P56406 (SNPA_STRCS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Extracellular small neutral protease
EC=3.4.24.77

Alternative name(s):
SCNP
Snapalysin

Gene names

Name:

snpA

Organism

Streptomyces caespitosus

Taxonomic identifier

53502 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically hydrolyzes the peptide bond at the imino side of aromatic residues.
Catalytic activity	Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.
Cofactor	Binds 1 calcium ion per subunit. Binds 1 zinc ion per subunit.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase M7 family.

Ontologies

Keywords
Cellular component	Secreted
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 132

132

Extracellular small neutral protease

PRO_0000078176

Sites

Active site

84

1

Metal binding

76

1

Calcium

Metal binding

78

1

Calcium

Metal binding

83

1

Zinc; catalytic

Metal binding

87

1

Zinc; catalytic

Metal binding

93

1

Zinc; catalytic

Amino acid modifications

Disulfide bond

99 ↔ 112

Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

132

Helix Strand Turn

Details...

Beta strand

2 – 10

9

Helix

12 – 14

3

Helix

15 – 28

14

Beta strand

30 – 36

7

Beta strand

41 – 47

7

Beta strand

54 – 57

4

Beta strand

59 – 61

3

Beta strand

63 – 68

6

Helix

69 – 74

6

Helix

77 – 89

13

Helix

102 – 104

3

Turn

105 – 108

4

Helix

119 – 128

10

Turn

129 – 131

3

Sequences

	Sequence		Length	Mass (Da)
	P56406 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 7CB988AFC2F0B1E4	FASTA	132	14,376
	10 20 30 40 50 60 TVTVTYDPSN APSFQQEIAN AAQIWNSSVR NVQLRAGGNA DFSYYEGNDS RGSYAQTDGH 70 80 90 100 110 120 GRGYIFLDYQ QNQQYDSTRV TAHETGHVLG LPDHYQGPCS ELMSGGGPGP SCTNPYPNAQ 130 ERSRVNALWA NG

References

[1]	"Complete amino acid sequence of a zinc metalloendoprotease from Streptomyces caespitosus." Harada S., Kinoshita T., Kasai N., Tsunasawa S., Sakiyama F. Eur. J. Biochem. 233:683-686(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Structure of the zinc endoprotease from Streptomyces caespitosus." Kurisu G., Kinoshita T., Sugimoto A., Nagara A., Kai Y., Kasai N., Harada S. J. Biochem. 121:304-308(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S63978.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C7K	X-ray	1.00	A	1-132	[»]
1KUH	X-ray	1.60	A	1-132	[»]
4HX3	X-ray	2.70	A/C/E/G/I/K	2-132	[»]

ProteinModelPortal

P56406.

SMR

P56406. Positions 1-132.

ModBase

Search...

Protein family/group databases

MEROPS

M07.001.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.40.390.10. 1 hit.

InterPro

IPR024079. MetalloPept_cat_dom.
IPR000013. Peptidase_M7.
[Graphical view]

Pfam

PF02031. Peptidase_M7. 1 hit.
[Graphical view]

PIRSF

PIRSF016573. Peptidase_M7. 1 hit.

PRINTS

PR00787. NEUTRALPTASE.

ProDom

PD016028. Peptidase_M7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00142. ZINC_PROTEASE. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P56406.

Entry information

Entry name

SNPA_STRCS

Accession

Primary (citable) accession number: P56406

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program