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P56552 (DEF_PENBA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Defensin-like protein
Alternative name(s):
Brazzein
OrganismPentadiplandra brazzeana
Taxonomic identifier43545 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesPentadiplandraceaePentadiplandra

Protein attributes

Sequence length54 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Taste-modifying protein; sweet-tasting. It is 2000 sweeter than sucrose on a molar basis. Ref.2

Has a pH-specific antimicrobial activity against bacteria (B.subtilis, E.coli and S.aureus) and the fungus C.albicans. Ref.2

Subunit structure

Monomer.

Subcellular location

Secreted.

Sequence similarities

Belongs to the DEFL family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5454Defensin-like protein
PRO_0000221427

Amino acid modifications

Modified residue11Pyrrolidone carboxylic acid Ref.1
Disulfide bond4 ↔ 52
Disulfide bond16 ↔ 37
Disulfide bond22 ↔ 47
Disulfide bond26 ↔ 49

Secondary structure

............. 54
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56552 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 5BAB4D7215291252

FASTA546,498
        10         20         30         40         50 
QDKCKKVYEN YPVSKCQLAN QCNYDCKLDK HARSGECFYD EKRNLQCICD YCEY 

« Hide

References

[1]"Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B."
Ming D., Hellekant G.
FEBS Lett. 355:106-108(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Tissue: Fruit.
[2]"Multidimensional signatures in antimicrobial peptides."
Yount N.Y., Yeaman M.R.
Proc. Natl. Acad. Sci. U.S.A. 101:7363-7368(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[3]"Solution structure of the thermostable sweet-tasting protein brazzein."
Caldwell J.E., Abildgaard F., Dzakula Z., Ming D., Hellekant G., Markley J.L.
Nat. Struct. Biol. 5:427-431(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRS51208.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRZNMR-A2-54[»]
2BRZNMR-A2-54[»]
2KGQNMR-A2-54[»]
2KYQNMR-A6-54[»]
2LY5NMR-A2-54[»]
2LY6NMR-A2-54[»]
4HE7X-ray1.80A2-54[»]
DisProtDP00582.
ProteinModelPortalP56552.
SMRP56552. Positions 1-54.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
[Graphical view]
SUPFAMSSF57095. SSF57095. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP56552.

Entry information

Entry nameDEF_PENBA
AccessionPrimary (citable) accession number: P56552
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references