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Protein

Extracellular small neutral protease

Gene

snpA

Organism
Streptomyces caespitosus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specifically hydrolyzes the peptide bond at the imino side of aromatic residues.

Catalytic activityi

Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi76Calcium1 Publication1
Metal bindingi78Calcium1 Publication1
Metal bindingi83Zinc; catalytic1 Publication1
Active sitei841
Metal bindingi87Zinc; catalytic1 Publication1
Metal bindingi93Zinc; catalytic1 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM07.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular small neutral protease (EC:3.4.24.77)
Alternative name(s):
SCNP
Snapalysin
Gene namesi
Name:snpA
OrganismiStreptomyces caespitosus
Taxonomic identifieri53502 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000781761 – 132Extracellular small neutral proteaseAdd BLAST132

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi99 ↔ 1122 Publications

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1132
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Helixi12 – 14Combined sources3
Helixi15 – 28Combined sources14
Beta strandi30 – 36Combined sources7
Beta strandi41 – 47Combined sources7
Beta strandi54 – 57Combined sources4
Beta strandi59 – 61Combined sources3
Beta strandi63 – 68Combined sources6
Helixi69 – 74Combined sources6
Helixi77 – 89Combined sources13
Helixi102 – 104Combined sources3
Turni105 – 108Combined sources4
Helixi119 – 128Combined sources10
Turni129 – 131Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C7KX-ray1.00A1-132[»]
1KUHX-ray1.60A1-132[»]
4HX3X-ray2.70A/C/E/G/I/K2-132[»]
ProteinModelPortaliP56406.
SMRiP56406.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56406.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M7 family.Curated

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiView protein in InterPro
IPR024079. MetalloPept_cat_dom.
IPR000013. Peptidase_M7.
PfamiView protein in Pfam
PF02031. Peptidase_M7. 1 hit.
PIRSFiPIRSF016573. Peptidase_M7. 1 hit.
PRINTSiPR00787. NEUTRALPTASE.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD016028. Peptidase_M7. 1 hit.

Sequencei

Sequence statusi: Complete.

P56406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TVTVTYDPSN APSFQQEIAN AAQIWNSSVR NVQLRAGGNA DFSYYEGNDS
60 70 80 90 100
RGSYAQTDGH GRGYIFLDYQ QNQQYDSTRV TAHETGHVLG LPDHYQGPCS
110 120 130
ELMSGGGPGP SCTNPYPNAQ ERSRVNALWA NG
Length:132
Mass (Da):14,376
Last modified:July 15, 1998 - v1
Checksum:i7CB988AFC2F0B1E4
GO

Sequence databases

PIRiS63978.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSNPA_STRCS
AccessioniPrimary (citable) accession number: P56406
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families