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P56399

- UBP5_MOUSE

UniProt

P56399 - UBP5_MOUSE

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Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene

Usp5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991ZincBy similarity
Metal bindingi202 – 2021ZincBy similarity
Binding sitei209 – 2091SubstrateBy similarity
Metal bindingi219 – 2191ZincBy similarity
Metal bindingi232 – 2321ZincBy similarity
Binding sitei259 – 2591SubstrateBy similarity
Binding sitei261 – 2611Substrate; via carbonyl oxygenBy similarity
Binding sitei264 – 2641SubstrateBy similarity
Active sitei335 – 3351NucleophilePROSITE-ProRule annotation
Active sitei818 – 8181Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri197 – 26973UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. omega peptidase activity Source: InterPro
  3. ubiquitinyl hydrolase activity Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
  2. protein K48-linked deubiquitination Source: Ensembl
  3. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 5
Isopeptidase T
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene namesi
Name:Usp5
Synonyms:Isot
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1347343. Usp5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 858857Ubiquitin carboxyl-terminal hydrolase 5PRO_0000080624Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Disulfide bondi195 ↔ 816By similarity
Modified residuei623 – 6231Phosphothreonine1 Publication
Modified residuei783 – 7831PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP56399.
PaxDbiP56399.
PRIDEiP56399.

PTM databases

PhosphoSiteiP56399.

Expressioni

Gene expression databases

BgeeiP56399.
CleanExiMM_USP5.
ExpressionAtlasiP56399. baseline and differential.
GenevestigatoriP56399.

Interactioni

Subunit structurei

Interacts with TRIML1.1 Publication

Protein-protein interaction databases

BioGridi204425. 2 interactions.
IntActiP56399. 1 interaction.
MINTiMINT-220667.
STRINGi10090.ENSMUSP00000041299.

Structurei

3D structure databases

ProteinModelPortaliP56399.
SMRiP56399. Positions 1-858.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini326 – 856531USPAdd
BLAST
Domaini654 – 69542UBA 1PROSITE-ProRule annotationAdd
BLAST
Domaini722 – 76241UBA 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 2244Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri197 – 26973UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5207.
GeneTreeiENSGT00390000000874.
HOGENOMiHOG000162311.
HOVERGENiHBG002833.
InParanoidiP56399.
KOiK11836.
OMAiDDMVEDP.
OrthoDBiEOG7CNZF3.
PhylomeDBiP56399.
TreeFamiTF300576.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56399-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF
60 70 80 90 100
LGFGKQYVER HFNKTGQRVY LHLRRTRRPK EEDTSAGTGD PPRKKPTRLA
110 120 130 140 150
IGVEGGFDLT EDKFEFDEDV KIVILPDYLE IARDGLGGLP DIVRDRVTSA
160 170 180 190 200
VEALLSADSA SRKQEVQAWD GEVRQVSKHA FNLKQLDNPA RIPPCGWKCS
210 220 230 240 250
KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET GYPLAVKLGT
260 270 280 290 300
ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM
310 320 330 340 350
NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF
360 370 380 390 400
QRKYVDKLEK IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPALESGDGE
410 420 430 440 450
QVPEQKEVQD GIAPRMFKAL IGKGHPEFST NRQQDAQEFF LHLINMVERN
460 470 480 490 500
CRSSENPNEV FRFLVEEKIK CLATEKVKYT QRVDYIMQLP VPMDAALNKE
510 520 530 540 550
ELLEYEEKKR QAEEEKVPLP ELVRAQVPFS SCLEAYGAPE QVDDFWSTAL
560 570 580 590 600
QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS
610 620 630 640 650
QLRGTGLQPG EEELPDIAPP LVTPDEPKGS LGFYGNEDED SFCSPHFSSP
660 670 680 690 700
TSPMLDESVI IQLVEMGFPM DACRKAVYYT GNSGAEAAMN WVMSHMDDPD
710 720 730 740 750
FANPLILPGS SGPGSTSAAA DPPPEDCVTT IVSMGFSRDQ ALKALRATNN
760 770 780 790 800
SLERAVDWIF SHIDDLDAEA AMDISEGRSA AESISESVPV GPKVRDGPGK
810 820 830 840 850
YQLFAFISHM GTSTMCGHYV CHIKKEGRWV IYNDQKVCAS EKPPKDLGYI

YFYQRVVS
Length:858
Mass (Da):95,833
Last modified:July 15, 1998 - v1
Checksum:iE1F31A0C92687CA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA. Translation: AAC36015.1.
BC066993 mRNA. Translation: AAH66993.1.
CCDSiCCDS20531.1.
RefSeqiNP_038728.1. NM_013700.1.
UniGeneiMm.3571.

Genome annotation databases

EnsembliENSMUST00000047510; ENSMUSP00000041299; ENSMUSG00000038429.
GeneIDi22225.
KEGGimmu:22225.
UCSCiuc009dsa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA. Translation: AAC36015.1 .
BC066993 mRNA. Translation: AAH66993.1 .
CCDSi CCDS20531.1.
RefSeqi NP_038728.1. NM_013700.1.
UniGenei Mm.3571.

3D structure databases

ProteinModelPortali P56399.
SMRi P56399. Positions 1-858.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204425. 2 interactions.
IntActi P56399. 1 interaction.
MINTi MINT-220667.
STRINGi 10090.ENSMUSP00000041299.

Protein family/group databases

MEROPSi C19.001.

PTM databases

PhosphoSitei P56399.

Proteomic databases

MaxQBi P56399.
PaxDbi P56399.
PRIDEi P56399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047510 ; ENSMUSP00000041299 ; ENSMUSG00000038429 .
GeneIDi 22225.
KEGGi mmu:22225.
UCSCi uc009dsa.1. mouse.

Organism-specific databases

CTDi 8078.
MGIi MGI:1347343. Usp5.

Phylogenomic databases

eggNOGi COG5207.
GeneTreei ENSGT00390000000874.
HOGENOMi HOG000162311.
HOVERGENi HBG002833.
InParanoidi P56399.
KOi K11836.
OMAi DDMVEDP.
OrthoDBi EOG7CNZF3.
PhylomeDBi P56399.
TreeFami TF300576.

Miscellaneous databases

NextBioi 302251.
PROi P56399.
SOURCEi Search...

Gene expression databases

Bgeei P56399.
CleanExi MM_USP5.
ExpressionAtlasi P56399. baseline and differential.
Genevestigatori P56399.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PIRSFi PIRSF016308. UBP. 1 hit.
SMARTi SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
PROSITEi PS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "Characterization and potential function of a novel pre-implantation embryo-specific RING finger protein: TRIML1."
    Tian L., Wu X., Lin Y., Liu Z., Xiong F., Han Z., Zhou Y., Zeng Q., Wang Y., Deng J., Chen H.
    Mol. Reprod. Dev. 76:656-664(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIML1.

Entry informationi

Entry nameiUBP5_MOUSE
AccessioniPrimary (citable) accession number: P56399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: November 26, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3