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P56402

- AQP2_MOUSE

UniProt

P56402 - AQP2_MOUSE

Protein

Aquaporin-2

Gene

Aqp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (19 Sep 2002)
      Previous versions | rss
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    Functioni

    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.By similarity

    GO - Molecular functioni

    1. glycerol transmembrane transporter activity Source: Ensembl
    2. water channel activity Source: MGI

    GO - Biological processi

    1. actin filament depolymerization Source: Ensembl
    2. aging Source: Ensembl
    3. apoptotic process Source: Ensembl
    4. cellular response to copper ion Source: Ensembl
    5. cellular response to mercury ion Source: Ensembl
    6. cellular response to water deprivation Source: MGI
    7. cell volume homeostasis Source: Ensembl
    8. female pregnancy Source: Ensembl
    9. hyperosmotic response Source: Ensembl
    10. metanephric collecting duct development Source: UniProtKB
    11. positive regulation of calcium ion transport Source: Ensembl
    12. renal water transport Source: MGI
    13. response to calcium ion Source: Ensembl
    14. response to glucagon Source: Ensembl
    15. response to lipopolysaccharide Source: Ensembl
    16. response to lithium ion Source: Ensembl
    17. response to salt stress Source: Ensembl
    18. response to starvation Source: Ensembl
    19. water transport Source: MGI

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_213947. Regulation of water balance by renal Aquaporins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aquaporin-2
    Short name:
    AQP-2
    Alternative name(s):
    ADH water channel
    Aquaporin-CD
    Short name:
    AQP-CD
    Collecting duct water channel protein
    WCH-CD
    Water channel protein for renal collecting duct
    Gene namesi
    Name:Aqp2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1096865. Aqp2.

    Subcellular locationi

    Apical cell membrane By similarity; Multi-pass membrane protein By similarity. Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity. Cytoplasmic vesicle membrane By similarity; Multi-pass membrane protein By similarity. Golgi apparatustrans-Golgi network membrane By similarity; Multi-pass membrane protein By similarity
    Note: Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. basolateral plasma membrane Source: UniProtKB-SubCell
    3. clathrin-coated vesicle Source: Ensembl
    4. cytoplasmic vesicle Source: MGI
    5. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    6. early endosome Source: Ensembl
    7. exocytic vesicle Source: Ensembl
    8. integral component of endosome membrane Source: MGI
    9. integral component of membrane Source: MGI
    10. integral component of plasma membrane Source: MGI
    11. lysosome Source: Ensembl
    12. membrane Source: MGI
    13. plasma membrane Source: MGI
    14. protein complex Source: Ensembl
    15. recycling endosome Source: MGI
    16. recycling endosome membrane Source: MGI
    17. rough endoplasmic reticulum Source: Ensembl
    18. trans-Golgi network Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 271271Aquaporin-2PRO_0000063936Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP56402.
    PRIDEiP56402.

    PTM databases

    PhosphoSiteiP56402.

    Expressioni

    Tissue specificityi

    Expressed in kidney. Expressed in a radial pattern from the cortex through the outer medulla into the inner medulla. Higher levels in the inner medulla. Located in tubules suggestive of collecting ducts.2 Publications

    Inductioni

    Increased levels on water deprivation.1 Publication

    Gene expression databases

    BgeeiP56402.
    CleanExiMM_AQP2.
    GenevestigatoriP56402.

    Interactioni

    Protein-protein interaction databases

    BioGridi198171. 2 interactions.
    DIPiDIP-58509N.

    Structurei

    3D structure databases

    ProteinModelPortaliP56402.
    SMRiP56402. Positions 3-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini35 – 406ExtracellularSequence Analysis
    Topological domaini60 – 8526CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini108 – 12720ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini149 – 1568CytoplasmicSequence Analysis
    Topological domaini177 – 20226ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini225 – 27147CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3418HelicalSequence AnalysisAdd
    BLAST
    Transmembranei41 – 5919HelicalSequence AnalysisAdd
    BLAST
    Transmembranei86 – 10722HelicalSequence AnalysisAdd
    BLAST
    Transmembranei128 – 14821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei203 – 22422HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 703NPA 1
    Motifi184 – 1863NPA 2

    Domaini

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0580.
    HOGENOMiHOG000288286.
    HOVERGENiHBG000312.
    InParanoidiP56402.
    KOiK09865.
    OMAiHEITPAN.
    OrthoDBiEOG7N8ZWD.
    PhylomeDBiP56402.
    TreeFamiTF312940.

