P56527 (APHA_PROMI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Class B acid phosphatase
Short name=CBAP
EC=3.1.3.2

Gene names

Name:	aphA
Synonyms:	napA

Organism

Proteus mirabilis

Taxonomic identifier

584 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	160 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.
Sequence caution	The sequence M11587 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	acid phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – ›160	›137	Class B acid phosphatase		PRO_0000024007
Regions
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	69	1	Nucleophile By similarity
	Active site	71	1	Proton donor By similarity
	Metal binding	69	1	Magnesium By similarity
	Metal binding	71	1	Magnesium; via carbonyl oxygen By similarity
Experimental info
	Non-terminal residue	160	1

Sequences

	Sequence		Length	Mass (Da)
	P56527 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 395541C45A456735	FASTA	160	18,081
	10 20 30 40 50 60 MRKVTLTLSA IALALSLNGA AMAKVHMPEV VSQGVTVTEL AHQQPIKWVS VAEIEKSLEG 70 80 90 100 110 120 QAPMAVGFDI DDTFWFSSPG FYRVKLEYSP NDFSYLKNPE FWEKMNNEWD KFSMPKQVGI 130 140 150 160 DLVQMHLKRG DTVYFITGRT QTKTETCVTK YVQEGLNIPA

References

[1]	"Nucleotide sequence analysis of the cat gene of Proteus mirabilis: comparison with the type I (Tn9) cat gene." Charles I.G., Keyte J.W., Shaw W.V. J. Bacteriol. 164:123-129(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and characterization of the NapA acid phosphatase/phosphotransferase of Morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes." Thaller M.C., Lombardi G., Berlutti F., Schippa S., Rossolini G.M. Microbiology 141:147-154(1995) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, CONCEPTUAL TRANSLATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M11587 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	P56527.
SMR	P56527. Positions 31-160.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR005519. Acid_phosphat_B. IPR023214. HAD-like_dom. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_PROMI

Accession

Primary (citable) accession number: P56527

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program