P56558 (OGT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
EC=2.4.1.-

Alternative name(s):
O-GlcNAc transferase subunit p110
O-linked N-acetylglucosamine transferase 110 kDa subunit

Gene names

Name:

Ogt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1036 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1 By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.
Enzyme regulation	By tyrosine phosphorylation and O-GlcNAc modifications.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Interacts directly with HCFC1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT By similarity. Heterotrimer of two 110 kDa and one 78 kDa subunits. It is not known if the 78 kDa subunit is encoded by a separate gene or is the product of either of a proteolytic degradation or an alternative initiation of the 110 kDa subunit. Interacts with ATXN10. Ref.2
Subcellular location	Cytoplasm. Nucleus By similarity. Note: Mostly in the nucleus By similarity. Ref.2
Tissue specificity	Appears to be present in all tissues examined except kidney.
Domain	The TPR repeat domain mediates recognition of protein substrates By similarity.
Post-translational modification	O-glycosylated; contains O-GlcNAc. Ubiquitinated, leading to its proteasomal degradation By similarity.
Sequence similarities	Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat TPR repeat
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation Glycoprotein Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of proteolysis Inferred from sequence or structural similarity. Source: UniProtKB protein O-linked glycosylation Inferred from direct assay Ref.1. Source: RGD protein homotrimerization Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay Ref.1. Source: RGD
Molecular function	monosaccharide binding Inferred from direct assay. Source: RGD peptide binding Inferred from direct assay. Source: RGD protein N-acetylglucosaminyltransferase activity Inferred from direct assay Ref.1. Source: RGD protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1036	1035	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit		PRO_0000191774
Regions
	Repeat	11 – 44	34	TPR 1
	Repeat	79 – 112	34	TPR 2
	Repeat	113 – 146	34	TPR 3
	Repeat	147 – 180	34	TPR 4
	Repeat	181 – 214	34	TPR 5
	Repeat	215 – 248	34	TPR 6
	Repeat	249 – 282	34	TPR 7
	Repeat	283 – 316	34	TPR 8
	Repeat	317 – 350	34	TPR 9
	Repeat	351 – 384	34	TPR 10
	Repeat	385 – 418	34	TPR 11
	Repeat	419 – 452	34	TPR 12
	Repeat	453 – 463	11	TPR 13; truncated
	Nucleotide binding	895 – 898	4	UDP By similarity
	Nucleotide binding	901 – 904	4	UDP By similarity
	Nucleotide binding	919 – 921	3	UDP By similarity
	Motif	478 – 493	16	Nuclear localization signal Potential
Sites
	Active site	498	1	Proton acceptor By similarity
	Binding site	839	1	UDP By similarity
	Binding site	842	1	UDP By similarity
	Binding site	925	1	UDP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	979	1	Phosphotyrosine Probable

