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P56564

- EAA1_MOUSE

UniProt

P56564 - EAA1_MOUSE

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Protein
Excitatory amino acid transporter 1
Gene
Slc1a3, Eaat1, Gmt1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

GO - Molecular functioni

  1. amino acid binding Source: MGI
  2. glutamate binding Source: MGI
  3. high-affinity glutamate transmembrane transporter activity Source: MGI
  4. protein binding Source: MGI
  5. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. D-aspartate import Source: Ensembl
  2. L-glutamate import Source: MGI
  3. L-glutamate transport Source: MGI
  4. auditory behavior Source: MGI
  5. cell morphogenesis involved in neuron differentiation Source: MGI
  6. cranial nerve development Source: MGI
  7. gamma-aminobutyric acid biosynthetic process Source: MGI
  8. glutamate biosynthetic process Source: MGI
  9. glutamate metabolic process Source: MGI
  10. neuromuscular process controlling balance Source: MGI
  11. positive regulation of synaptic transmission Source: MGI
  12. response to amino acid Source: MGI
  13. response to antibiotic Source: MGI
  14. response to drug Source: MGI
  15. response to inorganic substance Source: MGI
  16. response to light stimulus Source: MGI
  17. response to wounding Source: MGI
  18. sensory perception of sound Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 1
Alternative name(s):
Glial high affinity glutamate transporter
High-affinity neuronal glutamate transporter
Sodium-dependent glutamate/aspartate transporter 1
Short name:
GLAST-1
Solute carrier family 1 member 3
Gene namesi
Name:Slc1a3
Synonyms:Eaat1, Gmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:99917. Slc1a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei48 – 6821Helical; Reviewed prediction
Add
BLAST
Transmembranei91 – 11121Helical; Reviewed prediction
Add
BLAST
Transmembranei123 – 14523Helical; Reviewed prediction
Add
BLAST
Topological domaini146 – 23792Extracellular Reviewed prediction
Add
BLAST
Transmembranei238 – 26023Helical; Reviewed prediction
Add
BLAST
Transmembranei281 – 30222Helical; Reviewed prediction
Add
BLAST
Transmembranei319 – 34022Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell projection Source: MGI
  2. cell surface Source: MGI
  3. fibril Source: MGI
  4. integral component of membrane Source: UniProtKB-KW
  5. neuronal cell body Source: MGI
  6. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Excitatory amino acid transporter 1
PRO_0000202058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi206 – 2061N-linked (GlcNAc...)1 Publication
Glycosylationi215 – 2151N-linked (GlcNAc...); atypical1 Publication
Glycosylationi216 – 2161N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP56564.
PaxDbiP56564.
PRIDEiP56564.

PTM databases

PhosphoSiteiP56564.

Expressioni

Tissue specificityi

Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum. Intermediate level in lung, low level in spleen, skeletal muscle and testis.

Gene expression databases

ArrayExpressiP56564.
BgeeiP56564.
GenevestigatoriP56564.

Interactioni

Protein-protein interaction databases

BioGridi203291. 3 interactions.
IntActiP56564. 2 interactions.
MINTiMINT-4996875.

Structurei

3D structure databases

ProteinModelPortaliP56564.
SMRiP56564. Positions 59-494.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi363 – 3664Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP56564.
KOiK05614.
OMAiTLRPYRM.
OrthoDBiEOG7RV9G2.
PhylomeDBiP56564.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56564-1 [UniParc]FASTAAdd to Basket

« Hide

MTKSNGEEPR MGGRMERLQQ GVRKRTLLAK KKVQSLTKED VKSYLFRNAF    50
VLLTVTAVIV GTILGFALRP YKMSYREVKY FSFPGELLMR MLQMLVLPLI 100
ISSLVTGMAA LDSKASGKMG MRAVVYYMTT TIIAVVIGII IVIIIHPGKG 150
TKENMYREGK IVQVTAADAF LDLIRNMFPP NLVEACFKQF KTSYEKRSFK 200
VPIQSNETLL GAVINNVSEA METLTRIREE MVPVPGSVNG VNALGLVVFS 250
MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK 300
IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG 350
GLLQALITAL GTSSSSATLP ITFKCLEENN GVDKRITRFV LPVGATINMD 400
GTALYEALAA IFIAQVNNFD LNFGQIITIS ITATAASIGA AGIPQAGLVT 450
MVIVLTSVGL PTDDITLIIA VDWFLDRLRT TTNVLGDSLG AGIVEHLSRH 500
ELKNRDVEMG NSVIEENEMK KPYQLIAQDN EPEKPVADSE TKM 543
Length:543
Mass (Da):59,622
Last modified:March 5, 2002 - v2
Checksum:iE0B24CBA1D5B086D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF330257 mRNA. Translation: AAK01708.1.
BC058711 mRNA. Translation: AAH58711.1.
BC066154 mRNA. Translation: AAH66154.1.
CCDSiCCDS27373.1.
RefSeqiNP_683740.1. NM_148938.3.
UniGeneiMm.204834.

Genome annotation databases

EnsembliENSMUST00000005493; ENSMUSP00000005493; ENSMUSG00000005360.
GeneIDi20512.
KEGGimmu:20512.
UCSCiuc007vex.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF330257 mRNA. Translation: AAK01708.1 .
BC058711 mRNA. Translation: AAH58711.1 .
BC066154 mRNA. Translation: AAH66154.1 .
CCDSi CCDS27373.1.
RefSeqi NP_683740.1. NM_148938.3.
UniGenei Mm.204834.

3D structure databases

ProteinModelPortali P56564.
SMRi P56564. Positions 59-494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203291. 3 interactions.
IntActi P56564. 2 interactions.
MINTi MINT-4996875.

PTM databases

PhosphoSitei P56564.

Proteomic databases

MaxQBi P56564.
PaxDbi P56564.
PRIDEi P56564.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005493 ; ENSMUSP00000005493 ; ENSMUSG00000005360 .
GeneIDi 20512.
KEGGi mmu:20512.
UCSCi uc007vex.1. mouse.

Organism-specific databases

CTDi 6507.
MGIi MGI:99917. Slc1a3.

Phylogenomic databases

eggNOGi COG1301.
HOGENOMi HOG000208776.
HOVERGENi HBG000080.
InParanoidi P56564.
KOi K05614.
OMAi TLRPYRM.
OrthoDBi EOG7RV9G2.
PhylomeDBi P56564.
TreeFami TF315206.

Miscellaneous databases

NextBioi 298711.
PROi P56564.
SOURCEi Search...

Gene expression databases

ArrayExpressi P56564.
Bgeei P56564.
Genevestigatori P56564.

Family and domain databases

Gene3Di 1.10.3860.10. 2 hits.
InterProi IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view ]
PANTHERi PTHR11958. PTHR11958. 1 hit.
Pfami PF00375. SDF. 1 hit.
[Graphical view ]
PRINTSi PR00173. EDTRNSPORT.
SUPFAMi SSF118215. SSF118215. 2 hits.
PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a glutamate transporter from mouse brain."
    Tanaka K.
    Neurosci. Lett. 159:183-186(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Neural stem cell.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 80-114; 161-175; 189-196; 269-280 AND 480-499, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  5. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206; ASN-215 AND ASN-216.
    Tissue: Myoblast.

Entry informationi

Entry nameiEAA1_MOUSE
AccessioniPrimary (citable) accession number: P56564
Secondary accession number(s): Q99P53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 5, 2002
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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