Reviewed, UniProtKB/Swiss-Prot P56527 (APHA_PROMI)

Names and origin

Protein names

Recommended name:
    Class B acid phosphatase
    EC=3.1.3.2

Gene names

Name:	aphA
Synonyms:	napA

Organism

Proteus mirabilis

Taxonomic identifier

584 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	160 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.
Sequence caution	The sequence M11587 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – ›160	›137	Class B acid phosphatase		PRO_0000024007
Sites
	Metal binding	69	1	Magnesium By similarity
	Metal binding	71	1	Magnesium; via carbonyl oxygen By similarity
Experimental info
	Non-terminal residue	160	1

Sequences

	Sequence		Length	Mass (Da)
	P56527-1 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 395541C45A456735	FASTA	160	18,081
	10 20 30 40 50 60 MRKVTLTLSA IALALSLNGA AMAKVHMPEV VSQGVTVTEL AHQQPIKWVS VAEIEKSLEG 70 80 90 100 110 120 QAPMAVGFDI DDTFWFSSPG FYRVKLEYSP NDFSYLKNPE FWEKMNNEWD KFSMPKQVGI 130 140 150 160 DLVQMHLKRG DTVYFITGRT QTKTETCVTK YVQEGLNIPA

References

[1]	"Nucleotide sequence analysis of the cat gene of Proteus mirabilis: comparison with the type I (Tn9) cat gene." Charles I.G., Keyte J.W., Shaw W.V. J. Bacteriol. 164:123-129(1985) [PubMed: 3900035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and characterization of the NapA acid phosphatase/phosphotransferase of Morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes." Thaller M.C., Lombardi G., Berlutti F., Schippa S., Rossolini G.M. Microbiology 141:147-154(1995) [PubMed: 7894706] [Abstract] Cited for: IDENTIFICATION, CONCEPTUAL TRANSLATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M11587 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.2. 639.
Family and domain databases
InterPro	IPR005519. Acid_phosphat_B. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_PROMI

Accession

Primary (citable) accession number: P56527

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)