Reviewed,
UniProtKB/Swiss-Prot P56435 (CMA1_MACFA)
Last modified
June 16, 2009.
Version 55.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Chymase EC=3.4.21.39 Alternative name(s): Alpha-chymase | ||
| Gene names |
| ||
| Organism | Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey) | ||
| Taxonomic identifier | 9541 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca |
Protein attributes
| Sequence length | 247 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion By similarity. |
| Catalytic activity | Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa. |
| Subcellular location | Secreted By similarity. Cytoplasmic granule By similarity. Note: Mast cell granules By similarity. |
| Sequence similarities | Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Domain | Signal |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Glycoprotein Zymogen |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | By similarity | ||||||||
| Propeptide | 20 – 21 | 2 | Activation peptide | PRO_0000027435 | |||||||
| Chain | 22 – 247 | 226 | Chymase | PRO_0000027436 | |||||||
Regions | |||||||||||
| Domain | 22 – 245 | 224 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 66 | 1 | Charge relay system By similarity | ||||||||
| Active site | 110 | 1 | Charge relay system By similarity | ||||||||
| Active site | 203 | 1 | Charge relay system By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 80 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 103 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 51 ↔ 67 | By similarity | |||||||||
| Disulfide bond | 144 ↔ 209 | By similarity | |||||||||
| Disulfide bond | 175 ↔ 188 | By similarity | |||||||||
Sequences
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References
| [1] | "Induction of chymase that forms angiotensin II in the monkey atherosclerotic aorta." Takai S., Shiota N., Kobayashi S., Matsumura E., Miyazaki M. FEBS Lett. 412:86-90(1997) [PubMed: 9257695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| AB000823 mRNA. Translation: BAA22070.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1NN6 based on UniProtKB P23946. |
| SMR | P56435. Positions 22-247. |
| ModBase | Search... |
Phylogenomic databases | |
| HOVERGEN | P56435. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.39. 3438. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CMA1_MACFA | ||||||||
| Accession | Primary (citable) accession number: P56435 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


