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P56406

- SNPA_STRCS

UniProt

P56406 - SNPA_STRCS

Protein

Extracellular small neutral protease

Gene

snpA

Organism
Streptomyces caespitosus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Specifically hydrolyzes the peptide bond at the imino side of aromatic residues.

    Catalytic activityi

    Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.

    Cofactori

    Binds 1 calcium ion per subunit.
    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi76 – 761Calcium
    Metal bindingi78 – 781Calcium
    Metal bindingi83 – 831Zinc; catalytic
    Active sitei84 – 841
    Metal bindingi87 – 871Zinc; catalytic
    Metal bindingi93 – 931Zinc; catalytic

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: InterPro
    2. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM07.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Extracellular small neutral protease (EC:3.4.24.77)
    Alternative name(s):
    SCNP
    Snapalysin
    Gene namesi
    Name:snpA
    OrganismiStreptomyces caespitosus
    Taxonomic identifieri53502 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 132132Extracellular small neutral proteasePRO_0000078176Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi99 ↔ 1121 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    132
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109
    Helixi12 – 143
    Helixi15 – 2814
    Beta strandi30 – 367
    Beta strandi41 – 477
    Beta strandi54 – 574
    Beta strandi59 – 613
    Beta strandi63 – 686
    Helixi69 – 746
    Helixi77 – 8913
    Helixi102 – 1043
    Turni105 – 1084
    Helixi119 – 12810
    Turni129 – 1313

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1C7KX-ray1.00A1-132[»]
    1KUHX-ray1.60A1-132[»]
    4HX3X-ray2.70A/C/E/G/I/K2-132[»]
    ProteinModelPortaliP56406.
    SMRiP56406. Positions 1-132.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP56406.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M7 family.Curated

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR000013. Peptidase_M7.
    [Graphical view]
    PfamiPF02031. Peptidase_M7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016573. Peptidase_M7. 1 hit.
    PRINTSiPR00787. NEUTRALPTASE.
    ProDomiPD016028. Peptidase_M7. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P56406-1 [UniParc]FASTAAdd to Basket

    « Hide

    TVTVTYDPSN APSFQQEIAN AAQIWNSSVR NVQLRAGGNA DFSYYEGNDS    50
    RGSYAQTDGH GRGYIFLDYQ QNQQYDSTRV TAHETGHVLG LPDHYQGPCS 100
    ELMSGGGPGP SCTNPYPNAQ ERSRVNALWA NG 132
    Length:132
    Mass (Da):14,376
    Last modified:July 15, 1998 - v1
    Checksum:i7CB988AFC2F0B1E4
    GO

    Sequence databases

    PIRiS63978.

    Cross-referencesi

    Sequence databases

    PIRi S63978.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1C7K X-ray 1.00 A 1-132 [» ]
    1KUH X-ray 1.60 A 1-132 [» ]
    4HX3 X-ray 2.70 A/C/E/G/I/K 2-132 [» ]
    ProteinModelPortali P56406.
    SMRi P56406. Positions 1-132.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M07.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P56406.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR000013. Peptidase_M7.
    [Graphical view ]
    Pfami PF02031. Peptidase_M7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016573. Peptidase_M7. 1 hit.
    PRINTSi PR00787. NEUTRALPTASE.
    ProDomi PD016028. Peptidase_M7. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete amino acid sequence of a zinc metalloendoprotease from Streptomyces caespitosus."
      Harada S., Kinoshita T., Kasai N., Tsunasawa S., Sakiyama F.
      Eur. J. Biochem. 233:683-686(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    2. "Structure of the zinc endoprotease from Streptomyces caespitosus."
      Kurisu G., Kinoshita T., Sugimoto A., Nagara A., Kai Y., Kasai N., Harada S.
      J. Biochem. 121:304-308(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiSNPA_STRCS
    AccessioniPrimary (citable) accession number: P56406
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3