    Family and domain databases

    Gene3Di1.20.1080.10. 1 hit.
    InterProiIPR023271. Aquaporin-like.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view]
    PANTHERiPTHR19139. PTHR19139. 1 hit.
    PfamiPF00230. MIP. 1 hit.
    [Graphical view]
    PRINTSiPR00783. MINTRINSICP.
    SUPFAMiSSF81338. SSF81338. 1 hit.
    TIGRFAMsiTIGR00861. MIP. 1 hit.
    PROSITEiPS00221. MIP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P56402-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWELRSIAFS RAVLAEFLAT LLFVFFGLGS ALQWASSPPS VLQIAVAFGL    50
    GIGTLVQALG HVSGAHINPA VTVACLVGCH VSFLRAAFYV AAQLLGAVAG 100
    AAILHEITPV EIRGDLAVNA LHNNATAGQA VTVELFLTMQ LVLCIFASTD 150
    ERRSDNLGSP ALSIGFSVTL GHLLGIYFTG CSMNPARSLA PAVVTGKFDD 200
    HWVFWIGPLV GAVIGSLLYN YLLFPSTKSL QERLAVLKGL EPDTDWEERE 250
    VRRRQSVELH SPQSLPRGSK A 271
    Length:271
    Mass (Da):28,965
    Last modified:September 19, 2002 - v2
    Checksum:i41601C37BFD11CBA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 102FS → YC in AAB71414. (PubMed:10191086)Curated
    Sequence conflicti213 – 2131V → I in AAB71414. (PubMed:10191086)Curated
    Sequence conflicti213 – 2131V → I in AAH19966. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020519 mRNA. Translation: AAB71414.1.
    AF105336 Genomic DNA. Translation: AAD21017.1.
    AY055468 Genomic DNA. Translation: AAL15462.1.
    BC019966 mRNA. Translation: AAH19966.1.
    CCDSiCCDS27822.1.
    RefSeqiNP_033829.3. NM_009699.3.
    UniGeneiMm.20206.

    Genome annotation databases

    EnsembliENSMUST00000023752; ENSMUSP00000023752; ENSMUSG00000023013.
    GeneIDi11827.
    KEGGimmu:11827.
    UCSCiuc007xps.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020519 mRNA. Translation: AAB71414.1 .
    AF105336 Genomic DNA. Translation: AAD21017.1 .
    AY055468 Genomic DNA. Translation: AAL15462.1 .
    BC019966 mRNA. Translation: AAH19966.1 .
    CCDSi CCDS27822.1.
    RefSeqi NP_033829.3. NM_009699.3.
    UniGenei Mm.20206.

    3D structure databases

    ProteinModelPortali P56402.
    SMRi P56402. Positions 3-239.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198171. 2 interactions.
    DIPi DIP-58509N.

    PTM databases

    PhosphoSitei P56402.

    Proteomic databases

    PaxDbi P56402.
    PRIDEi P56402.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023752 ; ENSMUSP00000023752 ; ENSMUSG00000023013 .
    GeneIDi 11827.
    KEGGi mmu:11827.
    UCSCi uc007xps.2. mouse.

    Organism-specific databases

    CTDi 359.
    MGIi MGI:1096865. Aqp2.

    Phylogenomic databases

    eggNOGi COG0580.
    HOGENOMi HOG000288286.
    HOVERGENi HBG000312.
    InParanoidi P56402.
    KOi K09865.
    OMAi HEITPAN.
    OrthoDBi EOG7N8ZWD.
    PhylomeDBi P56402.
    TreeFami TF312940.

    Enzyme and pathway databases

    Reactomei REACT_213947. Regulation of water balance by renal Aquaporins.

    Miscellaneous databases

    ChiTaRSi AQP2. mouse.
    NextBioi 279731.
    PROi P56402.
    SOURCEi Search...

    Gene expression databases

    Bgeei P56402.
    CleanExi MM_AQP2.
    Genevestigatori P56402.

    Family and domain databases

    Gene3Di 1.20.1080.10. 1 hit.
    InterProi IPR023271. Aquaporin-like.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view ]
    PANTHERi PTHR19139. PTHR19139. 1 hit.
    Pfami PF00230. MIP. 1 hit.
    [Graphical view ]
    PRINTSi PR00783. MINTRINSICP.
    SUPFAMi SSF81338. SSF81338. 1 hit.
    TIGRFAMsi TIGR00861. MIP. 1 hit.
    PROSITEi PS00221. MIP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA and genomic cloning of mouse aquaporin-2: functional analysis of an orthologous mutant causing nephrogenic diabetes insipidus."
      Yang B., Ma T., Xu Z., Verkman A.S.
      Genomics 57:79-83(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INDUCTION, TISSUE SPECIFICITY, CHARACTERISTICS OF AN ANIMAL MODEL OF ANDI.
      Strain: C57BL/6J.
      Tissue: Kidney.
    2. "Renal principal cell-specific expression of green fluorescent protein in transgenic mice."
      Zharkikh L., Zhu X., Stricklett P.K., Kohan D.E., Chipman G., Breton S., Brown D., Nelson R.D.
      Am. J. Physiol. 283:F1351-F1364(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: 129/SvJ.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiAQP2_MOUSE
    AccessioniPrimary (citable) accession number: P56402
    Secondary accession number(s): Q8VCG5, Q9R232
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: September 19, 2002
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In an animal model of nephrogenic diabetes insipidis autosomal (ANDI-AQP2-T126M), AQP2 is fully functional but is retained in the endoplasmic reticulum (ER).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3