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Protein

C-terminal-binding protein 2

Gene

CTBP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity).By similarity
Isoform 2 probably acts as a scaffold for specialized synapses.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106NADBy similarity1
Binding sitei210NAD1 Publication1
Active sitei272By similarity1
Binding sitei296NAD1 Publication1
Active sitei301By similarity1
Active sitei321Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – 191NAD1 Publication6
Nucleotide bindingi243 – 249NAD1 Publication7
Nucleotide bindingi270 – 272NAD1 Publication3
Nucleotide bindingi321 – 324NAD1 Publication4

GO - Molecular functioni

GO - Biological processi

  • cellular response to leukemia inhibitory factor Source: Ensembl
  • negative regulation of cell proliferation Source: ProtInc
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of chromatin binding Source: Ensembl
  • positive regulation of retinoic acid receptor signaling pathway Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • viral genome replication Source: ProtInc
  • white fat cell differentiation Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase, Repressor
Biological processDifferentiation, Host-virus interaction, Transcription, Transcription regulation
LigandNAD

Enzyme and pathway databases

ReactomeiR-HSA-4641265. Repression of WNT target genes.
R-HSA-5339700. TCF7L2 mutants don't bind CTBP.
SignaLinkiP56545.
SIGNORiP56545.

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 2
Short name:
CtBP2
Gene namesi
Name:CTBP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000175029.16.
HGNCiHGNC:2495. CTBP2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Nucleus, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi1488.
OpenTargetsiENSG00000175029.
PharmGKBiPA26996.

Chemistry databases

ChEMBLiCHEMBL3797016.

Polymorphism and mutation databases

BioMutaiCTBP2.
DMDMi3182976.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760441 – 445C-terminal-binding protein 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22Asymmetric dimethylarginineCombined sources1
Modified residuei428PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP56545.
MaxQBiP56545.
PaxDbiP56545.
PeptideAtlasiP56545.
PRIDEiP56545.

PTM databases

iPTMnetiP56545.
PhosphoSitePlusiP56545.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

Gene expression databases

BgeeiENSG00000175029.
CleanExiHS_CTBP2.
ExpressionAtlasiP56545. baseline and differential.
GenevisibleiP56545. HS.

Organism-specific databases

HPAiCAB031916.
HPA023559.
HPA023564.
HPA044971.

Interactioni

Subunit structurei

Interacts with the C-terminus of adenovirus E1A protein. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with HIPK2 and ZNF217. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression (By similarity). Interacts with PNN, NRIP1 and WIZ. Interacts with human adenovirus 5 E1A protein; this interaction seems to potentiate viral replication (PubMed:23747199). Interacts with MCRIP1 (PubMed:25728771).By similarity6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein complex binding Source: CAFA
  • protein homodimerization activity Source: GO_Central
  • protein kinase binding Source: CAFA
  • retinoic acid receptor binding Source: Ensembl

Protein-protein interaction databases

BioGridi107870. 109 interactors.
CORUMiP56545.
DIPiDIP-42104N.
IntActiP56545. 104 interactors.
MINTiMINT-1188878.
STRINGi9606.ENSP00000311825.

Chemistry databases

BindingDBiP56545.

