P56500 (UCP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial uncoupling protein 2
Short name=UCP 2

Alternative name(s):
Solute carrier family 25 member 8

Gene names

Name:	Ucp2
Synonyms:	Slc25a8

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat By similarity.
Subunit structure	Acts as a dimer forming a proton channel By similarity.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in a variety of organs, with predominant expression in the heart, lung and spleen.
Sequence similarities	Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification] Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 19041646. Source: RGD cellular response to amino acid starvation Inferred from expression pattern PubMed 18692019. Source: RGD cellular response to glucose stimulus Inferred from expression pattern PubMed 18692019. Source: RGD cellular response to insulin stimulus Inferred from expression pattern PubMed 18079609. Source: RGD female pregnancy Inferred from expression pattern PubMed 20393169. Source: RGD liver regeneration Inferred from expression pattern PubMed 19104935. Source: RGD mitochondrial transport Inferred from direct assay PubMed 12372827. Source: RGD negative regulation of apoptotic process Inferred from mutant phenotype PubMed 18703058. Source: RGD negative regulation of insulin secretion involved in cellular response to glucose stimulus Inferred from mutant phenotype PubMed 21172424. Source: RGD positive regulation of cell death Inferred from mutant phenotype PubMed 19361538. Source: RGD response to fatty acid Inferred from expression pattern PubMed 18079609. Source: RGD response to hypoxia Inferred from expression pattern PubMed 17704287. Source: RGD response to superoxide Inferred from direct assay PubMed 12372827. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 12372827. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Mitochondrial uncoupling protein 2		PRO_0000090667
Regions
	Topological domain	1 – 10	10	Mitochondrial matrix Potential
	Transmembrane	11 – 32	22	Helical; Name=1; Potential
	Topological domain	33 – 77	45	Mitochondrial intermembrane Potential
	Transmembrane	78 – 100	23	Helical; Name=2; Potential
	Topological domain	101 – 119	19	Mitochondrial matrix Potential
	Transmembrane	120 – 136	17	Helical; Name=3; Potential
	Topological domain	137 – 180	44	Mitochondrial intermembrane Potential
	Transmembrane	181 – 197	17	Helical; Name=4; Potential
	Topological domain	198 – 214	17	Mitochondrial matrix Potential
	Transmembrane	215 – 234	20	Helical; Name=5; Potential
	Topological domain	235 – 268	34	Mitochondrial intermembrane Potential
	Transmembrane	269 – 291	23	Helical; Name=6; Potential
	Topological domain	292 – 309	18	Mitochondrial matrix Potential
	Repeat	11 – 106	96	Solcar 1
	Repeat	114 – 203	90	Solcar 2
	Repeat	212 – 297	86	Solcar 3
	Region	276 – 298	23	Purine nucleotide binding By similarity
Experimental info
	Sequence conflict	9	1	V → L in BAA28832. Ref.4
	Sequence conflict	268	1	A → T in BAA25698. Ref.3
	Sequence conflict	268	1	A → T in AAH62230. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P56500 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 3297935CF997AA0E	FASTA	309	33,377
	10 20 30 40 50 60 MVGFKATDVP PTATVKFLGA GTAACIADLI TFPLDTAKVR LQIQGESQGL ARTAASAQYR 70 80 90 100 110 120 GVLGTILTMV RTEGPRSLYN GLVAGLQRQM SFASVRIGLY DSVKQFYTKG SEHAGIGSRL 130 140 150 160 170 180 LAGSTTGALA VAVAQPTDVV KVRFQAQARA GGGRRYQSTV EAYKTIAREE GIRGLWKGTS 190 200 210 220 230 240 PNVARNAIVN CTELVTYDLI KDTLLKANLM TDDLPCHFTS AFGAGFCTTV IASPVDVVKT 250 260 270 280 290 300 RYMNSALGQY HSAGHCALTM LRKEGPRAFY KGFMPSFLRL GSWNVVMFVT YEQLKRALMA AYESREAPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Strobel A., Strosberg A.D., Issad T. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: White adipose tissue.
[2]	"Cloning of rat uncoupling protein-3 and uncoupling protein-2 cDNAs: their gene expression in rats fed high-fat diet." Matsuda J., Hosoda K., Itoh H., Son C., Doi K., Tanaka T., Fukunaga Y., Inoue G., Nishimura H., Yoshimasa Y., Yamori Y., Nakao K. FEBS Lett. 418:200-204(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brown adipose tissue.
[3]	Yamazaki K., Yoshitomi H., Tanaka I. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Spleen.
[4]	"Molecular cloning of rat uncoupling protein 2 cDNA and its expression in genetically obese Zucker fatty (fa/fa) rats." Hidaka S., Kakuma T., Yoshimatsu H., Yasunaga S., Kurokawa M., Sakata T. Biochim. Biophys. Acta 1389:178-186(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF039033 mRNA. Translation: AAC98733.1. AB006613 mRNA. Translation: BAA23383.1. AB010743 mRNA. Translation: BAA25698.1. AB005143 mRNA. Translation: BAA28832.1. BC062230 mRNA. Translation: AAH62230.1.
IPI	IPI00324565.
RefSeq	NP_062227.2. NM_019354.2.
UniGene	Rn.13333.
3D structure databases
ProteinModelPortal	P56500.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000024156.
Proteomic databases
PaxDb	P56500.
PRIDE	P56500.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000024156; ENSRNOP00000024156; ENSRNOG00000017854.
GeneID	54315.
KEGG	rno:54315.
UCSC	RGD:3932. rat.
Organism-specific databases
CTD	7351.
RGD	3932. Ucp2.
Phylogenomic databases
eggNOG	NOG300436.
GeneTree	ENSGT00550000074223.
HOGENOM	HOG000165140.
HOVERGEN	HBG009528.
InParanoid	P56500.
KO	K15103.
OMA	PCSLYNG.
OrthoDB	EOG4V6ZH7.
Gene expression databases
Genevestigator	P56500.
GermOnline	ENSRNOG00000017854. Rattus norvegicus.
Family and domain databases
Gene3D	1.50.40.10. 1 hit.
InterPro	IPR002030. Mit_uncoupling. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00784. MTUNCOUPLING.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	610992.

Entry information

Entry name

UCP2_RAT

Accession

Primary (citable) accession number: P56500
Secondary accession number(s): O70178, O88183, Q6GST1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program