P56388 (CART_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cocaine- and amphetamine-regulated transcript protein

Cleaved into the following 3 chains:

1. CART(1-52)
2. CART(55-102)
3. CART(62-102)

Gene names

Name:	Cartpt
Synonyms:	Cart

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the CART family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Neuropeptide Neurotransmitter
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPKK activity Inferred from direct assay PubMed 15908120. Source: HGNC adult feeding behavior Inferred from direct assay PubMed 9590691. Source: HGNC cellular glucose homeostasis Inferred from sequence or structural similarity. Source: HGNC cellular response to starvation Inferred from direct assay PubMed 9590691. Source: HGNC circadian regulation of gene expression Inferred from sequence or structural similarity. Source: HGNC negative regulation of appetite Inferred from mutant phenotype PubMed 9590691. Source: HGNC negative regulation of bone resorption Inferred from genetic interaction PubMed 16614075. Source: HGNC negative regulation of glucagon secretion Inferred from electronic annotation. Source: Compara negative regulation of osteoclast differentiation Traceable author statement PubMed 16614075. Source: HGNC neuropeptide signaling pathway Traceable author statement Ref.1. Source: MGI positive regulation of blood pressure Inferred from sequence or structural similarity. Source: HGNC positive regulation of epinephrine secretion Inferred from sequence or structural similarity. Source: HGNC positive regulation of transmission of nerve impulse Inferred from sequence or structural similarity. Source: HGNC regulation of insulin secretion Inferred from electronic annotation. Source: Compara somatostatin secretion Inferred from electronic annotation. Source: Compara synaptic transmission Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from direct assay PubMed 9590691. Source: HGNC secretory granule Inferred from electronic annotation. Source: Compara
Molecular_function	neuropeptide hormone activity Traceable author statement Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P56388-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P56388-2)

The sequence of this isoform differs from the canonical sequence as follows:
     54-66: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 129	102	Cocaine- and amphetamine-regulated transcript protein		PRO_0000004436
	Peptide	28 – 79	52	CART(1-52) By similarity		PRO_0000004437
	Peptide	82 – 129	48	CART(55-102) By similarity		PRO_0000004438
	Peptide	89 – 129	41	CART(62-102) Potential		PRO_0000004439
Amino acid modifications
	Disulfide bond	95 ↔ 113		By similarity
	Disulfide bond	101 ↔ 121		By similarity
	Disulfide bond	115 ↔ 128		By similarity
Natural variations
	Alternative sequence	54 – 66	13	Missing in isoform Short.		VSP_000793

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 16, 2001. Version 2. Checksum: E4CA6CE70BEE6DF2	FASTA	129	14,285
	10 20 30 40 50 60 MESSRLRLLP LLGAALLLLL PLLGARAQED AELQPRALDI YSAVDDASHE KELPRRQLRA 70 80 90 100 110 120 PGAMLQIEAL QEVLKKLKSK RIPIYEKKYG QVPMCDAGEQ CAVRKGARIG KLCDCPRGTS CNSFLLKCL
	Isoform Short [UniParc]. Checksum: 9248CCBA89203754	FASTA	116	12,809
	10 20 30 40 50 60 MESSRLRLLP LLGAALLLLL PLLGARAQED AELQPRALDI YSAVDDASHE KELIEALQEV 70 80 90 100 110 LKKLKSKRIP IYEKKYGQVP MCDAGEQCAV RKGARIGKLC DCPRGTSCNS FLLKCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CART: from gene to function." Adams L.D., Gong W., Vechia S.D., Hunter R.G., Kuhar M.J. Brain Res. 848:137-140(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: 129/SvJ.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF148071 Genomic DNA. Translation: AAF24168.1. BC056431 mRNA. Translation: AAH56431.1.
IPI	IPI00113180. IPI00230430.
RefSeq	NP_038760.3. NM_013732.7.
UniGene	Mm.75498.
3D structure databases
ProteinModelPortal	P56388.
SMR	P56388. Positions 89-129.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000022150.
PTM databases
PhosphoSite	P56388.
Proteomic databases
PaxDb	P56388.
PRIDE	P56388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022150; ENSMUSP00000022150; ENSMUSG00000021647.
GeneID	27220.
KEGG	mmu:27220.
UCSC	uc007rpw.1. mouse.
Organism-specific databases
CTD	9607.
MGI	MGI:1351330. Cartpt.
Phylogenomic databases
eggNOG	NOG47455.
GeneTree	ENSGT00390000018319.
HOGENOM	HOG000111306.
HOVERGEN	HBG018929.
InParanoid	P56388.
OMA	SHEKELP.
OrthoDB	EOG4G1MJ1.
Gene expression databases
ArrayExpress	P56388.
Bgee	P56388.
CleanEx	MM_CARTPT.
Genevestigator	P56388.
GermOnline	ENSMUSG00000021647. Mus musculus.
Family and domain databases
Gene3D	4.10.40.30. 1 hit.
InterPro	IPR009106. CART. [Graphical view]
PANTHER	PTHR16655. PTHR16655. 1 hit.
Pfam	PF06373. CART. 1 hit. [Graphical view]
SUPFAM	SSF64546. CART. 1 hit.
ProtoNet	Search...
Other
NextBio	305124.
SOURCE	Search...

Entry information

Entry name

CART_MOUSE

Accession

Primary (citable) accession number: P56388
Secondary accession number(s): Q9QXZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 16, 2001
Last modified:	April 3, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program