Structurei

Secondary structure

1445
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 38Combined sources4
Helixi47 – 51Combined sources5
Turni52 – 54Combined sources3
Beta strandi56 – 59Combined sources4
Helixi65 – 67Combined sources3
Helixi70 – 75Combined sources6
Beta strandi76 – 81Combined sources6
Beta strandi83 – 85Combined sources3
Helixi89 – 93Combined sources5
Beta strandi100 – 106Combined sources7
Helixi113 – 118Combined sources6
Beta strandi122 – 124Combined sources3
Beta strandi128 – 130Combined sources3
Helixi131 – 147Combined sources17
Helixi149 – 157Combined sources9
Helixi165 – 171Combined sources7
Turni172 – 174Combined sources3
Beta strandi182 – 186Combined sources5
Helixi190 – 199Combined sources10
Helixi200 – 202Combined sources3
Beta strandi205 – 209Combined sources5
Helixi217 – 220Combined sources4
Helixi229 – 235Combined sources7
Beta strandi237 – 241Combined sources5
Helixi255 – 258Combined sources4
Beta strandi265 – 269Combined sources5
Helixi273 – 275Combined sources3
Helixi278 – 286Combined sources9
Beta strandi289 – 296Combined sources8
Beta strandi299 – 302Combined sources4
Turni309 – 312Combined sources4
Beta strandi314 – 318Combined sources5
Helixi327 – 346Combined sources20
Turni349 – 352Combined sources4
Beta strandi354 – 356Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OMEX-ray2.80A/B/C/D/E/F/G/H31-364[»]
4LCJX-ray2.86A/B/C/D/E/F/G/H31-362[»]
ProteinModelPortaliP56545.
SMRiP56545.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56545.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
HOVERGENiHBG001898.
InParanoidiP56545.
KOiK04496.
OrthoDBiEOG091G08GS.
PhylomeDBiP56545.
TreeFamiTF313593.

Family and domain databases

InterProiView protein in InterPro
IPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P56545-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI
60 70 80 90 100
LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV
110 120 130 140 150
IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTIC HILNLYRRNT
160 170 180 190 200
WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK
210 220 230 240 250
AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH
260 270 280 290 300
HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES
310 320 330 340 350
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP
360 370 380 390 400
ESLRNCVNKE FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP
410 420 430 440
AAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ
Length:445
Mass (Da):48,945
Last modified:July 15, 1998 - v1
Checksum:i0A8C21CEB36807FA
GO
Isoform 2 (identifier: P56545-2) [UniParc]FASTAAdd to basket
Also known as: Ribeye

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MALVDKHKVKRQRLDRICEG → MPVPSRHINI...VSTMLAPEPS

Show »
Length:985
Mass (Da):106,187
Checksum:i90EC841907295622
GO
Isoform 3 (identifier: P56545-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MALVDKHKVKRQRLDRICE → MRPGLPGPTG...LPVHSYRSWA

