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Protein

C-terminal-binding protein 2

Gene

Ctbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses (By similarity). Functions in brown adipose tissue (BAT) differentiation.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106NADBy similarity1
Binding sitei210NADBy similarity1
Active sitei272By similarity1
Binding sitei296NADBy similarity1
Active sitei301By similarity1
Active sitei321Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – 191NADBy similarity6
Nucleotide bindingi243 – 249NADBy similarity7
Nucleotide bindingi270 – 272NADBy similarity3
Nucleotide bindingi321 – 324NADBy similarity4

GO - Molecular functioni

  • chromatin binding Source: MGI
  • NAD binding Source: InterPro
  • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
  • protein homodimerization activity Source: GO_Central
  • retinoic acid receptor binding Source: MGI
  • transcription coactivator activity Source: MGI
  • transcription corepressor activity Source: MGI

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • positive regulation of chromatin binding Source: MGI
  • positive regulation of retinoic acid receptor signaling pathway Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • white fat cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 2
Short name:
CtBP2
Gene namesi
Name:Ctbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1201686. Ctbp2.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • nucleus Source: UniProtKB
  • ribbon synapse Source: MGI
  • synapse Source: MGI
  • transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Nucleus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760451 – 445C-terminal-binding protein 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22Asymmetric dimethylarginineCombined sources1
Modified residuei428Phosphoserine; by HIPK2By similarity1

Post-translational modificationi

Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiP56546.
PeptideAtlasiP56546.
PRIDEiP56546.

2D gel databases

REPRODUCTION-2DPAGEP56546.

PTM databases

iPTMnetiP56546.
PhosphoSitePlusiP56546.

Expressioni

Tissue specificityi

Found in all tissues except spleen and liver.

Developmental stagei

Strong expression confined to the embryonic stages.

Gene expression databases

BgeeiENSMUSG00000030970.
CleanExiMM_CTBP2.
ExpressionAtlasiP56546. baseline and differential.
GenevisibleiP56546. MM.

Interactioni

Subunit structurei

Interacts with HIPK2 and PNN (By similarity). Interacts with the transcription factors ZNF217, BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Interacts also with the C-terminus of adenovirus E1A protein. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression. Interacts with MCRIP1 (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HIC1Q145262EBI-1384883,EBI-2507362From a different organism.
MECOMQ031123EBI-1384883,EBI-1384862From a different organism.
PRDM16Q9HAZ2-22EBI-1384883,EBI-5282871From a different organism.
PRDM16Q9HAZ2-42EBI-1384883,EBI-4566658From a different organism.

GO - Molecular functioni

  • protein homodimerization activity Source: GO_Central
  • retinoic acid receptor binding Source: MGI

Protein-protein interaction databases

BioGridi198962. 20 interactors.
IntActiP56546. 7 interactors.
MINTiMINT-4084830.
STRINGi10090.ENSMUSP00000130294.

Structurei

3D structure databases

ProteinModelPortaliP56546.
SMRiP56546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOGENOMiHOG000136701.
HOVERGENiHBG001898.
InParanoidiP56546.
KOiK04496.
OMAiEDADIKC.
OrthoDBiEOG091G08GS.
PhylomeDBiP56546.
TreeFamiTF313593.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P56546-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI
60 70 80 90 100
LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV
110 120 130 140 150
IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTVC HILNLYRRNT
160 170 180 190 200
WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK
210 220 230 240 250
AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH
260 270 280 290 300
HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES
310 320 330 340 350
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP
360 370 380 390 400
ESLRNCVNKE FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP
410 420 430 440
PAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ
Length:445
Mass (Da):48,957
Last modified:December 1, 2000 - v2
Checksum:i5A81B9D75A9E493B
GO
Isoform 2 (identifier: P56546-2) [UniParc]FASTAAdd to basket
Also known as: Ribeye

