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P56399

- UBP5_MOUSE

UniProt

P56399 - UBP5_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene

Usp5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Cofactori

    Zinc.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi199 – 1991ZincBy similarity
    Metal bindingi202 – 2021ZincBy similarity
    Binding sitei209 – 2091SubstrateBy similarity
    Metal bindingi219 – 2191ZincBy similarity
    Metal bindingi232 – 2321ZincBy similarity
    Binding sitei259 – 2591SubstrateBy similarity
    Binding sitei261 – 2611Substrate; via carbonyl oxygenBy similarity
    Binding sitei264 – 2641SubstrateBy similarity
    Active sitei335 – 3351NucleophilePROSITE-ProRule annotation
    Active sitei818 – 8181Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri197 – 26973UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. omega peptidase activity Source: InterPro
    3. protein binding Source: UniProtKB
    4. ubiquitinyl hydrolase activity Source: InterPro
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: Ensembl
    2. protein K48-linked deubiquitination Source: Ensembl
    3. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 5
    Isopeptidase T
    Ubiquitin thioesterase 5
    Ubiquitin-specific-processing protease 5
    Gene namesi
    Name:Usp5
    Synonyms:Isot
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1347343. Usp5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 858857Ubiquitin carboxyl-terminal hydrolase 5PRO_0000080624Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Disulfide bondi195 ↔ 816By similarity
    Modified residuei623 – 6231Phosphothreonine1 Publication
    Modified residuei783 – 7831PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Phosphoprotein

    Proteomic databases

    MaxQBiP56399.
    PaxDbiP56399.
    PRIDEiP56399.

    PTM databases

    PhosphoSiteiP56399.

    Expressioni

    Gene expression databases

    ArrayExpressiP56399.
    BgeeiP56399.
    CleanExiMM_USP5.
    GenevestigatoriP56399.

    Interactioni

    Subunit structurei

    Interacts with TRIML1.1 Publication

    Protein-protein interaction databases

    BioGridi204425. 2 interactions.
    IntActiP56399. 1 interaction.
    MINTiMINT-220667.
    STRINGi10090.ENSMUSP00000041299.

    Structurei

    3D structure databases

    ProteinModelPortaliP56399.
    SMRiP56399. Positions 1-858.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini326 – 856531USPAdd
    BLAST
    Domaini654 – 69542UBA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini722 – 76241UBA 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni221 – 2244Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 2 UBA domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri197 – 26973UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5207.
    GeneTreeiENSGT00390000000874.
    HOGENOMiHOG000162311.
    HOVERGENiHBG002833.
    InParanoidiP56399.
    KOiK11836.
    OMAiDDMVEDP.
    OrthoDBiEOG7CNZF3.
    PhylomeDBiP56399.
    TreeFamiTF300576.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR016652. Ubiquitinyl_hydrolase.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00627. UBA. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016308. UBP. 1 hit.
    SMARTiSM00165. UBA. 2 hits.
    SM00290. ZnF_UBP. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    PROSITEiPS50030. UBA. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P56399-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF    50
    LGFGKQYVER HFNKTGQRVY LHLRRTRRPK EEDTSAGTGD PPRKKPTRLA 100
    IGVEGGFDLT EDKFEFDEDV KIVILPDYLE IARDGLGGLP DIVRDRVTSA 150
    VEALLSADSA SRKQEVQAWD GEVRQVSKHA FNLKQLDNPA RIPPCGWKCS 200
    KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET GYPLAVKLGT 250
    ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM 300
    NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF 350
    QRKYVDKLEK IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPALESGDGE 400
    QVPEQKEVQD GIAPRMFKAL IGKGHPEFST NRQQDAQEFF LHLINMVERN 450
    CRSSENPNEV FRFLVEEKIK CLATEKVKYT QRVDYIMQLP VPMDAALNKE 500
    ELLEYEEKKR QAEEEKVPLP ELVRAQVPFS SCLEAYGAPE QVDDFWSTAL 550
    QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS 600
    QLRGTGLQPG EEELPDIAPP LVTPDEPKGS LGFYGNEDED SFCSPHFSSP 650
    TSPMLDESVI IQLVEMGFPM DACRKAVYYT GNSGAEAAMN WVMSHMDDPD 700
    FANPLILPGS SGPGSTSAAA DPPPEDCVTT IVSMGFSRDQ ALKALRATNN 750
    SLERAVDWIF SHIDDLDAEA AMDISEGRSA AESISESVPV GPKVRDGPGK 800
    YQLFAFISHM GTSTMCGHYV CHIKKEGRWV IYNDQKVCAS EKPPKDLGYI 850
    YFYQRVVS 858
    Length:858
    Mass (Da):95,833
    Last modified:July 15, 1998 - v1
    Checksum:iE1F31A0C92687CA8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002397 Genomic DNA. Translation: AAC36015.1.
    BC066993 mRNA. Translation: AAH66993.1.
    CCDSiCCDS20531.1.
    RefSeqiNP_038728.1. NM_013700.1.
    UniGeneiMm.3571.

    Genome annotation databases

    EnsembliENSMUST00000047510; ENSMUSP00000041299; ENSMUSG00000038429.
    GeneIDi22225.
    KEGGimmu:22225.
    UCSCiuc009dsa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002397 Genomic DNA. Translation: AAC36015.1 .
    BC066993 mRNA. Translation: AAH66993.1 .
    CCDSi CCDS20531.1.
    RefSeqi NP_038728.1. NM_013700.1.
    UniGenei Mm.3571.

    3D structure databases

    ProteinModelPortali P56399.
    SMRi P56399. Positions 1-858.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204425. 2 interactions.
    IntActi P56399. 1 interaction.
    MINTi MINT-220667.
    STRINGi 10090.ENSMUSP00000041299.

    Protein family/group databases

    MEROPSi C19.001.

    PTM databases

    PhosphoSitei P56399.

    Proteomic databases

    MaxQBi P56399.
    PaxDbi P56399.
    PRIDEi P56399.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047510 ; ENSMUSP00000041299 ; ENSMUSG00000038429 .
    GeneIDi 22225.
    KEGGi mmu:22225.
    UCSCi uc009dsa.1. mouse.

    Organism-specific databases

    CTDi 8078.
    MGIi MGI:1347343. Usp5.

    Phylogenomic databases

    eggNOGi COG5207.
    GeneTreei ENSGT00390000000874.
    HOGENOMi HOG000162311.
    HOVERGENi HBG002833.
    InParanoidi P56399.
    KOi K11836.
    OMAi DDMVEDP.
    OrthoDBi EOG7CNZF3.
    PhylomeDBi P56399.
    TreeFami TF300576.

    Miscellaneous databases

    ChiTaRSi USP5. mouse.
    NextBioi 302251.
    PROi P56399.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P56399.
    Bgeei P56399.
    CleanExi MM_USP5.
    Genevestigatori P56399.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    IPR016652. Ubiquitinyl_hydrolase.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00627. UBA. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016308. UBP. 1 hit.
    SMARTi SM00165. UBA. 2 hits.
    SM00290. ZnF_UBP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    PROSITEi PS50030. UBA. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    4. "Characterization and potential function of a novel pre-implantation embryo-specific RING finger protein: TRIML1."
      Tian L., Wu X., Lin Y., Liu Z., Xiong F., Han Z., Zhou Y., Zeng Q., Wang Y., Deng J., Chen H.
      Mol. Reprod. Dev. 76:656-664(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TRIML1.

    Entry informationi

    Entry nameiUBP5_MOUSE
    AccessioniPrimary (citable) accession number: P56399
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3