Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-glucosidase

Gene

aglA

Organism
Aspergillus niger
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes malto-oligosaccharides, but has a low activity toward soluble starch.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei490Nucleophile1
Active sitei493By similarity1
Active sitei660Proton donorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.20 518

Protein family/group databases

CAZyiGH31 Glycoside Hydrolase Family 31
mycoCLAPiAGL31A_ASPNG

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Maltase
Gene namesi
Name:aglA
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3435

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000001857626 – 985Alpha-glucosidaseAdd BLAST960

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36O-linked (Man) threonine1 Publication1
Glycosylationi124N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi143N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi218N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi347N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi422N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi506N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi534N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi537N-linked (GlcNAc...) asparagine1
Glycosylationi545O-linked (Man) serine1 Publication1
Glycosylationi550O-linked (Man) serine1 Publication1
Glycosylationi559O-linked (Man) threonine1 Publication1
Glycosylationi560O-linked (Man) serine1 Publication1
Glycosylationi561O-linked (Man) threonine1 Publication1
Glycosylationi562O-linked (Man) serine1 Publication1
Glycosylationi571O-linked (Man) threonine1 Publication1
Glycosylationi601N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi623N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi835N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi881N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi895O-linked (Man) serine1 Publication1
Glycosylationi899N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi957N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi970N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

The O-linked saccharide is not identified, but is probably mannose.1 Publication

Keywords - PTMi

Glycoprotein

PTM databases

GlyConnecti43
iPTMnetiP56526
UniCarbKBiP56526

Interactioni

Chemistry databases

BindingDBiP56526

Structurei

3D structure databases

ProteinModelPortaliP56526
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1065 Eukaryota
COG1501 LUCA

Family and domain databases

Gene3Di2.60.40.1180, 2 hits
InterProiView protein in InterPro
IPR031727 Gal_mutarotase_N
IPR011013 Gal_mutarotase_sf_dom
IPR000322 Glyco_hydro_31
IPR030458 Glyco_hydro_31_AS
IPR030459 Glyco_hydro_31_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF01055 Glyco_hydro_31, 1 hit
PF16863 NtCtMGAM_N, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF74650 SSF74650, 2 hits
PROSITEiView protein in PROSITE
PS00129 GLYCOSYL_HYDROL_F31_1, 1 hit
PS00707 GLYCOSYL_HYDROL_F31_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLTHLLAR AWLVPLAYGA SQSLLSTTAP SQPQFTIPAS ADVGAQLIAN
60 70 80 90 100
IDDPQAADAQ SVCPGYKASK VQHNSRGFTA SLQLAGRPCN VYGTDVESLT
110 120 130 140 150
LSVEYQDSDR LNIQILPTHV DSTNASWYFL SENLVPRPKA SLNASVSQSD
160 170 180 190 200
LFVSWSNEPS FNFKVIRKAT GDALFSTEGT VLVYENQFIE FVTALPEEYN
210 220 230 240 250
LYGLGEHITQ FRLQRNANLT IYPSDDGTPI DQNLYGQHPF YLDTRYYKGD
260 270 280 290 300
RQNGSYIPVK SSEADASQDY ISLSHGVFLR NSHGLEILLR SQKLIWRTLG
310 320 330 340 350
GGIDLTFYSG PAPADVTRQY LTSTVGLPAM QQYNTLGFHQ CRWGYNNWSD
360 370 380 390 400
LADVVANFEK FEIPLEYIWT DIDYMHGYRN FDNDQHRFSY SEGDEFLSKL
410 420 430 440 450
HESGRYYVPI VDAALYIPNP ENASDAYATY DRGAADDVFL KNPDGSLYIG
460 470 480 490 500
AVWPGYTVFP DWHHPKAVDF WANELVIWSK KVAFDGVWYD MSEVSSFCVG
510 520 530 540 550
SCGTGNLTLN PAHPSFLLPG EPGDIIYDYP EAFNITNATE AASASAGASS
560 570 580 590 600
QAAATATTTS TSVSYLRTTP TPGVRNVEHP PYVINHDQEG HDLSVHAVSP
610 620 630 640 650
NATHVDGVEE YDVHGLYGHQ GLNATYQGLL EVWSHKRRPF IIGRSTFAGS
660 670 680 690 700
GKWAGHWGGD NYSKWWSMYY SISQALSFSL FGIPMFGADT CGFNGNSDEE
710 720 730 740 750
LCNRWMQLSA FFPFYRNHNE LSTIPQEPYR WASVIEATKS AMRIRYAILP
760 770 780 790 800
YFYTLFDLAH TTGSTVMRAL SWEFPNDPTL AAVETQFMVG PAIMVVPVLE
810 820 830 840 850
PLVNTVKGVF PGVGHGEVWY DWYTQAAVDA KPGVNTTISA PLGHIPVYVR
860 870 880 890 900
GGNILPMQEP ALTTREARQT PWALLAALGS NGTASGQLYL DDGESIYPNA
910 920 930 940 950
TLHVDFTASR SSLRSSAQGR WKERNPLANV TVLGVNKEPS AVTLNGQAVF
960 970 980
PGSVTYNSTS QVLFVGGLQN LTKGGAWAEN WVLEW
Length:985
Mass (Da):108,913
Last modified:July 15, 1998 - v1
Checksum:i9A18772AEB2E0927
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti27 – 28TT → LL in strain: GN-8. 2
Natural varianti42D → A in strain: GN-8. 1
Natural varianti929N → M in strain: GN-8. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45356 Genomic DNA Translation: BAA23616.1
PIRiJC1199
JC1200
JC5561

Similar proteinsi

Entry informationi

Entry nameiAGLU_ASPNG
AccessioniPrimary (citable) accession number: P56526
Secondary accession number(s): O13451
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 25, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health