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P56560

- AOFB_BOVIN

UniProt

P56560 - AOFB_BOVIN

Protein

Amine oxidase [flavin-containing] B

Gene

MAOB

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine By similarity.By similarity

    Catalytic activityi

    RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.

    Cofactori

    FAD.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei156 – 1561Important for catalytic activityBy similarity
    Sitei365 – 3651Important for catalytic activityBy similarity
    Sitei382 – 3821Important for catalytic activityBy similarity

    GO - Molecular functioni

    1. oxidoreductase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BRENDAi1.4.3.4. 908.
    ReactomeiREACT_223515. Monoamines are oxidized to aldehydes by MAOA and MAOB, producing NH3 and H2O2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amine oxidase [flavin-containing] B (EC:1.4.3.4)
    Alternative name(s):
    Monoamine oxidase type B
    Short name:
    MAO-B
    Gene namesi
    Name:MAOB
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome X

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: Ensembl
    3. mitochondrial outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 520519Amine oxidase [flavin-containing] BPRO_0000099856Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei52 – 521N6-acetyllysineBy similarity
    Modified residuei397 – 3971S-8alpha-FAD cysteineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP56560.
    PRIDEiP56560.

    Interactioni

    Subunit structurei

    Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP56560.
    SMRiP56560. Positions 3-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 489488CytoplasmicBy similarityAdd
    BLAST
    Topological domaini517 – 5204Mitochondrial intermembraneBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei490 – 51627Helical; Anchor for type IV membrane proteinBy similarityAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi36 – 5217Arg/Lys-rich (basic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1231.
    GeneTreeiENSGT00730000110903.
    HOGENOMiHOG000221615.
    HOVERGENiHBG004255.
    InParanoidiP56560.
    KOiK00274.
    OrthoDBiEOG7K6PTP.
    TreeFamiTF313314.

    Family and domain databases

    InterProiIPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P56560-1 [UniParc]FASTAAdd to Basket

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    MSSKCDVVVV GGGISGMAAA KLLHDSGLNV IVLEARDRVG GRTYTLRNQK    50
    VKYVDLGGSY VGPTQNHILR LSKELGLETY KVNEVERLIH HTKGKSYPFR 100
    GSFPSVWNPI TYLDHNNLWR TMDDMGREIP SDAPWKAPLA EQWDLMTMKE 150
    LLDKICWTES SKQLAILFVN LCVTAEIHEV SALWFLWYVK QCGGTTRIFS 200
    TSNGGQERKF VGGSGQVSER IMDLLGDRVK LERPVIHIDQ TGENVLVETL 250
    NHELYEAKYV ISAVPPVLGM KIHFNPPLPM MRNQLITRVP LGSVIKSIVY 300
    YKEPFWRNMD YCGSMIIEGE EAPVAYALDD TKPDGSYPAI IGFILAHKAR 350
    KLARLTKEER LKKLCDLYAK VLGSQEALHP VHYEEKNWCE EQYSGGCYTS 400
    YFPPGIMTQY GRVLRQPVGR IYFAGTETAT HWSGYMEGAV EAGERAAREI 450
    LHAMGKIPED EIWLPEPESV DVPAKPITTT FLQRHLPSVP GLLKLIGLTT 500
    IFSATALGFL AHKRGLLVRI 520
    Length:520
    Mass (Da):58,421
    Last modified:January 23, 2007 - v4
    Checksum:iA594889B4495C410
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31S → N in AAF23179. 1 PublicationCurated
    Sequence conflicti270 – 2701M → G AA sequence (PubMed:2719656)Curated
    Sequence conflicti286 – 2861I → T in AAF23179. 1 PublicationCurated
    Sequence conflicti297 – 2993SIV → PIM AA sequence (PubMed:2719656)Curated
    Sequence conflicti307 – 3071R → K AA sequence (PubMed:2719656)Curated
    Sequence conflicti341 – 3411I → L AA sequence (PubMed:2719656)Curated
    Sequence conflicti344 – 3441I → K AA sequence (PubMed:2719656)Curated
    Sequence conflicti400 – 4001S → A AA sequence (PubMed:2719656)Curated
    Sequence conflicti478 – 4858TTTFLQRH → STSSMMMP AA sequence (PubMed:2719656)Curated
    Sequence conflicti509 – 5091F → Y in AAI19942. 1 PublicationCurated
    Sequence conflicti520 – 5201I → V in AAF23179. 1 PublicationCurated

    Mass spectrometryi

    Molecular mass is 59163.5±10.0 Da from positions 2 - 520. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF217955 mRNA. Translation: AAF23179.1.
    BC119941 mRNA. Translation: AAI19942.1.
    PIRiS07573.
    RefSeqiNP_808813.2. NM_177944.2.
    UniGeneiBt.22460.

    Genome annotation databases

    EnsembliENSBTAT00000001698; ENSBTAP00000001698; ENSBTAG00000001288.
    GeneIDi338445.
    KEGGibta:338445.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF217955 mRNA. Translation: AAF23179.1 .
    BC119941 mRNA. Translation: AAI19942.1 .
    PIRi S07573.
    RefSeqi NP_808813.2. NM_177944.2.
    UniGenei Bt.22460.

    3D structure databases

    ProteinModelPortali P56560.
    SMRi P56560. Positions 3-501.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P56560.
    ChEMBLi CHEMBL2756.

    Proteomic databases

    PaxDbi P56560.
    PRIDEi P56560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000001698 ; ENSBTAP00000001698 ; ENSBTAG00000001288 .
    GeneIDi 338445.
    KEGGi bta:338445.

    Organism-specific databases

    CTDi 4129.

    Phylogenomic databases

    eggNOGi COG1231.
    GeneTreei ENSGT00730000110903.
    HOGENOMi HOG000221615.
    HOVERGENi HBG004255.
    InParanoidi P56560.
    KOi K00274.
    OrthoDBi EOG7K6PTP.
    TreeFami TF313314.

    Enzyme and pathway databases

    BRENDAi 1.4.3.4. 908.
    Reactomei REACT_223515. Monoamines are oxidized to aldehydes by MAOA and MAOB, producing NH3 and H2O2.

    Miscellaneous databases

    NextBioi 20812644.

    Family and domain databases

    InterProi IPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00757. AMINEOXDASEF.
    ProtoNeti Search...

    Publicationsi

    1. Chung P.P., Vaidyanathan G., Lanier S.M.
      Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Basal ganglia.
    3. "High-level expression of human liver monoamine oxidase B in Pichia pastoris."
      Newton-Vinson P., Hubalek F., Edmondson D.E.
      Protein Expr. Purif. 20:334-345(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-17, ACETYLATION AT SER-2, MASS SPECTROMETRY.
    4. "The primary structure of bovine monoamine oxidase type A. Comparison with peptide sequences of bovine monoamine oxidase type B and other flavoenzymes."
      Powell J.F., Hsu Y.-P.P., Weyler W., Chen S.A., Salach J., Andrikopoulos K., Mallet J., Breakefield X.O.
      Biochem. J. 259:407-413(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-36; 53-70; 101-120; 259-279; 289-323; 327-346; 362-403; 420-431 AND 456-485.
    5. "Monoamine oxidase A from human placenta and monoamine oxidase B from bovine liver both have one FAD per subunit."
      Weyler W.
      Biochem. J. 260:725-729(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: DETERMINATION OF PROTEIN-FAD RATIO.
      Tissue: Liver.

    Entry informationi

    Entry nameiAOFB_BOVIN
    AccessioniPrimary (citable) accession number: P56560
    Secondary accession number(s): Q0P5K2, Q864W3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3