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Protein

Class B acid phosphatase

Gene

aphA

Organism
Proteus mirabilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69NucleophileBy similarity1
Metal bindingi69MagnesiumBy similarity1
Active sitei71Proton donorBy similarity1
Metal bindingi71Magnesium; via carbonyl oxygenBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.2By similarity)
Short name:
CBAP
Gene namesi
Name:aphA
Synonyms:napA
OrganismiProteus mirabilis
Taxonomic identifieri584 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaeProteus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000002400724 – ›160Class B acid phosphataseAdd BLAST›137

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi529507.PMI0729.

Structurei

3D structure databases

ProteinModelPortaliP56527.
SMRiP56527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 138Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56527-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKVTLTLSA IALALSLNGA AMAKVHMPEV VSQGVTVTEL AHQQPIKWVS
60 70 80 90 100
VAEIEKSLEG QAPMAVGFDI DDTFWFSSPG FYRVKLEYSP NDFSYLKNPE
110 120 130 140 150
FWEKMNNEWD KFSMPKQVGI DLVQMHLKRG DTVYFITGRT QTKTETCVTK
160
YVQEGLNIPA
Length:160
Mass (Da):18,081
Last modified:July 15, 1998 - v1
Checksum:i395541C45A456735
GO

Sequence cautioni

The sequence M11587 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1601

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11587 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11587 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortaliP56527.
SMRiP56527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi529507.PMI0729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105F0S. Bacteria.
COG3700. LUCA.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B-like.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPHA_PROMI
AccessioniPrimary (citable) accession number: P56527
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.