Show »
Length:513
Mass (Da):56,102
Checksum:iA7A54EB7F0F34922
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87L → F in AAG45951 (PubMed:11163272).Curated1
Sequence conflicti88T → N in AAG45951 (PubMed:11163272).Curated1
Sequence conflicti112D → N in AAG45951 (PubMed:11163272).Curated1
Sequence conflicti292G → R in AAH37900 (PubMed:15489334).Curated1
Sequence conflicti411I → T in AAH47018 (PubMed:15489334).Curated1
Isoform 2 (identifier: P56545-2)
Sequence conflicti455Y → H in AAG45951 (PubMed:11163272).Curated1
Sequence conflicti539Q → E in AAG45951 (PubMed:11163272).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0276151 – 20MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAESLRPLGRRSS LTYGTAEGTWFEPNHRPQDA ALPVAAEPYLYREAVYNSVA ARKGSTPDFTFYDSRQAVMS GRSPLLPREYYSDPSGAARV PKEPPLYRDPGVSRPVPSYG VLGSRTSWDPMQGRSPALQD AGHLYRDPGGKMIPQGRQTQ SRAASPGRYGREQPDTRYGA EVPAYPLSQVFSDISERPID PAPARQVAPTCLVVDPSSAA APEGSTGVAPGALNRGYGPA RESIPSKMAYETYEADLSTF QGPGGKRTVLPEFLAFLRAE GLAEATLGALLQQGFDSPAV LATLEDADIKSVAPNLGQAR VLSRLANSCRTEMQLRRQDR GGPLPRARSSSFSHRSELLH GDLASLGAAAPLQTASPRAG DPARRPSSAPSQHLLETAAT YSAPGVGTHAPHFPSNSGYS SPTPCALTARLSPTYPLQAG VALTNPGPSNPLHPGPRTAY STAYTVPMELLKRERNVAAS PLPSPHGSPQVLRKPGAPLG PSTLPPASQSLHTPHSPYQK VARRTGAPIIVSTMLAPEPS in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_0589461 – 19MALVD…DRICE → MRPGLPGPTGLCAQTSSRGQ KSVLKQKESCGIWQLYHFLS RKQEPRWEPCVSGSSSGDGA VADLADELRGYPALCCTLPV HSYRSWA in isoform 3. Add BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016507 mRNA. Translation: AAC39603.1.
AF222711 mRNA. Translation: AAG45951.1.
BT007012 mRNA. Translation: AAP35658.1.
AK290390 mRNA. Translation: BAF83079.1.
AL833398 mRNA. Translation: CAH10590.1.
AL596261 Genomic DNA. No translation available.
AL731571 Genomic DNA. No translation available.
CH471066 Genomic DNA. Translation: EAW49247.1.
CH471066 Genomic DNA. Translation: EAW49250.1.
CH471066 Genomic DNA. Translation: EAW49249.1.
BC002486 mRNA. Translation: AAH02486.1.
BC037900 mRNA. Translation: AAH37900.1.
BC047018 mRNA. Translation: AAH47018.1.
BC052276 mRNA. Translation: AAH52276.1.
BC072020 mRNA. Translation: AAH72020.1.
CCDSiCCDS7643.1. [P56545-1]
CCDS7644.1. [P56545-2]
RefSeqiNP_001077383.1. NM_001083914.2. [P56545-1]
NP_001277143.1. NM_001290214.2. [P56545-1]
NP_001277144.1. NM_001290215.2. [P56545-1]
NP_001307941.1. NM_001321012.1. [P56545-1]
NP_001307942.1. NM_001321013.1. [P56545-1]
NP_001307943.1. NM_001321014.1. [P56545-1]
NP_001320.1. NM_001329.3. [P56545-1]
NP_073713.2. NM_022802.2. [P56545-2]
XP_005269618.1. XM_005269561.2. [P56545-1]
XP_005269621.1. XM_005269564.2. [P56545-1]
XP_005269624.1. XM_005269567.2. [P56545-1]
XP_005269625.1. XM_005269568.4. [P56545-1]
XP_005269626.1. XM_005269569.2. [P56545-1]
XP_005269628.1. XM_005269571.2. [P56545-1]
XP_005269629.1. XM_005269572.3. [P56545-1]
XP_006717705.1. XM_006717642.2. [P56545-1]
XP_011537653.1. XM_011539351.1. [P56545-1]
XP_011537655.1. XM_011539353.1. [P56545-1]
XP_011537656.1. XM_011539354.1. [P56545-1]
XP_011537657.1. XM_011539355.1. [P56545-1]
XP_016871245.1. XM_017015756.1. [P56545-1]
XP_016871246.1. XM_017015757.1. [P56545-1]
UniGeneiHs.501345.

Genome annotation databases

EnsembliENST00000309035; ENSP00000311825; ENSG00000175029. [P56545-2]
ENST00000334808; ENSP00000357816; ENSG00000175029. [P56545-3]
ENST00000337195; ENSP00000338615; ENSG00000175029. [P56545-1]
ENST00000411419; ENSP00000410474; ENSG00000175029. [P56545-1]
ENST00000494626; ENSP00000436285; ENSG00000175029. [P56545-1]
ENST00000531469; ENSP00000434630; ENSG00000175029. [P56545-1]
GeneIDi1488.
KEGGihsa:1488.
UCSCiuc001lie.5. human. [P56545-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCTBP2_HUMAN
AccessioniPrimary (citable) accession number: P56545
Secondary accession number(s): A8K2X5
, D3DRF5, O43449, Q5SQP7, Q69YI3, Q86SV0, Q8IY44, Q9H2T8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 22, 2017
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families