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Protein

Probable translation initiation factor eIF-2B subunit gamma

Gene

tif223

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.By similarity

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: PomBase
  • nucleotidyltransferase activity Source: InterPro
  • translation initiation factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational initiation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor eIF-2B subunit gamma
Alternative name(s):
eIF-2B GDP-GTP exchange factor subunit gamma
Gene namesi
Name:tif223
ORF Names:SPAC4D7.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.09.
PomBaseiSPAC4D7.09. tif223.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation initiation factor 2B complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001560821 – 458Probable translation initiation factor eIF-2B subunit gammaAdd BLAST458

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56288.
PRIDEiP56288.

Interactioni

Subunit structurei

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.By similarity

Protein-protein interaction databases

BioGridi280036. 4 interactors.
DIPiDIP-61960N.
MINTiMINT-4691047.

Structurei

Secondary structure

1458
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 35Combined sources3
Beta strandi39 – 45Combined sources7
Turni52 – 54Combined sources3
Beta strandi58 – 60Combined sources3
Helixi62 – 64Combined sources3
Beta strandi66 – 71Combined sources6
Helixi74 – 82Combined sources9
Beta strandi87 – 92Combined sources6
Helixi97 – 105Combined sources9
Beta strandi112 – 117Combined sources6
Helixi128 – 134Combined sources7
Helixi136 – 138Combined sources3
Beta strandi141 – 147Combined sources7
Helixi156 – 166Combined sources11
Beta strandi169 – 176Combined sources8
Beta strandi196 – 199Combined sources4
Turni200 – 203Combined sources4
Beta strandi204 – 207Combined sources4
Beta strandi210 – 213Combined sources4
Beta strandi216 – 221Combined sources6
Turni222 – 226Combined sources5
Beta strandi230 – 245Combined sources16
Helixi247 – 249Combined sources3
Turni250 – 252Combined sources3
Beta strandi253 – 256Combined sources4
Turni261 – 266Combined sources6
Beta strandi315 – 319Combined sources5
Beta strandi321 – 323Combined sources3
Helixi332 – 338Combined sources7
Beta strandi353 – 360Combined sources8
Beta strandi373 – 376Combined sources4
Beta strandi390 – 393Combined sources4
Beta strandi407 – 410Combined sources4
Beta strandi424 – 427Combined sources4
Beta strandi441 – 443Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99E/F1-458[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP56288.
KOiK03241.
OMAiEEGCNIQ.
OrthoDBiEOG092C1UCY.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

P56288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLYEHAALP LASSPSILGP ISGGRNRGNI QLQSIPIEFQ AVVFAGFGNS
60 70 80 90 100
LYPLTGSDAL PKALLPIGNK PMLHYPLYWL EAAGFTSAIL ICMEEAEAHI
110 120 130 140 150
NAWLRSGYEG HMRIHVEAPT ILDDSKSSAD ALRAVSHLIK NDFVCLSCDS
160 170 180 190 200
IVGLPPIYGL DKFRLDNPSA LAVYSPVLKY EHITSQSKEI DAKQLIGIEE
210 220 230 240 250
KTSRLLYAKS SADVGSDFTF RMSLLWKHPR VTLNTNLSDA HIFVFKHWVI
260 270 280 290 300
DLIREKESIS SIRGDLIPYL VKCQYQKSFT VRENIQRFLS SPNNIDNYDG
310 320 330 340 350
GLSSQEIKIN ALIAKDGIIC SRANNLPNYF ELNKCIAKLT PEQRLVDVTV
360 370 380 390 400
SERALVGADC MVNEGTTIKD NSNIKKSIIG KNCVIGKGVV VSNSILMDNI
410 420 430 440 450
VVEDGVRLES CIVASGAQIG AKSKLRECEI GVDHRVEAGR IARGERLVDM

EKIETDMD
Length:458
Mass (Da):50,462
Last modified:April 18, 2012 - v2
Checksum:i242987543BE43021
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11281.2.
PIRiT38800.
RefSeqiNP_594962.2. NM_001020393.2.

Genome annotation databases

EnsemblFungiiSPAC4D7.09.1; SPAC4D7.09.1:pep; SPAC4D7.09.
GeneIDi2543622.
KEGGispo:SPAC4D7.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11281.2.
PIRiT38800.
RefSeqiNP_594962.2. NM_001020393.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99E/F1-458[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280036. 4 interactors.
DIPiDIP-61960N.
MINTiMINT-4691047.

Proteomic databases

MaxQBiP56288.
PRIDEiP56288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4D7.09.1; SPAC4D7.09.1:pep; SPAC4D7.09.
GeneIDi2543622.
KEGGispo:SPAC4D7.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.09.
PomBaseiSPAC4D7.09. tif223.

Phylogenomic databases

InParanoidiP56288.
KOiK03241.
OMAiEEGCNIQ.
OrthoDBiEOG092C1UCY.

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiP56288.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiEI2BG_SCHPO
AccessioniPrimary (citable) accession number: P56288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 18, 2012
Last modified: October 5, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.