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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei198 – 1981ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:trpS
Ordered Locus Names:HP_1253
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Tryptophan--tRNA ligasePRO_0000136637Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi85962.HP1253.

Structurei

3D structure databases

ProteinModelPortaliP56396.
SMRiP56396. Positions 4-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 209"HIGH" region
Motifi195 – 1995"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0180.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG686NJQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P56396-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHKKRVFSGI QPTGQIHLGN YLGAIKHWVE MQDEYENLFC VVNSHAITLP
60 70 80 90 100
IDPAFLKSQS YELVKLLLAC GIDPKQSGLF IQSEVDEHPA LAWLLNCQVS
110 120 130 140 150
MGEMQRMTQF KDKSLKNPKS VNVGLFNYPI LMASDILLYQ SDLVPVGEDQ
160 170 180 190 200
KQHLELTRNI AEKFNRDFGN CFKVPEPLIA KVGARVMGLD DPKVKMSKSH
210 220 230 240 250
QGANHAIFLL DEPDIIVKKI KKAATDSMGV IAFDEKREGI FNLLNIYMLL
260 270 280 290 300
SNESPENIEE RFKNKGYGDF KKELAEVMIQ ALKPIQERYK EISDDEVKAI
310 320
LNGGAEKARP LARATYQKAK ELMGLI
Length:326
Mass (Da):36,836
Last modified:May 30, 2000 - v2
Checksum:iFB352D7B685E2F80
GO

Sequence cautioni

The sequence AAD08297.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08297.1. Different initiation.
PIRiE64676.
RefSeqiNP_208045.1. NC_000915.1.
YP_006935173.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08297; AAD08297; HP_1253.
GeneIDi13870460.
899025.
KEGGiheo:C694_06475.
hpy:HP1253.
PATRICi20593883. VBIHelPyl33062_1312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08297.1. Different initiation.
PIRiE64676.
RefSeqiNP_208045.1. NC_000915.1.
YP_006935173.1. NC_018939.1.

3D structure databases

ProteinModelPortaliP56396.
SMRiP56396. Positions 4-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP1253.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08297; AAD08297; HP_1253.
GeneIDi13870460.
899025.
KEGGiheo:C694_06475.
hpy:HP1253.
PATRICi20593883. VBIHelPyl33062_1312.

Phylogenomic databases

eggNOGiCOG0180.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG686NJQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiSYW_HELPY
AccessioniPrimary (citable) accession number: P56396
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: January 7, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.