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P56473 (AGRP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Agouti-related protein
Gene names
Name:Agrp
Synonyms:Agrt, Art
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length131 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity By similarity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner By similarity. Ref.4

Subunit structure

Interacts with melanocortin receptors MC3R, MC4R and MC5R. Ref.4

Subcellular location

Secreted. Golgi apparatus lumen By similarity.

Tissue specificity

Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Induction

Hypothalamic expression is elevated circa 10-fold in ob/ob and db/db mice.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.

Sequence similarities

Contains 1 agouti domain.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Secreted
   DomainKnottin
Signal
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadult feeding behavior

Inferred from direct assay PubMed 17873059. Source: MGI

eating behavior

Inferred from electronic annotation. Source: Ensembl

hormone-mediated signaling pathway

Inferred from electronic annotation. Source: InterPro

maternal process involved in female pregnancy

Inferred from electronic annotation. Source: Ensembl

neuropeptide signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

photoperiodism

Inferred from electronic annotation. Source: Ensembl

positive regulation of feeding behavior

Inferred from electronic annotation. Source: Ensembl

regulation of feeding behavior

Inferred from sequence or structural similarity. Source: UniProtKB

response to insulin

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi lumen

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular space

Inferred from sequence or structural similarity. Source: UniProtKB

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionneuropeptide hormone activity

Inferred from sequence or structural similarity. Source: UniProtKB

type 3 melanocortin receptor binding

Inferred from sequence or structural similarity. Source: UniProtKB

type 4 melanocortin receptor binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 131111Agouti-related protein
PRO_0000001035

Regions

Domain86 – 12843Agouti
Region110 – 1123Interaction with melanocortin receptors By similarity

Amino acid modifications

Disulfide bond86 ↔ 101 By similarity
Disulfide bond93 ↔ 107 By similarity
Disulfide bond100 ↔ 118 By similarity
Disulfide bond104 ↔ 128 By similarity
Disulfide bond109 ↔ 116 By similarity

Sequences

Sequence LengthMass (Da)Tools
P56473 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 25D9766D074C6834

FASTA13114,432
        10         20         30         40         50         60 
MLTAMLLSCV LLLALPPTLG VQMGVAPLKG IRRPDQALFP EFPGLSLNGL KKTTADRAEE 

        70         80         90        100        110        120 
VLLQKAEALA EVLDPQNRES RSPRRCVRLH ESCLGQQVPC CDPCATCYCR FFNAFCYCRK 

       130 
LGTATNLCSR T 

« Hide

References

« Hide 'large scale' references
[1]"Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein."
Ollmann M.M., Wilson B.D., Yang Y.K., Kerns J.A., Chen Y., Gantz I., Barsh G.S.
Science 278:135-138(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 129.
[2]"Hypothalamic expression of ART, a novel gene related to agouti, is up-regulated in obese and diabetic mutant mice."
Shutter J.R., Graham M., Kinsey A.C., Scully S., Luethy R., Stark K.L.
Genes Dev. 11:593-602(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye.
[4]"Characterization of Agouti-related protein binding to melanocortin receptors."
Yang Y.K., Thompson D.A., Dickinson C.J., Wilken J., Barsh G.S., Kent S.B., Gantz I.
Mol. Endocrinol. 13:148-155(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MELANOCORTIN RECEPTORS MC3R; MC4R AND MC5R.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U89484 mRNA. Translation: AAB68620.1.
U89486 Genomic DNA. Translation: AAB68622.1.
BC079902 mRNA. Translation: AAH79902.1.
CCDSCCDS22605.1.
RefSeqNP_001258735.1. NM_001271806.1.
NP_031453.1. NM_007427.3.
UniGeneMm.441696.
Mm.491630.

3D structure databases

ProteinModelPortalP56473.
SMRP56473. Positions 86-119.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000005849.

Proteomic databases

PRIDEP56473.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005849; ENSMUSP00000005849; ENSMUSG00000005705.
GeneID11604.
KEGGmmu:11604.
UCSCuc009ndh.1. mouse.

Organism-specific databases

CTD181.
MGIMGI:892013. Agrp.

Phylogenomic databases

eggNOGNOG44906.
HOGENOMHOG000033861.
HOVERGENHBG050467.
InParanoidP56473.
KOK05231.
OMADPEGREP.
OrthoDBEOG74TX1T.
PhylomeDBP56473.
TreeFamTF330729.

Gene expression databases

ArrayExpressP56473.
BgeeP56473.
CleanExMM_AGRP.
GenevestigatorP56473.

Family and domain databases

Gene3D4.10.760.10. 1 hit.
InterProIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERPTHR16551. PTHR16551. 1 hit.
PfamPF05039. Agouti. 1 hit.
[Graphical view]
SMARTSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMSSF57055. SSF57055. 1 hit.
PROSITEPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279132.
PROP56473.
SOURCESearch...

Entry information

Entry nameAGRP_MOUSE
AccessionPrimary (citable) accession number: P56473
Secondary accession number(s): O35967
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot