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P56500 (UCP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial uncoupling protein 2

Short name=UCP 2
Alternative name(s):
Solute carrier family 25 member 8
Gene names
Name:Ucp2
Synonyms:Slc25a8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat By similarity.

Subunit structure

Acts as a dimer forming a proton channel By similarity.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in a variety of organs, with predominant expression in the heart, lung and spleen.

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from expression pattern PubMed 19041646. Source: RGD

cellular response to amino acid starvation

Inferred from expression pattern PubMed 18692019. Source: RGD

cellular response to glucose stimulus

Inferred from expression pattern PubMed 18692019. Source: RGD

cellular response to hormone stimulus

Inferred from expression pattern PubMed 18079609. Source: RGD

cellular response to insulin stimulus

Inferred from expression pattern PubMed 18079609. Source: RGD

female pregnancy

Inferred from expression pattern PubMed 20393169. Source: RGD

liver regeneration

Inferred from expression pattern PubMed 19104935. Source: RGD

mitochondrial transport

Inferred from direct assay PubMed 12372827. Source: RGD

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 18703058. Source: RGD

negative regulation of insulin secretion involved in cellular response to glucose stimulus

Inferred from mutant phenotype PubMed 21172424. Source: RGD

positive regulation of cell death

Inferred from mutant phenotype PubMed 19361538. Source: RGD

response to fatty acid

Inferred from expression pattern PubMed 18079609. Source: RGD

response to glucose

Inferred from expression pattern PubMed 18079609. Source: RGD

response to hypoxia

Inferred from expression pattern PubMed 17704287. Source: RGD

response to superoxide

Inferred from direct assay PubMed 12372827. Source: RGD

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

mitochondrial inner membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 12372827. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Mitochondrial uncoupling protein 2
PRO_0000090667

Regions

Topological domain1 – 1010Mitochondrial matrix Potential
Transmembrane11 – 3222Helical; Name=1; Potential
Topological domain33 – 7745Mitochondrial intermembrane Potential
Transmembrane78 – 10023Helical; Name=2; Potential
Topological domain101 – 11919Mitochondrial matrix Potential
Transmembrane120 – 13617Helical; Name=3; Potential
Topological domain137 – 18044Mitochondrial intermembrane Potential
Transmembrane181 – 19717Helical; Name=4; Potential
Topological domain198 – 21417Mitochondrial matrix Potential
Transmembrane215 – 23420Helical; Name=5; Potential
Topological domain235 – 26834Mitochondrial intermembrane Potential
Transmembrane269 – 29123Helical; Name=6; Potential
Topological domain292 – 30918Mitochondrial matrix Potential
Repeat11 – 10696Solcar 1
Repeat114 – 20390Solcar 2
Repeat212 – 29786Solcar 3
Region276 – 29823Purine nucleotide binding By similarity

Experimental info

Sequence conflict91V → L in BAA28832. Ref.4
Sequence conflict2681A → T in BAA25698. Ref.3
Sequence conflict2681A → T in AAH62230. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P56500 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 3297935CF997AA0E

FASTA30933,377
        10         20         30         40         50         60 
MVGFKATDVP PTATVKFLGA GTAACIADLI TFPLDTAKVR LQIQGESQGL ARTAASAQYR 

        70         80         90        100        110        120 
GVLGTILTMV RTEGPRSLYN GLVAGLQRQM SFASVRIGLY DSVKQFYTKG SEHAGIGSRL 

       130        140        150        160        170        180 
LAGSTTGALA VAVAQPTDVV KVRFQAQARA GGGRRYQSTV EAYKTIAREE GIRGLWKGTS 

       190        200        210        220        230        240 
PNVARNAIVN CTELVTYDLI KDTLLKANLM TDDLPCHFTS AFGAGFCTTV IASPVDVVKT 

       250        260        270        280        290        300 
RYMNSALGQY HSAGHCALTM LRKEGPRAFY KGFMPSFLRL GSWNVVMFVT YEQLKRALMA 


AYESREAPF 

« Hide

References

« Hide 'large scale' references
[1]Strobel A., Strosberg A.D., Issad T.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: White adipose tissue.
[2]"Cloning of rat uncoupling protein-3 and uncoupling protein-2 cDNAs: their gene expression in rats fed high-fat diet."
Matsuda J., Hosoda K., Itoh H., Son C., Doi K., Tanaka T., Fukunaga Y., Inoue G., Nishimura H., Yoshimasa Y., Yamori Y., Nakao K.
FEBS Lett. 418:200-204(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brown adipose tissue.
[3]Yamazaki K., Yoshitomi H., Tanaka I.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Spleen.
[4]"Molecular cloning of rat uncoupling protein 2 cDNA and its expression in genetically obese Zucker fatty (fa/fa) rats."
Hidaka S., Kakuma T., Yoshimatsu H., Yasunaga S., Kurokawa M., Sakata T.
Biochim. Biophys. Acta 1389:178-186(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Brain.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pituitary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039033 mRNA. Translation: AAC98733.1.
AB006613 mRNA. Translation: BAA23383.1.
AB010743 mRNA. Translation: BAA25698.1.
AB005143 mRNA. Translation: BAA28832.1.
BC062230 mRNA. Translation: AAH62230.1.
RefSeqNP_062227.2. NM_019354.2.
XP_006229846.1. XM_006229784.1.
UniGeneRn.13333.

3D structure databases

ProteinModelPortalP56500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000024156.

Proteomic databases

PaxDbP56500.
PRIDEP56500.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000024156; ENSRNOP00000024156; ENSRNOG00000017854.
GeneID54315.
KEGGrno:54315.
UCSCRGD:3932. rat.

Organism-specific databases

CTD7351.
RGD3932. Ucp2.

Phylogenomic databases

eggNOGNOG300436.
GeneTreeENSGT00750000117384.
HOGENOMHOG000165140.
HOVERGENHBG009528.
InParanoidP56500.
KOK15103.
OMASPNIARN.
OrthoDBEOG793B7Z.
PhylomeDBP56500.
TreeFamTF323211.

Gene expression databases

GenevestigatorP56500.

Family and domain databases

Gene3D1.50.40.10. 1 hit.
InterProIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSPR00784. MTUNCOUPLING.
SUPFAMSSF103506. SSF103506. 1 hit.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio610992.
PROP56500.

Entry information

Entry nameUCP2_RAT
AccessionPrimary (citable) accession number: P56500
Secondary accession number(s): O70178, O88183, Q6GST1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families