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Protein

Probable translation initiation factor eIF-2B subunit gamma

Gene

tif223

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable translation initiation factor eIF-2B subunit gamma
Alternative name(s):
eIF-2B GDP-GTP exchange factor subunit gamma
Gene namesi
Name:tif223
ORF Names:SPAC4D7.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.09.
PomBaseiSPAC4D7.09. tif223.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • eukaryotic translation initiation factor 2B complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458Probable translation initiation factor eIF-2B subunit gammaPRO_0000156082Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei291 – 2911PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP56288.

Interactioni

Subunit structurei

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.By similarity

Protein-protein interaction databases

BioGridi280036. 4 interactions.
MINTiMINT-4691047.

Structurei

Secondary structure

1
458
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 353Combined sources
Beta strandi39 – 457Combined sources
Turni52 – 543Combined sources
Beta strandi58 – 603Combined sources
Helixi62 – 643Combined sources
Beta strandi66 – 716Combined sources
Helixi74 – 829Combined sources
Beta strandi87 – 926Combined sources
Helixi97 – 1059Combined sources
Beta strandi112 – 1176Combined sources
Helixi128 – 1347Combined sources
Helixi136 – 1383Combined sources
Beta strandi141 – 1477Combined sources
Helixi156 – 16611Combined sources
Beta strandi169 – 1768Combined sources
Beta strandi196 – 1994Combined sources
Turni200 – 2034Combined sources
Beta strandi204 – 2074Combined sources
Beta strandi210 – 2134Combined sources
Beta strandi216 – 2216Combined sources
Turni222 – 2265Combined sources
Beta strandi230 – 24516Combined sources
Helixi247 – 2493Combined sources
Turni250 – 2523Combined sources
Beta strandi253 – 2564Combined sources
Turni261 – 2666Combined sources
Beta strandi315 – 3195Combined sources
Beta strandi321 – 3233Combined sources
Helixi332 – 3387Combined sources
Beta strandi353 – 3608Combined sources
Beta strandi373 – 3764Combined sources
Beta strandi390 – 3934Combined sources
Beta strandi407 – 4104Combined sources
Beta strandi424 – 4274Combined sources
Beta strandi441 – 4433Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99E/F1-458[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP56288.
KOiK03241.
OMAiEEGCNIQ.
OrthoDBiEOG7008K9.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

P56288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLYEHAALP LASSPSILGP ISGGRNRGNI QLQSIPIEFQ AVVFAGFGNS
60 70 80 90 100
LYPLTGSDAL PKALLPIGNK PMLHYPLYWL EAAGFTSAIL ICMEEAEAHI
110 120 130 140 150
NAWLRSGYEG HMRIHVEAPT ILDDSKSSAD ALRAVSHLIK NDFVCLSCDS
160 170 180 190 200
IVGLPPIYGL DKFRLDNPSA LAVYSPVLKY EHITSQSKEI DAKQLIGIEE
210 220 230 240 250
KTSRLLYAKS SADVGSDFTF RMSLLWKHPR VTLNTNLSDA HIFVFKHWVI
260 270 280 290 300
DLIREKESIS SIRGDLIPYL VKCQYQKSFT VRENIQRFLS SPNNIDNYDG
310 320 330 340 350
GLSSQEIKIN ALIAKDGIIC SRANNLPNYF ELNKCIAKLT PEQRLVDVTV
360 370 380 390 400
SERALVGADC MVNEGTTIKD NSNIKKSIIG KNCVIGKGVV VSNSILMDNI
410 420 430 440 450
VVEDGVRLES CIVASGAQIG AKSKLRECEI GVDHRVEAGR IARGERLVDM

EKIETDMD
Length:458
Mass (Da):50,462
Last modified:April 18, 2012 - v2
Checksum:i242987543BE43021
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11281.2.
PIRiT38800.
RefSeqiNP_594962.2. NM_001020393.2.

Genome annotation databases

EnsemblFungiiSPAC4D7.09.1; SPAC4D7.09.1:pep; SPAC4D7.09.
GeneIDi2543622.
KEGGispo:SPAC4D7.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB11281.2.
PIRiT38800.
RefSeqiNP_594962.2. NM_001020393.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B04X-ray2.99E/F1-458[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280036. 4 interactions.
MINTiMINT-4691047.

Proteomic databases

MaxQBiP56288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4D7.09.1; SPAC4D7.09.1:pep; SPAC4D7.09.
GeneIDi2543622.
KEGGispo:SPAC4D7.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC4D7.09.
PomBaseiSPAC4D7.09. tif223.

Phylogenomic databases

InParanoidiP56288.
KOiK03241.
OMAiEEGCNIQ.
OrthoDBiEOG7008K9.

Enzyme and pathway databases

ReactomeiR-SPO-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiP56288.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Comparative functional genomics of the fission yeasts."
    Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
    , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
    Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiEI2BG_SCHPO
AccessioniPrimary (citable) accession number: P56288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 18, 2012
Last modified: June 8, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.