ID   DEF_PENBA               Reviewed;          54 AA.
AC   P56552;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   13-SEP-2023, entry version 89.
DE   RecName: Full=Defensin-like protein;
DE   AltName: Full=Brazzein {ECO:0000303|PubMed:7957951};
OS   Pentadiplandra brazzeana.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Pentadiplandraceae; Pentadiplandra.
OX   NCBI_TaxID=43545;
RN   [1]
RP   PROTEIN SEQUENCE, AND PYROGLUTAMATE FORMATION AT GLN-1.
RC   TISSUE=Fruit;
RX   PubMed=7957951; DOI=10.1016/0014-5793(94)01184-2;
RA   Ming D., Hellekant G.;
RT   "Brazzein, a new high-potency thermostable sweet protein from
RT   Pentadiplandra brazzeana B.";
RL   FEBS Lett. 355:106-108(1994).
RN   [2]
RP   FUNCTION.
RX   PubMed=15118082; DOI=10.1073/pnas.0401567101;
RA   Yount N.Y., Yeaman M.R.;
RT   "Multidimensional signatures in antimicrobial peptides.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7363-7368(2004).
RN   [3]
RP   STRUCTURE BY NMR.
RX   PubMed=9628478; DOI=10.1038/nsb0698-427;
RA   Caldwell J.E., Abildgaard F., Dzakula Z., Ming D., Hellekant G.,
RA   Markley J.L.;
RT   "Solution structure of the thermostable sweet-tasting protein brazzein.";
RL   Nat. Struct. Biol. 5:427-431(1998).
CC   -!- FUNCTION: Taste-modifying protein; sweet-tasting. It is 2000 sweeter
CC       than sucrose on a molar basis. {ECO:0000269|PubMed:15118082}.
CC   -!- FUNCTION: Has a pH-specific antimicrobial activity against bacteria
CC       (B.subtilis, E.coli and S.aureus) and the fungus C.albicans.
CC       {ECO:0000269|PubMed:15118082}.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR   PIR; S51208; S51208.
DR   PDB; 1BRZ; NMR; -; A=2-54.
DR   PDB; 2BRZ; NMR; -; A=2-54.
DR   PDB; 2KGQ; NMR; -; A=2-54.
DR   PDB; 2KYQ; NMR; -; A=3-54.
DR   PDB; 2LY5; NMR; -; A=2-54.
DR   PDB; 2LY6; NMR; -; A=2-54.
DR   PDB; 2N66; NMR; -; A=1-54.
DR   PDB; 2N69; NMR; -; A=1-54.
DR   PDB; 4HE7; X-ray; 1.80 A; A=1-54.
DR   PDB; 7W8E; X-ray; 1.34 A; A=1-54.
DR   PDB; 7W8H; X-ray; 1.50 A; A/B/C/D/E/F/G/H=1-54.
DR   PDBsum; 1BRZ; -.
DR   PDBsum; 2BRZ; -.
DR   PDBsum; 2KGQ; -.
DR   PDBsum; 2KYQ; -.
DR   PDBsum; 2LY5; -.
DR   PDBsum; 2LY6; -.
DR   PDBsum; 2N66; -.
DR   PDBsum; 2N69; -.
DR   PDBsum; 4HE7; -.
DR   PDBsum; 7W8E; -.
DR   PDBsum; 7W8H; -.
DR   AlphaFoldDB; P56552; -.
DR   BMRB; P56552; -.
DR   SMR; P56552; -.
DR   TCDB; 1.C.45.4.2; the plant defensin (plant defensin) family.
DR   EvolutionaryTrace; P56552; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; Knottin, scorpion toxin-like; 1.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SUPFAM; SSF57095; Scorpion toxin-like; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Pyrrolidone carboxylic acid; Secreted;
KW   Taste-modifying protein.
FT   CHAIN           1..54
FT                   /note="Defensin-like protein"
FT                   /id="PRO_0000221427"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:7957951"
FT   DISULFID        4..52
FT   DISULFID        16..37
FT   DISULFID        22..47
FT   DISULFID        26..49
FT   STRAND          3..7
FT                   /evidence="ECO:0007829|PDB:7W8H"
FT   HELIX           13..17
FT                   /evidence="ECO:0007829|PDB:7W8H"
FT   HELIX           21..30
FT                   /evidence="ECO:0007829|PDB:7W8H"
FT   STRAND          33..39
FT                   /evidence="ECO:0007829|PDB:7W8H"
FT   STRAND          41..43
FT                   /evidence="ECO:0007829|PDB:2BRZ"
FT   STRAND          45..52
FT                   /evidence="ECO:0007829|PDB:7W8H"
SQ   SEQUENCE   54 AA;  6498 MW;  5BAB4D7215291252 CRC64;
     QDKCKKVYEN YPVSKCQLAN QCNYDCKLDK HARSGECFYD EKRNLQCICD YCEY
//