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P56456

- SYI_HELPY

UniProt

P56456 - SYI_HELPY

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHPY:HP1422-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HP_1422
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 920920Isoleucine--tRNA ligasePRO_0000098398Add
BLAST

Proteomic databases

PRIDEiP56456.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3571N.
IntActiP56456. 3 interactions.
MINTiMINT-187219.
STRINGi85962.HP1422.

Structurei

3D structure databases

ProteinModelPortaliP56456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56456-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEYKDTLNL NTTTFSMKGN LSVNEPKTYA KWQEQQAFKR MQARKDNHGD
60 70 80 90 100
FTLHDGPPYA NGHLHLGHAL NKILKDIVVK REYFKGKKIY YTPGWDCHGL
110 120 130 140 150
PIEQQILERL EKEKTSLENP TLFREKCRDH AKKFLEIQKN EFLQLGVLGD
160 170 180 190 200
FEDPYKTMDF KFEASIYRAL VEVAKKGLLK ERHKPIYWSY ACESALAEAE
210 220 230 240 250
VEYKMKKSPS IFVAFGLKKE SLEKLKVKKA SLVIWTTTPW TLYANVAIAL
260 270 280 290 300
KKDAVYALTQ KGYLVAKALH EKLAALGVVD NEITHEFNSN DLEYLVATNP
310 320 330 340 350
LNQRDSLVAL GEHVGLEDGT GAVHTAPGHG EEDYYLGLRY NLEVLMSVDE
360 370 380 390 400
KGCYDEGIIH NQLLDESYLG EHVFKAQKRI IEQLGDSLLL EQEIEHSYPH
410 420 430 440 450
CWRTHKPVIY RATTQWFILM DEPFIQNDGS QKTLREVALD AIEKVEFVPS
460 470 480 490 500
SGKNRLKTMI ENRPDWCLSR QRKWGVPLAF FIDKRTNKPC FESEVLEHVA
510 520 530 540 550
NLFEKKGCDV WWEYSVKDLL PPSYQEDAKH YEKIMHILDV WFDSGSTFKA
560 570 580 590 600
VLEDYHGEKG QSPSDVILEG SDQHRGWFQS SLLIGCVLNN QAPFKKVITH
610 620 630 640 650
GFIVDEKGEK MSKSKGNVVS LDKLLKTHGS DVVRLWVAFN DYQNDLRVSQ
660 670 680 690 700
TFFTQTEQHY KKFRNTLKFL LANFSDMDLK NLERPHNFSP LDHFMLETLE
710 720 730 740 750
TISAGVNSAF EEHDFVKGLN ILMAFVTNEL SGIYLDACKD SLYCDSKNNE
760 770 780 790 800
KRQAIQMVLL ATASKLCYFL APILTHTIEE VLEHSQALRI FLQAKDVFDL
810 820 830 840 850
KDISVSEKLH LKEFKKPENF EAVLALRSAF NEELDRLKKE GVIKNSLECA
860 870 880 890 900
IEVKEKALDE NLVEELLMVS FVGIAKEKLS ETPAFTLFKA PFYKCPRCWR
910 920
FKSELENTPC KRCEQVLKER
Length:920
Mass (Da):106,079
Last modified:July 15, 1998 - v1
Checksum:iE6AFE8C21D84C69A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08464.1.
PIRiF64697.
RefSeqiNP_208213.1. NC_000915.1.
WP_000393434.1. NC_000915.1.

Genome annotation databases

EnsemblBacteriaiAAD08464; AAD08464; HP_1422.
GeneIDi899873.
KEGGihpy:HP1422.
PATRICi20594243. VBIHelPyl33062_1491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08464.1 .
PIRi F64697.
RefSeqi NP_208213.1. NC_000915.1.
WP_000393434.1. NC_000915.1.

3D structure databases

ProteinModelPortali P56456.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-3571N.
IntActi P56456. 3 interactions.
MINTi MINT-187219.
STRINGi 85962.HP1422.

Proteomic databases

PRIDEi P56456.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD08464 ; AAD08464 ; HP_1422 .
GeneIDi 899873.
KEGGi hpy:HP1422.
PATRICi 20594243. VBIHelPyl33062_1491.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HPY:HP1422-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiSYI_HELPY
AccessioniPrimary (citable) accession number: P56456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3