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Protein

Aquaporin-2

Gene

Aqp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to copper ion Source: MGI
  • cellular response to mercury ion Source: MGI
  • cellular response to water deprivation Source: MGI
  • cellular water homeostasis Source: GO_Central
  • glycerol transport Source: MGI
  • ion transmembrane transport Source: GO_Central
  • metanephric collecting duct development Source: UniProtKB
  • renal water transport Source: MGI
  • water transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_327080. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-2
Short name:
AQP-2
Alternative name(s):
ADH water channel
Aquaporin-CD
Short name:
AQP-CD
Collecting duct water channel protein
WCH-CD
Water channel protein for renal collecting duct
Gene namesi
Name:Aqp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1096865. Aqp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei17 – 3418HelicalSequence AnalysisAdd
BLAST
Topological domaini35 – 406ExtracellularSequence Analysis
Transmembranei41 – 5919HelicalSequence AnalysisAdd
BLAST
Topological domaini60 – 8526CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei86 – 10722HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 12720ExtracellularSequence AnalysisAdd
BLAST
Transmembranei128 – 14821HelicalSequence AnalysisAdd
BLAST
Topological domaini149 – 1568CytoplasmicSequence Analysis
Transmembranei157 – 17620HelicalSequence AnalysisAdd
BLAST
Topological domaini177 – 20226ExtracellularSequence AnalysisAdd
BLAST
Transmembranei203 – 22422HelicalSequence AnalysisAdd
BLAST
Topological domaini225 – 27147CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • basolateral plasma membrane Source: UniProtKB-SubCell
  • cytoplasmic vesicle Source: MGI
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: UniProtKB-SubCell
  • integral component of endosome membrane Source: MGI
  • integral component of membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • membrane Source: MGI
  • plasma membrane Source: MGI
  • recycling endosome Source: MGI
  • recycling endosome membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Aquaporin-2PRO_0000063936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP56402.
PRIDEiP56402.

PTM databases

PhosphoSiteiP56402.

Expressioni

Tissue specificityi

Expressed in kidney. Expressed in a radial pattern from the cortex through the outer medulla into the inner medulla. Higher levels in the inner medulla. Located in tubules suggestive of collecting ducts.2 Publications

Inductioni

Increased levels on water deprivation.1 Publication

Gene expression databases

BgeeiP56402.
CleanExiMM_AQP2.
ExpressionAtlasiP56402. baseline and differential.
GenevisibleiP56402. MM.

Interactioni

Protein-protein interaction databases

BioGridi198171. 2 interactions.
DIPiDIP-58509N.
STRINGi10090.ENSMUSP00000023752.

Structurei

3D structure databases

ProteinModelPortaliP56402.
SMRiP56402. Positions 5-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 703NPA 1
Motifi184 – 1863NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0580.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiP56402.
KOiK09865.
OMAiMMWELRS.
OrthoDBiEOG7N8ZWD.
PhylomeDBiP56402.
TreeFamiTF312940.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56402-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWELRSIAFS RAVLAEFLAT LLFVFFGLGS ALQWASSPPS VLQIAVAFGL
60 70 80 90 100
GIGTLVQALG HVSGAHINPA VTVACLVGCH VSFLRAAFYV AAQLLGAVAG
110 120 130 140 150
AAILHEITPV EIRGDLAVNA LHNNATAGQA VTVELFLTMQ LVLCIFASTD
160 170 180 190 200
ERRSDNLGSP ALSIGFSVTL GHLLGIYFTG CSMNPARSLA PAVVTGKFDD
210 220 230 240 250
HWVFWIGPLV GAVIGSLLYN YLLFPSTKSL QERLAVLKGL EPDTDWEERE
260 270
VRRRQSVELH SPQSLPRGSK A
Length:271
Mass (Da):28,965
Last modified:September 19, 2002 - v2
Checksum:i41601C37BFD11CBA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 102FS → YC in AAB71414 (PubMed:10191086).Curated
Sequence conflicti213 – 2131V → I in AAB71414 (PubMed:10191086).Curated
Sequence conflicti213 – 2131V → I in AAH19966 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020519 mRNA. Translation: AAB71414.1.
AF105336 Genomic DNA. Translation: AAD21017.1.
AY055468 Genomic DNA. Translation: AAL15462.1.
BC019966 mRNA. Translation: AAH19966.1.
CCDSiCCDS27822.1.
RefSeqiNP_033829.3. NM_009699.3.
UniGeneiMm.20206.

Genome annotation databases

EnsembliENSMUST00000023752; ENSMUSP00000023752; ENSMUSG00000023013.
GeneIDi11827.
KEGGimmu:11827.
UCSCiuc007xps.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020519 mRNA. Translation: AAB71414.1.
AF105336 Genomic DNA. Translation: AAD21017.1.
AY055468 Genomic DNA. Translation: AAL15462.1.
BC019966 mRNA. Translation: AAH19966.1.
CCDSiCCDS27822.1.
RefSeqiNP_033829.3. NM_009699.3.
UniGeneiMm.20206.

3D structure databases

ProteinModelPortaliP56402.
SMRiP56402. Positions 5-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198171. 2 interactions.
DIPiDIP-58509N.
STRINGi10090.ENSMUSP00000023752.

PTM databases

PhosphoSiteiP56402.

Proteomic databases

PaxDbiP56402.
PRIDEiP56402.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023752; ENSMUSP00000023752; ENSMUSG00000023013.
GeneIDi11827.
KEGGimmu:11827.
UCSCiuc007xps.2. mouse.

Organism-specific databases

CTDi359.
MGIiMGI:1096865. Aqp2.

Phylogenomic databases

eggNOGiCOG0580.
GeneTreeiENSGT00760000119223.
HOGENOMiHOG000288286.
HOVERGENiHBG000312.
InParanoidiP56402.
KOiK09865.
OMAiMMWELRS.
OrthoDBiEOG7N8ZWD.
PhylomeDBiP56402.
TreeFamiTF312940.

Enzyme and pathway databases

ReactomeiREACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_327080. Passive transport by Aquaporins.

Miscellaneous databases

ChiTaRSiAqp2. mouse.
NextBioi279731.
PROiP56402.
SOURCEiSearch...

Gene expression databases

BgeeiP56402.
CleanExiMM_AQP2.
ExpressionAtlasiP56402. baseline and differential.
GenevisibleiP56402. MM.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA and genomic cloning of mouse aquaporin-2: functional analysis of an orthologous mutant causing nephrogenic diabetes insipidus."
    Yang B., Ma T., Xu Z., Verkman A.S.
    Genomics 57:79-83(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INDUCTION, TISSUE SPECIFICITY, CHARACTERISTICS OF AN ANIMAL MODEL OF ANDI.
    Strain: C57BL/6J.
    Tissue: Kidney.
  2. "Renal principal cell-specific expression of green fluorescent protein in transgenic mice."
    Zharkikh L., Zhu X., Stricklett P.K., Kohan D.E., Chipman G., Breton S., Brown D., Nelson R.D.
    Am. J. Physiol. 283:F1351-F1364(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiAQP2_MOUSE
AccessioniPrimary (citable) accession number: P56402
Secondary accession number(s): Q8VCG5, Q9R232
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 19, 2002
Last modified: July 22, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In an animal model of nephrogenic diabetes insipidis autosomal (ANDI-AQP2-T126M), AQP2 is fully functional but is retained in the endoplasmic reticulum (ER).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.