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P56564 (EAA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Excitatory amino acid transporter 1
Alternative name(s):
Glial high affinity glutamate transporter
High-affinity neuronal glutamate transporter
Sodium-dependent glutamate/aspartate transporter 1
Short name=GLAST-1
Solute carrier family 1 member 3
Gene names
Name:Slc1a3
Synonyms:Eaat1, Gmt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum. Intermediate level in lung, low level in spleen, skeletal muscle and testis.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. [View classification]

Ontologies

Keywords
   Biological processSymport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processD-aspartate import

Inferred from electronic annotation. Source: Ensembl

L-glutamate import

Inferred from mutant phenotype PubMed 15390100. Source: MGI

L-glutamate transport

Inferred from direct assay PubMed 14723703. Source: MGI

auditory behavior

Inferred from mutant phenotype PubMed 11102482. Source: MGI

cell morphogenesis involved in neuron differentiation

Inferred from mutant phenotype PubMed 9753165. Source: MGI

cranial nerve development

Inferred from mutant phenotype PubMed 9753165. Source: MGI

gamma-aminobutyric acid biosynthetic process

Inferred from mutant phenotype PubMed 15305132. Source: MGI

glutamate biosynthetic process

Inferred from mutant phenotype PubMed 15305132. Source: MGI

glutamate metabolic process

Inferred from mutant phenotype PubMed 11102482. Source: MGI

neuromuscular process controlling balance

Inferred from mutant phenotype PubMed 9753165. Source: MGI

positive regulation of synaptic transmission

Inferred from mutant phenotype PubMed 9539795. Source: MGI

response to amino acid

Inferred from sequence orthology PubMed 7723632. Source: MGI

response to antibiotic

Inferred from mutant phenotype PubMed 15862894. Source: MGI

response to drug

Inferred from mutant phenotype PubMed 9539795. Source: MGI

response to inorganic substance

Inferred from sequence orthology PubMed 7723632. Source: MGI

response to light stimulus

Inferred from mutant phenotype PubMed 9539795. Source: MGI

response to wounding

Inferred from mutant phenotype PubMed 9753165. Source: MGI

sensory perception of sound

Inferred from mutant phenotype PubMed 11102482. Source: MGI

   Cellular_componentcell projection

Inferred from direct assay PubMed 12971893PubMed 14723703PubMed 15965470. Source: MGI

cell surface

Inferred from direct assay PubMed 14723703. Source: MGI

fibril

Inferred from direct assay PubMed 9671661. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from direct assay PubMed 11133151PubMed 12971893PubMed 15965470PubMed 9364068. Source: MGI

plasma membrane

Inferred from direct assay PubMed 14723703. Source: MGI

   Molecular_functionamino acid binding

Inferred from direct assay Ref.1. Source: MGI

glutamate binding

Inferred from direct assay Ref.1. Source: MGI

high-affinity glutamate transmembrane transporter activity

Inferred from direct assay PubMed 14723703Ref.1. Source: MGI

sodium:dicarboxylate symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 543543Excitatory amino acid transporter 1
PRO_0000202058

Regions

Topological domain1 – 4747Cytoplasmic Potential
Transmembrane48 – 6821Helical; Potential
Transmembrane91 – 11121Helical; Potential
Transmembrane123 – 14523Helical; Potential
Topological domain146 – 23792Extracellular Potential
Transmembrane238 – 26023Helical; Potential
Transmembrane281 – 30222Helical; Potential
Transmembrane319 – 34022Helical; Potential
Compositional bias363 – 3664Poly-Ser

Amino acid modifications

Glycosylation2061N-linked (GlcNAc...) Ref.5
Glycosylation2151N-linked (GlcNAc...); atypical Ref.5
Glycosylation2161N-linked (GlcNAc...) Ref.5

Sequences

Sequence LengthMass (Da)Tools
P56564 [UniParc].

Last modified March 5, 2002. Version 2.
Checksum: E0B24CBA1D5B086D

FASTA54359,622
        10         20         30         40         50         60 
MTKSNGEEPR MGGRMERLQQ GVRKRTLLAK KKVQSLTKED VKSYLFRNAF VLLTVTAVIV 

        70         80         90        100        110        120 
GTILGFALRP YKMSYREVKY FSFPGELLMR MLQMLVLPLI ISSLVTGMAA LDSKASGKMG 

       130        140        150        160        170        180 
MRAVVYYMTT TIIAVVIGII IVIIIHPGKG TKENMYREGK IVQVTAADAF LDLIRNMFPP 

       190        200        210        220        230        240 
NLVEACFKQF KTSYEKRSFK VPIQSNETLL GAVINNVSEA METLTRIREE MVPVPGSVNG 

       250        260        270        280        290        300 
VNALGLVVFS MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP LGILFLIAGK 

       310        320        330        340        350        360 
IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG GLLQALITAL 

       370        380        390        400        410        420 
GTSSSSATLP ITFKCLEENN GVDKRITRFV LPVGATINMD GTALYEALAA IFIAQVNNFD 

       430        440        450        460        470        480 
LNFGQIITIS ITATAASIGA AGIPQAGLVT MVIVLTSVGL PTDDITLIIA VDWFLDRLRT 

       490        500        510        520        530        540 
TTNVLGDSLG AGIVEHLSRH ELKNRDVEMG NSVIEENEMK KPYQLIAQDN EPEKPVADSE 


TKM 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a glutamate transporter from mouse brain."
Tanaka K.
Neurosci. Lett. 159:183-186(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Neural stem cell.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 80-114; 161-175; 189-196; 269-280 AND 480-499, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[4]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain cortex.
[5]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-206; ASN-215 AND ASN-216.
Tissue: Myoblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF330257 mRNA. Translation: AAK01708.1.
BC058711 mRNA. Translation: AAH58711.1.
BC066154 mRNA. Translation: AAH66154.1.
RefSeqNP_683740.1. NM_148938.3.
UniGeneMm.204834.

3D structure databases

ProteinModelPortalP56564.
SMRP56564. Positions 59-494.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203291. 3 interactions.
IntActP56564. 2 interactions.
MINTMINT-4996875.

PTM databases

PhosphoSiteP56564.

Proteomic databases

PaxDbP56564.
PRIDEP56564.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005493; ENSMUSP00000005493; ENSMUSG00000005360.
GeneID20512.
KEGGmmu:20512.
UCSCuc007vex.1. mouse.

Organism-specific databases

CTD6507.
MGIMGI:99917. Slc1a3.

Phylogenomic databases

eggNOGCOG1301.
HOGENOMHOG000208776.
HOVERGENHBG000080.
InParanoidP56564.
KOK05614.
OMATLRPYRM.
OrthoDBEOG7RV9G2.
PhylomeDBP56564.
TreeFamTF315206.

Gene expression databases

ArrayExpressP56564.
BgeeP56564.
GenevestigatorP56564.

Family and domain databases

Gene3D1.10.3860.10. 2 hits.
InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
SUPFAMSSF118215. SSF118215. 2 hits.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298711.
PROP56564.
SOURCESearch...

Entry information

Entry nameEAA1_MOUSE
AccessionPrimary (citable) accession number: P56564
Secondary accession number(s): Q99P53
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: March 5, 2002
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot