Reviewed, UniProtKB/Swiss-Prot P56380 (AP4A_MOUSE)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
    EC=3.6.1.17
Alternative name(s):
    Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
      Short name=Diadenosine tetraphosphatase
      Short name=Ap4A hydrolase
      Short name=Ap4Aase
    Nucleoside diphosphate-linked moiety X motif 2
      Short name=Nudix motif 2

Gene names

Name:	Nudt2
Synonyms:	Apah1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4A to yield AMP and ATP. Plays a major role in maintaining homeostasis By similarity.
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP.
Cofactor	Divalent ions By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	induction of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 147	146	Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]		PRO_0000057103
Regions
	Motif	43 – 64	22	Nudix box
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Experimental info
	Sequence conflict	19	1	M → V in BAB31399. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P56380-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A634D19F1E8CCE34	FASTA	147	16,989
	10 20 30 40 50 60 MALRACGLII FRRHLIPKMD NSTIEFLLLQ ASDGIHHWTP PKGHVDPGEN DLETALRETR 70 80 90 100 110 120 EETGIEASQL TIIEGFRREL NYVARQKPKT VIYWLAEVKD YNVEIRLSQE HQAYRWLGLE 130 140 EACQLAQFKE MKATLQEGHQ FLCSTPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum and Tongue.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[3]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 5-12; 43-57; 61-77; 90-99 AND 116-129, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK009933 mRNA. Translation: BAB26592.1. AK018771 mRNA. Translation: BAB31399.1. BC025153 mRNA. Translation: AAH25153.1.
IPI	IPI00135345.
RefSeq	NP_079815.2.
UniGene	Mm.28574
3D structure databases
HSSP	HSSP built from PDB template 1KTG based on UniProtKB Q9U2M7.
SMR	P56380. Positions 1-147.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000028443. Mus musculus. [Contig view]
GeneID	66401.
KEGG	mmu:66401.
Organism-specific databases
MGI	MGI:1913651. Nudt2.
Phylogenomic databases
HOGENOM	P56380.
HOVERGEN	P56380.
OMA	P56380. HQFLCST.
Enzyme and pathway databases
BRENDA	3.6.1.17. 244.
Gene expression databases
ArrayExpress	P56380.
Bgee	P56380.
GermOnline	ENSMUSG00000028443. Mus musculus.
Family and domain databases
InterPro	IPR000086. NUDIX_hydrolase_core. IPR003565. Tetra_PHTase. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR01405. TETRPHPHTASE.
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	321571.
SOURCE	Search...

Entry information

Entry name

AP4A_MOUSE

Accession

Primary (citable) accession number: P56380
Secondary accession number(s): Q9D2U6, Q9D6V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)