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Protein

NADPH:adrenodoxin oxidoreductase, mitochondrial

Gene

Fdxr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.

Catalytic activityi

2 reduced adrenodoxin + NADP+ = 2 oxidized adrenodoxin + NADPH.

Cofactori

Pathwayi: cholesterol metabolism

This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the pathway cholesterol metabolism and in Steroid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511FAD; via amide nitrogenBy similarity
Binding sitei72 – 721FADBy similarity
Binding sitei80 – 801FAD; via amide nitrogenBy similarity
Binding sitei116 – 1161FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei243 – 2431NADPBy similarity
Binding sitei401 – 4011FAD; via amide nitrogenBy similarity
Binding sitei408 – 4081NADP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi187 – 1904NADPBy similarity
Nucleotide bindingi231 – 2322NADPBy similarity
Nucleotide bindingi408 – 4103FADBy similarity

GO - Molecular functioni

  • NADPH-adrenodoxin reductase activity Source: RGD
  • NADPH binding Source: RGD

GO - Biological processi

  • cholesterol metabolic process Source: UniProtKB-UniPathway
  • NADPH oxidation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Electron transport, Lipid metabolism, Steroid metabolism, Sterol metabolism, Transport

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14306.
ReactomeiR-RNO-196108. Pregnenolone biosynthesis.
R-RNO-211976. Endogenous sterols.
R-RNO-2395516. Electron transport from NADPH to Ferredoxin.
UniPathwayiUPA00296.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH:adrenodoxin oxidoreductase, mitochondrial (EC:1.18.1.6)
Short name:
AR
Short name:
Adrenodoxin reductase
Alternative name(s):
Ferredoxin--NADP(+) reductase
Short name:
Ferredoxin reductase
Gene namesi
Name:Fdxr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621648. Fdxr.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionAdd
BLAST
Chaini35 – 494460NADPH:adrenodoxin oxidoreductase, mitochondrialPRO_0000019422Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei313 – 3131PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP56522.
PRIDEiP56522.

PTM databases

iPTMnetiP56522.
PhosphoSiteiP56522.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003387.
GenevisibleiP56522. RN.

Interactioni

Subunit structurei

Monomer. Interacts directly with FDX1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004592.

Structurei

3D structure databases

ProteinModelPortaliP56522.
SMRiP56522. Positions 40-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1800. Eukaryota.
COG0493. LUCA.
GeneTreeiENSGT00390000013574.
HOGENOMiHOG000249250.
HOVERGENiHBG002132.
InParanoidiP56522.
KOiK18914.
OMAiMAPRCWR.
OrthoDBiEOG091G0EIW.
PhylomeDBiP56522.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR021163. Ferredox_Rdtase_adrenod.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000362. FNR. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRCWRWWS WSAWPGVRPL PSRSTPTPGF CKKFSTQETT PQICVVGSGP
60 70 80 90 100
AGFYTAQHLL KHHTRAHVDI YEKQLVPFGL VRFGVAPDHP EVKNVINTFT
110 120 130 140 150
QTARSDRCAF RGNVVVGRDV SVPELREAYH AVVLSYGAED HQPLEIPGEE
160 170 180 190 200
LPGVVSARAF VGWYNGLPEN QKLAPDLSCD TAVILGQGNV ALDVARILLT
210 220 230 240 250
PPEHLEKTDI TEVALGVLRQ SRVKTVWIVG RRGPLQVAFT IKELREMIQL
260 270 280 290 300
PGTQPILDPS DFLGLQDRIK DVPRPRKRLT ELLLRTATEK PGVEEAARRA
310 320 330 340 350
LASRAWGLRF FRSPQQVLPT PDGRRVAGIR LAVTRLEGVG ESTRAVPTGD
360 370 380 390 400
VEDLPCGLLL SSVGYKSRPI DPSVPFDPKL GIIPNTEGRV VNAPGLYCSG
410 420 430 440 450
WVKRGPTGVI TTTMTDSFLT SQVLLKDLKA GLLPSGPRPG YTAIQALLSD
460 470 480 490
RGVRPVSFSD WEKLDAEEVA RGQGTGKPRE KLVDRREMLQ LLGH
Length:494
Mass (Da):54,363
Last modified:July 15, 1998 - v1
Checksum:i5F07B37DFAA9525D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63761 mRNA. Translation: BAA23759.1.
BC088844 mRNA. Translation: AAH88844.1.
RefSeqiNP_077067.1. NM_024153.1.
UniGeneiRn.10860.

Genome annotation databases

EnsembliENSRNOT00000085636; ENSRNOP00000075248; ENSRNOG00000058497.
GeneIDi79122.
KEGGirno:79122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63761 mRNA. Translation: BAA23759.1.
BC088844 mRNA. Translation: AAH88844.1.
RefSeqiNP_077067.1. NM_024153.1.
UniGeneiRn.10860.

3D structure databases

ProteinModelPortaliP56522.
SMRiP56522. Positions 40-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004592.

PTM databases

iPTMnetiP56522.
PhosphoSiteiP56522.

Proteomic databases

PaxDbiP56522.
PRIDEiP56522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000085636; ENSRNOP00000075248; ENSRNOG00000058497.
GeneIDi79122.
KEGGirno:79122.

Organism-specific databases

CTDi2232.
RGDi621648. Fdxr.

Phylogenomic databases

eggNOGiKOG1800. Eukaryota.
COG0493. LUCA.
GeneTreeiENSGT00390000013574.
HOGENOMiHOG000249250.
HOVERGENiHBG002132.
InParanoidiP56522.
KOiK18914.
OMAiMAPRCWR.
OrthoDBiEOG091G0EIW.
PhylomeDBiP56522.

Enzyme and pathway databases

UniPathwayiUPA00296.
BioCyciMetaCyc:MONOMER-14306.
ReactomeiR-RNO-196108. Pregnenolone biosynthesis.
R-RNO-211976. Endogenous sterols.
R-RNO-2395516. Electron transport from NADPH to Ferredoxin.

Miscellaneous databases

PROiP56522.

Gene expression databases

BgeeiENSRNOG00000003387.
GenevisibleiP56522. RN.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
3.50.50.60. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR021163. Ferredox_Rdtase_adrenod.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000362. FNR. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADRO_RAT
AccessioniPrimary (citable) accession number: P56522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.