Ubiquitin carboxyl-terminal hydrolase 5

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P56399 (UBP5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 5
Isopeptidase T
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5

Gene names

Name:	Usp5
Synonyms:	Isot

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Cofactor	Zinc.
Subunit structure	Interacts with TRIML1. Ref.4
Sequence similarities	Belongs to the peptidase C19 family. Contains 2 UBA domains. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara protein K48-linked deubiquitination Inferred from electronic annotation. Source: Compara ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW omega peptidase activity Inferred from electronic annotation. Source: InterPro ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 858

858

Ubiquitin carboxyl-terminal hydrolase 5

PRO_0000080624

Regions

Domain

654 – 695

UBA 1

Domain

722 – 762

UBA 2

Zinc finger

197 – 269

UBP-type

Region

221 – 224

Substrate binding By similarity

Sites

Active site

335

Nucleophile By similarity

Active site

818

Proton acceptor By similarity

Metal binding

199

Zinc By similarity

Metal binding

202

Zinc By similarity

Metal binding

219

Zinc By similarity

Metal binding

232

Zinc By similarity

Binding site

209

Substrate By similarity

Binding site

259

Substrate By similarity

Binding site

261

Substrate; via carbonyl oxygen By similarity

Binding site

264

Substrate By similarity

Amino acid modifications

Modified residue

623

Phosphothreonine Ref.3

Modified residue

783

Phosphoserine By similarity

Disulfide bond

195 ↔ 816

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P56399 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: E1F31A0C92687CA8
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FASTA

858

95,833

        10         20         30         40         50         60 
MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF LGFGKQYVER 

        70         80         90        100        110        120 
HFNKTGQRVY LHLRRTRRPK EEDTSAGTGD PPRKKPTRLA IGVEGGFDLT EDKFEFDEDV 

       130        140        150        160        170        180 
KIVILPDYLE IARDGLGGLP DIVRDRVTSA VEALLSADSA SRKQEVQAWD GEVRQVSKHA 

       190        200        210        220        230        240 
FNLKQLDNPA RIPPCGWKCS KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET 

       250        260        270        280        290        300 
GYPLAVKLGT ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM 

       310        320        330        340        350        360 
NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF QRKYVDKLEK 

       370        380        390        400        410        420 
IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPALESGDGE QVPEQKEVQD GIAPRMFKAL 

       430        440        450        460        470        480 
IGKGHPEFST NRQQDAQEFF LHLINMVERN CRSSENPNEV FRFLVEEKIK CLATEKVKYT 

       490        500        510        520        530        540 
QRVDYIMQLP VPMDAALNKE ELLEYEEKKR QAEEEKVPLP ELVRAQVPFS SCLEAYGAPE 

       550        560        570        580        590        600 
QVDDFWSTAL QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS 

       610        620        630        640        650        660 
QLRGTGLQPG EEELPDIAPP LVTPDEPKGS LGFYGNEDED SFCSPHFSSP TSPMLDESVI 

       670        680        690        700        710        720 
IQLVEMGFPM DACRKAVYYT GNSGAEAAMN WVMSHMDDPD FANPLILPGS SGPGSTSAAA 

       730        740        750        760        770        780 
DPPPEDCVTT IVSMGFSRDQ ALKALRATNN SLERAVDWIF SHIDDLDAEA AMDISEGRSA 

       790        800        810        820        830        840 
AESISESVPV GPKVRDGPGK YQLFAFISHM GTSTMCGHYV CHIKKEGRWV IYNDQKVCAS 

       850 
EKPPKDLGYI YFYQRVVS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-623, MASS SPECTROMETRY. Tissue: Embryonic brain.
[4]	"Characterization and potential function of a novel pre-implantation embryo-specific RING finger protein: TRIML1." Tian L., Wu X., Lin Y., Liu Z., Xiong F., Han Z., Zhou Y., Zeng Q., Wang Y., Deng J., Chen H. Mol. Reprod. Dev. 76:656-664(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TRIML1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002397 Genomic DNA. Translation: AAC36015.1. BC066993 mRNA. Translation: AAH66993.1.
IPI	IPI00113214.
RefSeq	NP_038728.1. NM_013700.1.
UniGene	Mm.3571.
3D structure databases
ProteinModelPortal	P56399.
SMR	P56399. Positions 1-858.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-220667.
STRING	10090.ENSMUSP00000041299.
Protein family/group databases
MEROPS	C19.001.
PTM databases
PhosphoSite	P56399.
Proteomic databases
PaxDb	P56399.
PRIDE	P56399.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047510; ENSMUSP00000041299; ENSMUSG00000038429.
GeneID	22225.
KEGG	mmu:22225.
UCSC	uc009dsa.1. mouse.
Organism-specific databases
CTD	8078.
MGI	MGI:1347343. Usp5.
Phylogenomic databases
eggNOG	COG5207.
GeneTree	ENSGT00390000000874.
HOGENOM	HOG000162311.
HOVERGEN	HBG002833.
InParanoid	P56399.
KO	K11836.
OMA	DDMVEDP.
Gene expression databases
ArrayExpress	P56399.
Bgee	P56399.
CleanEx	MM_USP5.
Genevestigator	P56399.
GermOnline	ENSMUSG00000038429. Mus musculus.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR009060. UBA-like. IPR000449. UBA/transl_elong_EF1B_N. IPR015940. UBA/transl_elong_EF1B_N_euk. IPR016652. Ubiquitinyl_hydrolase. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00627. UBA. 2 hits. PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PIRSF	PIRSF016308. UBP. 1 hit.
SMART	SM00165. UBA. 2 hits. SM00290. ZnF_UBP. 1 hit. [Graphical view]
SUPFAM	SSF46934. UBA_like. 1 hit.
PROSITE	PS50030. UBA. 2 hits. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	USP5. mouse.
NextBio	302251.
SOURCE	Search...

Entry information

Entry name

UBP5_MOUSE

Accession

Primary (citable) accession number: P56399

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents