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Protein

Cytochrome b5

Gene

Cyb5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi68 – 681Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

  • enzyme binding Source: MGI
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • stearoyl-CoA 9-desaturase activity Source: MGI

GO - Biological processi

  • fatty acid metabolic process Source: MGI
  • response to cadmium ion Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5
Gene namesi
Name:Cyb5a
Synonyms:Cyb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1926952. Cyb5a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei109 – 13123HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 134133Cytochrome b5PRO_0000166011Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei7 – 71N6-acetyllysineCombined sources
Modified residuei10 – 101N6-acetyllysineCombined sources
Modified residuei19 – 191N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP56395.
PaxDbiP56395.
PeptideAtlasiP56395.
PRIDEiP56395.
TopDownProteomicsiP56395.

2D gel databases

REPRODUCTION-2DPAGEIPI00230113.
P56395.
SWISS-2DPAGEP56395.

PTM databases

iPTMnetiP56395.
PhosphoSiteiP56395.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024646.
CleanExiMM_CYB5.
ExpressionAtlasiP56395. baseline and differential.
GenevisibleiP56395. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224948. 1 interaction.
IntActiP56395. 5 interactions.
MINTiMINT-1862489.
STRINGi10090.ENSMUSP00000124480.

Structurei

3D structure databases

ProteinModelPortaliP56395.
SMRiP56395. Positions 10-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 8577Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP56395.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
PhylomeDBiP56395.
TreeFamiTF314537.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P56395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGQSDKDVK YYTLEEIQKH KDSKSTWVIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTYI IGELHPDDRS KIAKPSDTLI
110 120 130
TTVESNSSWW TNWVIPAISA LAVALMYRLY MAED
Length:134
Mass (Da):15,241
Last modified:January 23, 2007 - v2
Checksum:iC2540A68320107C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024341 mRNA. Translation: AAH24341.1.
CCDSiCCDS29385.1.
RefSeqiNP_080073.1. NM_025797.3.
UniGeneiMm.31018.

Genome annotation databases

EnsembliENSMUST00000160180; ENSMUSP00000124480; ENSMUSG00000024646.
GeneIDi109672.
KEGGimmu:109672.
UCSCiuc008fuw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC024341 mRNA. Translation: AAH24341.1.
CCDSiCCDS29385.1.
RefSeqiNP_080073.1. NM_025797.3.
UniGeneiMm.31018.

3D structure databases

ProteinModelPortaliP56395.
SMRiP56395. Positions 10-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224948. 1 interaction.
IntActiP56395. 5 interactions.
MINTiMINT-1862489.
STRINGi10090.ENSMUSP00000124480.

PTM databases

iPTMnetiP56395.
PhosphoSiteiP56395.

2D gel databases

REPRODUCTION-2DPAGEIPI00230113.
P56395.
SWISS-2DPAGEP56395.

Proteomic databases

EPDiP56395.
PaxDbiP56395.
PeptideAtlasiP56395.
PRIDEiP56395.
TopDownProteomicsiP56395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000160180; ENSMUSP00000124480; ENSMUSG00000024646.
GeneIDi109672.
KEGGimmu:109672.
UCSCiuc008fuw.1. mouse.

Organism-specific databases

CTDi1528.
MGIiMGI:1926952. Cyb5a.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP56395.
OMAiFKSTWII.
OrthoDBiEOG091G17G8.
PhylomeDBiP56395.
TreeFamiTF314537.

Enzyme and pathway databases

ReactomeiR-MMU-196836. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

PROiP56395.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024646.
CleanExiMM_CYB5.
ExpressionAtlasiP56395. baseline and differential.
GenevisibleiP56395. MM.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5_MOUSE
AccessioniPrimary (citable) accession number: P56395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.