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Protein

Interferon regulatory factor 5

Gene

Irf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi14 – 122109IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 5
Short name:
IRF-5
Gene namesi
Name:Irf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1350924. Irf5.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Shuttles between the nucleus and the cytoplasm.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Interferon regulatory factor 5PRO_0000154559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571Phosphoserine; by TBK1By similarity
Modified residuei300 – 3001PhosphoserineBy similarity
Cross-linki410 – 410Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki411 – 411Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei434 – 4341PhosphoserineBy similarity
Modified residuei436 – 4361PhosphoserineBy similarity
Modified residuei439 – 4391PhosphoserineBy similarity
Modified residuei445 – 4451PhosphoserineBy similarity

Post-translational modificationi

'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.1 Publication
Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP56477.
PRIDEiP56477.

PTM databases

iPTMnetiP56477.
PhosphoSiteiP56477.

Expressioni

Gene expression databases

BgeeiP56477.
CleanExiMM_IRF5.
ExpressionAtlasiP56477. baseline and differential.
GenevisibleiP56477. MM.

Interactioni

Subunit structurei

Homodimer, when phosphorylated.By similarity

Protein-protein interaction databases

BioGridi205111. 4 interactions.
DIPiDIP-49382N.
IntActiP56477. 2 interactions.
MINTiMINT-1565953.
STRINGi10090.ENSMUSP00000004392.

Structurei

3D structure databases

ProteinModelPortaliP56477.
SMRiP56477. Positions 19-119, 231-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 187Nuclear localization signalBy similarity
Motifi149 – 15911Nuclear export signalBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 124Poly-Pro
Compositional biasi140 – 1489Poly-Glu

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFCV. Eukaryota.
ENOG410XRXT. LUCA.
HOGENOMiHOG000037433.
HOVERGENiHBG105715.
InParanoidiP56477.
KOiK09446.
OMAiYDGPRDM.
OrthoDBiEOG7CCBR1.
PhylomeDBiP56477.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR029838. IRF5.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11949:SF10. PTHR11949:SF10. 1 hit.
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
SM01243. IRF-3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHSAPGIPP PPRRVRLKPW LVAQVNSCQY PGLQWVNGEK KLFYIPWRHA
60 70 80 90 100
TRHGPSQDGD NTIFKAWAKE TGKYTEGVDE ADPAKWKANL RCALNKSRDF
110 120 130 140 150
QLFYDGPRDM PPQPYKIYEV CSNGPAPTES QPTDDYVLGE EEEEEEEELQ
160 170 180 190 200
RMLPGLSITE PALPGPPNAP YSLPKEDTKW PPALQPPVGL GPPVPDPNLL
210 220 230 240 250
APPSGNPAGF RQLLPEVLEP GPLASSQPPT EPLLPDLLIS PHMLPLTDLE
260 270 280 290 300
IKFQYRGRAP RTLTISNPQG CRLFYSQLEA TQEQVELFGP VTLEQVRFPS
310 320 330 340 350
PEDIPSDKQR FYTNQLLDVL DRGLILQLQG QDLYAIRLCQ CKVFWSGPCA
360 370 380 390 400
LAHGSCPNPI QREVKTKLFS LEQFLNELIL FQKGQTNTPP PFEIFFCFGE
410 420 430 440 450
EWPDVKPREK KLITVQVVPV AARLLLEMFS GELSWSADSI RLQISNPDLK
460 470 480 490
DHMVEQFKEL HHLWQSQQQL QPMVQAPPVA GLDASQGPWP MHPVGMQ
Length:497
Mass (Da):56,005
Last modified:July 15, 1998 - v1
Checksum:iD8BD54DB946E354F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF028725 mRNA. Translation: AAB81997.1.
CCDSiCCDS19962.1.
RefSeqiNP_001239311.1. NM_001252382.1.
NP_036187.1. NM_012057.4.
XP_006505160.1. XM_006505097.1.
UniGeneiMm.6479.

Genome annotation databases

EnsembliENSMUST00000004392; ENSMUSP00000004392; ENSMUSG00000029771.
ENSMUST00000163511; ENSMUSP00000127021; ENSMUSG00000029771.
GeneIDi27056.
KEGGimmu:27056.
UCSCiuc009bdu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF028725 mRNA. Translation: AAB81997.1.
CCDSiCCDS19962.1.
RefSeqiNP_001239311.1. NM_001252382.1.
NP_036187.1. NM_012057.4.
XP_006505160.1. XM_006505097.1.
UniGeneiMm.6479.

3D structure databases

ProteinModelPortaliP56477.
SMRiP56477. Positions 19-119, 231-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205111. 4 interactions.
DIPiDIP-49382N.
IntActiP56477. 2 interactions.
MINTiMINT-1565953.
STRINGi10090.ENSMUSP00000004392.

PTM databases

iPTMnetiP56477.
PhosphoSiteiP56477.

Proteomic databases

PaxDbiP56477.
PRIDEiP56477.

Protocols and materials databases

DNASUi27056.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004392; ENSMUSP00000004392; ENSMUSG00000029771.
ENSMUST00000163511; ENSMUSP00000127021; ENSMUSG00000029771.
GeneIDi27056.
KEGGimmu:27056.
UCSCiuc009bdu.2. mouse.

Organism-specific databases

CTDi3663.
MGIiMGI:1350924. Irf5.

Phylogenomic databases

eggNOGiENOG410IFCV. Eukaryota.
ENOG410XRXT. LUCA.
HOGENOMiHOG000037433.
HOVERGENiHBG105715.
InParanoidiP56477.
KOiK09446.
OMAiYDGPRDM.
OrthoDBiEOG7CCBR1.
PhylomeDBiP56477.
TreeFamiTF328512.

Miscellaneous databases

PROiP56477.
SOURCEiSearch...

Gene expression databases

BgeeiP56477.
CleanExiMM_IRF5.
ExpressionAtlasiP56477. baseline and differential.
GenevisibleiP56477. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR029838. IRF5.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11949:SF10. PTHR11949:SF10. 1 hit.
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
SM01243. IRF-3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Generation of mutant mice deficient in IRF5."
    Grossman A., Kondo S., Antonio L., Mak T.W.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Lymph node.
  2. "Functional regulation of MyD88-activated interferon regulatory factor 5 by K63-linked polyubiquitination."
    Balkhi M.Y., Fitzgerald K.A., Pitha P.M.
    Mol. Cell. Biol. 28:7296-7308(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION AT LYS-410 AND LYS-411 BY TRAF6, SUBCELLULAR LOCATION.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiIRF5_MOUSE
AccessioniPrimary (citable) accession number: P56477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.