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P56402

- AQP2_MOUSE

UniProt

P56402 - AQP2_MOUSE

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Protein
Aquaporin-2
Gene
Aqp2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient By similarity.

GO - Molecular functioni

  1. glycerol transmembrane transporter activity Source: Ensembl
  2. water channel activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. actin filament depolymerization Source: Ensembl
  2. aging Source: Ensembl
  3. apoptotic process Source: Ensembl
  4. cell volume homeostasis Source: Ensembl
  5. cellular response to copper ion Source: Ensembl
  6. cellular response to mercury ion Source: Ensembl
  7. cellular response to water deprivation Source: MGI
  8. female pregnancy Source: Ensembl
  9. hyperosmotic response Source: Ensembl
  10. metanephric collecting duct development Source: UniProtKB
  11. positive regulation of calcium ion transport Source: Ensembl
  12. renal water transport Source: MGI
  13. response to calcium ion Source: Ensembl
  14. response to glucagon Source: Ensembl
  15. response to lipopolysaccharide Source: Ensembl
  16. response to lithium ion Source: Ensembl
  17. response to salt stress Source: Ensembl
  18. response to starvation Source: Ensembl
  19. water transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_213947. Regulation of water balance by renal Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-2
Short name:
AQP-2
Alternative name(s):
ADH water channel
Aquaporin-CD
Short name:
AQP-CD
Collecting duct water channel protein
WCH-CD
Water channel protein for renal collecting duct
Gene namesi
Name:Aqp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1096865. Aqp2.

Subcellular locationi

Apical cell membrane; Multi-pass membrane protein By similarity. Basolateral cell membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity. Golgi apparatustrans-Golgi network membrane; Multi-pass membrane protein By similarity
Note: Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei17 – 3418Helical; Reviewed prediction
Add
BLAST
Topological domaini35 – 406Extracellular Reviewed prediction
Transmembranei41 – 5919Helical; Reviewed prediction
Add
BLAST
Topological domaini60 – 8526Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei86 – 10722Helical; Reviewed prediction
Add
BLAST
Topological domaini108 – 12720Extracellular Reviewed prediction
Add
BLAST
Transmembranei128 – 14821Helical; Reviewed prediction
Add
BLAST
Topological domaini149 – 1568Cytoplasmic Reviewed prediction
Transmembranei157 – 17620Helical; Reviewed prediction
Add
BLAST
Topological domaini177 – 20226Extracellular Reviewed prediction
Add
BLAST
Transmembranei203 – 22422Helical; Reviewed prediction
Add
BLAST
Topological domaini225 – 27147Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. basolateral plasma membrane Source: UniProtKB-SubCell
  3. clathrin-coated vesicle Source: Ensembl
  4. cytoplasmic vesicle Source: MGI
  5. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  6. early endosome Source: Ensembl
  7. exocytic vesicle Source: Ensembl
  8. integral component of endosome membrane Source: MGI
  9. integral component of membrane Source: MGI
  10. integral component of plasma membrane Source: MGI
  11. lysosome Source: Ensembl
  12. membrane Source: MGI
  13. plasma membrane Source: MGI
  14. protein complex Source: Ensembl
  15. recycling endosome Source: MGI
  16. recycling endosome membrane Source: MGI
  17. rough endoplasmic reticulum Source: Ensembl
  18. trans-Golgi network Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Aquaporin-2
PRO_0000063936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP56402.
PRIDEiP56402.

PTM databases

PhosphoSiteiP56402.

Expressioni

Tissue specificityi

Expressed in kidney. Expressed in a radial pattern from the cortex through the outer medulla into the inner medulla. Higher levels in the inner medulla. Located in tubules suggestive of collecting ducts.2 Publications

Inductioni

Increased levels on water deprivation.1 Publication

Gene expression databases

BgeeiP56402.
CleanExiMM_AQP2.
GenevestigatoriP56402.

Interactioni

Protein-protein interaction databases

BioGridi198171. 2 interactions.
DIPiDIP-58509N.

