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Protein

Deoxyribonuclease-2-alpha

Gene

Dnase2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.2 Publications

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei297 – 2971By similarity

GO - Molecular functioni

  • deoxyribonuclease II activity Source: MGI

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • DNA catabolic process Source: MGI
  • DNA catabolic process, endonucleolytic Source: MGI
  • erythrocyte differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BRENDAi3.1.22.1. 3474.
ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-2-alpha (EC:3.1.22.1)
Alternative name(s):
Acid DNase
Deoxyribonuclease II alpha
Short name:
DNase II alpha
Lysosomal DNase II
Gene namesi
Name:Dnase2
Synonyms:Dnase2a, Dnl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1329019. Dnase2a.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • lysosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Absence of Dnase2 is a cause of severe fetal anemia and of perinatal lethality due to malformation of the diaphragm.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 353334Deoxyribonuclease-2-alphaPRO_0000007292Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi22 ↔ 161Sequence analysis
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence analysis
Glycosylationi88 – 881N-linked (GlcNAc...)By similarity
Glycosylationi214 – 2141N-linked (GlcNAc...)By similarity
Glycosylationi268 – 2681N-linked (GlcNAc...)By similarity
Disulfide bondi269 ↔ 349Sequence analysis
Disulfide bondi310 ↔ 329Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP56542.
PRIDEiP56542.

PTM databases

iPTMnetiP56542.
PhosphoSiteiP56542.

Expressioni

Tissue specificityi

Highly expressed in fetal liver macrophages.1 Publication

Gene expression databases

BgeeiP56542.
CleanExiMM_DNASE2A.
ExpressionAtlasiP56542. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003910.

Structurei

3D structure databases

ProteinModelPortaliP56542.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase II family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiP56542.
KOiK01158.
OMAiLQVQFWQ.
OrthoDBiEOG7F5122.
PhylomeDBiP56542.
TreeFamiTF314536.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLRSLLLA ALLWVPAEAL SCYGDSGQPV DWFVVYKLPA HSGSRDTPKG
60 70 80 90 100
LTYKYMDQNS DGWQDGVGYI NSSEGAVGRS LQPLYRKNSS QLAFLLYNDQ
110 120 130 140 150
PPKSSSARDS TGHGHTKGVL LLDQEGGFWL VHSVPRFPPP ASSGAYTWPP
160 170 180 190 200
NAQTFGQTLL CVSLPFTQFA RIGKQLTYTY PLVYDHKLEG FFAQKLPDLE
210 220 230 240 250
TVIKNQHVLH EPWNSSVILT SQAGATFQSF AKFGKFGDDL YSGWLAEALG
260 270 280 290 300
TNLQVQFWQN SPGILPSNCS GAYQVLDVTQ TGFPGPSRLT FSATEDHSKW
310 320 330 340 350
CVAPQGPWAC VGDMNRNKAE THRGGGTVCT QLPSFWKAFQ SLVKDWKPCI

EGS
Length:353
Mass (Da):38,811
Last modified:July 15, 1998 - v1
Checksum:iF53CDD81CF6FFCF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045741 mRNA. Translation: AAC35750.1.
AF190459 Genomic DNA. Translation: AAF20386.1.
BC058609 mRNA. Translation: AAH58609.1.
BC060661 mRNA. Translation: AAH60661.1.
CCDSiCCDS22484.1.
RefSeqiNP_034192.1. NM_010062.3.
UniGeneiMm.350831.

Genome annotation databases

EnsembliENSMUST00000003910; ENSMUSP00000003910; ENSMUSG00000003812.
GeneIDi13423.
KEGGimmu:13423.
UCSCiuc009moa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045741 mRNA. Translation: AAC35750.1.
AF190459 Genomic DNA. Translation: AAF20386.1.
BC058609 mRNA. Translation: AAH58609.1.
BC060661 mRNA. Translation: AAH60661.1.
CCDSiCCDS22484.1.
RefSeqiNP_034192.1. NM_010062.3.
UniGeneiMm.350831.

3D structure databases

ProteinModelPortaliP56542.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003910.

PTM databases

iPTMnetiP56542.
PhosphoSiteiP56542.

Proteomic databases

PaxDbiP56542.
PRIDEiP56542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003910; ENSMUSP00000003910; ENSMUSG00000003812.
GeneIDi13423.
KEGGimmu:13423.
UCSCiuc009moa.1. mouse.

Organism-specific databases

CTDi13423.
MGIiMGI:1329019. Dnase2a.

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiP56542.
KOiK01158.
OMAiLQVQFWQ.
OrthoDBiEOG7F5122.
PhylomeDBiP56542.
TreeFamiTF314536.

Enzyme and pathway databases

BRENDAi3.1.22.1. 3474.
ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.

Miscellaneous databases

PROiP56542.
SOURCEiSearch...

Gene expression databases

BgeeiP56542.
CleanExiMM_DNASE2A.
ExpressionAtlasiP56542. baseline and differential.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of human and murine DNase II."
    Baker K.P., Baron W.F., Henzel W.J., Spencer S.A.
    Gene 215:281-289(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Deoxyribonuclease II: structure and chromosomal localization of the murine gene, and comparison with the genomic structure of the human and three C. elegans homologs."
    Krieser R.J., Eastman A.
    Gene 252:155-162(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver."
    Kawane K., Fukuyama H., Kondoh G., Takeda J., Ohsawa Y., Uchiyama Y., Nagata S.
    Science 292:1546-1549(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INVOLVEMENT IN FATAL ANEMIA.
  5. "Deoxyribonuclease IIalpha is required during the phagocytic phase of apoptosis and its loss causes perinatal lethality."
    Krieser R.J., MacLea K.S., Longnecker D.S., Fields J.L., Fiering S., Eastman A.
    Cell Death Differ. 9:956-962(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INVOLVEMENT IN PERINATAL LETHALITY.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiDNS2A_MOUSE
AccessioniPrimary (citable) accession number: P56542
Secondary accession number(s): O55053
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Not required for the generation of the characteristic DNA fragmentation observed in apoptotic cells, but for the degradation of DNA from dying cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.