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P56406 (SNPA_STRCS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Extracellular small neutral protease

EC=3.4.24.77
Alternative name(s):
SCNP
Snapalysin
Gene names
Name:snpA
OrganismStreptomyces caespitosus
Taxonomic identifier53502 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length132 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically hydrolyzes the peptide bond at the imino side of aromatic residues.

Catalytic activity

Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.

Cofactor

Binds 1 calcium ion per subunit.

Binds 1 zinc ion per subunit.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M7 family.

Ontologies

Keywords
   Cellular componentSecreted
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 132132Extracellular small neutral protease
PRO_0000078176

Sites

Active site841
Metal binding761Calcium
Metal binding781Calcium
Metal binding831Zinc; catalytic
Metal binding871Zinc; catalytic
Metal binding931Zinc; catalytic

Amino acid modifications

Disulfide bond99 ↔ 112 Ref.1

Secondary structure

......................... 132
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P56406 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: 7CB988AFC2F0B1E4

FASTA13214,376
        10         20         30         40         50         60 
TVTVTYDPSN APSFQQEIAN AAQIWNSSVR NVQLRAGGNA DFSYYEGNDS RGSYAQTDGH 

        70         80         90        100        110        120 
GRGYIFLDYQ QNQQYDSTRV TAHETGHVLG LPDHYQGPCS ELMSGGGPGP SCTNPYPNAQ 

       130 
ERSRVNALWA NG 

« Hide

References

[1]"Complete amino acid sequence of a zinc metalloendoprotease from Streptomyces caespitosus."
Harada S., Kinoshita T., Kasai N., Tsunasawa S., Sakiyama F.
Eur. J. Biochem. 233:683-686(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
[2]"Structure of the zinc endoprotease from Streptomyces caespitosus."
Kurisu G., Kinoshita T., Sugimoto A., Nagara A., Kai Y., Kasai N., Harada S.
J. Biochem. 121:304-308(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

PIRS63978.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1C7KX-ray1.00A1-132[»]
1KUHX-ray1.60A1-132[»]
4HX3X-ray2.70A/C/E/G/I/K2-132[»]
ProteinModelPortalP56406.
SMRP56406. Positions 1-132.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM07.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.390.10. 1 hit.
InterProIPR024079. MetalloPept_cat_dom.
IPR000013. Peptidase_M7.
[Graphical view]
PfamPF02031. Peptidase_M7. 1 hit.
[Graphical view]
PIRSFPIRSF016573. Peptidase_M7. 1 hit.
PRINTSPR00787. NEUTRALPTASE.
ProDomPD016028. Peptidase_M7. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

EvolutionaryTraceP56406.

Entry information

Entry nameSNPA_STRCS
AccessionPrimary (citable) accession number: P56406
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references