Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14GTPUniRule annotation1
Metal bindingi21Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi25Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei27S-adenosyl-L-methionineUniRule annotation1
Metal bindingi28Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei64GTPUniRule annotation1
Binding sitei68S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei95GTPUniRule annotation1
Binding sitei119S-adenosyl-L-methionineUniRule annotation1
Binding sitei155GTPUniRule annotation1
Binding sitei189S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi249Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi252Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi266Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi254 – 256GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHPY:HP0768-MONOMER.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:HP_0768
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001529671 – 321GTP 3',8-cyclaseAdd BLAST321

Proteomic databases

PaxDbiP56414.

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi85962.HP0768.

Structurei

3D structure databases

ProteinModelPortaliP56414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CM1. Bacteria.
COG2896. LUCA.
KOiK03639.
OMAiTKLNCVV.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56414-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVDSFNRVI DYIRVSVTKQ CNFRCQYCMP ATPLNFFDNE ELLPLDNVLE
60 70 80 90 100
FLKIAIDEGV KKIRITGGEP LLRKGLDEFI AKLHAYNKEV ELVLSTNGFL
110 120 130 140 150
LKKMAKDLKN AGLAQVNVSL DSLKSDRVLK ISQKDALKNT LEGIEESLKV
160 170 180 190 200
GLKLKLNTVV IKSVNDDEIL ELLEYAKNRH IQIRYIEFME NTHAKSLVKG
210 220 230 240 250
LKEREILDLI AQKYQIIEAE KPKQGSSKIY TLENGYQFGI IAPHSDDFCQ
260 270 280 290 300
SCNRIRLASD GKICPCLYYQ DAIDAKEAII NKDTKNIKRL LKQSVINKPE
310 320
KNMWNDKNSE TPTRAFYYTG G
Length:321
Mass (Da):36,657
Last modified:July 15, 1998 - v1
Checksum:i3308D812C8A6950C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07817.1.
PIRiH64615.
RefSeqiNP_207561.1. NC_000915.1.
WP_001863441.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07817; AAD07817; HP_0768.
GeneIDi899338.
KEGGiheo:C694_03945.
hpy:HP0768.
PATRICi20592825. VBIHelPyl33062_0801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07817.1.
PIRiH64615.
RefSeqiNP_207561.1. NC_000915.1.
WP_001863441.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP56414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0768.

Proteomic databases

PaxDbiP56414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07817; AAD07817; HP_0768.
GeneIDi899338.
KEGGiheo:C694_03945.
hpy:HP0768.
PATRICi20592825. VBIHelPyl33062_0801.

Phylogenomic databases

eggNOGiENOG4105CM1. Bacteria.
COG2896. LUCA.
KOiK03639.
OMAiTKLNCVV.

Enzyme and pathway databases

UniPathwayiUPA00344.
BioCyciHPY:HP0768-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_HELPY
AccessioniPrimary (citable) accession number: P56414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.