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Protein

Deoxyribonuclease-2-alpha

Gene

Dnase2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.2 Publications

Miscellaneous

Not required for the generation of the characteristic DNA fragmentation observed in apoptotic cells, but for the degradation of DNA from dying cells.

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei297By similarity1

GO - Molecular functioni

  • deoxyribonuclease II activity Source: MGI

GO - Biological processi

  • apoptotic DNA fragmentation Source: GO_Central
  • DNA catabolic process Source: MGI
  • DNA catabolic process, endonucleolytic Source: MGI
  • erythrocyte differentiation Source: MGI

Keywordsi

Molecular functionDevelopmental protein, Endonuclease, Hydrolase, Nuclease
Biological processApoptosis

Enzyme and pathway databases

BRENDAi3.1.22.1 3474
ReactomeiR-MMU-432720 Lysosome Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-2-alpha (EC:3.1.22.1)
Alternative name(s):
Acid DNase
Deoxyribonuclease II alpha
Short name:
DNase II alpha
Lysosomal DNase II
Gene namesi
Name:Dnase2
Synonyms:Dnase2a, Dnl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1329019 Dnase2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Absence of Dnase2 is a cause of severe fetal anemia and of perinatal lethality due to malformation of the diaphragm.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000729220 – 353Deoxyribonuclease-2-alphaAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 161Sequence analysis
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi88N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi214N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi268N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi269 ↔ 349Sequence analysis
Disulfide bondi310 ↔ 329Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP56542
PeptideAtlasiP56542
PRIDEiP56542

PTM databases

iPTMnetiP56542
PhosphoSitePlusiP56542

Expressioni

Tissue specificityi

Highly expressed in fetal liver macrophages.1 Publication

Gene expression databases

BgeeiENSMUSG00000003812
CleanExiMM_DNASE2A
ExpressionAtlasiP56542 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003910

Structurei

3D structure databases

ProteinModelPortaliP56542
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase II family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3825 Eukaryota
ENOG4111G8U LUCA
GeneTreeiENSGT00390000002634
HOGENOMiHOG000261682
HOVERGENiHBG051387
InParanoidiP56542
KOiK01158
OMAiLQVQFWQ
OrthoDBiEOG091G08N7
PhylomeDBiP56542
TreeFamiTF314536

Family and domain databases

InterProiView protein in InterPro
IPR004947 DNase_II
PANTHERiPTHR10858 PTHR10858, 1 hit
PfamiView protein in Pfam
PF03265 DNase_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLRSLLLA ALLWVPAEAL SCYGDSGQPV DWFVVYKLPA HSGSRDTPKG
60 70 80 90 100
LTYKYMDQNS DGWQDGVGYI NSSEGAVGRS LQPLYRKNSS QLAFLLYNDQ
110 120 130 140 150
PPKSSSARDS TGHGHTKGVL LLDQEGGFWL VHSVPRFPPP ASSGAYTWPP
160 170 180 190 200
NAQTFGQTLL CVSLPFTQFA RIGKQLTYTY PLVYDHKLEG FFAQKLPDLE
210 220 230 240 250
TVIKNQHVLH EPWNSSVILT SQAGATFQSF AKFGKFGDDL YSGWLAEALG
260 270 280 290 300
TNLQVQFWQN SPGILPSNCS GAYQVLDVTQ TGFPGPSRLT FSATEDHSKW
310 320 330 340 350
CVAPQGPWAC VGDMNRNKAE THRGGGTVCT QLPSFWKAFQ SLVKDWKPCI

EGS
Length:353
Mass (Da):38,811
Last modified:July 15, 1998 - v1
Checksum:iF53CDD81CF6FFCF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045741 mRNA Translation: AAC35750.1
AF190459 Genomic DNA Translation: AAF20386.1
BC058609 mRNA Translation: AAH58609.1
BC060661 mRNA Translation: AAH60661.1
CCDSiCCDS22484.1
RefSeqiNP_034192.1, NM_010062.3
UniGeneiMm.350831

Genome annotation databases

EnsembliENSMUST00000003910; ENSMUSP00000003910; ENSMUSG00000003812
GeneIDi13423
KEGGimmu:13423
UCSCiuc009moa.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDNS2A_MOUSE
AccessioniPrimary (citable) accession number: P56542
Secondary accession number(s): O55053
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 25, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health