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P56468 (PUR8_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate lyase

Short name=ASL
EC=4.3.2.2
Alternative name(s):
Adenylosuccinase
Short name=ASase
Gene names
Name:purB
Ordered Locus Names:HP_1112
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP.

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.

Subunit structure

Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site By similarity.

Sequence similarities

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Adenylosuccinate lyase
PRO_0000137881

Regions

Region4 – 52Substrate binding By similarity
Region67 – 693Substrate binding By similarity
Region93 – 942Substrate binding By similarity
Region268 – 2703Substrate binding By similarity

Sites

Active site1411Proton donor/acceptor By similarity
Active site2621Proton donor/acceptor By similarity
Binding site2121Substrate By similarity
Binding site2761Substrate By similarity
Binding site3011Substrate By similarity
Binding site3061Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P56468 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 59587A4517404A46

FASTA44050,029
        10         20         30         40         50         60 
MLERYANEEM KALWNEQTKF ETYLEVEKAV VRAWNKLGQI QDSDCEKICA KAAFNLERIK 

        70         80         90        100        110        120 
EIEKTTKHDL IAFTTCVAES LGEESRFFHY GITSSDCIDT AMALLMTKSL KLIQKGVKNL 

       130        140        150        160        170        180 
YETLKNRALE HKDTLMVGRS HGVFGEPITF GLVLALFADE IKRHLKALDL TMEFISVGAI 

       190        200        210        220        230        240 
SGAMGNFAHA PLELEELACE FLGLKTANIS NQVIQRDRYA RLACDLALLA SSCEKIAVNI 

       250        260        270        280        290        300 
RHLQRSEVYE VEEAFSTGQK GSSAMPHKRN PILSENITGL CRVIRSFTTP MLENVALWHE 

       310        320        330        340        350        360 
RDMSHSSVER FALPDLFITS DFMLSRLNSV IKNLVVYPKN MLKNLALSGG LVFSQRVLLE 

       370        380        390        400        410        420 
LPKKGLSREE SYSIVQENAM KIWEVLQQGA FKNTDENLFL NALLNDERLK KYLSEDEIKA 

       430        440 
CFDYNYYTKN VGAIFKRVFE 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08156.1.
PIRH64658.
RefSeqNP_207903.1. NC_000915.1.
YP_006935027.1. NC_018939.1.

3D structure databases

ProteinModelPortalP56468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85962.HP1112.

Proteomic databases

PRIDEP56468.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08156; AAD08156; HP_1112.
GeneID13870305.
899648.
KEGGheo:C694_05735.
hpy:HP1112.
PATRIC20593565. VBIHelPyl33062_1162.

Phylogenomic databases

eggNOGCOG0015.
KOK01756.
OMAQRSEVYE.
OrthoDBEOG686NDB.
ProtClustDBPRK08470.

Enzyme and pathway databases

BioCycHPY:HP1112-MONOMER.
UniPathwayUPA00074; UER00132.
UPA00075; UER00336.

Family and domain databases

Gene3D1.10.275.10. 1 hit.
InterProIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR004769. Pur_lyase.
[Graphical view]
PANTHERPTHR11444. PTHR11444. 1 hit.
PTHR11444:SF2. PTHR11444:SF2. 1 hit.
PfamPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
SMARTSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMSSF48557. SSF48557. 1 hit.
TIGRFAMsTIGR00928. purB. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR8_HELPY
AccessionPrimary (citable) accession number: P56468
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: December 11, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names