P56453 (SYGA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	HP_0960

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Glycine--tRNA ligase alpha subunit HAMAP-Rule MF_00254		PRO_0000072842

Sequences

	Sequence		Length	Mass (Da)
	P56453 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 08B5F386AED651D4	FASTA	303	34,936
	10 20 30 40 50 60 MQDFSSLLLK LQEYWKNQGC LVIQPYDIPA GAGTFHPATL LRSLDKKPWN VAYVAPSRRP 70 80 90 100 110 120 TDGRYGENPN RLGSYYQFQV VIKPSPSNIQ ELYLKSLEVL GINLNEHDIR FVEDNWESPT 130 140 150 160 170 180 LGAWGLGWEV WLDGMEVTQF TYFQQVGGIA CSPIPVEITY GLERLAMYVQ KVENILEIEW 190 200 210 220 230 240 AKKNHDSVNY AQVHLESEYE FSKYHFETAS VKRLLEMFKN AQAEALHCLE NKLPLPAYDF 250 260 270 280 290 300 VMLCSHFFNI LDARKAISVA ERQNYILQIR DLAKGCALLY KEQEEEREER LKNALTKAEN GVS

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08002.1.
PIR	H64639.
RefSeq	NP_207752.1. NC_000915.1. YP_006934877.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P56453.
SMR	P56453. Positions 4-282.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP0960.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD08002; AAD08002; HP_0960.
GeneID	13870153. 899493.
KEGG	heo:C694_04945. hpy:HP0960.
PATRIC	20593233. VBIHelPyl33062_0998.
Phylogenomic databases
eggNOG	COG0752.
KO	K01878.
OMA	CVILQPY.
ProtClustDB	PRK09348.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha.
InterPro	IPR006194. Gly-tRNA-synth_heterodimer. IPR002310. Gly-tRNA_ligase_asu. [Graphical view]
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. glyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_HELPY

Accession

Primary (citable) accession number: P56453

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program