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P56546

- CTBP2_MOUSE

UniProt

P56546 - CTBP2_MOUSE

Protein

C-terminal-binding protein 2

Gene

Ctbp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses By similarity. Functions in brown adipose tissue (BAT) differentiation.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei106 – 1061NADBy similarity
    Binding sitei210 – 2101NADBy similarity
    Active sitei272 – 2721By similarity
    Binding sitei296 – 2961NADBy similarity
    Active sitei301 – 3011By similarity
    Active sitei321 – 3211Proton donorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi186 – 1916NADBy similarity
    Nucleotide bindingi243 – 2497NADBy similarity
    Nucleotide bindingi270 – 2723NADBy similarity
    Nucleotide bindingi321 – 3244NADBy similarity

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
    3. protein binding Source: UniProtKB
    4. transcription corepressor activity Source: MGI

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: UniProtKB
    2. transcription, DNA-templated Source: UniProtKB-KW
    3. white fat cell differentiation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase, Repressor

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-terminal-binding protein 2
    Short name:
    CtBP2
    Gene namesi
    Name:Ctbp2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1201686. Ctbp2.

    Subcellular locationi

    Nucleus Curated. Cell junctionsynapse By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. nucleus Source: UniProtKB
    3. synapse Source: MGI
    4. transcriptional repressor complex Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Nucleus, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 445445C-terminal-binding protein 2PRO_0000076045Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei428 – 4281Phosphoserine; by HIPK2By similarity

    Post-translational modificationi

    Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP56546.
    PaxDbiP56546.
    PRIDEiP56546.

    2D gel databases

    REPRODUCTION-2DPAGEP56546.

    PTM databases

    PhosphoSiteiP56546.

    Expressioni

    Tissue specificityi

    Found in all tissues except spleen and liver.

    Developmental stagei

    Strong expression confined to the embryonic stages.

    Gene expression databases

    ArrayExpressiP56546.
    BgeeiP56546.
    CleanExiMM_CTBP2.
    GenevestigatoriP56546.

    Interactioni

    Subunit structurei

    Interacts with HIPK2 and PNN By similarity. Interacts with the transcription factors ZNF217, BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Interacts also with the C-terminus of adenovirus E1A protein. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HIC1Q145262EBI-1384883,EBI-2507362From a different organism.
    MECOMQ031123EBI-1384883,EBI-1384862From a different organism.
    PRDM16Q9HAZ2-22EBI-1384883,EBI-5282871From a different organism.
    PRDM16Q9HAZ2-42EBI-1384883,EBI-4566658From a different organism.

    Protein-protein interaction databases

    BioGridi198962. 19 interactions.
    IntActiP56546. 7 interactions.
    MINTiMINT-4084830.

    Structurei

    3D structure databases

    ProteinModelPortaliP56546.
    SMRiP56546. Positions 33-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0111.
    GeneTreeiENSGT00530000063021.
    HOGENOMiHOG000136701.
    HOVERGENiHBG001898.
    InParanoidiP56546.
    KOiK04496.
    OMAiEDADIKC.
    OrthoDBiEOG761BT9.
    PhylomeDBiP56546.
    TreeFamiTF313593.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
    IPR006140. D-isomer_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00389. 2-Hacid_dh. 1 hit.
    PF02826. 2-Hacid_dh_C. 1 hit.
    [Graphical view]
    PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P56546-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI    50
    LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV 100
    IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTVC HILNLYRRNT 150
    WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK 200
    AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH 250
    HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES 300
    EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP 350
    ESLRNCVNKE FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP 400
    PAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ 445
    Length:445
    Mass (Da):48,957
    Last modified:December 1, 2000 - v2
    Checksum:i5A81B9D75A9E493B
    GO
    Isoform 2 (identifier: P56546-2) [UniParc]FASTAAdd to Basket

    Also known as: Ribeye

    The sequence of this isoform differs from the canonical sequence as follows:
         1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS

    Show »
    Length:988
    Mass (Da):107,127
    Checksum:iEF7CF59169C5A649
    GO

    Sequence cautioni

    The sequence AAC40043.1 differs from that shown. Reason: Frameshift at position 12.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti49 – 491P → H in AAC40043. (PubMed:9479502)Curated
    Sequence conflicti170 – 1701E → D in AAC40043. (PubMed:9479502)Curated
    Sequence conflicti288 – 2881G → A in AAC40043. (PubMed:9479502)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2020MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGTWCELLNHRAQDTES YLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGMLGSRMPW EQVQGQLPALQDTGHLYPES GGKTVPHGQRTHGRAPSPGR YGREQPDTRLGIEVPTYSPN SSQVYNDICERPVDSTPARQ VAPTCLVVDPSSAVPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTMYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLA SSCRTEMQFRRQDRTGPLPR NRSSSFSHRSELLPNDMASL GTTALQFHPAGPLQTPSPRA GDLGRRPSSAPSQHLLETAA TYSTPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSAPLHSNPRT AYSTSYTVPMELLKRERSMT ASPLPSPHGSPQLLRKPGAA PVEPSTLPPVSQSLHTPHSP YQKVARRTGAPIIVSTMLTP EPS in isoform 2. CuratedVSP_027616Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF016508 mRNA. Translation: AAC40043.1. Frameshift.
    AF059735 mRNA. Translation: AAC33873.1.
    AB033123 mRNA. Translation: BAA85181.1.
    AC119806 Genomic DNA. No translation available.
    CCDSiCCDS21930.1. [P56546-1]
    CCDS52417.1. [P56546-2]
    RefSeqiNP_001164215.1. NM_001170744.1. [P56546-2]
    NP_034110.1. NM_009980.4. [P56546-1]
    XP_006507371.1. XM_006507308.1. [P56546-1]
    XP_006507372.1. XM_006507309.1. [P56546-1]
    XP_006507373.1. XM_006507310.1. [P56546-1]
    XP_006507374.1. XM_006507311.1. [P56546-1]
    XP_006507375.1. XM_006507312.1. [P56546-1]
    XP_006507376.1. XM_006507313.1. [P56546-1]
    UniGeneiMm.246240.
    Mm.389984.

