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Protein

Agouti-related protein

Gene

Agrp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity (By similarity). It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner (By similarity).By similarity

GO - Molecular functioni

  1. neuropeptide hormone activity Source: UniProtKB
  2. type 3 melanocortin receptor binding Source: UniProtKB
  3. type 4 melanocortin receptor binding Source: UniProtKB

GO - Biological processi

  1. adult feeding behavior Source: MGI
  2. hormone-mediated signaling pathway Source: InterPro
  3. neuropeptide signaling pathway Source: UniProtKB
  4. regulation of feeding behavior Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Agouti-related protein
Gene namesi
Name:Agrp
Synonyms:Agrt, Art
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:892013. Agrp.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
  2. extracellular vesicular exosome Source: MGI
  3. Golgi lumen Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 131111Agouti-related proteinPRO_0000001035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi86 ↔ 101PROSITE-ProRule annotation
Disulfide bondi93 ↔ 107PROSITE-ProRule annotation
Disulfide bondi100 ↔ 118PROSITE-ProRule annotation
Disulfide bondi104 ↔ 128PROSITE-ProRule annotation
Disulfide bondi109 ↔ 116PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP56473.

Expressioni

Tissue specificityi

Expressed in arcuate nucleus and median eminence, adrenal gland (medulla), hypothalamus, testis, and lung.

Inductioni

Hypothalamic expression is elevated circa 10-fold in ob/ob and db/db mice.

Gene expression databases

BgeeiP56473.
CleanExiMM_AGRP.
ExpressionAtlasiP56473. baseline.
GenevestigatoriP56473.

Interactioni

Subunit structurei

Interacts with melanocortin receptors MC3R, MC4R and MC5R.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005849.

Structurei

3D structure databases

ProteinModelPortaliP56473.
SMRiP56473. Positions 86-119.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 12843AgoutiPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 1123Interaction with melanocortin receptorsBy similarity

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Contains 1 agouti domain.PROSITE-ProRule annotation

Keywords - Domaini

Knottin, Signal

Phylogenomic databases

eggNOGiNOG44906.
HOGENOMiHOG000033861.
HOVERGENiHBG050467.
InParanoidiP56473.
KOiK05231.
OMAiMQGAQMG.
OrthoDBiEOG74TX1T.
PhylomeDBiP56473.
TreeFamiTF330729.

Family and domain databases

Gene3Di4.10.760.10. 1 hit.
InterProiIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERiPTHR16551. PTHR16551. 1 hit.
PfamiPF05039. Agouti. 1 hit.
[Graphical view]
SMARTiSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMiSSF57055. SSF57055. 1 hit.
PROSITEiPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTAMLLSCV LLLALPPTLG VQMGVAPLKG IRRPDQALFP EFPGLSLNGL
60 70 80 90 100
KKTTADRAEE VLLQKAEALA EVLDPQNRES RSPRRCVRLH ESCLGQQVPC
110 120 130
CDPCATCYCR FFNAFCYCRK LGTATNLCSR T
Length:131
Mass (Da):14,432
Last modified:July 15, 1998 - v1
Checksum:i25D9766D074C6834
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89484 mRNA. Translation: AAB68620.1.
U89486 Genomic DNA. Translation: AAB68622.1.
BC079902 mRNA. Translation: AAH79902.1.
CCDSiCCDS22605.1.
RefSeqiNP_001258735.1. NM_001271806.1.
NP_031453.1. NM_007427.3.
UniGeneiMm.441696.
Mm.491630.

Genome annotation databases

EnsembliENSMUST00000005849; ENSMUSP00000005849; ENSMUSG00000005705.
ENSMUST00000194091; ENSMUSP00000142044; ENSMUSG00000005705.
GeneIDi11604.
KEGGimmu:11604.
UCSCiuc009ndh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89484 mRNA. Translation: AAB68620.1.
U89486 Genomic DNA. Translation: AAB68622.1.
BC079902 mRNA. Translation: AAH79902.1.
CCDSiCCDS22605.1.
RefSeqiNP_001258735.1. NM_001271806.1.
NP_031453.1. NM_007427.3.
UniGeneiMm.441696.
Mm.491630.

3D structure databases

ProteinModelPortaliP56473.
SMRiP56473. Positions 86-119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005849.

Proteomic databases

PRIDEiP56473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005849; ENSMUSP00000005849; ENSMUSG00000005705.
ENSMUST00000194091; ENSMUSP00000142044; ENSMUSG00000005705.
GeneIDi11604.
KEGGimmu:11604.
UCSCiuc009ndh.1. mouse.

Organism-specific databases

CTDi181.
MGIiMGI:892013. Agrp.

Phylogenomic databases

eggNOGiNOG44906.
HOGENOMiHOG000033861.
HOVERGENiHBG050467.
InParanoidiP56473.
KOiK05231.
OMAiMQGAQMG.
OrthoDBiEOG74TX1T.
PhylomeDBiP56473.
TreeFamiTF330729.

Miscellaneous databases

NextBioi279132.
PROiP56473.
SOURCEiSearch...

Gene expression databases

BgeeiP56473.
CleanExiMM_AGRP.
ExpressionAtlasiP56473. baseline.
GenevestigatoriP56473.

Family and domain databases

Gene3Di4.10.760.10. 1 hit.
InterProiIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERiPTHR16551. PTHR16551. 1 hit.
PfamiPF05039. Agouti. 1 hit.
[Graphical view]
SMARTiSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMiSSF57055. SSF57055. 1 hit.
PROSITEiPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Antagonism of central melanocortin receptors in vitro and in vivo by agouti-related protein."
    Ollmann M.M., Wilson B.D., Yang Y.K., Kerns J.A., Chen Y., Gantz I., Barsh G.S.
    Science 278:135-138(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129.
  2. "Hypothalamic expression of ART, a novel gene related to agouti, is up-regulated in obese and diabetic mutant mice."
    Shutter J.R., Graham M., Kinsey A.C., Scully S., Luethy R., Stark K.L.
    Genes Dev. 11:593-602(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  4. "Characterization of Agouti-related protein binding to melanocortin receptors."
    Yang Y.K., Thompson D.A., Dickinson C.J., Wilken J., Barsh G.S., Kent S.B., Gantz I.
    Mol. Endocrinol. 13:148-155(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MELANOCORTIN RECEPTORS MC3R; MC4R AND MC5R.

Entry informationi

Entry nameiAGRP_MOUSE
AccessioniPrimary (citable) accession number: P56473
Secondary accession number(s): O35967
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: March 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.