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P56399

- UBP5_MOUSE

UniProt

P56399 - UBP5_MOUSE

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Protein
Ubiquitin carboxyl-terminal hydrolase 5
Gene
Usp5, Isot
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Cofactori

Zinc.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991Zinc By similarity
Metal bindingi202 – 2021Zinc By similarity
Binding sitei209 – 2091Substrate By similarity
Metal bindingi219 – 2191Zinc By similarity
Metal bindingi232 – 2321Zinc By similarity
Binding sitei259 – 2591Substrate By similarity
Binding sitei261 – 2611Substrate; via carbonyl oxygen By similarity
Binding sitei264 – 2641Substrate By similarity
Active sitei335 – 3351Nucleophile By similarity
Active sitei818 – 8181Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri197 – 26973UBP-type
Add
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. omega peptidase activity Source: InterPro
  3. protein binding Source: UniProtKB
  4. ubiquitinyl hydrolase activity Source: InterPro
  5. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
  2. protein K48-linked deubiquitination Source: Ensembl
  3. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 5
Isopeptidase T
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene namesi
Name:Usp5
Synonyms:Isot
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1347343. Usp5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 858857Ubiquitin carboxyl-terminal hydrolase 5
PRO_0000080624Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Disulfide bondi195 ↔ 816 By similarity
Modified residuei623 – 6231Phosphothreonine1 Publication
Modified residuei783 – 7831Phosphoserine By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP56399.
PaxDbiP56399.
PRIDEiP56399.

PTM databases

PhosphoSiteiP56399.

Expressioni

Gene expression databases

ArrayExpressiP56399.
BgeeiP56399.
CleanExiMM_USP5.
GenevestigatoriP56399.

Interactioni

Subunit structurei

Interacts with TRIML1.1 Publication

Protein-protein interaction databases

BioGridi204425. 2 interactions.
IntActiP56399. 1 interaction.
MINTiMINT-220667.
STRINGi10090.ENSMUSP00000041299.

Structurei

3D structure databases

ProteinModelPortaliP56399.
SMRiP56399. Positions 1-858.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini326 – 856531USP
Add
BLAST
Domaini654 – 69542UBA 1
Add
BLAST
Domaini722 – 76241UBA 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 2244Substrate binding By similarity

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 USP domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5207.
GeneTreeiENSGT00390000000874.
HOGENOMiHOG000162311.
HOVERGENiHBG002833.
InParanoidiP56399.
KOiK11836.
OMAiDDMVEDP.
OrthoDBiEOG7CNZF3.
PhylomeDBiP56399.
TreeFamiTF300576.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFiPIRSF016308. UBP. 1 hit.
SMARTiSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56399-1 [UniParc]FASTAAdd to Basket

« Hide

MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF    50
LGFGKQYVER HFNKTGQRVY LHLRRTRRPK EEDTSAGTGD PPRKKPTRLA 100
IGVEGGFDLT EDKFEFDEDV KIVILPDYLE IARDGLGGLP DIVRDRVTSA 150
VEALLSADSA SRKQEVQAWD GEVRQVSKHA FNLKQLDNPA RIPPCGWKCS 200
KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET GYPLAVKLGT 250
ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM 300
NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF 350
QRKYVDKLEK IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPALESGDGE 400
QVPEQKEVQD GIAPRMFKAL IGKGHPEFST NRQQDAQEFF LHLINMVERN 450
CRSSENPNEV FRFLVEEKIK CLATEKVKYT QRVDYIMQLP VPMDAALNKE 500
ELLEYEEKKR QAEEEKVPLP ELVRAQVPFS SCLEAYGAPE QVDDFWSTAL 550
QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS 600
QLRGTGLQPG EEELPDIAPP LVTPDEPKGS LGFYGNEDED SFCSPHFSSP 650
TSPMLDESVI IQLVEMGFPM DACRKAVYYT GNSGAEAAMN WVMSHMDDPD 700
FANPLILPGS SGPGSTSAAA DPPPEDCVTT IVSMGFSRDQ ALKALRATNN 750
SLERAVDWIF SHIDDLDAEA AMDISEGRSA AESISESVPV GPKVRDGPGK 800
YQLFAFISHM GTSTMCGHYV CHIKKEGRWV IYNDQKVCAS EKPPKDLGYI 850
YFYQRVVS 858
Length:858
Mass (Da):95,833
Last modified:July 15, 1998 - v1
Checksum:iE1F31A0C92687CA8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC002397 Genomic DNA. Translation: AAC36015.1.
BC066993 mRNA. Translation: AAH66993.1.
CCDSiCCDS20531.1.
RefSeqiNP_038728.1. NM_013700.1.
UniGeneiMm.3571.

Genome annotation databases

EnsembliENSMUST00000047510; ENSMUSP00000041299; ENSMUSG00000038429.
GeneIDi22225.
KEGGimmu:22225.
UCSCiuc009dsa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC002397 Genomic DNA. Translation: AAC36015.1 .
BC066993 mRNA. Translation: AAH66993.1 .
CCDSi CCDS20531.1.
RefSeqi NP_038728.1. NM_013700.1.
UniGenei Mm.3571.

3D structure databases

ProteinModelPortali P56399.
SMRi P56399. Positions 1-858.
ModBasei Search...

Protein-protein interaction databases

BioGridi 204425. 2 interactions.
IntActi P56399. 1 interaction.
MINTi MINT-220667.
STRINGi 10090.ENSMUSP00000041299.

Protein family/group databases

MEROPSi C19.001.

PTM databases

PhosphoSitei P56399.

Proteomic databases

MaxQBi P56399.
PaxDbi P56399.
PRIDEi P56399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047510 ; ENSMUSP00000041299 ; ENSMUSG00000038429 .
GeneIDi 22225.
KEGGi mmu:22225.
UCSCi uc009dsa.1. mouse.

Organism-specific databases

CTDi 8078.
MGIi MGI:1347343. Usp5.

Phylogenomic databases

eggNOGi COG5207.
GeneTreei ENSGT00390000000874.
HOGENOMi HOG000162311.
HOVERGENi HBG002833.
InParanoidi P56399.
KOi K11836.
OMAi DDMVEDP.
OrthoDBi EOG7CNZF3.
PhylomeDBi P56399.
TreeFami TF300576.

Miscellaneous databases

ChiTaRSi USP5. mouse.
NextBioi 302251.
PROi P56399.
SOURCEi Search...

Gene expression databases

ArrayExpressi P56399.
Bgeei P56399.
CleanExi MM_USP5.
Genevestigatori P56399.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PIRSFi PIRSF016308. UBP. 1 hit.
SMARTi SM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
SUPFAMi SSF46934. SSF46934. 1 hit.
PROSITEi PS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "Characterization and potential function of a novel pre-implantation embryo-specific RING finger protein: TRIML1."
    Tian L., Wu X., Lin Y., Liu Z., Xiong F., Han Z., Zhou Y., Zeng Q., Wang Y., Deng J., Chen H.
    Mol. Reprod. Dev. 76:656-664(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIML1.

Entry informationi

Entry nameiUBP5_MOUSE
AccessioniPrimary (citable) accession number: P56399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: September 3, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi