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Protein

Aquaporin-8

Gene

Aqp8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a water-specific channel; mercury-sensitive. It may have an important role in spermatogenesis, in fertilization, and in the secretion of pancreatic juice and saliva.

GO - Molecular functioni

  • glycerol channel activity Source: GO_Central
  • water channel activity Source: RGD

GO - Biological processi

  • canalicular bile acid transport Source: RGD
  • cellular water homeostasis Source: GO_Central
  • ion transmembrane transport Source: GO_Central
  • water transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-8
Short name:
AQP-8
Gene namesi
Name:Aqp8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2146. Aqp8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3838CytoplasmicSequence analysisAdd
BLAST
Transmembranei39 – 5921HelicalSequence analysisAdd
BLAST
Topological domaini60 – 8627ExtracellularSequence analysisAdd
BLAST
Transmembranei87 – 10721HelicalSequence analysisAdd
BLAST
Topological domaini108 – 1092CytoplasmicSequence analysis
Transmembranei110 – 13021HelicalSequence analysisAdd
BLAST
Topological domaini131 – 15828ExtracellularSequence analysisAdd
BLAST
Transmembranei159 – 17921HelicalSequence analysisAdd
BLAST
Topological domaini180 – 1856CytoplasmicSequence analysis
Transmembranei186 – 20621HelicalSequence analysisAdd
BLAST
Topological domaini207 – 23024ExtracellularSequence analysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence analysisAdd
BLAST
Topological domaini252 – 26312CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intracellular canaliculus Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Aquaporin-8PRO_0000063963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP56405.
PRIDEiP56405.

Expressioni

Tissue specificityi

Highly expressed in sperm, pancreas and liver. Some expression has been found in salivary gland and absorptive colonic epithelial cells.

Gene expression databases

GenevisibleiP56405. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019939.

Structurei

3D structure databases

ProteinModelPortaliP56405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi94 – 963NPA 1
Motifi212 – 2143NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
HOVERGENiHBG100478.
InParanoidiP56405.
KOiK09869.
OMAiEKFWNAS.
OrthoDBiEOG7N8ZWD.
PhylomeDBiP56405.
TreeFamiTF312940.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023277. Aquaporin_8.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02020. AQUAPORIN8.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGEQTPMCS MDLREIKGKE TNMADSYHGM SWYEQYIQPC VVELLGSALF
60 70 80 90 100
IFIGCLSVIE NSPNTGLLQP ALAHGLALGL IIATLGNISG GHFNPAVSLA
110 120 130 140 150
VTLVGGLKTM LLIPYWVSQL FGGMIGAALA KVVSPEERFW NASGAAFAIV
160 170 180 190 200
QEQEQVAEAL GVEIVMTMLL VLAVCMGAVN EKTMGPLAPF SIGFSVIVDI
210 220 230 240 250
LAGGGISGAC MNPARAFGPA VMAGYWDFHW IYWLGPLLAG LFVGLLIRLF
260
IGDEKTRLIL KSR
Length:263
Mass (Da):28,055
Last modified:July 15, 1998 - v1
Checksum:iB34AC8ACFA596BD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005547 mRNA. Translation: BAA21918.1.
AF007775 mRNA. Translation: AAC53463.1.
BC081812 mRNA. Translation: AAH81812.1.
PIRiJC5622.
RefSeqiNP_062031.1. NM_019158.2.
UniGeneiRn.6315.

Genome annotation databases

GeneIDi29172.
KEGGirno:29172.
UCSCiRGD:2146. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005547 mRNA. Translation: BAA21918.1.
AF007775 mRNA. Translation: AAC53463.1.
BC081812 mRNA. Translation: AAH81812.1.
PIRiJC5622.
RefSeqiNP_062031.1. NM_019158.2.
UniGeneiRn.6315.

3D structure databases

ProteinModelPortaliP56405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019939.

Proteomic databases

PaxDbiP56405.
PRIDEiP56405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29172.
KEGGirno:29172.
UCSCiRGD:2146. rat.

Organism-specific databases

CTDi343.
RGDi2146. Aqp8.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
HOVERGENiHBG100478.
InParanoidiP56405.
KOiK09869.
OMAiEKFWNAS.
OrthoDBiEOG7N8ZWD.
PhylomeDBiP56405.
TreeFamiTF312940.

Miscellaneous databases

PROiP56405.

Gene expression databases

GenevisibleiP56405. RN.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR023277. Aquaporin_8.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR02020. AQUAPORIN8.
PR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional expression of a second new aquaporin abundantly expressed in testis."
    Ishibashi K., Kuwahara M., Kageyama Y., Tohsaka A., Marumo F., Sasaki S.
    Biochem. Biophys. Res. Commun. 237:714-718(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver and Pancreas.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiAQP8_RAT
AccessioniPrimary (citable) accession number: P56405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.