPEKPVADSE TKM 543
    Length:543
    Mass (Da):59,622
    Last modified:March 5, 2002 - v2
    Checksum:iE0B24CBA1D5B086D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF330257 mRNA. Translation: AAK01708.1.
    BC058711 mRNA. Translation: AAH58711.1.
    BC066154 mRNA. Translation: AAH66154.1.
    CCDSiCCDS27373.1.
    RefSeqiNP_683740.1. NM_148938.3.
    UniGeneiMm.204834.

    Genome annotation databases

    EnsembliENSMUST00000005493; ENSMUSP00000005493; ENSMUSG00000005360.
    GeneIDi20512.
    KEGGimmu:20512.
    UCSCiuc007vex.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF330257 mRNA. Translation: AAK01708.1 .
    BC058711 mRNA. Translation: AAH58711.1 .
    BC066154 mRNA. Translation: AAH66154.1 .
    CCDSi CCDS27373.1.
    RefSeqi NP_683740.1. NM_148938.3.
    UniGenei Mm.204834.

    3D structure databases

    ProteinModelPortali P56564.
    SMRi P56564. Positions 59-494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203291. 3 interactions.
    IntActi P56564. 2 interactions.
    MINTi MINT-4996875.

    PTM databases

    PhosphoSitei P56564.

    Proteomic databases

    MaxQBi P56564.
    PaxDbi P56564.
    PRIDEi P56564.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005493 ; ENSMUSP00000005493 ; ENSMUSG00000005360 .
    GeneIDi 20512.
    KEGGi mmu:20512.
    UCSCi uc007vex.1. mouse.

    Organism-specific databases

    CTDi 6507.
    MGIi MGI:99917. Slc1a3.

    Phylogenomic databases

    eggNOGi COG1301.
    HOGENOMi HOG000208776.
    HOVERGENi HBG000080.
    InParanoidi P56564.
    KOi K05614.
    OMAi TLRPYRM.
    OrthoDBi EOG7RV9G2.
    PhylomeDBi P56564.
    TreeFami TF315206.

    Miscellaneous databases

    NextBioi 298711.
    PROi P56564.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P56564.
    Bgeei P56564.
    Genevestigatori P56564.

    Family and domain databases

    Gene3Di 1.10.3860.10. 2 hits.
    InterProi IPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view ]
    PANTHERi PTHR11958. PTHR11958. 1 hit.
    Pfami PF00375. SDF. 1 hit.
    [Graphical view ]
    PRINTSi PR00173. EDTRNSPORT.
    SUPFAMi SSF118215. SSF118215. 2 hits.
    PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a glutamate transporter from mouse brain."
      Tanaka K.
      Neurosci. Lett. 159:183-186(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Neural stem cell.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 80-114; 161-175; 189-196; 269-280 AND 480-499, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    5. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206; ASN-215 AND ASN-216.
      Tissue: Myoblast.

    Entry informationi

    Entry nameiEAA1_MOUSE
    AccessioniPrimary (citable) accession number: P56564
    Secondary accession number(s): Q99P53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: March 5, 2002
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3