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Protein

C-terminal-binding protein 2

Gene

Ctbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses (By similarity). Functions in brown adipose tissue (BAT) differentiation.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106NADBy similarity1
Binding sitei210NADBy similarity1
Active sitei272By similarity1
Binding sitei296NADBy similarity1
Active sitei301By similarity1
Active sitei321Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – 191NADBy similarity6
Nucleotide bindingi243 – 249NADBy similarity7
Nucleotide bindingi270 – 272NADBy similarity3
Nucleotide bindingi321 – 324NADBy similarity4

GO - Molecular functioni

  • chromatin binding Source: MGI
  • glyoxylate reductase (NADP) activity Source: GO_Central
  • hydroxypyruvate reductase activity Source: GO_Central
  • NAD binding Source: InterPro
  • protein-containing complex binding Source: MGI
  • protein homodimerization activity Source: GO_Central
  • protein kinase binding Source: MGI
  • retinoic acid receptor binding Source: MGI
  • transcription coactivator activity Source: MGI
  • transcription corepressor activity Source: MGI

GO - Biological processi

  • cellular response to leukemia inhibitory factor Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of chromatin binding Source: MGI
  • positive regulation of retinoic acid receptor signaling pathway Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • white fat cell differentiation Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase, Repressor
Biological processDifferentiation, Transcription, Transcription regulation
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-4641265 Repression of WNT target genes

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 2
Short name:
CtBP2
Gene namesi
Name:Ctbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1201686 Ctbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Nucleus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760451 – 445C-terminal-binding protein 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22Asymmetric dimethylarginineCombined sources1
Modified residuei428Phosphoserine; by HIPK2By similarity1

Post-translational modificationi

Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP56546
PeptideAtlasiP56546
PRIDEiP56546

2D gel databases

REPRODUCTION-2DPAGEiP56546

PTM databases

iPTMnetiP56546
PhosphoSitePlusiP56546

Expressioni

Tissue specificityi

Found in all tissues except spleen and liver.

Developmental stagei

Strong expression confined to the embryonic stages.

Gene expression databases

BgeeiENSMUSG00000030970
CleanExiMM_CTBP2
ExpressionAtlasiP56546 baseline and differential
GenevisibleiP56546 MM

Interactioni

Subunit structurei

Interacts with HIPK2 and PNN (By similarity). Interacts with the transcription factors ZNF217, BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Interacts also with the C-terminus of adenovirus E1A protein. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression. Interacts with MCRIP1 (By similarity).By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198962, 22 interactors
IntActiP56546, 10 interactors
MINTiP56546
STRINGi10090.ENSMUSP00000130294

Structurei

3D structure databases

ProteinModelPortaliP56546
SMRiP56546
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067 Eukaryota
COG0111 LUCA
GeneTreeiENSGT00530000063021
HOGENOMiHOG000136701
HOVERGENiHBG001898
InParanoidiP56546
KOiK04496
OMAiEDADIKC
OrthoDBiEOG091G08GS
PhylomeDBiP56546
TreeFamiTF313593

Family and domain databases

InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029753 D-isomer_DH_CS
IPR006140 D-isomer_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00671 D_2_HYDROXYACID_DH_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P56546-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI
60 70 80 90 100
LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV
110 120 130 140 150
IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTVC HILNLYRRNT
160 170 180 190 200
WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK
210 220 230 240 250
AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH
260 270 280 290 300
HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES
310 320 330 340 350
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP
360 370 380 390 400
ESLRNCVNKE FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP
410 420 430 440
PAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ
Length:445
Mass (Da):48,957
Last modified:December 1, 2000 - v2
Checksum:i5A81B9D75A9E493B
GO
Isoform 2 (identifier: P56546-2) [UniParc]FASTAAdd to basket
Also known as: Ribeye

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS

Show »
Length:988
Mass (Da):107,127
Checksum:iEF7CF59169C5A649
GO

Sequence cautioni

The sequence AAC40043 differs from that shown. Reason: Frameshift at position 12.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49P → H in AAC40043 (PubMed:9479502).Curated1
Sequence conflicti170E → D in AAC40043 (PubMed:9479502).Curated1
Sequence conflicti288G → A in AAC40043 (PubMed:9479502).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0276161 – 20MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGTWCELLNHRAQDTES YLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGMLGSRMPW EQVQGQLPALQDTGHLYPES GGKTVPHGQRTHGRAPSPGR YGREQPDTRLGIEVPTYSPN SSQVYNDICERPVDSTPARQ VAPTCLVVDPSSAVPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTMYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLA SSCRTEMQFRRQDRTGPLPR NRSSSFSHRSELLPNDMASL GTTALQFHPAGPLQTPSPRA GDLGRRPSSAPSQHLLETAA TYSTPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSAPLHSNPRT AYSTSYTVPMELLKRERSMT ASPLPSPHGSPQLLRKPGAA PVEPSTLPPVSQSLHTPHSP YQKVARRTGAPIIVSTMLTP EPS in isoform 2. CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016508 mRNA Translation: AAC40043.1 Frameshift.
AF059735 mRNA Translation: AAC33873.1
AB033123 mRNA Translation: BAA85181.1
AC119806 Genomic DNA No translation available.
CCDSiCCDS21930.1 [P56546-1]
CCDS52417.1 [P56546-2]
RefSeqiNP_001164215.1, NM_001170744.1 [P56546-2]
NP_034110.1, NM_009980.4 [P56546-1]
XP_006507371.1, XM_006507308.3 [P56546-1]
XP_006507372.1, XM_006507309.3 [P56546-1]
XP_006507373.1, XM_006507310.3 [P56546-1]
XP_006507374.1, XM_006507311.3 [P56546-1]
XP_006507375.1, XM_006507312.3 [P56546-1]
UniGeneiMm.246240
Mm.252063
Mm.389984

Genome annotation databases

EnsembliENSMUST00000033269; ENSMUSP00000033269; ENSMUSG00000030970 [P56546-1]
ENSMUST00000169570; ENSMUSP00000130294; ENSMUSG00000030970 [P56546-2]
GeneIDi13017
KEGGimmu:13017
UCSCiuc009kcy.1 mouse [P56546-1]
uc012fve.1 mouse [P56546-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCTBP2_MOUSE
AccessioniPrimary (citable) accession number: P56546
Secondary accession number(s): O54855, O88462
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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