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Protein

Adenylosuccinate lyase

Gene

purB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP.
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathway: AMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes AMP from IMP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase (purA)
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei141 – 1411Proton donor/acceptorBy similarity
Binding sitei212 – 2121SubstrateBy similarity
Active sitei262 – 2621Proton donor/acceptorBy similarity
Binding sitei276 – 2761SubstrateBy similarity
Binding sitei301 – 3011SubstrateBy similarity
Binding sitei306 – 3061SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciHPY:HP1112-MONOMER.
UniPathwayiUPA00074; UER00132.
UPA00075; UER00336.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate lyase (EC:4.3.2.2)
Short name:
ASL
Alternative name(s):
Adenylosuccinase
Short name:
ASase
Gene namesi
Name:purB
Ordered Locus Names:HP_1112
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Adenylosuccinate lyasePRO_0000137881Add
BLAST

Proteomic databases

PRIDEiP56468.

Interactioni

Subunit structurei

Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site (By similarity).By similarity

Protein-protein interaction databases

STRINGi85962.HP1112.

Structurei

3D structure databases

ProteinModelPortaliP56468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 52Substrate bindingBy similarity
Regioni67 – 693Substrate bindingBy similarity
Regioni93 – 942Substrate bindingBy similarity
Regioni268 – 2703Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0015.
KOiK01756.
OMAiPKNMLKN.
OrthoDBiEOG686NDB.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR004769. Pur_lyase.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00928. purB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56468-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLERYANEEM KALWNEQTKF ETYLEVEKAV VRAWNKLGQI QDSDCEKICA
60 70 80 90 100
KAAFNLERIK EIEKTTKHDL IAFTTCVAES LGEESRFFHY GITSSDCIDT
110 120 130 140 150
AMALLMTKSL KLIQKGVKNL YETLKNRALE HKDTLMVGRS HGVFGEPITF
160 170 180 190 200
GLVLALFADE IKRHLKALDL TMEFISVGAI SGAMGNFAHA PLELEELACE
210 220 230 240 250
FLGLKTANIS NQVIQRDRYA RLACDLALLA SSCEKIAVNI RHLQRSEVYE
260 270 280 290 300
VEEAFSTGQK GSSAMPHKRN PILSENITGL CRVIRSFTTP MLENVALWHE
310 320 330 340 350
RDMSHSSVER FALPDLFITS DFMLSRLNSV IKNLVVYPKN MLKNLALSGG
360 370 380 390 400
LVFSQRVLLE LPKKGLSREE SYSIVQENAM KIWEVLQQGA FKNTDENLFL
410 420 430 440
NALLNDERLK KYLSEDEIKA CFDYNYYTKN VGAIFKRVFE
Length:440
Mass (Da):50,029
Last modified:July 15, 1998 - v1
Checksum:i59587A4517404A46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08156.1.
PIRiH64658.
RefSeqiNP_207903.1. NC_000915.1.
WP_000893828.1. NC_018939.1.
YP_006935027.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08156; AAD08156; HP_1112.
GeneIDi899648.
KEGGiheo:C694_05735.
hpy:HP1112.
PATRICi20593565. VBIHelPyl33062_1162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08156.1.
PIRiH64658.
RefSeqiNP_207903.1. NC_000915.1.
WP_000893828.1. NC_018939.1.
YP_006935027.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP56468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP1112.

Proteomic databases

PRIDEiP56468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08156; AAD08156; HP_1112.
GeneIDi899648.
KEGGiheo:C694_05735.
hpy:HP1112.
PATRICi20593565. VBIHelPyl33062_1162.

Phylogenomic databases

eggNOGiCOG0015.
KOiK01756.
OMAiPKNMLKN.
OrthoDBiEOG686NDB.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00132.
UPA00075; UER00336.
BioCyciHPY:HP1112-MONOMER.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR004769. Pur_lyase.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00928. purB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiPUR8_HELPY
AccessioniPrimary (citable) accession number: P56468
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: May 27, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.