Structurei

3D structure databases

ProteinModelPortaliP56402.
SMRiP56402. Positions 3-239.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 703NPA 1
Motifi184 – 1863NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0580.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiP56402.
KOiK09865.
OMAiHEITPAN.
OrthoDBiEOG7N8ZWD.
PhylomeDBiP56402.
TreeFamiTF312940.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56402-1 [UniParc]FASTAAdd to Basket

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MWELRSIAFS RAVLAEFLAT LLFVFFGLGS ALQWASSPPS VLQIAVAFGL    50
GIGTLVQALG HVSGAHINPA VTVACLVGCH VSFLRAAFYV AAQLLGAVAG 100
AAILHEITPV EIRGDLAVNA LHNNATAGQA VTVELFLTMQ LVLCIFASTD 150
ERRSDNLGSP ALSIGFSVTL GHLLGIYFTG CSMNPARSLA PAVVTGKFDD 200
HWVFWIGPLV GAVIGSLLYN YLLFPSTKSL QERLAVLKGL EPDTDWEERE 250
VRRRQSVELH SPQSLPRGSK A 271
Length:271
Mass (Da):28,965
Last modified:September 19, 2002 - v2
Checksum:i41601C37BFD11CBA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 102FS → YC in AAB71414. 1 Publication
Sequence conflicti213 – 2131V → I in AAB71414. 1 Publication
Sequence conflicti213 – 2131V → I in AAH19966. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020519 mRNA. Translation: AAB71414.1.
AF105336 Genomic DNA. Translation: AAD21017.1.
AY055468 Genomic DNA. Translation: AAL15462.1.
BC019966 mRNA. Translation: AAH19966.1.
CCDSiCCDS27822.1.
RefSeqiNP_033829.3. NM_009699.3.
UniGeneiMm.20206.

Genome annotation databases

EnsembliENSMUST00000023752; ENSMUSP00000023752; ENSMUSG00000023013.
GeneIDi11827.
KEGGimmu:11827.
UCSCiuc007xps.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020519 mRNA. Translation: AAB71414.1 .
AF105336 Genomic DNA. Translation: AAD21017.1 .
AY055468 Genomic DNA. Translation: AAL15462.1 .
BC019966 mRNA. Translation: AAH19966.1 .
CCDSi CCDS27822.1.
RefSeqi NP_033829.3. NM_009699.3.
UniGenei Mm.20206.

3D structure databases

ProteinModelPortali P56402.
SMRi P56402. Positions 3-239.
ModBasei Search...

Protein-protein interaction databases

BioGridi 198171. 2 interactions.
DIPi DIP-58509N.

PTM databases

PhosphoSitei P56402.

Proteomic databases

PaxDbi P56402.
PRIDEi P56402.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023752 ; ENSMUSP00000023752 ; ENSMUSG00000023013 .
GeneIDi 11827.
KEGGi mmu:11827.
UCSCi uc007xps.2. mouse.

Organism-specific databases

CTDi 359.
MGIi MGI:1096865. Aqp2.

Phylogenomic databases

eggNOGi COG0580.
HOGENOMi HOG000288286.
HOVERGENi HBG000312.
InParanoidi P56402.
KOi K09865.
OMAi HEITPAN.
OrthoDBi EOG7N8ZWD.
PhylomeDBi P56402.
TreeFami TF312940.

Enzyme and pathway databases

Reactomei REACT_213947. Regulation of water balance by renal Aquaporins.

Miscellaneous databases

ChiTaRSi AQP2. mouse.
NextBioi 279731.
PROi P56402.
SOURCEi Search...

Gene expression databases

Bgeei P56402.
CleanExi MM_AQP2.
Genevestigatori P56402.

Family and domain databases

Gene3Di 1.20.1080.10. 1 hit.
InterProi IPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view ]
PANTHERi PTHR19139. PTHR19139. 1 hit.
Pfami PF00230. MIP. 1 hit.
[Graphical view ]
PRINTSi PR00783. MINTRINSICP.
SUPFAMi SSF81338. SSF81338. 1 hit.
TIGRFAMsi TIGR00861. MIP. 1 hit.
PROSITEi PS00221. MIP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA and genomic cloning of mouse aquaporin-2: functional analysis of an orthologous mutant causing nephrogenic diabetes insipidus."
    Yang B., Ma T., Xu Z., Verkman A.S.
    Genomics 57:79-83(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INDUCTION, TISSUE SPECIFICITY, CHARACTERISTICS OF AN ANIMAL MODEL OF ANDI.
    Strain: C57BL/6J.
    Tissue: Kidney.
  2. "Renal principal cell-specific expression of green fluorescent protein in transgenic mice."
    Zharkikh L., Zhu X., Stricklett P.K., Kohan D.E., Chipman G., Breton S., Brown D., Nelson R.D.
    Am. J. Physiol. 283:F1351-F1364(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiAQP2_MOUSE
AccessioniPrimary (citable) accession number: P56402
Secondary accession number(s): Q8VCG5, Q9R232
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 19, 2002
Last modified: September 3, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In an animal model of nephrogenic diabetes insipidis autosomal (ANDI-AQP2-T126M), AQP2 is fully functional but is retained in the endoplasmic reticulum (ER).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi