Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P56396 (SYW_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:HP_1253
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_B

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140_B

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140_B.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAD08297.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Tryptophan--tRNA ligase HAMAP-Rule MF_00140_B
PRO_0000136637

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140_B
Motif195 – 1995"KMSKS" region HAMAP-Rule MF_00140_B

Sites

Binding site1981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P56396 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: FB352D7B685E2F80

FASTA32636,836
        10         20         30         40         50         60 
MHKKRVFSGI QPTGQIHLGN YLGAIKHWVE MQDEYENLFC VVNSHAITLP IDPAFLKSQS 

        70         80         90        100        110        120 
YELVKLLLAC GIDPKQSGLF IQSEVDEHPA LAWLLNCQVS MGEMQRMTQF KDKSLKNPKS 

       130        140        150        160        170        180 
VNVGLFNYPI LMASDILLYQ SDLVPVGEDQ KQHLELTRNI AEKFNRDFGN CFKVPEPLIA 

       190        200        210        220        230        240 
KVGARVMGLD DPKVKMSKSH QGANHAIFLL DEPDIIVKKI KKAATDSMGV IAFDEKREGI 

       250        260        270        280        290        300 
FNLLNIYMLL SNESPENIEE RFKNKGYGDF KKELAEVMIQ ALKPIQERYK EISDDEVKAI 

       310        320 
LNGGAEKARP LARATYQKAK ELMGLI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08297.1. Different initiation.
PIRE64676.
RefSeqNP_208045.1. NC_000915.1.
YP_006935173.1. NC_018939.1.

3D structure databases

ProteinModelPortalP56396.
SMRP56396. Positions 4-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85962.HP1253.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08297; AAD08297; HP_1253.
GeneID13870460.
899025.
KEGGheo:C694_06475.
hpy:HP1253.
PATRIC20593883. VBIHelPyl33062_1312.

Phylogenomic databases

eggNOGCOG0180.
KOK01867.
OMAGWGQFKP.
OrthoDBEOG686NJQ.
ProtClustDBPRK00927.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_HELPY
AccessionPrimary (citable) accession number: P56396
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 30, 2000
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries