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P56552

- DEF_PENBA

UniProt

P56552 - DEF_PENBA

Protein

Defensin-like protein

Gene
N/A
Organism
Pentadiplandra brazzeana
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Taste-modifying protein; sweet-tasting. It is 2000 sweeter than sucrose on a molar basis.1 Publication
    Has a pH-specific antimicrobial activity against bacteria (B.subtilis, E.coli and S.aureus) and the fungus C.albicans.1 Publication

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. defense response to fungus Source: UniProtKB-KW
    3. killing of cells of other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Fungicide, Taste-modifying protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Defensin-like protein
    Alternative name(s):
    Brazzein
    OrganismiPentadiplandra brazzeana
    Taxonomic identifieri43545 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesPentadiplandraceaePentadiplandra

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5454Defensin-like proteinPRO_0000221427Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi4 ↔ 52
    Disulfide bondi16 ↔ 37
    Disulfide bondi22 ↔ 47
    Disulfide bondi26 ↔ 49

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    54
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Helixi13 – 164
    Beta strandi17 – 204
    Helixi21 – 3010
    Beta strandi33 – 397
    Beta strandi41 – 433
    Beta strandi45 – 528

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BRZNMR-A2-54[»]
    2BRZNMR-A2-54[»]
    2KGQNMR-A2-54[»]
    2KYQNMR-A3-54[»]
    2LY5NMR-A2-54[»]
    2LY6NMR-A2-54[»]
    4HE7X-ray1.80A1-54[»]
    DisProtiDP00582.
    ProteinModelPortaliP56552.
    SMRiP56552. Positions 1-54.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56552.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DEFL family.Curated

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    [Graphical view]
    SUPFAMiSSF57095. SSF57095. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P56552-1 [UniParc]FASTAAdd to Basket

    « Hide

    QDKCKKVYEN YPVSKCQLAN QCNYDCKLDK HARSGECFYD EKRNLQCICD   50
    YCEY 54
    Length:54
    Mass (Da):6,498
    Last modified:July 15, 1998 - v1
    Checksum:i5BAB4D7215291252
    GO

    Sequence databases

    PIRiS51208.

    Cross-referencesi

    Sequence databases

    PIRi S51208.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BRZ NMR - A 2-54 [» ]
    2BRZ NMR - A 2-54 [» ]
    2KGQ NMR - A 2-54 [» ]
    2KYQ NMR - A 3-54 [» ]
    2LY5 NMR - A 2-54 [» ]
    2LY6 NMR - A 2-54 [» ]
    4HE7 X-ray 1.80 A 1-54 [» ]
    DisProti DP00582.
    ProteinModelPortali P56552.
    SMRi P56552. Positions 1-54.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P56552.

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    [Graphical view ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B."
      Ming D., Hellekant G.
      FEBS Lett. 355:106-108(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Tissue: Fruit.
    2. "Multidimensional signatures in antimicrobial peptides."
      Yount N.Y., Yeaman M.R.
      Proc. Natl. Acad. Sci. U.S.A. 101:7363-7368(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "Solution structure of the thermostable sweet-tasting protein brazzein."
      Caldwell J.E., Abildgaard F., Dzakula Z., Ming D., Hellekant G., Markley J.L.
      Nat. Struct. Biol. 5:427-431(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiDEF_PENBA
    AccessioniPrimary (citable) accession number: P56552
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3