    Genome annotation databases

    EnsembliENSMUST00000033269; ENSMUSP00000033269; ENSMUSG00000030970. [P56546-1]
    ENSMUST00000169570; ENSMUSP00000130294; ENSMUSG00000030970. [P56546-2]
    GeneIDi13017.
    KEGGimmu:13017.
    UCSCiuc009kcy.1. mouse. [P56546-1]
    uc012fve.1. mouse. [P56546-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF016508 mRNA. Translation: AAC40043.1 . Frameshift.
    AF059735 mRNA. Translation: AAC33873.1 .
    AB033123 mRNA. Translation: BAA85181.1 .
    AC119806 Genomic DNA. No translation available.
    CCDSi CCDS21930.1. [P56546-1 ]
    CCDS52417.1. [P56546-2 ]
    RefSeqi NP_001164215.1. NM_001170744.1. [P56546-2 ]
    NP_034110.1. NM_009980.4. [P56546-1 ]
    XP_006507371.1. XM_006507308.1. [P56546-1 ]
    XP_006507372.1. XM_006507309.1. [P56546-1 ]
    XP_006507373.1. XM_006507310.1. [P56546-1 ]
    XP_006507374.1. XM_006507311.1. [P56546-1 ]
    XP_006507375.1. XM_006507312.1. [P56546-1 ]
    XP_006507376.1. XM_006507313.1. [P56546-1 ]
    UniGenei Mm.246240.
    Mm.389984.

    3D structure databases

    ProteinModelPortali P56546.
    SMRi P56546. Positions 33-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198962. 19 interactions.
    IntActi P56546. 7 interactions.
    MINTi MINT-4084830.

    PTM databases

    PhosphoSitei P56546.

    2D gel databases

    REPRODUCTION-2DPAGE P56546.

    Proteomic databases

    MaxQBi P56546.
    PaxDbi P56546.
    PRIDEi P56546.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033269 ; ENSMUSP00000033269 ; ENSMUSG00000030970 . [P56546-1 ]
    ENSMUST00000169570 ; ENSMUSP00000130294 ; ENSMUSG00000030970 . [P56546-2 ]
    GeneIDi 13017.
    KEGGi mmu:13017.
    UCSCi uc009kcy.1. mouse. [P56546-1 ]
    uc012fve.1. mouse. [P56546-2 ]

    Organism-specific databases

    CTDi 1488.
    MGIi MGI:1201686. Ctbp2.

    Phylogenomic databases

    eggNOGi COG0111.
    GeneTreei ENSGT00530000063021.
    HOGENOMi HOG000136701.
    HOVERGENi HBG001898.
    InParanoidi P56546.
    KOi K04496.
    OMAi EDADIKC.
    OrthoDBi EOG761BT9.
    PhylomeDBi P56546.
    TreeFami TF313593.

    Miscellaneous databases

    ChiTaRSi CTBP2. mouse.
    NextBioi 282874.
    PROi P56546.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P56546.
    Bgeei P56546.
    CleanExi MM_CTBP2.
    Genevestigatori P56546.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR006139. D-isomer_2_OHA_DH_cat_dom.
    IPR006140. D-isomer_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00389. 2-Hacid_dh. 1 hit.
    PF02826. 2-Hacid_dh_C. 1 hit.
    [Graphical view ]
    PROSITEi PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3."
      Katsanis N., Fisher E.M.C.
      Genomics 47:294-299(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Cloning and characterization of mCtBP2, a co-repressor that associates with basic krueppel-like factor and other mammalian transcriptional regulators."
      Turner J., Crossley M.
      EMBO J. 17:5129-5140(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1."
      Furusawa T., Moribe H., Kondoh H., Higashi Y.
      Mol. Cell. Biol. 19:8581-8590(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: ICR.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
      Strain: C57BL/6J.
    5. "Characterization of four autonomous repression domains in the corepressor receptor interacting protein 140."
      Christian M., Tullet J.M.A., Parker M.G.
      J. Biol. Chem. 279:15645-15651(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NRIP1.
    6. "Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP."
      Ueda J., Tachibana M., Ikura T., Shinkai Y.
      J. Biol. Chem. 281:20120-20128(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH WIZ.
    7. "Specific recognition of ZNF217 and other zinc finger proteins at a surface groove of C-terminal binding proteins."
      Quinlan K.G.R., Nardini M., Verger A., Francescato P., Yaswen P., Corda D., Bolognesi M., Crossley M.
      Mol. Cell. Biol. 26:8159-8172(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZNF217.
    8. "Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex."
      Kajimura S., Seale P., Tomaru T., Erdjument-Bromage H., Cooper M.P., Ruas J.L., Chin S., Tempst P., Lazar M.A., Spiegelman B.M.
      Genes Dev. 22:1397-1409(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PRDM16, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCTBP2_MOUSE
    AccessioniPrimary (citable) accession number: P56546
    Secondary accession number(s): O54855, O88462
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3