Reviewed, UniProtKB/Swiss-Prot P56395 (CYB5_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytochrome b5

Gene names

Name:	Cyb5a
Synonyms:	Cyb5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-5 double bond introduction during the C-5 desaturation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW fatty acid metabolic process Inferred by curator. Source: MGI transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Traceable author statement. Source: MGI mitochondrion Inferred from direct assay. Source: MGI
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro stearoyl-CoA 9-desaturase activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 134	133	Cytochrome b5		PRO_0000166011
Regions
	Transmembrane	109 – 131	23	Potential
	Domain	9 – 85	77	Cytochrome b5 heme-binding
Sites
	Metal binding	44	1	Iron (heme axial ligand) By similarity
	Metal binding	68	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.2
	Modified residue	19	1	N6-acetyllysine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P56395-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C2540A68320107C8	FASTA	134	15,241
	10 20 30 40 50 60 MAGQSDKDVK YYTLEEIQKH KDSKSTWVIL HHKVYDLTKF LEEHPGGEEV LREQAGGDAT 70 80 90 100 110 120 ENFEDVGHST DARELSKTYI IGELHPDDRS KIAKPSDTLI TTVESNSSWW TNWVIPAISA 130 LAVALMYRLY MAED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[2]	Bienvenut W.V. Submitted (JUL-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10; 25-73 AND 78-89, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Liver.
[3]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-19, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC024341 mRNA. Translation: AAH24341.1.
IPI	IPI00230113.
RefSeq	NP_080073.1.
UniGene	Mm.31018
3D structure databases
HSSP	HSSP built from PDB template 1AQA based on UniProtKB P00173.
SMR	P56395. Positions 6-99.
ModBase	Search...
PTM databases
PhosphoSite	P56395.
2-D gel databases
SWISS-2DPAGE	P56395.
REPRODUCTION-2DPAGE	P56395.
Proteomic databases
PRIDE	P56395.
Genome annotation databases
Ensembl	ENSMUSG00000024646. Mus musculus. [Contig view]
GeneID	109672.
KEGG	mmu:109672.
Organism-specific databases
MGI	MGI:1926952. Cyb5.
Phylogenomic databases
HOGENOM	P56395.
HOVERGEN	P56395.
OMA	P56395. QKHNHSK.
Gene expression databases
ArrayExpress	P56395.
Bgee	P56395.
CleanEx	MM_CYB5.
GermOnline	ENSMUSG00000024646. Mus musculus.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	362549.
SOURCE	Search...

Entry information

Entry name

CYB5_MOUSE

Accession

Primary (citable) accession number: P56395

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	May 5, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)