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS

Show »
Length:988
Mass (Da):107,127
Checksum:iEF7CF59169C5A649
GO

Sequence cautioni

The sequence AAC40043 differs from that shown. Reason: Frameshift at position 12.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49P → H in AAC40043 (PubMed:9479502).Curated1
Sequence conflicti170E → D in AAC40043 (PubMed:9479502).Curated1
Sequence conflicti288G → A in AAC40043 (PubMed:9479502).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0276161 – 20MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGTWCELLNHRAQDTES YLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGMLGSRMPW EQVQGQLPALQDTGHLYPES GGKTVPHGQRTHGRAPSPGR YGREQPDTRLGIEVPTYSPN SSQVYNDICERPVDSTPARQ VAPTCLVVDPSSAVPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTMYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLA SSCRTEMQFRRQDRTGPLPR NRSSSFSHRSELLPNDMASL GTTALQFHPAGPLQTPSPRA GDLGRRPSSAPSQHLLETAA TYSTPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSAPLHSNPRT AYSTSYTVPMELLKRERSMT ASPLPSPHGSPQLLRKPGAA PVEPSTLPPVSQSLHTPHSP YQKVARRTGAPIIVSTMLTP EPS in isoform 2. CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016508 mRNA. Translation: AAC40043.1. Frameshift.
AF059735 mRNA. Translation: AAC33873.1.
AB033123 mRNA. Translation: BAA85181.1.
AC119806 Genomic DNA. No translation available.
CCDSiCCDS21930.1. [P56546-1]
CCDS52417.1. [P56546-2]
RefSeqiNP_001164215.1. NM_001170744.1. [P56546-2]
NP_034110.1. NM_009980.4. [P56546-1]
XP_006507371.1. XM_006507308.3. [P56546-1]
XP_006507372.1. XM_006507309.3. [P56546-1]
XP_006507373.1. XM_006507310.3. [P56546-1]
XP_006507374.1. XM_006507311.3. [P56546-1]
XP_006507375.1. XM_006507312.3. [P56546-1]
UniGeneiMm.246240.
Mm.252063.
Mm.389984.

Genome annotation databases

EnsembliENSMUST00000033269; ENSMUSP00000033269; ENSMUSG00000030970. [P56546-1]
ENSMUST00000169570; ENSMUSP00000130294; ENSMUSG00000030970. [P56546-2]
GeneIDi13017.
KEGGimmu:13017.
UCSCiuc009kcy.1. mouse. [P56546-1]
uc012fve.1. mouse. [P56546-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016508 mRNA. Translation: AAC40043.1. Frameshift.
AF059735 mRNA. Translation: AAC33873.1.
AB033123 mRNA. Translation: BAA85181.1.
AC119806 Genomic DNA. No translation available.
CCDSiCCDS21930.1. [P56546-1]
CCDS52417.1. [P56546-2]
RefSeqiNP_001164215.1. NM_001170744.1. [P56546-2]
NP_034110.1. NM_009980.4. [P56546-1]
XP_006507371.1. XM_006507308.3. [P56546-1]
XP_006507372.1. XM_006507309.3. [P56546-1]
XP_006507373.1. XM_006507310.3. [P56546-1]
XP_006507374.1. XM_006507311.3. [P56546-1]
XP_006507375.1. XM_006507312.3. [P56546-1]
UniGeneiMm.246240.
Mm.252063.
Mm.389984.

3D structure databases

ProteinModelPortaliP56546.
SMRiP56546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198962. 20 interactors.
IntActiP56546. 7 interactors.
MINTiMINT-4084830.
STRINGi10090.ENSMUSP00000130294.

PTM databases

iPTMnetiP56546.
PhosphoSitePlusiP56546.

2D gel databases

REPRODUCTION-2DPAGEP56546.

Proteomic databases

PaxDbiP56546.
PeptideAtlasiP56546.
PRIDEiP56546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033269; ENSMUSP00000033269; ENSMUSG00000030970. [P56546-1]
ENSMUST00000169570; ENSMUSP00000130294; ENSMUSG00000030970. [P56546-2]
GeneIDi13017.
KEGGimmu:13017.
UCSCiuc009kcy.1. mouse. [P56546-1]
uc012fve.1. mouse. [P56546-2]

Organism-specific databases

CTDi1488.
MGIiMGI:1201686. Ctbp2.

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOGENOMiHOG000136701.
HOVERGENiHBG001898.
InParanoidiP56546.
KOiK04496.
OMAiEDADIKC.
OrthoDBiEOG091G08GS.
PhylomeDBiP56546.
TreeFamiTF313593.

Miscellaneous databases

ChiTaRSiCtbp2. mouse.
PROiP56546.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030970.
CleanExiMM_CTBP2.
ExpressionAtlasiP56546. baseline and differential.
GenevisibleiP56546. MM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTBP2_MOUSE
AccessioniPrimary (citable) accession number: P56546
Secondary accession number(s): O54855, O88462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.