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Protein

Defensin-like protein

Gene
N/A
Organism
Pentadiplandra brazzeana
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Taste-modifying protein; sweet-tasting. It is 2000 sweeter than sucrose on a molar basis.1 Publication
Has a pH-specific antimicrobial activity against bacteria (B.subtilis, E.coli and S.aureus) and the fungus C.albicans.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide, Taste-modifying protein

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-like protein
Alternative name(s):
Brazzein
OrganismiPentadiplandra brazzeana
Taxonomic identifieri43545 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesPentadiplandraceaePentadiplandra

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5454Defensin-like proteinPRO_0000221427Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11Pyrrolidone carboxylic acid1 Publication
Disulfide bondi4 ↔ 52
Disulfide bondi16 ↔ 37
Disulfide bondi22 ↔ 47
Disulfide bondi26 ↔ 49

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
54
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Helixi13 – 164Combined sources
Beta strandi17 – 204Combined sources
Helixi21 – 3010Combined sources
Beta strandi33 – 397Combined sources
Beta strandi41 – 433Combined sources
Beta strandi45 – 528Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRZNMR-A2-54[»]
2BRZNMR-A2-54[»]
2KGQNMR-A2-54[»]
2KYQNMR-A3-54[»]
2LY5NMR-A2-54[»]
2LY6NMR-A2-54[»]
4HE7X-ray1.80A1-54[»]
DisProtiDP00582.
ProteinModelPortaliP56552.
SMRiP56552. Positions 1-54.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56552.

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

P56552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QDKCKKVYEN YPVSKCQLAN QCNYDCKLDK HARSGECFYD EKRNLQCICD

YCEY
Length:54
Mass (Da):6,498
Last modified:July 15, 1998 - v1
Checksum:i5BAB4D7215291252
GO

Sequence databases

PIRiS51208.

Cross-referencesi

Sequence databases

PIRiS51208.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BRZNMR-A2-54[»]
2BRZNMR-A2-54[»]
2KGQNMR-A2-54[»]
2KYQNMR-A3-54[»]
2LY5NMR-A2-54[»]
2LY6NMR-A2-54[»]
4HE7X-ray1.80A1-54[»]
DisProtiDP00582.
ProteinModelPortaliP56552.
SMRiP56552. Positions 1-54.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP56552.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B."
    Ming D., Hellekant G.
    FEBS Lett. 355:106-108(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Fruit.
  2. "Multidimensional signatures in antimicrobial peptides."
    Yount N.Y., Yeaman M.R.
    Proc. Natl. Acad. Sci. U.S.A. 101:7363-7368(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Solution structure of the thermostable sweet-tasting protein brazzein."
    Caldwell J.E., Abildgaard F., Dzakula Z., Ming D., Hellekant G., Markley J.L.
    Nat. Struct. Biol. 5:427-431(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiDEF_PENBA
AccessioniPrimary (citable) accession number: P56552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: January 7, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.