P56402 (AQP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin-2
Short name=AQP-2

Alternative name(s):
ADH water channel
Aquaporin-CD
Short name=AQP-CD
Collecting duct water channel protein
WCH-CD
Water channel protein for renal collecting duct

Gene names

Name:

Aqp2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient By similarity.
Subcellular location	Apical cell membrane; Multi-pass membrane protein By similarity. Basolateral cell membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity. Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein By similarity. Note: Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated By similarity.
Tissue specificity	Expressed in kidney. Expressed in a radial pattern from the cortex through the outer medulla into the inner medulla. Higher levels in the inner medulla. Located in tubules suggestive of collecting ducts. Ref.1 Ref.2
Induction	Increased levels on water deprivation. Ref.1
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Post-translational modification	Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent By similarity.
Miscellaneous	In an animal model of nephrogenic diabetes insipidis autosomal (ANDI-AQP2-T126M), AQP2 is fully functional but is retained in the endoplasmic reticulum (ER).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Cytoplasmic vesicle Golgi apparatus Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament depolymerization Inferred from electronic annotation. Source: Compara aging Inferred from electronic annotation. Source: Compara apoptotic process Inferred from electronic annotation. Source: Compara cell volume homeostasis Inferred from electronic annotation. Source: Compara cellular response to copper ion Inferred from electronic annotation. Source: Compara cellular response to mercury ion Inferred from electronic annotation. Source: Compara cellular response to water deprivation Inferred from direct assay Ref.1. Source: MGI female pregnancy Inferred from electronic annotation. Source: Compara hyperosmotic response Inferred from electronic annotation. Source: Compara metanephric collecting duct development Inferred from expression pattern PubMed 18618131. Source: UniProtKB positive regulation of calcium ion transport Inferred from electronic annotation. Source: Compara renal water transport Inferred from mutant phenotype PubMed 3184310. Source: MGI response to calcium ion Inferred from electronic annotation. Source: Compara response to glucagon stimulus Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to lithium ion Inferred from electronic annotation. Source: Compara response to salt stress Inferred from electronic annotation. Source: Compara response to starvation Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin-coated vesicle Inferred from electronic annotation. Source: Compara cytoplasmic vesicle Inferred from direct assay PubMed 18505797. Source: MGI cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome Inferred from electronic annotation. Source: Compara exocytic vesicle Inferred from electronic annotation. Source: Compara integral to endosome membrane Inferred by curator Ref.1. Source: MGI integral to plasma membrane Inferred by curator Ref.1. Source: MGI lysosome Inferred from electronic annotation. Source: Compara protein complex Inferred from electronic annotation. Source: Compara recycling endosome membrane Inferred by curator Ref.1. Source: MGI rough endoplasmic reticulum Inferred from electronic annotation. Source: Compara trans-Golgi network Inferred from electronic annotation. Source: Compara
Molecular_function	glycerol transmembrane transporter activity Inferred from electronic annotation. Source: Compara water channel activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Aquaporin-2		PRO_0000063936
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 34	18	Helical; Potential
	Topological domain	35 – 40	6	Extracellular Potential
	Transmembrane	41 – 59	19	Helical; Potential
	Topological domain	60 – 85	26	Cytoplasmic Potential
	Transmembrane	86 – 107	22	Helical; Potential
	Topological domain	108 – 127	20	Extracellular Potential
	Transmembrane	128 – 148	21	Helical; Potential
	Topological domain	149 – 156	8	Cytoplasmic Potential
	Transmembrane	157 – 176	20	Helical; Potential
	Topological domain	177 – 202	26	Extracellular Potential
	Transmembrane	203 – 224	22	Helical; Potential
	Topological domain	225 – 271	47	Cytoplasmic Potential
	Motif	68 – 70	3	NPA 1
	Motif	184 – 186	3	NPA 2
Amino acid modifications
	Modified residue	256	1	Phosphoserine By similarity
	Modified residue	261	1	Phosphoserine By similarity
	Modified residue	264	1	Phosphoserine By similarity
	Modified residue	269	1	Phosphoserine By similarity
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	9 – 10	2	FS → YC in AAB71414. Ref.1
	Sequence conflict	213	1	V → I in AAB71414. Ref.1
	Sequence conflict	213	1	V → I in AAH19966. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P56402 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 41601C37BFD11CBA	FASTA	271	28,965
	10 20 30 40 50 60 MWELRSIAFS RAVLAEFLAT LLFVFFGLGS ALQWASSPPS VLQIAVAFGL GIGTLVQALG 70 80 90 100 110 120 HVSGAHINPA VTVACLVGCH VSFLRAAFYV AAQLLGAVAG AAILHEITPV EIRGDLAVNA 130 140 150 160 170 180 LHNNATAGQA VTVELFLTMQ LVLCIFASTD ERRSDNLGSP ALSIGFSVTL GHLLGIYFTG 190 200 210 220 230 240 CSMNPARSLA PAVVTGKFDD HWVFWIGPLV GAVIGSLLYN YLLFPSTKSL QERLAVLKGL 250 260 270 EPDTDWEERE VRRRQSVELH SPQSLPRGSK A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA and genomic cloning of mouse aquaporin-2: functional analysis of an orthologous mutant causing nephrogenic diabetes insipidus." Yang B., Ma T., Xu Z., Verkman A.S. Genomics 57:79-83(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INDUCTION, TISSUE SPECIFICITY, CHARACTERISTICS OF AN ANIMAL MODEL OF ANDI. Strain: C57BL/6J. Tissue: Kidney.
[2]	"Renal principal cell-specific expression of green fluorescent protein in transgenic mice." Zharkikh L., Zhu X., Stricklett P.K., Kohan D.E., Chipman G., Breton S., Brown D., Nelson R.D. Am. J. Physiol. 283:F1351-F1364(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: 129/SvJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020519 mRNA. Translation: AAB71414.1. AF105336 Genomic DNA. Translation: AAD21017.1. AY055468 Genomic DNA. Translation: AAL15462.1. BC019966 mRNA. Translation: AAH19966.1.
IPI	IPI00113680.
RefSeq	NP_033829.3. NM_009699.3.
UniGene	Mm.20206.
3D structure databases
ProteinModelPortal	P56402.
SMR	P56402. Positions 3-239.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-58509N.
PTM databases
PhosphoSite	P56402.
Proteomic databases
PaxDb	P56402.
PRIDE	P56402.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023752; ENSMUSP00000023752; ENSMUSG00000023013.
GeneID	11827.
KEGG	mmu:11827.
UCSC	uc007xps.2. mouse.
Organism-specific databases
CTD	359.
MGI	MGI:1096865. Aqp2.
Phylogenomic databases
eggNOG	COG0580.
HOGENOM	HOG000288286.
HOVERGEN	HBG000312.
InParanoid	P56402.
KO	K09865.
OMA	ADWEERE.
OrthoDB	EOG4FJ89M.
Gene expression databases
ArrayExpress	P56402.
Bgee	P56402.
CleanEx	MM_AQP2.
Genevestigator	P56402.
GermOnline	ENSMUSG00000023013. Mus musculus.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AQP2. mouse.
NextBio	279731.
SOURCE	Search...

Entry information

Entry name

AQP2_MOUSE

Accession

Primary (citable) accession number: P56402
Secondary accession number(s): Q8VCG5, Q9R232

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 19, 2002
Last modified:	May 29, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program