Reviewed, UniProtKB/Swiss-Prot P56465 (PYRC_HELPY)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	HP_0581

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Dihydroorotase		PRO_0000147208
Sites
	Metal binding	12	1	Zinc 1 By similarity
	Metal binding	14	1	Zinc 1 By similarity
	Metal binding	94	1	Zinc 1; via carbamate group By similarity
	Metal binding	94	1	Zinc 2; via carbamate group By similarity
	Metal binding	133	1	Zinc 2 By similarity
	Metal binding	167	1	Zinc 2 By similarity
	Metal binding	239	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	94	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56465-1 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: DC0186A914FA11E4	FASTA	339	37,883
	10 20 30 40 50 60 MEITLFDPID AHLHVRENAL LKAVLGYSSE PFSAAVIMPN LSKPLIDTPT TLEYEEEILN 70 80 90 100 110 120 HSSNFKPLMS LYFNDGLTLE ELQCAKEKGV RFLKLYPKGM TTNAQNGTSD LLGEKTLEVL 130 140 150 160 170 180 ENAQKLGFIL CIHAEQTGFC LDKEFLCHSV LETFALSFPK LKIIIEHLSD WRSIALIEKH 190 200 210 220 230 240 DNLYATLTLH HISMTLDDLL GGSLNPHCFC KPLIKTKKDQ ERLLSLALKA HPKISFGSDS 250 260 270 280 290 300 APHFISKKHS ANIPAGIFSA PILLPALCEL FEKHNALENL QAFISDNAKT IYGLENLPSK 310 320 330 KARLSKKPFM IPTHTLCLNE KIAILRGGET LSWNLQEIA

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07635.1.
PIR	E64592.
RefSeq	NP_207376.1.
3D structure databases
HSSP	HSSP built from PDB template 1J79 based on UniProtKB P05020.
ModBase	Search...
Genome annotation databases
GeneID	900032.
GenomeReviews	Gene locus HP_0581 in contig AE000511_GR.
KEGG	hpy:HP0581.
NMPDR	fig|85962.1.peg.574.
TIGR	HP_0581.
Phylogenomic databases
HOGENOM	P56465.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006992. Amidohydro_2. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF04909. Amidohydro_2. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_HELPY

Accession

Primary (citable) accession number: P56465

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	November 25, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)