P56470 (LEG4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galectin-4
Short name=Gal-4

Alternative name(s):
Antigen NY-CO-27
L-36 lactose-binding protein
Short name=L36LBP
Lactose-binding lectin 4

Gene names

Name:

LGALS4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Subunit structure	Monomer By similarity.
Domain	Contains two homologous but distinct carbohydrate-binding domains.
Sequence similarities	Contains 2 galectin domains.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Lectin
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Traceable author statement Ref.3. Source: ProtInc
Cellular_component	cytosol Traceable author statement Ref.3. Source: ProtInc plasma membrane Traceable author statement Ref.3. Source: ProtInc
Molecular_function	carbohydrate binding Traceable author statement Ref.3. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 323

323

Galectin-4

PRO_0000076934

Regions

Domain

19 – 150

132

Galectin 1

Domain

194 – 323

130

Galectin 2

Region

256 – 262

7

Beta-galactoside binding By similarity

Natural variations

Natural variant

16

1

T → M.
Corresponds to variant rs8106404 [ dbSNP | Ensembl ].

VAR_049769

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

323

Helix Strand Turn

Details...

Beta strand

187 – 189

3

Beta strand

192 – 197

6

Beta strand

206 – 213

8

Beta strand

221 – 226

6

Beta strand

228 – 230

3

Beta strand

234 – 238

5

Turn

241 – 243

3

Beta strand

247 – 249

3

Beta strand

272 – 280

9

Beta strand

282 – 298

17

Helix

302 – 306

5

Beta strand

309 – 315

7

Beta strand

317 – 323

7

Sequences

	Sequence		Length	Mass (Da)
	P56470 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: E79EC0A9AB3990EF	FASTA	323	35,941
	10 20 30 40 50 60 MAYVPAPGYQ PTYNPTLPYY QPIPGGLNVG MSVYIQGVAS EHMKRFFVNF VVGQDPGSDV 70 80 90 100 110 120 AFHFNPRFDG WDKVVFNTLQ GGKWGSEERK RSMPFKKGAA FELVFIVLAE HYKVVVNGNP 130 140 150 160 170 180 FYEYGHRLPL QMVTHLQVDG DLQLQSINFI GGQPLRPQGP PMMPPYPGPG HCHQQLNSLP 190 200 210 220 230 240 TMEGPPTFNP PVPYFGRLQG GLTARRTIII KGYVPPTGKS FAINFKVGSS GDIALHINPR 250 260 270 280 290 300 MGNGTVVRNS LLNGSWGSEE KKITHNPFGP GQFFDLSIRC GLDRFKVYAN GQHLFDFAHR 310 320 LSAFQRVDTL EIQGDVTLSY VQI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kato S. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gastric carcinoma.
[2]	"Cloning and expression of the mRNA of human galectin-4, an S-type lectin down-regulated in colorectal cancer." Rechreche H., Mallo G.V., Montalto G., Dagorn J.-C., Iovanna J.L. Eur. J. Biochem. 248:225-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon carcinoma.
[3]	"Strikingly different localization of galectin-3 and galectin-4 in human colon adenocarcinoma T84 cells. Galectin-4 is localized at sites of cell adhesion." Huflejt M.E., Jordan E.T., Gitt M.A., Barondes S.H., Leffler H. J. Biol. Chem. 272:14294-14303(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon and Skin.
[5]	"Solution structure of the C-terminal Gal-bind lectin domain from human galectin-4." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 173-323.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Galectin-4

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB006781 mRNA. Translation: BAA22165.1.
AF014838 mRNA. Translation: AAC51763.1.
U82953 mRNA. Translation: AAB86590.1.
BC003661 mRNA. Translation: AAH03661.1.
BC005146 mRNA. Translation: AAH05146.1.
BC034750 mRNA. Translation: AAH34750.1.

IPI

IPI00009750.

RefSeq

NP_006140.1. NM_006149.3.

UniGene

Hs.5302.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X50	NMR	-	A	173-323	[»]

ProteinModelPortal

P56470.

ModBase

Search...

Protein-protein interaction databases

IntAct

P56470. 5 interactions.

MINT

MINT-1401408.

STRING

9606.ENSP00000302100.

PTM databases

PhosphoSite

P56470.

Polymorphism databases

DMDM

3024079.

Proteomic databases

PaxDb

P56470.

PRIDE

P56470.

Protocols and materials databases

DNASU

3960.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000307751; ENSP00000302100; ENSG00000171747.

GeneID

3960.

KEGG

hsa:3960.

UCSC

uc002ojg.3. human.

Organism-specific databases

CTD

3960.

GeneCards

GC19M039292.

HGNC

HGNC:6565. LGALS4.

HPA

CAB025653.

MIM

602518. gene.

neXtProt

NX_P56470.

PharmGKB

PA30342.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG264430.

HOGENOM

HOG000290194.

HOVERGEN

HBG002412.

InParanoid

P56470.

KO

K10091.

OMA

GQYFDLS.

OrthoDB

EOG4WH8MT.

PhylomeDB

P56470.

Gene expression databases

ArrayExpress

P56470.

Bgee

P56470.

CleanEx

HS_LGALS4.

Genevestigator

P56470.

GermOnline

ENSG00000171747. Homo sapiens.

Family and domain databases

Gene3D

2.60.120.200. 2 hits.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR015533. Galectin4/6.
IPR001079. Galectin_CRD.
[Graphical view]

PANTHER

PTHR11346:SF23. PTHR11346:SF23. 1 hit.

Pfam

PF00337. Gal-bind_lectin. 2 hits.
[Graphical view]

SMART

SM00908. Gal-bind_lectin. 2 hits.
SM00276. GLECT. 2 hits.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 2 hits.

PROSITE

PS51304. GALECTIN. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P56470.

ChEMBL

CHEMBL1671608.

ChiTaRS

LGALS4. human.

EvolutionaryTrace

P56470.

GenomeRNAi

3960.

NextBio

15540.

SOURCE

Search...

Entry information

Entry name

LEG4_HUMAN

Accession

Primary (citable) accession number: P56470

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.