Sequences

	Sequence		Length	Mass (Da)
	P56558 [UniParc]. Last modified December 15, 1998. Version 1. Checksum: 3F057CABDD019BD6	FASTA	1,036	115,606
	10 20 30 40 50 60 MASSVGNVAD STGLAELAHR EYQAGDFEAA ERHCMQLWRQ EPDNTGVLLL LSSIHFQCRR 70 80 90 100 110 120 LDRSAHFSTL AIKQNPLLAE AYSNLGNVYK ERGQLQEAIE HYRHALRLKP DFIDGYINLA 130 140 150 160 170 180 AALVAAGDME GAVQAYVSAL QYNPDLYCVR SDLGNLLKAL GRLEEAKACY LKAIETQPNF 190 200 210 220 230 240 AVAWSNLGCV FNAQGEIWLA IHHFEKAVTL DPNFLDAYIN LGNVLKEARI FDRAVAAYLR 250 260 270 280 290 300 ALSLSPNHAV VHGNLACVYY EQGLIDLAID TYRRAIELQP HFPDAYCNLA NALKEKGSVA 310 320 330 340 350 360 EAEDCYNTAL RLCPTHADSL NNLANIKREQ GNIEEAVRLY RKALEVFPEF AAAHSNLASV 370 380 390 400 410 420 LQQQGKLQEA LMHYKEAIRI SPTFADAYSN MGNTLKEMQD VQGALQCYTR AIQINPAFAD 430 440 450 460 470 480 AHSNLASIHK DSGNIPEAIA SYRTALKLKP DFPDAYCNLA HCLQIVCDWT DYDERMKKLV 490 500 510 520 530 540 SIVAEQLEKN RLPSVHPHHS MLYPLSHGFR KAIAERHGNL CLDKINVLHK PPYEHPKDLK 550 560 570 580 590 600 LSDGRLRVGY VSSDFGNHPT SHLMQSIPGM HNPDKFEVFC YALSPDDGTN FRVKVMAEAN 610 620 630 640 650 660 HFIDLSQIPC NGKAADRIHQ DGIHILVNMN GYTKGARNEL FALRPAPIQA MWLGYPGTSG 670 680 690 700 710 720 ALFMDYIITD QETSPAEVAE QYSEKLAYMP HTFFIGDHAN MFPHLKKKAV IDFKSNGHIY 730 740 750 760 770 780 DNRIVLNGID LKAFLDSLPD VKIVKMKCPD GGDNADTTNT ALNMPVIPMN TIAEAVIEMI 790 800 810 820 830 840 NRGQIQITIN GFSISNGLAT TQINNKAATG EEVPRTIIVT TRSQYGLPED AIVYCNFNQL 850 860 870 880 890 900 YKIDPSTLQM GANILKRVPN SVLWLLRFPA VGEPNIQQYA QNMGLPQNRI IFSPVAPKEE 910 920 930 940 950 960 HVRRGQLADV CLDTPLCNGH TTGMDVLWAG TPMVTMPGET LASRVAASQL TCLGCLELIA 970 980 990 1000 1010 1020 KSRQEYEDIA VKLGTDLEYL KKIRGKVWKQ RISSPLFNTK QYTMELERLY LQMWEHYAAG 1030 NKPDHMIKPV EVTESA

References

[1]	"Dynamic glycosylation of nuclear and cytosolic proteins. Cloning and characterization of a unique O-GlcNAc transferase with multiple tetratricopeptide repeats." Kreppel L.K., Blomberg M.A., Hart G.W. J. Biol. Chem. 272:9308-9315(1997) [PubMed: 9083067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Ataxin-10 interacts with O-linked beta-N-acetylglucosamine transferase in the brain." Maerz P., Stetefeld J., Bendfeldt K., Nitsch C., Reinstein J., Shoeman R.L., Dimitriades-Schmutz B., Schwager M., Leiser D., Ozcan S., Otten U., Ozbek S. J. Biol. Chem. 281:20263-20270(2006) [PubMed: 16714295] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ATXN10.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U76557 mRNA. Translation: AAC53121.1.
IPI	IPI00231503.
PIR	T31673.
RefSeq	NP_058803.2. NM_017107.2.
UniGene	Rn.82705.
3D structure databases
ProteinModelPortal	P56558.
SMR	P56558. Positions 13-400.
ModBase	Search...
Protein-protein interaction databases
STRING	P56558.
Protein family/group databases
CAZy	GT41. Glycosyltransferase Family 41.
PTM databases
PhosphoSite	P56558.
Proteomic databases
PRIDE	P56558.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	26295.
KEGG	rno:26295.
UCSC	NM_017107. rat.
Organism-specific databases
CTD	8473.
RGD	62060. Ogt.
Phylogenomic databases
eggNOG	roNOG07233.
GeneTree	ENSGT00550000074327.
HOVERGEN	HBG000351.
InParanoid	P56558.
OrthoDB	EOG4HQDHJ.
PhylomeDB	P56558.
Enzyme and pathway databases
BRENDA	2.4.1.94. 5301.
Gene expression databases
ArrayExpress	P56558.
Genevestigator	P56558.
GermOnline	ENSRNOG00000003359. Rattus norvegicus.
Family and domain databases
InterPro	IPR001440. TPR-1. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 2 hits.
KO	K09667.
Pfam	PF00515. TPR_1. 10 hits. [Graphical view]
SMART	SM00028. TPR. 11 hits. [Graphical view]
PROSITE	PS50005. TPR. 12 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608004.

Entry information

Entry name

OGT1_RAT

Accession

Primary (citable) accession number: P56558

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	November 16, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program