Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome b5

Gene

Cyb5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi68 – 681Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

  • enzyme binding Source: MGI
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • stearoyl-CoA 9-desaturase activity Source: MGI

GO - Biological processi

  • fatty acid metabolic process Source: MGI
  • response to cadmium ion Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:Cyb5a
Synonyms:Cyb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1926952. Cyb5a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei109 – 13123HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 134133Cytochrome b5PRO_0000166011Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei7 – 71N6-acetyllysineCombined sources
Modified residuei10 – 101N6-acetyllysineCombined sources
Modified residuei19 – 191N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP56395.
PaxDbiP56395.
PRIDEiP56395.
TopDownProteomicsiP56395.

2D gel databases

REPRODUCTION-2DPAGEIPI00230113.
P56395.
SWISS-2DPAGEP56395.

PTM databases

iPTMnetiP56395.
PhosphoSiteiP56395.

Expressioni

Gene expression databases

BgeeiP56395.
CleanExiMM_CYB5.
ExpressionAtlasiP56395. baseline and differential.
GenevisibleiP56395. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224948. 1 interaction.
IntActiP56395. 5 interactions.
MINTiMINT-1862489.
STRINGi10090.ENSMUSP00000124480.

Structurei

3D structure databases

ProteinModelPortaliP56395.
SMRiP56395. Positions 10-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 8577Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP56395.
OMAiWTNWLIP.
OrthoDBiEOG78H3W2.
PhylomeDBiP56395.
TreeFamiTF314537.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGQSDKDVK YYTLEEIQKH KDSKSTWVIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTYI IGELHPDDRS KIAKPSDTLI
110 120 130
TTVESNSSWW TNWVIPAISA LAVALMYRLY MAED
Length:134
Mass (Da):15,241
Last modified:January 23, 2007 - v2
Checksum:iC2540A68320107C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024341 mRNA. Translation: AAH24341.1.
CCDSiCCDS29385.1.
RefSeqiNP_080073.1. NM_025797.3.
UniGeneiMm.31018.

Genome annotation databases

EnsembliENSMUST00000160180; ENSMUSP00000124480; ENSMUSG00000024646.
GeneIDi109672.
KEGGimmu:109672.
UCSCiuc008fuw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024341 mRNA. Translation: AAH24341.1.
CCDSiCCDS29385.1.
RefSeqiNP_080073.1. NM_025797.3.
UniGeneiMm.31018.

3D structure databases

ProteinModelPortaliP56395.
SMRiP56395. Positions 10-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224948. 1 interaction.
IntActiP56395. 5 interactions.
MINTiMINT-1862489.
STRINGi10090.ENSMUSP00000124480.

PTM databases

iPTMnetiP56395.
PhosphoSiteiP56395.

2D gel databases

REPRODUCTION-2DPAGEIPI00230113.
P56395.
SWISS-2DPAGEP56395.

Proteomic databases

EPDiP56395.
PaxDbiP56395.
PRIDEiP56395.
TopDownProteomicsiP56395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000160180; ENSMUSP00000124480; ENSMUSG00000024646.
GeneIDi109672.
KEGGimmu:109672.
UCSCiuc008fuw.1. mouse.

Organism-specific databases

CTDi1528.
MGIiMGI:1926952. Cyb5a.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP56395.
OMAiWTNWLIP.
OrthoDBiEOG78H3W2.
PhylomeDBiP56395.
TreeFamiTF314537.

Enzyme and pathway databases

ReactomeiR-MMU-196836. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

PROiP56395.
SOURCEiSearch...

Gene expression databases

BgeeiP56395.
CleanExiMM_CYB5.
ExpressionAtlasiP56395. baseline and differential.
GenevisibleiP56395. MM.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  2. Bienvenut W.V.
    Submitted (JUL-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-10; 25-73 AND 78-89, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Liver.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-7; LYS-10 AND LYS-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCYB5_MOUSE
AccessioniPrimary (citable) accession number: P56395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.