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P56546

- CTBP2_MOUSE

UniProt

P56546 - CTBP2_MOUSE

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Protein
C-terminal-binding protein 2
Gene
Ctbp2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses By similarity. Functions in brown adipose tissue (BAT) differentiation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei106 – 1061NAD By similarity
Binding sitei210 – 2101NAD By similarity
Active sitei272 – 2721 By similarity
Binding sitei296 – 2961NAD By similarity
Active sitei301 – 3011 By similarity
Active sitei321 – 3211Proton donor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi186 – 1916NAD By similarity
Nucleotide bindingi243 – 2497NAD By similarity
Nucleotide bindingi270 – 2723NAD By similarity
Nucleotide bindingi321 – 3244NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
  3. protein binding Source: UniProtKB
  4. transcription corepressor activity Source: MGI

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: UniProtKB
  2. transcription, DNA-templated Source: UniProtKB-KW
  3. white fat cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 2
Short name:
CtBP2
Gene namesi
Name:Ctbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1201686. Ctbp2.

Subcellular locationi

Nucleus Reviewed prediction. Cell junctionsynapse By similarity 1 Publication

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. nucleus Source: UniProtKB
  3. synapse Source: MGI
  4. transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Nucleus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445C-terminal-binding protein 2
PRO_0000076045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei428 – 4281Phosphoserine; by HIPK2 By similarity

Post-translational modificationi

Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56546.
PaxDbiP56546.
PRIDEiP56546.

2D gel databases

REPRODUCTION-2DPAGEP56546.

PTM databases

PhosphoSiteiP56546.

Expressioni

Tissue specificityi

Found in all tissues except spleen and liver.

Developmental stagei

Strong expression confined to the embryonic stages.

Gene expression databases

ArrayExpressiP56546.
BgeeiP56546.
CleanExiMM_CTBP2.
GenevestigatoriP56546.

Interactioni

Subunit structurei

Interacts with HIPK2 and PNN By similarity. Interacts with the transcription factors ZNF217, BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Interacts also with the C-terminus of adenovirus E1A protein. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HIC1Q145262EBI-1384883,EBI-2507362From a different organism.
MECOMQ031123EBI-1384883,EBI-1384862From a different organism.
PRDM16Q9HAZ2-22EBI-1384883,EBI-5282871From a different organism.
PRDM16Q9HAZ2-42EBI-1384883,EBI-4566658From a different organism.

Protein-protein interaction databases

BioGridi198962. 20 interactions.
IntActiP56546. 7 interactions.
MINTiMINT-4084830.

Structurei

3D structure databases

ProteinModelPortaliP56546.
SMRiP56546. Positions 33-362.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0111.
GeneTreeiENSGT00530000063021.
HOGENOMiHOG000136701.
HOVERGENiHBG001898.
InParanoidiP56546.
KOiK04496.
OMAiEDADIKC.
OrthoDBiEOG761BT9.
PhylomeDBiP56546.
TreeFamiTF313593.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P56546-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI    50
LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV 100
IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTVC HILNLYRRNT 150
WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK 200
AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH 250
HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES 300
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP 350
ESLRNCVNKE FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP 400
PAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ 445
Length:445
Mass (Da):48,957
Last modified:December 1, 2000 - v2
Checksum:i5A81B9D75A9E493B
GO
Isoform 2 (identifier: P56546-2) [UniParc]FASTAAdd to Basket

Also known as: Ribeye

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS

Show »
Length:988
Mass (Da):107,127
Checksum:iEF7CF59169C5A649
GO

Sequence cautioni

The sequence AAC40043.1 differs from that shown. Reason: Frameshift at position 12.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGTWCELLNHRAQDTES YLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGMLGSRMPW EQVQGQLPALQDTGHLYPES GGKTVPHGQRTHGRAPSPGR YGREQPDTRLGIEVPTYSPN SSQVYNDICERPVDSTPARQ VAPTCLVVDPSSAVPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTMYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLA SSCRTEMQFRRQDRTGPLPR NRSSSFSHRSELLPNDMASL GTTALQFHPAGPLQTPSPRA GDLGRRPSSAPSQHLLETAA TYSTPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSAPLHSNPRT AYSTSYTVPMELLKRERSMT ASPLPSPHGSPQLLRKPGAA PVEPSTLPPVSQSLHTPHSP YQKVARRTGAPIIVSTMLTP EPS in isoform 2.
VSP_027616Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491P → H in AAC40043. 1 Publication
Sequence conflicti170 – 1701E → D in AAC40043. 1 Publication
Sequence conflicti288 – 2881G → A in AAC40043. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF016508 mRNA. Translation: AAC40043.1. Frameshift.
AF059735 mRNA. Translation: AAC33873.1.
AB033123 mRNA. Translation: BAA85181.1.
AC119806 Genomic DNA. No translation available.
CCDSiCCDS21930.1. [P56546-1]
CCDS52417.1. [P56546-2]
RefSeqiNP_001164215.1. NM_001170744.1. [P56546-2]
NP_034110.1. NM_009980.4. [P56546-1]
XP_006507371.1. XM_006507308.1. [P56546-1]
XP_006507372.1. XM_006507309.1. [P56546-1]
XP_006507373.1. XM_006507310.1. [P56546-1]
XP_006507374.1. XM_006507311.1. [P56546-1]
XP_006507375.1. XM_006507312.1. [P56546-1]
XP_006507376.1. XM_006507313.1. [P56546-1]
UniGeneiMm.246240.
Mm.389984.

