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Reviewed, UniProtKB/Swiss-Prot P56546 (CTBP2_MOUSE)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    C-terminal-binding protein 2
      Short name=CtBP2
Gene names
Name: Ctbp2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses By similarity.

Subunit structure

Interacts with HIPK2 and PNN By similarity. Interacts with the transcription factors BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Interacts also with the C-terminus of adenovirus E1A protein. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ.

Subcellular location

Nucleus Potential. Cell junction › synapse By similarity.

Tissue specificity

Found in all tissues except spleen and liver.

Developmental stage

Strong expression confined to the embryonic stages.

Post-translational modification

Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-171 and Ser-177. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation By similarity.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Sequence caution

The sequence AAC40043.1 differs from that shown. Reason: Frameshift at position 12.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

EVI1Q031123EBI-1384883,EBI-1384862From a different organism.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P56546-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P56546-2)

Also known as: Ribeye;

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS
Note: Contains a phosphothreonine at position 171 (By similarity). Contains a phosphoserine at position 177 (By similarity).

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445C-terminal-binding protein 2
PRO_0000076045

Sites

Active site2721 Potential
Active site3011 Potential
Active site3211 Potential

Amino acid modifications

Modified residue4281Phosphoserine; by HIPK2 By similarity

Natural variations

Alternative sequence1 – 2020MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGTWCELLNHRAQDTES YLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGMLGSRMPW EQVQGQLPALQDTGHLYPES GGKTVPHGQRTHGRAPSPGR YGREQPDTRLGIEVPTYSPN SSQVYNDICERPVDSTPARQ VAPTCLVVDPSSAVPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTMYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLA SSCRTEMQFRRQDRTGPLPR NRSSSFSHRSELLPNDMASL GTTALQFHPAGPLQTPSPRA GDLGRRPSSAPSQHLLETAA TYSTPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSAPLHSNPRT AYSTSYTVPMELLKRERSMT ASPLPSPHGSPQLLRKPGAA PVEPSTLPPVSQSLHTPHSP YQKVARRTGAPIIVSTMLTP EPS in isoform 2.
VSP_027616

Experimental info

Sequence conflict491P → H in AAC40043. Ref.1
Sequence conflict1701E → D in AAC40043. Ref.1
Sequence conflict2881G → A in AAC40043. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 5A81B9D75A9E493B

FASTA44548,957
        10         20         30         40         50         60 
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI LKDLATVAFC 

        70         80         90        100        110        120 
DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV IVRIGSGYDN VDIKAAGELG 

       130        140        150        160        170        180 
IAVCNIPSAA VEETADSTVC HILNLYRRNT WLYQALREGT RVQSVEQIRE VASGAARIRG 

       190        200        210        220        230        240 
ETLGLIGFGR TGQAVAVRAK AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS 

       250        260        270        280        290        300 
LHCNLNEHNH HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES 

       310        320        330        340        350        360 
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP ESLRNCVNKE 

       370        380        390        400        410        420 
FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP PAMEGIIPGG IPVTHNLPTV 

       430        440 
AHPSQAPSPN QPTKHGDNRE HPNEQ

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Isoform 2 (Ribeye).

Checksum: EF7CF59169C5A649
Show »

FASTA988107,127

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References

« Hide 'large scale' references
[1]"A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3."
Katsanis N., Fisher E.M.C.
Genomics 47:294-299(1998) [PubMed: 9479502] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning and characterization of mCtBP2, a co-repressor that associates with basic krueppel-like factor and other mammalian transcriptional regulators."
Turner J., Crossley M.
EMBO J. 17:5129-5140(1998) [PubMed: 9724649] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1."
Furusawa T., Moribe H., Kondoh H., Higashi Y.
Mol. Cell. Biol. 19:8581-8590(1999) [PubMed: 10567582] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: ICR.
[4]The mouse genome sequencing consortium
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], LATERNATIVE SPLICING (ISOFORM 2).
Strain: C57BL/6J.
[5]"Characterization of four autonomous repression domains in the corepressor receptor interacting protein 140."
Christian M., Tullet J.M.A., Parker M.G.
J. Biol. Chem. 279:15645-15651(2004) [PubMed: 14736873] [Abstract]
Cited for: INTERACTION WITH NRIP1.
[6]"Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP."
Ueda J., Tachibana M., Ikura T., Shinkai Y.
J. Biol. Chem. 281:20120-20128(2006) [PubMed: 16702210] [Abstract]
Cited for: INTERACTION WITH WIZ.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF016508 mRNA. Translation: AAC40043.1. Frameshift.
AF059735 mRNA. Translation: AAC33873.1.
AB033123 mRNA. Translation: BAA85181.1.
AC119806 Genomic DNA. No translation available.
IPIIPI00114237.
IPI00856974.
RefSeqNP_034110.1.
UniGeneMm.246240
Mm.389984

3D structure databases

HSSPHSSP built from PDB template 1MX3 based on UniProtKB Q13363.
SMRP56546. Positions 33-362.
ModBaseSearch...

Protein-protein interaction databases

IntActP56546. 1 interaction.

PTM databases

PhosphoSiteP56546.

2-D gel databases

REPRODUCTION-2DPAGEP56546.

Proteomic databases

PRIDEP56546.

Genome annotation databases

EnsemblENSMUSG00000030970. Mus musculus. [Contig view]
GeneID13017.
KEGGmmu:13017.

Organism-specific databases

MGIMGI:1201686. Ctbp2.

Phylogenomic databases

HOGENOMP56546.
HOVERGENP56546.
OMAP56546. TMCLILN.

Gene expression databases

ArrayExpressP56546.
BgeeP56546.
CleanExMM_CTBP2.
GermOnlineENSMUSG00000030970. Mus musculus.

Family and domain databases

InterProIPR006139. D-isomer_2_OHA_DH.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. False negative.
PS00670. D_2_HYDROXYACID_DH_2. False negative.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio282874.
SOURCESearch...

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Entry information

Entry nameCTBP2_MOUSE
AccessionPrimary (citable) accession number: P56546
Secondary accession number(s): O54855, O88462
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents