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P56399 (UBP5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 5

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 5
Isopeptidase T
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene names
Name:Usp5
Synonyms:Isot
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Cofactor

Zinc.

Subunit structure

Interacts with TRIML1. Ref.4

Sequence similarities

Belongs to the peptidase C19 family.

Contains 2 UBA domains.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 858857Ubiquitin carboxyl-terminal hydrolase 5
PRO_0000080624

Regions

Domain326 – 856531USP
Domain654 – 69542UBA 1
Domain722 – 76241UBA 2
Zinc finger197 – 26973UBP-type
Region221 – 2244Substrate binding By similarity

Sites

Active site3351Nucleophile By similarity
Active site8181Proton acceptor By similarity
Metal binding1991Zinc By similarity
Metal binding2021Zinc By similarity
Metal binding2191Zinc By similarity
Metal binding2321Zinc By similarity
Binding site2091Substrate By similarity
Binding site2591Substrate By similarity
Binding site2611Substrate; via carbonyl oxygen By similarity
Binding site2641Substrate By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue6231Phosphothreonine Ref.3
Modified residue7831Phosphoserine By similarity
Disulfide bond195 ↔ 816 By similarity

Sequences

Sequence LengthMass (Da)Tools
P56399 [UniParc].

Last modified July 15, 1998. Version 1.
Checksum: E1F31A0C92687CA8

FASTA85895,833
        10         20         30         40         50         60 
MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF LGFGKQYVER 

        70         80         90        100        110        120 
HFNKTGQRVY LHLRRTRRPK EEDTSAGTGD PPRKKPTRLA IGVEGGFDLT EDKFEFDEDV 

       130        140        150        160        170        180 
KIVILPDYLE IARDGLGGLP DIVRDRVTSA VEALLSADSA SRKQEVQAWD GEVRQVSKHA 

       190        200        210        220        230        240 
FNLKQLDNPA RIPPCGWKCS KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET 

       250        260        270        280        290        300 
GYPLAVKLGT ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM 

       310        320        330        340        350        360 
NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF QRKYVDKLEK 

       370        380        390        400        410        420 
IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPALESGDGE QVPEQKEVQD GIAPRMFKAL 

       430        440        450        460        470        480 
IGKGHPEFST NRQQDAQEFF LHLINMVERN CRSSENPNEV FRFLVEEKIK CLATEKVKYT 

       490        500        510        520        530        540 
QRVDYIMQLP VPMDAALNKE ELLEYEEKKR QAEEEKVPLP ELVRAQVPFS SCLEAYGAPE 

       550        560        570        580        590        600 
QVDDFWSTAL QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS 

       610        620        630        640        650        660 
QLRGTGLQPG EEELPDIAPP LVTPDEPKGS LGFYGNEDED SFCSPHFSSP TSPMLDESVI 

       670        680        690        700        710        720 
IQLVEMGFPM DACRKAVYYT GNSGAEAAMN WVMSHMDDPD FANPLILPGS SGPGSTSAAA 

       730        740        750        760        770        780 
DPPPEDCVTT IVSMGFSRDQ ALKALRATNN SLERAVDWIF SHIDDLDAEA AMDISEGRSA 

       790        800        810        820        830        840 
AESISESVPV GPKVRDGPGK YQLFAFISHM GTSTMCGHYV CHIKKEGRWV IYNDQKVCAS 

       850 
EKPPKDLGYI YFYQRVVS 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-623, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[4]"Characterization and potential function of a novel pre-implantation embryo-specific RING finger protein: TRIML1."
Tian L., Wu X., Lin Y., Liu Z., Xiong F., Han Z., Zhou Y., Zeng Q., Wang Y., Deng J., Chen H.
Mol. Reprod. Dev. 76:656-664(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRIML1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC002397 Genomic DNA. Translation: AAC36015.1.
BC066993 mRNA. Translation: AAH66993.1.
CCDSCCDS20531.1.
RefSeqNP_038728.1. NM_013700.1.
UniGeneMm.3571.

3D structure databases

ProteinModelPortalP56399.
SMRP56399. Positions 1-858.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204425. 2 interactions.
IntActP56399. 1 interaction.
MINTMINT-220667.
STRING10090.ENSMUSP00000041299.

Protein family/group databases

MEROPSC19.001.

PTM databases

PhosphoSiteP56399.

Proteomic databases

MaxQBP56399.
PaxDbP56399.
PRIDEP56399.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047510; ENSMUSP00000041299; ENSMUSG00000038429.
GeneID22225.
KEGGmmu:22225.
UCSCuc009dsa.1. mouse.

Organism-specific databases

CTD8078.
MGIMGI:1347343. Usp5.

Phylogenomic databases

eggNOGCOG5207.
GeneTreeENSGT00390000000874.
HOGENOMHOG000162311.
HOVERGENHBG002833.
InParanoidP56399.
KOK11836.
OMADDMVEDP.
OrthoDBEOG7CNZF3.
PhylomeDBP56399.
TreeFamTF300576.

Gene expression databases

ArrayExpressP56399.
BgeeP56399.
CleanExMM_USP5.
GenevestigatorP56399.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFPIRSF016308. UBP. 1 hit.
SMARTSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS50030. UBA. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSP5. mouse.
NextBio302251.
PROP56399.
SOURCESearch...

Entry information

Entry nameUBP5_MOUSE
AccessionPrimary (citable) accession number: P56399
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1998
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot