P56552 (DEF_PENBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Defensin-like protein Alternative name(s): Brazzein
Organism	Pentadiplandra brazzeana
Taxonomic identifier	43545 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Pentadiplandraceae › Pentadiplandra

Protein attributes

Sequence length	54 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Taste-modifying protein; sweet-tasting. It is 2000 sweeter than sucrose on a molar basis. Ref.2 Has a pH-specific antimicrobial activity against bacteria (B.subtilis, E.coli and S.aureus) and the fungus C.albicans. Ref.2
Subunit structure	Monomer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the DEFL family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Antibiotic Antimicrobial Fungicide Taste-modifying protein
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 54

54

Defensin-like protein

PRO_0000221427

Amino acid modifications

Modified residue

1

1

Pyrrolidone carboxylic acid Ref.1

Disulfide bond

4 ↔ 52

Disulfide bond

16 ↔ 37

Disulfide bond

22 ↔ 47

Disulfide bond

26 ↔ 49

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

54

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Helix

13 – 16

4

Beta strand

17 – 20

4

Helix

21 – 29

9

Beta strand

33 – 38

6

Beta strand

41 – 43

3

Beta strand

46 – 52

7

Sequences

	Sequence		Length	Mass (Da)
	P56552 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 5BAB4D7215291252	FASTA	54	6,498
	10 20 30 40 50 QDKCKKVYEN YPVSKCQLAN QCNYDCKLDK HARSGECFYD EKRNLQCICD YCEY

References

[1]	"Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B." Ming D., Hellekant G. FEBS Lett. 355:106-108(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Fruit.
[2]	"Multidimensional signatures in antimicrobial peptides." Yount N.Y., Yeaman M.R. Proc. Natl. Acad. Sci. U.S.A. 101:7363-7368(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Solution structure of the thermostable sweet-tasting protein brazzein." Caldwell J.E., Abildgaard F., Dzakula Z., Ming D., Hellekant G., Markley J.L. Nat. Struct. Biol. 5:427-431(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S51208.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BRZ	NMR	-	A	2-54	[»]
2BRZ	NMR	-	A	2-54	[»]
2KGQ	NMR	-	A	2-54	[»]
2KYQ	NMR	-	A	6-54	[»]
2LY5	NMR	-	A	2-54	[»]
2LY6	NMR	-	A	2-54	[»]

ProteinModelPortal

P56552.

SMR

P56552. Positions 1-54.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.30.10. 1 hit.

InterPro

IPR003614. Scorpion_toxin-like.
[Graphical view]

SUPFAM

SSF57095. SSF57095. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P56552.

Entry information

Entry name

DEF_PENBA

Accession

Primary (citable) accession number: P56552

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program