P56559 (ARL4C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-ribosylation factor-like protein 4C

Alternative name(s):
ADP-ribosylation factor-like protein 7
ADP-ribosylation factor-like protein LAK

Gene names

Name:	ARL4C
Synonyms:	ARL7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process. Ref.2 Ref.10 Ref.11
Subunit structure	Interacts with CYTH2. Interacts with alpha tubulin; interaction is independent on the ARL4C GTP or GDP binding status. Ref.10 Ref.11
Subcellular location	Cell projection › filopodium. Cell membrane. Cytoplasm Ref.2 Ref.10.
Tissue specificity	Expressed in several tumor cell lines (at protein level). Expressed in lung, brain, leukocytes and placenta. Ref.11
Induction	By liver X-receptor/retinoid X receptor agonists or cholesterol loading. Ref.2
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.
Sequence caution	The sequence BAA75473.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Cell projection Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endocytic recycling Inferred from direct assay Ref.11. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay Ref.11. Source: UniProtKB filopodium Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Traceable author statement. Source: ProtInc plasma membrane Inferred from direct assay Ref.10. Source: UniProtKB
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: ProtInc alpha-tubulin binding Inferred from direct assay Ref.11. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 192	191	ADP-ribosylation factor-like protein 4C		PRO_0000207462
Regions
	Nucleotide binding	20 – 27	8	GTP By similarity
	Nucleotide binding	68 – 72	5	GTP By similarity
	Nucleotide binding	127 – 130	4	GTP By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential
Experimental info
	Mutagenesis	2	1	G → A: Cytoplasmic localization. Ref.2 Ref.10
	Mutagenesis	27	1	T → N: Does not interact with alpha tubulin. Ref.11
	Mutagenesis	34	1	K → N: Cytoplasmic localization. Increases GDP-form. Ref.10
	Mutagenesis	72	1	Q → L: Does not interact with alpha tubulin. Activates transferrin transport from early endosome to recycling endosome. Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P56559 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 389BD49CF9E6D10D	FASTA	192	21,487
	10 20 30 40 50 60 MGNISSNISA FQSLHIVMLG LDSAGKTTVL YRLKFNEFVN TVPTIGFNTE KIKLSNGTAK 70 80 90 100 110 120 GISCHFWDVG GQEKLRPLWK SYSRCTDGII YVVDSVDVDR LEEAKTELHK VTKFAENQGT 130 140 150 160 170 180 PLLVIANKQD LPKSLPVAEI EKQLALHELI PATTYHVQPA CAIIGEGLTE GMDKLYEMIL 190 KRRKSLKQKK KR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ADP-ribosylation factor (ARF)-like 4, 6, and 7 represent a subgroup of the ARF family characterization by rapid nucleotide exchange and a nuclear localization signal." Jacobs S., Schilf C., Fliegert F., Koling S., Weber Y., Schurmann A., Joost H.-G. FEBS Lett. 456:384-388(1999) [PubMed: 10462049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"ADP-ribosylation factor (ARF)-like 7 (ARL7) is induced by cholesterol loading and participates in apolipoprotein AI-dependent cholesterol export." Engel T., Lueken A., Bode G., Hobohm U., Lorkowski S., Schlueter B., Rust S., Cullen P., Pech M., Assmann G., Seedorf U. FEBS Lett. 566:241-246(2004) [PubMed: 15147902] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, MUTAGENESIS OF GLY-2, SUBCELLULAR LOCATION.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Ovary.
[9]	"ADP-ribosylation factor-like protein LAK." Abe Y., Nezu K., Ueda N. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-192.
[10]	"The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane." Hofmann I., Thompson A., Sanderson C.M., Munro S. Curr. Biol. 17:711-716(2007) [PubMed: 17398095] [Abstract] Cited for: FUNCTION, INTERACTION WITH CYTH2, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-2 AND LYS-34.
[11]	"ADP-ribosylation factor like 7 (ARL7) interacts with alpha-tubulin and modulates intracellular vesicular transport." Wei S.M., Xie C.G., Abe Y., Cai J.T. Biochem. Biophys. Res. Commun. 384:352-356(2009) [PubMed: 19409876] [Abstract] Cited for: FUNCTION, INTERACTION WITH ALPHA TUBULIN, MUTAGENESIS OF THR-27 AND GLN-72, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y17804 mRNA. Translation: CAB44355.1. AJ579851 mRNA. Translation: CAE30323.1. AF493892 mRNA. Translation: AAM12606.1. BT019378 mRNA. Translation: AAV38185.1. BT019379 mRNA. Translation: AAV38186.1. AK314119 mRNA. Translation: BAG36810.1. AC097713 Genomic DNA. Translation: AAX93114.1. CH471063 Genomic DNA. Translation: EAW71073.1. BC001051 mRNA. No translation available. BC089043 mRNA. Translation: AAH89043.1. AB016811 mRNA. Translation: BAA75473.1. Different initiation.
IPI	IPI00936024.
RefSeq	NP_005728.2. NM_005737.3.
UniGene	Hs.111554. Hs.727031.
3D structure databases
ProteinModelPortal	P56559.
SMR	P56559. Positions 2-179.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-2867949.
STRING	P56559.
Polymorphism databases
DMDM	3913085.
Proteomic databases
PRIDE	P56559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000339728; ENSP00000339754; ENSG00000188042. ENST00000390645; ENSP00000375057; ENSG00000188042.
GeneID	10123.
KEGG	hsa:10123.
UCSC	uc002vvn.1. human.
Organism-specific databases
CTD	10123.
GeneCards	GC02M235401.
H-InvDB	HIX0002941.
HGNC	HGNC:698. ARL4C.
HPA	HPA028927.
MIM	604787. gene.
neXtProt	NX_P56559.
PharmGKB	PA24991.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11017.
GeneTree	ENSGT00600000084228.
HOVERGEN	HBG002073.
InParanoid	P56559.
OrthoDB	EOG483D5M.
PhylomeDB	P56559.
Gene expression databases
ArrayExpress	P56559.
Bgee	P56559.
CleanEx	HS_ARL4C.
Genevestigator	P56559.
GermOnline	ENSG00000188042. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR024156. Small_GTPase_ARF. IPR006689. Small_GTPase_ARF/SAR. [Graphical view]
KO	K07947.
PANTHER	PTHR11711. ARF/SAR. 1 hit.
Pfam	PF00025. Arf. 1 hit. [Graphical view]
PRINTS	PR00328. SAR1GTPBP.
SMART	SM00177. ARF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. Small_GTP. 1 hit.
PROSITE	PS51417. ARF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38287.
SOURCE	Search...

Entry information

Entry name

ARL4C_HUMAN

Accession

Primary (citable) accession number: P56559
Secondary accession number(s): Q53R10, Q9BVN1, Q9UQ34

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.