Reviewed, UniProtKB/Swiss-Prot P56512 (LDH1_LACPL)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 1
      Short name=L-LDH 1
    EC=1.1.1.27

Gene names

Name:	ldh1
Synonyms:	l-ldhL, ldh, ldhL, ldhL1
Ordered Locus Names:	lp_0537

Organism

Lactobacillus plantarum [Complete proteome] [HAMAP]

Taxonomic identifier

1590 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	L-lactate dehydrogenase 1		PRO_0000168357
Regions
	Nucleotide binding	16 – 44	29	NAD By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	124	1	NAD or substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	54	1	D → H in CAA50277. Ref.1
	Sequence conflict	245	1	K → Q in BAD16691. Ref.2
	Sequence conflict	302	1	S → C in CAA50277. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P56512-1 [UniParc]. Last modified March 25, 2003. Version 2. Checksum: 34B8A5FD12A64EBC	FASTA	320	34,206
	10 20 30 40 50 60 MSSMPNHQKV VLVGDGAVGS SYAFAMAQQG IAEEFVIVDV VKDRTKGDAL DLEDAQAFTA 70 80 90 100 110 120 PKKIYSGEYS DCKDADLVVI TAGAPQKPGE SRLDLVNKNL NILSSIVKPV VDSGFDGIFL 130 140 150 160 170 180 VAANPVDILT YATWKFSGFP KDRVIGSGTS LDSSRLRVAL GKQFNVDPRS VDAYIMGEHG 190 200 210 220 230 240 DSEFAAYSTA TIGTRPVRDV AKEQGVSDED LAKLEDGVRN KAYDIINLKG ATFYGIGTAL 250 260 270 280 290 300 MRISKAILRD ENAVLPVGAY MDGQYGLNDI YIGTPAVIGG TGLKQIIESP LSADELKKMQ 310 320 DSAATLKKVL NDGLAELENK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lactobacillus plantarum ldhL gene: overexpression and deletion." Ferain T., Garmyn D., Bernard N., Hols P., Delcour J. J. Bacteriol. 176:596-601(1994) [PubMed: 8300514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DG301.
[2]	"Expression of Derf 7 gene in Lactobacillus plantarum." Kim J., Tsutsui M., Yamashita M., Murooka Y. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: L137.
[3]	"Complete genome sequence of Lactobacillus plantarum WCFS1." Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P., Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J., Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M., Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M., Siezen R.J. Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003) [PubMed: 12566566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-793 / NCIMB 8826 / WCFS1.
[4]	"Expression of LDH gene in Candida kefyr isolated from Mongolian Koumiss." Ham J., Kim H., Kim H. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2-320.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70926 Genomic DNA. Translation: CAA50277.1. AB177763 Genomic DNA. Translation: BAD16691.1. Different initiation. AL935253 Genomic DNA. Translation: CAD63155.1. AF548372 Genomic DNA. Translation: AAN40797.1.
PIR	A36957.
RefSeq	NP_784314.1.
3D structure databases
HSSP	HSSP built from PDB template 1EZ4 based on UniProtKB P56511.
SMR	P56512. Positions 3-320.
ModBase	Search...
Genome annotation databases
GeneID	1061886.
GenomeReviews	Gene locus lp_0537 in contig AL935263_GR.
KEGG	lpl:lp_0537.
NMPDR	fig|220668.1.peg.448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P56512.
Enzyme and pathway databases
BioCyc	LPLA220668:LP_0537-MON.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_LACPL

Accession

Primary (citable) accession number: P56512
Secondary accession number(s): P26299, Q8GMW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	March 25, 2003
Last modified:	November 25, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)