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P56546 (CTBP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-terminal-binding protein 2
Short name=CtBP2
Gene names
Name:Ctbp2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses By similarity. Functions in brown adipose tissue (BAT) differentiation. Ref.8

Subunit structure

Interacts with HIPK2 and PNN By similarity. Interacts with the transcription factors ZNF217, BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Interacts also with the C-terminus of adenovirus E1A protein. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with NRIP1 and WIZ. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Nucleus Potential. Cell junction › synapse By similarity Ref.8.

Tissue specificity

Found in all tissues except spleen and liver.

Developmental stage

Strong expression confined to the embryonic stages.

Post-translational modification

Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-171 and Ser-177. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation By similarity.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Sequence caution

The sequence AAC40043.1 differs from that shown. Reason: Frameshift at position 12.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HIC1Q145262EBI-1384883,EBI-2507362From a different organism.
MECOMQ031123EBI-1384883,EBI-1384862From a different organism.
PRDM16Q9HAZ2-22EBI-1384883,EBI-5282871From a different organism.
PRDM16Q9HAZ2-42EBI-1384883,EBI-4566658From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P56546-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P56546-2)

Also known as: Ribeye;

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLTPEPS
Note: Contains a phosphothreonine at position 171 (By similarity). Contains a phosphoserine at position 177 (By similarity).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445C-terminal-binding protein 2
PRO_0000076045

Regions

Nucleotide binding186 – 1916NAD By similarity
Nucleotide binding243 – 2497NAD By similarity
Nucleotide binding270 – 2723NAD By similarity
Nucleotide binding321 – 3244NAD By similarity

Sites

Active site2721 By similarity
Active site3011 By similarity
Active site3211Proton donor By similarity
Binding site1061NAD By similarity
Binding site2101NAD By similarity
Binding site2961NAD By similarity

Amino acid modifications

Modified residue4281Phosphoserine; by HIPK2 By similarity

Natural variations

Alternative sequence1 – 2020MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGTWCELLNHRAQDTES YLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGMLGSRMPW EQVQGQLPALQDTGHLYPES GGKTVPHGQRTHGRAPSPGR YGREQPDTRLGIEVPTYSPN SSQVYNDICERPVDSTPARQ VAPTCLVVDPSSAVPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTMYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLA SSCRTEMQFRRQDRTGPLPR NRSSSFSHRSELLPNDMASL GTTALQFHPAGPLQTPSPRA GDLGRRPSSAPSQHLLETAA TYSTPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSAPLHSNPRT AYSTSYTVPMELLKRERSMT ASPLPSPHGSPQLLRKPGAA PVEPSTLPPVSQSLHTPHSP YQKVARRTGAPIIVSTMLTP EPS in isoform 2.
VSP_027616

Experimental info

Sequence conflict491P → H in AAC40043. Ref.1
Sequence conflict1701E → D in AAC40043. Ref.1
Sequence conflict2881G → A in AAC40043. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 5A81B9D75A9E493B

FASTA44548,957
        10         20         30         40         50         60 
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI LKDLATVAFC 

        70         80         90        100        110        120 
DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV IVRIGSGYDN VDIKAAGELG 

       130        140        150        160        170        180 
IAVCNIPSAA VEETADSTVC HILNLYRRNT WLYQALREGT RVQSVEQIRE VASGAARIRG 

       190        200        210        220        230        240 
ETLGLIGFGR TGQAVAVRAK AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS 

       250        260        270        280        290        300 
LHCNLNEHNH HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES 

       310        320        330        340        350        360 
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP ESLRNCVNKE 

       370        380        390        400        410        420 
FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP PAMEGIIPGG IPVTHNLPTV 

       430        440 
AHPSQAPSPN QPTKHGDNRE HPNEQ

« Hide

Isoform 2 (Ribeye) [UniParc].

Checksum: EF7CF59169C5A649
Show »

FASTA988107,127

References

« Hide 'large scale' references
[1]"A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3."
Katsanis N., Fisher E.M.C.
Genomics 47:294-299(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning and characterization of mCtBP2, a co-repressor that associates with basic krueppel-like factor and other mammalian transcriptional regulators."
Turner J., Crossley M.
EMBO J. 17:5129-5140(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1."
Furusawa T., Moribe H., Kondoh H., Higashi Y.
Mol. Cell. Biol. 19:8581-8590(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: ICR.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2).
Strain: C57BL/6J.
[5]"Characterization of four autonomous repression domains in the corepressor receptor interacting protein 140."
Christian M., Tullet J.M.A., Parker M.G.
J. Biol. Chem. 279:15645-15651(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NRIP1.
[6]"Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP."
Ueda J., Tachibana M., Ikura T., Shinkai Y.
J. Biol. Chem. 281:20120-20128(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH WIZ.
[7]"Specific recognition of ZNF217 and other zinc finger proteins at a surface groove of C-terminal binding proteins."
Quinlan K.G.R., Nardini M., Verger A., Francescato P., Yaswen P., Corda D., Bolognesi M., Crossley M.
Mol. Cell. Biol. 26:8159-8172(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZNF217.
[8]"Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex."
Kajimura S., Seale P., Tomaru T., Erdjument-Bromage H., Cooper M.P., Ruas J.L., Chin S., Tempst P., Lazar M.A., Spiegelman B.M.
Genes Dev. 22:1397-1409(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PRDM16, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF016508 mRNA. Translation: AAC40043.1. Frameshift.
AF059735 mRNA. Translation: AAC33873.1.
AB033123 mRNA. Translation: BAA85181.1.
AC119806 Genomic DNA. No translation available.
IPIIPI00114237.
IPI00856974.
RefSeqNP_001164215.1. NM_001170744.1.
NP_034110.1. NM_009980.4.
UniGeneMm.246240.
Mm.389984.

3D structure databases

ProteinModelPortalP56546.
SMRP56546. Positions 33-362.
ModBaseSearch...

Protein-protein interaction databases

IntActP56546. 6 interactions.

PTM databases

PhosphoSiteP56546.

2D gel databases

REPRODUCTION-2DPAGEP56546.

Proteomic databases

PaxDbP56546.
PRIDEP56546.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033269; ENSMUSP00000033269; ENSMUSG00000030970.
ENSMUST00000169570; ENSMUSP00000130294; ENSMUSG00000030970.
GeneID13017.
KEGGmmu:13017.
UCSCuc012fve.1. mouse.

Organism-specific databases

CTD1488.
MGIMGI:1201686. Ctbp2.

Phylogenomic databases

eggNOGCOG0111.
GeneTreeENSGT00530000063021.
HOGENOMHOG000136701.
HOVERGENHBG001898.
InParanoidP56546.
KOK04496.
OMAMEDADIK.
OrthoDBEOG4HHP2H.

Gene expression databases

ArrayExpressP56546.
BgeeP56546.
CleanExMM_CTBP2.
GenevestigatorP56546.
GermOnlineENSMUSG00000030970. Mus musculus.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. False negative.
PS00670. D_2_HYDROXYACID_DH_2. False negative.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCTBP2. mouse.
NextBio282874.
SOURCESearch...

Entry information

Entry nameCTBP2_MOUSE
AccessionPrimary (citable) accession number: P56546
Secondary accession number(s): O54855, O88462
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 1, 2000
Last modified: May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents