P56435 (CMA1_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymase
EC=3.4.21.39

Alternative name(s):
Alpha-chymase

Gene names

Name:

CMA1

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion By similarity.
Catalytic activity	Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.
Subcellular location	Secreted By similarity. Cytoplasmic granule By similarity. Note: Mast cell granules By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Propeptide	20 – 21	2	Activation peptide		PRO_0000027435
	Chain	22 – 247	226	Chymase		PRO_0000027436
Regions
	Domain	22 – 245	224	Peptidase S1
Sites
	Active site	66	1	Charge relay system By similarity
	Active site	110	1	Charge relay system By similarity
	Active site	203	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	144 ↔ 209		By similarity
	Disulfide bond	175 ↔ 188		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56435 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 6D049B34377FC8B9	FASTA	247	27,400
	10 20 30 40 50 60 MLLLPLPLLL FFLCSRAEAG EIIGGTECKP HSRPYMAYLE IVTSNGPSKS CGGFLIRRNF 70 80 90 100 110 120 VLTAVHCAGR SITVTLGAHN ITEKEDTWQK LEVIKQFRHP KYNTSTLHHD IMLLKLKEKA 130 140 150 160 170 180 SLTLAVGTLP FPSQFNFVPP GRMCRVAGWG RTGVLKPGSD TLQEVKLRLM DPQACSHFRY 190 200 210 220 230 240 FDHNLQLCVG NPRKTKSAFK GDSGGPLLCA GVAQGIVSYG RLDAKPPAVF TRISHYRPWI NKILQAN

References

[1]	"Induction of chymase that forms angiotensin II in the monkey atherosclerotic aorta." Takai S., Shiota N., Kobayashi S., Matsumura E., Miyazaki M. FEBS Lett. 412:86-90(1997) [PubMed: 9257695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB000823 mRNA. Translation: BAA22070.1.
3D structure databases
ProteinModelPortal	P56435.
SMR	P56435. Positions 22-247.
ModBase	Search...
Protein family/group databases
MEROPS	S01.140.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG013304.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMA1_MACFA

Accession

Primary (citable) accession number: P56435

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program