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Protein

Amine oxidase [flavin-containing] B

Gene

MAOB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine (By similarity).By similarity

Catalytic activityi

RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei156Important for catalytic activityBy similarity1
Sitei365Important for catalytic activityBy similarity1
Sitei382Important for catalytic activityBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.4.3.4. 908.

Names & Taxonomyi

Protein namesi
Recommended name:
Amine oxidase [flavin-containing] B (EC:1.4.3.4)
Alternative name(s):
Monoamine oxidase type B
Short name:
MAO-B
Gene namesi
Name:MAOB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 489CytoplasmicBy similarityAdd BLAST488
Transmembranei490 – 516Helical; Anchor for type IV membrane proteinBy similarityAdd BLAST27
Topological domaini517 – 520Mitochondrial intermembraneBy similarity4

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2756.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000998562 – 520Amine oxidase [flavin-containing] BAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei52N6-acetyllysineBy similarity1
Modified residuei397S-8alpha-FAD cysteineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP56560.
PeptideAtlasiP56560.
PRIDEiP56560.

Interactioni

Subunit structurei

Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000001698.

Chemistry databases

BindingDBiP56560.

Structurei

3D structure databases

ProteinModelPortaliP56560.
SMRiP56560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 52Arg/Lys-rich (basic)Add BLAST17

Sequence similaritiesi

Belongs to the flavin monoamine oxidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0029. Eukaryota.
ENOG410XSNC. LUCA.
HOGENOMiHOG000221615.
HOVERGENiHBG004255.
InParanoidiP56560.
KOiK00274.
TreeFamiTF313314.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiView protein in InterPro
IPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR001613. Flavin_amine_oxidase.
PfamiView protein in Pfam
PF01593. Amino_oxidase. 1 hit.
PRINTSiPR00757. AMINEOXDASEF.
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKCDVVVV GGGISGMAAA KLLHDSGLNV IVLEARDRVG GRTYTLRNQK
60 70 80 90 100
VKYVDLGGSY VGPTQNHILR LSKELGLETY KVNEVERLIH HTKGKSYPFR
110 120 130 140 150
GSFPSVWNPI TYLDHNNLWR TMDDMGREIP SDAPWKAPLA EQWDLMTMKE
160 170 180 190 200
LLDKICWTES SKQLAILFVN LCVTAEIHEV SALWFLWYVK QCGGTTRIFS
210 220 230 240 250
TSNGGQERKF VGGSGQVSER IMDLLGDRVK LERPVIHIDQ TGENVLVETL
260 270 280 290 300
NHELYEAKYV ISAVPPVLGM KIHFNPPLPM MRNQLITRVP LGSVIKSIVY
310 320 330 340 350
YKEPFWRNMD YCGSMIIEGE EAPVAYALDD TKPDGSYPAI IGFILAHKAR
360 370 380 390 400
KLARLTKEER LKKLCDLYAK VLGSQEALHP VHYEEKNWCE EQYSGGCYTS
410 420 430 440 450
YFPPGIMTQY GRVLRQPVGR IYFAGTETAT HWSGYMEGAV EAGERAAREI
460 470 480 490 500
LHAMGKIPED EIWLPEPESV DVPAKPITTT FLQRHLPSVP GLLKLIGLTT
510 520
IFSATALGFL AHKRGLLVRI
Length:520
Mass (Da):58,421
Last modified:January 23, 2007 - v4
Checksum:iA594889B4495C410
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3S → N in AAF23179 (Ref. 1) Curated1
Sequence conflicti270M → G AA sequence (PubMed:2719656).Curated1
Sequence conflicti286I → T in AAF23179 (Ref. 1) Curated1
Sequence conflicti297 – 299SIV → PIM AA sequence (PubMed:2719656).Curated3
Sequence conflicti307R → K AA sequence (PubMed:2719656).Curated1
Sequence conflicti341I → L AA sequence (PubMed:2719656).Curated1
Sequence conflicti344I → K AA sequence (PubMed:2719656).Curated1
Sequence conflicti400S → A AA sequence (PubMed:2719656).Curated1
Sequence conflicti478 – 485TTTFLQRH → STSSMMMP AA sequence (PubMed:2719656).Curated8
Sequence conflicti509F → Y in AAI19942 (Ref. 2) Curated1
Sequence conflicti520I → V in AAF23179 (Ref. 1) Curated1

Mass spectrometryi

Molecular mass is 59163.5±10.0 Da from positions 2 - 520. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217955 mRNA. Translation: AAF23179.1.
BC119941 mRNA. Translation: AAI19942.1.
PIRiS07573.
RefSeqiNP_808813.2. NM_177944.2.
UniGeneiBt.22460.

Genome annotation databases

GeneIDi338445.
KEGGibta:338445.

Similar proteinsi

Entry informationi

Entry nameiAOFB_BOVIN
AccessioniPrimary (citable) accession number: P56560
Secondary accession number(s): Q0P5K2, Q864W3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 123 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families