Genome annotation databases

EnsembliENSMUST00000033269; ENSMUSP00000033269; ENSMUSG00000030970. [P56546-1]
ENSMUST00000169570; ENSMUSP00000130294; ENSMUSG00000030970. [P56546-2]
GeneIDi13017.
KEGGimmu:13017.
UCSCiuc009kcy.1. mouse. [P56546-1]
uc012fve.1. mouse. [P56546-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF016508 mRNA. Translation: AAC40043.1 . Frameshift.
AF059735 mRNA. Translation: AAC33873.1 .
AB033123 mRNA. Translation: BAA85181.1 .
AC119806 Genomic DNA. No translation available.
CCDSi CCDS21930.1. [P56546-1 ]
CCDS52417.1. [P56546-2 ]
RefSeqi NP_001164215.1. NM_001170744.1. [P56546-2 ]
NP_034110.1. NM_009980.4. [P56546-1 ]
XP_006507371.1. XM_006507308.1. [P56546-1 ]
XP_006507372.1. XM_006507309.1. [P56546-1 ]
XP_006507373.1. XM_006507310.1. [P56546-1 ]
XP_006507374.1. XM_006507311.1. [P56546-1 ]
XP_006507375.1. XM_006507312.1. [P56546-1 ]
XP_006507376.1. XM_006507313.1. [P56546-1 ]
UniGenei Mm.246240.
Mm.389984.

3D structure databases

ProteinModelPortali P56546.
SMRi P56546. Positions 33-362.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198962. 20 interactions.
IntActi P56546. 7 interactions.
MINTi MINT-4084830.

PTM databases

PhosphoSitei P56546.

2D gel databases

REPRODUCTION-2DPAGE P56546.

Proteomic databases

MaxQBi P56546.
PaxDbi P56546.
PRIDEi P56546.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033269 ; ENSMUSP00000033269 ; ENSMUSG00000030970 . [P56546-1 ]
ENSMUST00000169570 ; ENSMUSP00000130294 ; ENSMUSG00000030970 . [P56546-2 ]
GeneIDi 13017.
KEGGi mmu:13017.
UCSCi uc009kcy.1. mouse. [P56546-1 ]
uc012fve.1. mouse. [P56546-2 ]

Organism-specific databases

CTDi 1488.
MGIi MGI:1201686. Ctbp2.

Phylogenomic databases

eggNOGi COG0111.
GeneTreei ENSGT00530000063021.
HOGENOMi HOG000136701.
HOVERGENi HBG001898.
InParanoidi P56546.
KOi K04496.
OMAi EDADIKC.
OrthoDBi EOG761BT9.
PhylomeDBi P56546.
TreeFami TF313593.

Miscellaneous databases

ChiTaRSi CTBP2. mouse.
NextBioi 282874.
PROi P56546.
SOURCEi Search...

Gene expression databases

ArrayExpressi P56546.
Bgeei P56546.
CleanExi MM_CTBP2.
Genevestigatori P56546.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view ]
PROSITEi PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3."
    Katsanis N., Fisher E.M.C.
    Genomics 47:294-299(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning and characterization of mCtBP2, a co-repressor that associates with basic krueppel-like factor and other mammalian transcriptional regulators."
    Turner J., Crossley M.
    EMBO J. 17:5129-5140(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1."
    Furusawa T., Moribe H., Kondoh H., Higashi Y.
    Mol. Cell. Biol. 19:8581-8590(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: ICR.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
    Strain: C57BL/6J.
  5. "Characterization of four autonomous repression domains in the corepressor receptor interacting protein 140."
    Christian M., Tullet J.M.A., Parker M.G.
    J. Biol. Chem. 279:15645-15651(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NRIP1.
  6. "Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP."
    Ueda J., Tachibana M., Ikura T., Shinkai Y.
    J. Biol. Chem. 281:20120-20128(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WIZ.
  7. "Specific recognition of ZNF217 and other zinc finger proteins at a surface groove of C-terminal binding proteins."
    Quinlan K.G.R., Nardini M., Verger A., Francescato P., Yaswen P., Corda D., Bolognesi M., Crossley M.
    Mol. Cell. Biol. 26:8159-8172(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZNF217.
  8. "Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex."
    Kajimura S., Seale P., Tomaru T., Erdjument-Bromage H., Cooper M.P., Ruas J.L., Chin S., Tempst P., Lazar M.A., Spiegelman B.M.
    Genes Dev. 22:1397-1409(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRDM16, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCTBP2_MOUSE
AccessioniPrimary (citable) accession number: P56546
Secondary accession number(s): O54855, O88462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi