Reviewed, UniProtKB/Swiss-Prot P56522 (ADRO_RAT)

Names and origin

Protein names

Recommended name:
    NADPH:adrenodoxin oxidoreductase, mitochondrial
      Short name=Adrenodoxin reductase
      Short name=AR
    EC=1.18.1.2
Alternative name(s):
    Ferredoxin--NADP(+) reductase
      Short name=Ferredoxin reductase

Gene names

Name:

Fdxr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD.
Pathway	Steroid metabolism; cholesterol metabolism.
Subunit structure	Monomer. Interacts directly with FDX1 By similarity.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Electron transport Lipid metabolism Steroid metabolism Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADPH-adrenodoxin reductase activity Ref.1 Inferred from direct assay. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion
	Chain	35 – 494	460	NADPH:adrenodoxin oxidoreductase, mitochondrial		PRO_0000019422
Regions
	Nucleotide binding	187 – 190	4	NADP By similarity
	Nucleotide binding	231 – 232	2	NADP By similarity
	Nucleotide binding	408 – 410	3	FAD By similarity
Sites
	Binding site	51	1	FAD; via amide nitrogen By similarity
	Binding site	72	1	FAD By similarity
	Binding site	80	1	FAD; via amide nitrogen By similarity
	Binding site	116	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	243	1	NADP By similarity
	Binding site	401	1	FAD; via amide nitrogen By similarity
	Binding site	408	1	NADP; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56522-1 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 5F07B37DFAA9525D	FASTA	494	54,363
	10 20 30 40 50 60 MAPRCWRWWS WSAWPGVRPL PSRSTPTPGF CKKFSTQETT PQICVVGSGP AGFYTAQHLL 70 80 90 100 110 120 KHHTRAHVDI YEKQLVPFGL VRFGVAPDHP EVKNVINTFT QTARSDRCAF RGNVVVGRDV 130 140 150 160 170 180 SVPELREAYH AVVLSYGAED HQPLEIPGEE LPGVVSARAF VGWYNGLPEN QKLAPDLSCD 190 200 210 220 230 240 TAVILGQGNV ALDVARILLT PPEHLEKTDI TEVALGVLRQ SRVKTVWIVG RRGPLQVAFT 250 260 270 280 290 300 IKELREMIQL PGTQPILDPS DFLGLQDRIK DVPRPRKRLT ELLLRTATEK PGVEEAARRA 310 320 330 340 350 360 LASRAWGLRF FRSPQQVLPT PDGRRVAGIR LAVTRLEGVG ESTRAVPTGD VEDLPCGLLL 370 380 390 400 410 420 SSVGYKSRPI DPSVPFDPKL GIIPNTEGRV VNAPGLYCSG WVKRGPTGVI TTTMTDSFLT 430 440 450 460 470 480 SQVLLKDLKA GLLPSGPRPG YTAIQALLSD RGVRPVSFSD WEKLDAEEVA RGQGTGKPRE 490 KLVDRREMLQ LLGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, overproduction and characterization of rat adrenodoxin reductase." Sagara Y., Watanabe Y., Kodama H., Aramaki H. Biochim. Biophys. Acta 1434:284-295(1999) [PubMed: 10525147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 34-54. Strain: Wistar. Tissue: Adrenal gland.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D63761 mRNA. Translation: BAA23759.1. BC088844 mRNA. Translation: AAH88844.1.
IPI	IPI00193379.
RefSeq	NP_077067.1.
UniGene	Rn.10860
3D structure databases
HSSP	HSSP built from PDB template 1CJC based on UniProtKB P08165.
SMR	P56522. Positions 40-494.
ModBase	Search...
Protein-protein interaction databases
STRING	P56522.
Genome annotation databases
Ensembl	ENSRNOT00000004592; ENSRNOP00000004592; ENSRNOG00000003387; Rattus norvegicus. [Genome view]
GeneID	79122.
KEGG	rno:79122.
Organism-specific databases
CTD	79122.
RGD	621648. Fdxr.
Phylogenomic databases
HOVERGEN	P56522.
Enzyme and pathway databases
BRENDA	1.18.1.2. 248.
Gene expression databases
ArrayExpress	P56522.
Genevestigator	P56522.
GermOnline	ENSRNOG00000003387. Rattus norvegicus.
Family and domain databases
InterPro	IPR000759. Adrndx_reductase. IPR013027. FAD_pyr_nucl-diS_OxRdtase. [Graphical view]
Pfam	PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00419. ADXRDTASE.
ProtoNet	Search...
Other Resources
NextBio	614546.

Entry information

Entry name

ADRO_RAT

Accession

Primary (citable) accession number: P56522

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	October 13, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)